濒危植物四合木(Tetraena mongolica Maxim)的离体培养组织学观察及再生植株的研究

本文以四合木(Tetraena monggolica Maxim)种子萌发12d的无菌苗为外植体进行离体培养,通过在MS+2,4—D 0.5mg/l+6-BA 0.1mg/L培养基上获得愈伤组织产生。然后对MS+2,4—D 0.1mg/L+6-BA0.25mg/L+500mg/L水解酪蛋白培养基上获得胚状体及试管苗并进行了组织学观察,结果表明:胚状体来源于多种发育形式,它由单细胞、愈伤组织经不均等或均等分裂形成的细胞后代继续发育为各级胚至试管苗。

Comparative Study of Rice Morphogenesis with Different Cultivation Methods

[Objective] The aim of this study was to compare the morphological char- acteristics of rice （Oryza sativa L.） with different cultivation methods and investigate the dynamics of organ growth and development characteristics of different rice culti- vars. [Method] Based on continuous field observation and destructive sampling over a growing season, detailed organ morphological data were obtained including leaf length, node number, plant height, tiller number, leaf angle, leaf area and specific leaf weight, to compare organ morphological differences among 4 rice cultivars of Baidao （indica）, Jinnanfeng （japonica）, 9325 （japonica） and 9915 （japonica） with 3 cultivation methods of field planting in Weigang, pot planting in Weigang, and field planting in Jiangpu. [Result] Maximum leaf length of each node gradually increased at the early growth stage and decreased at the later growth stage, the relationship between maximum leaf length and node position can be described by the equations y=a,,-~ and y=ax＋b; node number, growth duration, leaf length and plant height of pot planting rice in Weigang were smaller than that of the other two field planting meth- ods; the relationship between plant height and sunshine duration, plant height and GDD （growing degree days） can be described by the equation y=ax＋b, 19.23 ℃.d of GDD （≥10 ≥-d） and 8.12 h of sunshine duration were required to increase 1 cm of plant height; plant height, tiller number, and leaf area of Baidao were higher than that of the other 3 laponica rice cultivars, but the specific leaf weight and leaf angle were smaller. [Conclusion] Comparison of morphological characteristic differ- ences among rice cultivars is an important way to select water-saving and drought- tolerant rice varieties. In this study, the experimental results can be integrated into a rice functional-architectural model to simulate rice organ growth dynamics in a three- dimensional space, thereby providing reference for selecting water-saving and drought-tolerant rice cultivars.

Morphology and ontogeny of dendritic cells in rats at different development periods

AIM： To study the morphology and ontogeny of dendritic cells of Peyer＇s patches in rats at different development periods. METHODS： The morphometric and flow cytometric analyses were performed to detect all the parameters of villous-crypts axis and the number of OX62＋DC, OX62＋CD4＋SIRP＋DC, and OX62＋CD4-SIRP-DC in the small intestine in different groups of rats. The relationship between the parameters of villous-axis and the number of DC and DC subtype were analyzed. RESULTS： All morphometric parameters changed significantly with the development of pups in the different age groups （F = 10.751, 12.374, 16.527, 5.291, 3.486; P = 0.000, 0.000, 0.000, 0.001, 0.015）. Villous height levels were unstable and increased from 115.24μm to 140.43 μm as early as 3 wk postpartum. Villous area increased significantly between 5 and 7 wk postpartum, peeked up to 13817.60 tam2 at 7 wk postpartum. Villous height and crypt depth ratios were relatively stable and increased significantly from 2.80 ＋ 1.01 to 4.54 =1= 1.56, 9-11 wk postpartum. The expression of OX62＋DC increased from 33.30%±5.80% to 80%± 17.30%, 3-11 wk postpartum （F =5.536, P = 0.0013）. OX62＋CD4＋SIRP＋DC subset levels detected in single-cell suspensions of rat total Peyer＇s patch dendritic cells （PP-DCs） increased significantly from 30.73% ± 5.16% to 35.50% ± 4.08%, 5-7 wk postpartum and from 34.20% ±1.35% to 43.60% ± 2.07% 9-11 wk postpartum （F = 7.216, P = 0.005）. CONCLUSION： This study confirms the agerelated changes in villous-crypt axis differentiation in the small intestine. Simultaneously, there are also development and maturation in rat PP-DCs phenotypic expression. Furthermore, the morphological changes of intestinal mucosa and the development of immune cells （especially DC） peaked at 9-11 wk postpartum, indicating that the intestinal mucosae reached a relatively mature state at 11 wk postpartum.

Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas

Increasing evidence indicates that transforming growth factor β （TGF-β） signaling pathways play many important roles in the early development of mollusks. However, limited information is known concerning their detailed mechanisms. Here, we describe the identification, cloning and characterization of two Smad genes, the key components of TGF-β signaling pathways, from the Pacific oyster Crassostrea gigas. Sequence analysis of the two genes, designated as cgi-smad1/5/8 and cgi-smad4, revealed conserved functional characteristics. The two genes were widely expressed in embryos and larvae, suggesting multiple roles in the early development of C. gigas. The mRNA of the two genes aggregated in the D quadrant and cgi-smad4 was highly expressed on the dorsal side of the gastrula, indicating that TGF-β signaling pathways may be involved in dorsoventral patterning in C. gigas. Furthermore, high expression levels of the two genes in the shell fields of embryos at different stages suggested important roles for TGF-β signaling pathways in particular phases of shell development, including the formation of the initial shell field and the biomineralization of larval shells. The results of this study provide fundamental support for elucidating how TGF-β signaling pathways participate in the early development of bivalve mollusks, and suggest that further work is warranted to this end.

Structure,expression,and developmental function of early divergent forms of metalloproteinases in Hydra

Metalloproteinases have a critical role in a broad spectrum of cellular processesranging from the break-down of extracellular matrix to the processing of signaltransduction-related proteins.These hydrolytic functions underlie a variety of mechanisms related to developmental processes as well as disease states.Structural analysis of metalloproteinases from both invertebrate and vertebrate species indicates that these enzymes are highly conserved and arose early during metazoan evolution.In this regard,studies from vari-ous laboratories have reported that a number of classes of metalloproteinases are found in hydra,a member of Cnidaria,the second oldest of existing animal phyla.These studies demonstrate that the hydra genome contains at least three classes of metalloproteinases to include members of the 1)astacin class,2)matrix met-alloproteinase class,and 3)neprilysin class.Functional studies indicate that these metalloproteinases play diverse and important roles in hydra morphogenesis and cell differentiation as well as specialized functions in adult polyps.This article will review the structure,expression,and function of these metalloproteinases in hydra.