遵义师范学院图书馆1985-2013年发表论文分析

运用文献计量分析方法对遵义师范学院图书馆1985-2013年在期刊上发表的论文,分别从期刊类型分布、核心期刊分布、高产作者分布、高频被引论文分布、高频被引作者分布和学者学术水平值分布进行了统计分析,从而揭示了遵义师范学院图书馆近30年学术研究的发展进程。

遵义市图书馆专业人员发表论文分析

本文对遵义市图书馆1994～2013年在期刊和会议论文集上发表的论文，分别从论文发表的年代分布、论文类型分布、期刊类型分布、论文篇数及作者人数分布和高产作者分布进行统计分析，从而揭示了遵义市图书馆20年学术研究的发展进程。

我国16个省会城市公共图书馆(2015—2019)发文分析

运用文献计量分析方法,对我国16个省会城市公共图书馆(2015—2019)年在学术期刊上发表的论文,分别从论文年代分布、期刊类型分布、图书情报学核心期刊分布、高产作者分布、高频被引论文分布和学术研究团体实力分布进行了统计分析,从而揭示了我国16个省会城市公共图书馆学术研究的发展进程。

基于世界顶级学术期刊的中国酒店研究概述

本文对1978年．2012年间发表在三大权威酒店期刊《Cornell Hospitality Quarterly》（CHQ）,《International Journal of Hospitality Management》（IJHM）以及《Journal of Hospitality ＆ Tourism Research》（JHTR）上的有关中国酒店研究的80篇文献进行了综述，以分析和展现国际上中国酒店研究的变革和发展。分析发现，中国酒店研究在近5年（2008-2012）的发展尤为迅猛，研究视角、研究方法、数据收集方式及分析方法都日趋多样和科学。在地区贡献上，以亚太地区为主，未来亟需更多跨地区的合作。但是，本文的文献回顾范围仍有待扩充，今后的综述研究可以尝试运用更多的分析标准以及标准之间的交叉制表分析。

某疾控中心职工科技论文发表情况分析

医学论文是医学科研成果的主要体现方式,论文数量和质量反映了一个单位的科研状况和科研人员的学术水平,是评价单位科研工作的重要指标,通过发表论文的分析有助于了解各学科建设发展的状况和学术水平,探索业务技术发展的规律。本文对浙江省疾病预防控制中心2008—2009年发表的论文进行统计分析,为学术发展和学科研究提供参考。

我国“十一五”期间公共图书馆发表论文分析

运用文献计量分析方法对我国公共图书馆机构2006 2010年在图书馆学10种核心期刊上发表的论文,分别从期刊分布、地区分布、高发文机构分布、作者发文人数及篇数分布、高产作者分布、高频被引论文分布和高频被引作者分布进行了统计分析,从而揭示了我国"十一五"期间公共图书馆学术研究的发展进程。

Expression and Phylogenetic Analysis of Pea Actin Isoforms

Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence in the untranslated regions. RT_PCR and Southern blotting using 3′_untranslated region (3′_UTR) as specific probe revealed that pea isoactin genes were expressed in roots, stems, leaves, tendrils, pollen and juvenile siliques, but displayed different patterns of transcript accumulation. Two_fold serial dilution electrophoresis showed PEAcⅠ mRNA preferentially accumulated in rapidly developing tissues: it peaked in seven days' stem; remained at a rather high level in leaves within a month but decreased significantly later; varied a little in tendrils and reached a median and a very low level respectively in juvenile siliques and in pollen. PEAcⅡ displayed somewhat similar expression pattern to PEAcⅠ. The observed differences in sequences and transcript accumulation patterns suggest that the individual pea actin genes may differ in their transcriptional regulation and cellular function. Phylogenetic tree of actins showed that pea actin isoforms are as diverged from each other as they are from other plant actins, and pea actins might have originated from a common ancestor before the divergence of the dicot and monocot plants.

我国15个副省级公共图书馆2010年至2013年发表论文分析

运用文献计量分析方法对我国15个副省级公共图书馆(2010-2013)年在期刊、会议论文集和辑刊上发表的论文,分别从论文类型分布、期刊类型分布、图书情报学核心期刊分布、高产作者分布、高频被引论文分布、高频被引作者分布和机构发文、被引情况及作者人数分布进行了统计分析,从而揭示了我国15个副省级公共图书馆学术研究的发展进程。

某医院2010-2013年科技论文发表情况分析

我院是一所集医疗、预防、保健、康复、教学及科研为一体的大型综合性医院,而科研论文则是医院科研成果的重要表现形式,也是体现医院在科研能力方面的重要指标,一个单位的科研论文产出数量和质量从一个侧面反映了其科研实力和科技发展水平。本文通过文献计量统计学方法,通过对我院2010—2013年发表在国内外期刊的科技论文进行统计分析,旨在发现医院的科研水平以及存在的不足,为今后医院在科研管理和学术水平的提升提供科学的参考依据。

Salt-Water Dynamics in Soils: II. Effect of Precipitation on Salt-Water Dynamics

Through a simulation test carried out with soil columns (61.8 cm in diameter), the effect of precipitation on salt-water dynamics in soils was studied by in-situ monitoring of salt-water dynamics using soil salinity sensors and tensiometers. The results show that in the profile of whole silty loam soil, the surface runoff volume due to precipitation and the salt-leaching role of infiltrated precipitation increased with the depth of ground water; and in the profile with an intercalated bed of clay or with a thick upper layer of clay, the amount of surface runoff was greater but the salt-leaching role of precipitation was smaller than those in the profile of whole silty loam soil. In case of soil water being supplemented by precipitation, the evaporation of groundwater in the soil columns reduced, resulting in a great decline of salt accumulation from soil profile to surface soil. The effect of precipitation on the water regime of soil profile was performed via both water infiltration and water pressure transfer. The direct infiltration depth of precipitation was less than 1 m in general, but water pressure transfer could go up to groundwater surface directly.

Phylogeny of Saccharina and Laminaria(Laminariaceae, Laminariales, Phaeophyta) in sequence-tagged-site markers

Laminaria and Saccharina have recently been recognized as two independent clades from the former genus Laminaria. Traditional morphological taxonomy is being challenged by molecular evidence from both nucleus and plastid. Intensive work is in great demand from the perspective of genome colinearity. In this study, 118 sequence-tagged site(STS) markers were screened for phylogenetic analyses, 29 based on genome sequences, while 89 were based on expressed sequence tag(EST) sequences. EST-based STS marker development(29.37%) had an effi ciency twice as high as genome-sequence-based development(9.48%) as a result of high conservation of gene transcripts among the relative species. S. ochotensis, S. religiosa, S. japonica, and L. hyperborea showed great homogeneity in all 118 STS markers. Our result supports the view that the diversifi cation between the genera Saccharina and Laminaria was a more recent event and that Saccharina and Laminaria shared high phylogenetic affi nity. However, when it came to the single nucleotide polymorphism(SNP) level among the 41 SNPs, L. hyperborea owned 29 unique SNPs against 12 within the left three Saccharina species and 12 of the 13 indels were supposedly unique for L. hyperborea, indicated by its high variability. Originating from homologous ancestors, species between the recently diverged genera Laminaria and Saccharina may have taken in enough mutations at the SNP level only, in spite of different evolutionary strategies for better adaptation to the environment. Our study lays a solid foundation from a new perspective, although more accurate phylogenetic analysis is still needed to clarify the evolutionary traces between the genera Saccharina and Laminaria.

Expression Analysis of the Insulin-Like Growth Factors Ⅰ and Ⅱ During Embryonic and Early Larval Development of Turbot(Scophthalmus maximus)

The insulin-like growth factors Ⅰ and Ⅱ （IGF-Ⅰ and IGF-Ⅱ） are important proteins involved in fish growth and develop- ment. Here, we report the isolation of IGF-Ⅱ and expression analysis of IGFs in turbot Scophthalmus maximus, aiming to clarify their function in embryonic and larval development of fish. The deduced IGF-Ⅱ gene is 808 bp in full length, which encodes a protein of 219 amino acids and is 93% similar with that ofParalichthys olicaceus in amino acid sequence. The tissue abundance and the ex- pression pattern of IGFs in a turbot at early development stages were investigated via reverse transcription-polymer chain reaction. Result showed that the IGF-Ⅰ and IGF-Ⅱ genes were widely expressed in tissues of S. maximus. IGF-Ⅰ was detected in all tissues ex- cept intestines with the highest level in liver, while IGF-Ⅱ transcript presented in all tissues except muscle. At the stages of embry- onic and larval development, the mRNA levels of IGFs sharply increased from the stage of unfertilized egg to post larva, followed by a decrease with larval development. However, there was an increase in IGF-Ⅰ at the embryonic stage and IGF-Ⅱ at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. Our study provides reference data for further investigation of growth regulation in turbot, which can guarantee better understanding of the physiological role that IGFs play in fish.

Characterization of Pigeon Paramyxoviruses (Newcastle disease virus) Isolated in Kazakhstan in 2005

Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the amplified F protein DNA product (nucleotide sequence 47-422) and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 (PPMV-1) isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116* F117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.

递归累积Meta分析简介

Meta分析包括已发表文献的Meta分析(meta-analysis of the published literature,MPL)和单个病例资料的Meta分析(meta-analysis of individual patient data,MIPD)。递归累积Meta分析是一种可对已有资料重新整理并及时更新,还能对现有试验的延续随访进行分析的Meta分析方法,递归累积Meta分析在每纳入一项新研究或纳入更新的研究时,可以检测每一合并步骤中效应量的波动,从而判断纳入研究间是否存在偏倚或异质性,并判断合并结果的稳定性。本文主要介绍了递归累积Meta分析的概念并结合具体实例来讲解如何实现。

加大改革力度 加快转变步伐 推进全省供销社工业再上新台阶

在今年河北省供销社科技工业工作会议上，刘伯芳主任、黄思厚副主任，火力推进两个转变。

Identification and expression of amphioxus AmphiSmad1/5/8 and AmphiSmad4

Smad family proteins are identified as intracellular signal mediators of the TGF-β superfamily.In this study,we identified two novel members of the Smad family,termed as AmphiSmad1/5/8 and AmphiSmad4,from Chinese amphioxus.Both AmphiSmad1/5/8 and AmphiSmad4 showed a typical domain structure of Smad proteins consisting of conserved MH1 and MH2 domains.Phylogenetic analysis placed AmphiSmad1/5/8 in the Smad1,5 and 8 subgroup of the R-Smad subfamily,and AmphiSmad4 in the Co-Smad subfamily.The spatial and temporal gene expression patterns of AmphiSmad1/5/8 and AmphiSmad4 showed that they may be involved in the embryonic development of notochord,myotome and alimentary canal,and may help to establish the specification of dorsal-ventral axis of amphioxus.Moreover,AmphiSmad1/5/8 and AmphiSmad4 showed extensive distribution in all adult tissues examined,suggesting that these two genes may play important roles in the morphogenesis of a variety of tissues especially notochord and gonad.

A microarray analysis of early activated pathways in concanavalin A-induced hepatitis

Objective:To explore the mechanisms of fulminant hepatitis(FH) in the early stages,and to determine the critical pathways in its initiation and progression.Methods:Twelve BALB/c mice were divided into four groups:one group left as negative control and sacrificed immediately after injection of phosphate-buffered saline(PBS),and another three groups with concanavalin A(Con A) administration sacrificed at 1,3,and 6 h after injection.Affymetrix GeneChip Mouse 430 2.0 Array was employed to evaluate the expression profile of each of the 12 samples.Further analysis was done on the microarray data to extract the genes that were differentially expressed.Enrichment analysis was carried out to determine relevant pathways within which regulated genes were significantly enriched.Results:A total of 393,8354 and 11 344 differentially expressed genes were found,respectively,at three time points.During 0-1 h and 1-3 h,most of the pathways enriched with regulated genes were related to immune response and inflammation,among which Toll-like receptor(TLR) signaling and mitogen-activated protein kinase(MAPK) signaling appeared during both phases,while cytokine-cytokine receptor interaction,apoptosis,T cell receptor signaling,and natural killer(NK) cell-mediated cytotoxicity pathways emerged during the second phase.Pathways found to be significant during 3-6 h were mostly related to metabolic processes.Conclusion:The TLR signaling pathway dominates the early responses of Con A-induced FH in mice.It stimulates the production of type I cytokines,therefore recruiting and activating T/NK cells.Activated T/NK cells exert their cytotoxicity on hepatocytes through inducing death receptorintermediated apoptosis,resulting in liver injury.

Isolation and characterization of a Klebsiella pneumoniae strain from mangrove sediment for efficient biosynthesis of 1,3-propanediol

1,3-Propanediol （PDO） is an important bulk industrial material. It can be produced by microbial fermentation. In this study, the microbial flora of mangrove sediment was screened to identify strains with high production of PDO by fermentation of glycerol. The PDO productivities of the isolated strains were tested, and the strain with highest PDO productivity was characterized using the API20E and 16-s rRNA sequence analysis. The physiological and phylogenetic analysis indicated that the strain was closed related to K. pneumoniae species and was named as K. pneumoniae HSL4. The structure of the dha cluster which was responsible for the biosynthesis of PDO was analyzed. It is observed that K. pneumoniae HSL4 was tolerant to salt and partly tolerant to acetate and lactate, which will favor industrial applications. Fed-batch fermentation experiments revealed K. pneumoniae HSL4 exhibited an excellent ability to produce PDO with high concentration （80.08 g L^-1）, productivity （2.22 g L^-1h^-1） and conversion （0.435 g g^-1 or 0.53 mol mol^-1）. The metabolic flux profile illuminated that glycerol was consumed rapidly and PDO was accumulated quickly to a high level during the exponential growth phase. This study provided important information for further fermentation and metabolic engineering of PDO production by K. pneumoniae HSL4.