Objective: This study examined Herpes Simplex Virus (HSV) subclinical shedding in the genital tract of patients withgenital herpes (GH) or non-gonoccal urethritis (NGU). Method Swabs were collected after exposure to r...Objective: This study examined Herpes Simplex Virus (HSV) subclinical shedding in the genital tract of patients withgenital herpes (GH) or non-gonoccal urethritis (NGU). Method Swabs were collected after exposure to rash andgenital tract during GH relapse or remission on a weekly basisfor four to six weeks. NGU patients with negative chlamydiaand mycoplasma tests were also swabbed for a similarduration. All swabs underwent HSV DNA detection withquantitative PCR. Result: There was a significant difference in the rate ofasymptomatic HSV shedding in urinary tracts comparing GHand the control group and comparing NGU and the controlgroup (P<0.05). The rate of HSV shedding was 22%, 9.8%and 3.3% for GH, NGU and control groups respectively. Therate of HSV shedding was 21.7% (20/92) for patients withactive GH and 23% for those in remission. The HSV positiverate was significantly higher in the group with patients whohad more than six relapses within one year compared to thegroup of patients with less than six relapses.Conclusion: There is HSV subclinical shedding in theirgenital tract during active GH and remission. SubclinicalHSV shedding is more common in patients with more than sixGH relapses per year compared to GH patients with fewerrelapses. Approximately 9.9% of NGU patients with negativechlamydia mycoplasma testing was found to have subclinicalHSV infection.展开更多
An M6.5 earthquake occurred on August 3rd, 2014 in Ludian of Yunnan Province in China, causing severe casualty and economic loss. Local broadband waveform inversion with the CAP method demonstrates that the earthquake...An M6.5 earthquake occurred on August 3rd, 2014 in Ludian of Yunnan Province in China, causing severe casualty and economic loss. Local broadband waveform inversion with the CAP method demonstrates that the earthquake is a strike-slip event, with the strike along 70° and 160° for the two nodal planes respectively. However, the geological structure in the epicentral region is complicated with abundant active faults, and it is challenging to identify the seismogenic fault with the focal plane solutions due to nodal-plane ambiguity. We resolved the rupture directivity by measuring the difference between centroid location and hypocenter of the Ludian earthquake with the time shift from CAP inversion, and found that the nodal plane with the strike of 160° is the ruptured fault plane. Moreover, the rupture is found to propagate from northwest to southeast.展开更多
The dengue virus （DENV） is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigati...The dengue virus （DENV） is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope （E） gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype I strains, including 8 genotype I and 9 genotype V strains. Additionally, 6 genotype I strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype I strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype V strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.展开更多
基金This Project was supported by Medical and Health Care Scientific Research Fund of Guangdong Province(B1998147).
文摘Objective: This study examined Herpes Simplex Virus (HSV) subclinical shedding in the genital tract of patients withgenital herpes (GH) or non-gonoccal urethritis (NGU). Method Swabs were collected after exposure to rash andgenital tract during GH relapse or remission on a weekly basisfor four to six weeks. NGU patients with negative chlamydiaand mycoplasma tests were also swabbed for a similarduration. All swabs underwent HSV DNA detection withquantitative PCR. Result: There was a significant difference in the rate ofasymptomatic HSV shedding in urinary tracts comparing GHand the control group and comparing NGU and the controlgroup (P<0.05). The rate of HSV shedding was 22%, 9.8%and 3.3% for GH, NGU and control groups respectively. Therate of HSV shedding was 21.7% (20/92) for patients withactive GH and 23% for those in remission. The HSV positiverate was significantly higher in the group with patients whohad more than six relapses within one year compared to thegroup of patients with less than six relapses.Conclusion: There is HSV subclinical shedding in theirgenital tract during active GH and remission. SubclinicalHSV shedding is more common in patients with more than sixGH relapses per year compared to GH patients with fewerrelapses. Approximately 9.9% of NGU patients with negativechlamydia mycoplasma testing was found to have subclinicalHSV infection.
基金supported by the National Natural Science Foundation of China(Grant No.41274069)National Basic Research Program of China(Grant No.2014CB845901)
文摘An M6.5 earthquake occurred on August 3rd, 2014 in Ludian of Yunnan Province in China, causing severe casualty and economic loss. Local broadband waveform inversion with the CAP method demonstrates that the earthquake is a strike-slip event, with the strike along 70° and 160° for the two nodal planes respectively. However, the geological structure in the epicentral region is complicated with abundant active faults, and it is challenging to identify the seismogenic fault with the focal plane solutions due to nodal-plane ambiguity. We resolved the rupture directivity by measuring the difference between centroid location and hypocenter of the Ludian earthquake with the time shift from CAP inversion, and found that the nodal plane with the strike of 160° is the ruptured fault plane. Moreover, the rupture is found to propagate from northwest to southeast.
基金supported by the Guangdong Natural Science Foundation (No. S2012030006598)the Science & Technology Planning Project of Guangdong Province of China (No. 2013A020229007)the Innovative Program of the State Key Laboratory of Virology (No. 2016KF001)
文摘The dengue virus （DENV） is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope （E） gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype I strains, including 8 genotype I and 9 genotype V strains. Additionally, 6 genotype I strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype I strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype V strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.
