[Objective] The aim of this study was to reveal the distribution of rapeseed clubfoot disease in Hubei Province and to assess the yield loss caused by the pathogen, Plasmodiophora brassicae. [Method] Field surveys wer...[Objective] The aim of this study was to reveal the distribution of rapeseed clubfoot disease in Hubei Province and to assess the yield loss caused by the pathogen, Plasmodiophora brassicae. [Method] Field surveys were conducted in Shayang, Dangyang, Zhijiang, Yidu and Changyang of Hubei Province during 2009- 2011. Clubfoot disease of rapeseed samples from the surveyed locations were con- firmed by PCR detection and plants infection experiment. The factors of yield and plot yields influenced by clubroot disease were determined in plot experiment. [Re- salt] Rapeseed clubroots were confirmed in Zhijiang and Dangyang and not found in Shayang and Yidu. Clubroot was found in cruciferous vegetables but not in rapeseed d in Changyang. Infection of P. brassicae significantly decreased of the first effective branch numbers of rapeseed, effective pod number per plant, seed number per pod and 1 000-grain weight. Yield of infected rapeseed decreased by 56.4% over non-infected control. [Conclusion] Rapeseed clubroot disease mainly distributed in Zhijiang and Dangyang of Hubei Province. The disease had an economic impact on rapeseed production.展开更多
The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (la...The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.展开更多
Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological...Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of tire lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the First mitotic figures all appeared in the cortical cells about I mm front the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip, However, the adjacent cells partially synchronously proceeded their cell cycle.展开更多
[Objective] The aim was to study on characteristics of retoon rice yield components, and relationship of the characteristics with born bud rate and root ac- tivity of retoon rice before harvesting. [Method] Eighteen m...[Objective] The aim was to study on characteristics of retoon rice yield components, and relationship of the characteristics with born bud rate and root ac- tivity of retoon rice before harvesting. [Method] Eighteen mid-season hybrid rice va- rieties approved recently were taken as materials in high-yield cultivation conditions. Characteristics of retoon rice yield components were analyzed, and relationship of yield components characteristics with the rate of bud emergence and root activity of retoon rice before harvesting was explored, based on related data. [Result] Highly- yielding retoon rice is characterized by high effective ear number （Xl） and high set- ting percentage （X4）; the number of germinated shoots the 5^＊ d after first-batch rice was cut is a key factor for retoon rice yield and was of positive correlation with re- toon rice seedlings and effective ears, with correlation coefficients at 0.80^＊＊ and 0.76^＊＊. In addition, bleeding sap was of positive correlation with grain number and setting percentage of retoon rice and correlation coefficients were 0.50^＊ and 0.48^＊＊, and of positive correlation with average panicle weight, with correlation coefficient at 0.63. [Conclusion] It is an effective way for the rate of bud emergence and root activity at lar ecological zones. mproving retoon rice yield to increase harvesting in the test sites and simi lar ecological zones.展开更多
基金Supported by the Industry Technology System of Rapeseed in China(nycytx-005)Special Fund for Agro-scientific Research in the Public Interest(201003029)National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2010BAD01B04)~~
文摘[Objective] The aim of this study was to reveal the distribution of rapeseed clubfoot disease in Hubei Province and to assess the yield loss caused by the pathogen, Plasmodiophora brassicae. [Method] Field surveys were conducted in Shayang, Dangyang, Zhijiang, Yidu and Changyang of Hubei Province during 2009- 2011. Clubfoot disease of rapeseed samples from the surveyed locations were con- firmed by PCR detection and plants infection experiment. The factors of yield and plot yields influenced by clubroot disease were determined in plot experiment. [Re- salt] Rapeseed clubroots were confirmed in Zhijiang and Dangyang and not found in Shayang and Yidu. Clubroot was found in cruciferous vegetables but not in rapeseed d in Changyang. Infection of P. brassicae significantly decreased of the first effective branch numbers of rapeseed, effective pod number per plant, seed number per pod and 1 000-grain weight. Yield of infected rapeseed decreased by 56.4% over non-infected control. [Conclusion] Rapeseed clubroot disease mainly distributed in Zhijiang and Dangyang of Hubei Province. The disease had an economic impact on rapeseed production.
文摘The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.
文摘Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of tire lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the First mitotic figures all appeared in the cortical cells about I mm front the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip, However, the adjacent cells partially synchronously proceeded their cell cycle.
基金Supported by National Rice Industry Technology SystemNational Bumper-Crop Science and Technology Project(2011BAD16B05)~~
文摘[Objective] The aim was to study on characteristics of retoon rice yield components, and relationship of the characteristics with born bud rate and root ac- tivity of retoon rice before harvesting. [Method] Eighteen mid-season hybrid rice va- rieties approved recently were taken as materials in high-yield cultivation conditions. Characteristics of retoon rice yield components were analyzed, and relationship of yield components characteristics with the rate of bud emergence and root activity of retoon rice before harvesting was explored, based on related data. [Result] Highly- yielding retoon rice is characterized by high effective ear number （Xl） and high set- ting percentage （X4）; the number of germinated shoots the 5^＊ d after first-batch rice was cut is a key factor for retoon rice yield and was of positive correlation with re- toon rice seedlings and effective ears, with correlation coefficients at 0.80^＊＊ and 0.76^＊＊. In addition, bleeding sap was of positive correlation with grain number and setting percentage of retoon rice and correlation coefficients were 0.50^＊ and 0.48^＊＊, and of positive correlation with average panicle weight, with correlation coefficient at 0.63. [Conclusion] It is an effective way for the rate of bud emergence and root activity at lar ecological zones. mproving retoon rice yield to increase harvesting in the test sites and simi lar ecological zones.
