To investigate the characteristics of hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL), batch experiments are conducted under different conditions. Hydrogen produc...To investigate the characteristics of hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL), batch experiments are conducted under different conditions. Hydrogen production has a correlation with cell growth and the consumption of glucose and soluble protein. The optimum pH for cell growth is 4.5±0.15. At acidic pH 4.0±0.15, the bacteria has the maximum accumulated hydrogen volume of 2382 ml/L culture and the maximum hydrogen evolution rate of 339.9 ml/L culture·h with 1% glucose. The optimum temperature for cell growth and hydrogen production is 35℃. In addition, fermentative hydrogen-producing bacterial strain B49 can generate hydrogen from the decomposition of other organic substrates such as wheat, soybean, corn, and potato. Moreover, it can also produce hydrogen from molasses wastewater and brewage wastewater, and hydrogen yields are 137.9 ml H 2/g COD and 49.9 ml H 2/g COD, respectively.展开更多
Controlling the biogas fermentation process is the key for maintaining stable operation of biogas system and increasing gas yield. Aiming at features of biogas fermentation process and difficulties of control, a pract...Controlling the biogas fermentation process is the key for maintaining stable operation of biogas system and increasing gas yield. Aiming at features of biogas fermentation process and difficulties of control, a practical control scheme is proposed combining the abundant experience of biogas experts. And it discussed the structural design and hardware configuration of the expert control system, established the database and role base, and designed the control strategy of production system inference. The design scheme with ZigBee and PDA technology as core is employed so as to solve problems of environmental factor detection and data transfer management. The test result shows that the deviation of temperature is controlled within ± 0.9℃, the deviation of pH is controlled within ±0.3, the deviation of oxidation-reduction potential is controlled within ±30mV, the deviation of gas production is controlled within ± 9mL and that of methane concentration is controlled within ±4.5%. This system is easily expandable and applicable to biogas engineering at various scales.展开更多
文摘To investigate the characteristics of hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL), batch experiments are conducted under different conditions. Hydrogen production has a correlation with cell growth and the consumption of glucose and soluble protein. The optimum pH for cell growth is 4.5±0.15. At acidic pH 4.0±0.15, the bacteria has the maximum accumulated hydrogen volume of 2382 ml/L culture and the maximum hydrogen evolution rate of 339.9 ml/L culture·h with 1% glucose. The optimum temperature for cell growth and hydrogen production is 35℃. In addition, fermentative hydrogen-producing bacterial strain B49 can generate hydrogen from the decomposition of other organic substrates such as wheat, soybean, corn, and potato. Moreover, it can also produce hydrogen from molasses wastewater and brewage wastewater, and hydrogen yields are 137.9 ml H 2/g COD and 49.9 ml H 2/g COD, respectively.
文摘Controlling the biogas fermentation process is the key for maintaining stable operation of biogas system and increasing gas yield. Aiming at features of biogas fermentation process and difficulties of control, a practical control scheme is proposed combining the abundant experience of biogas experts. And it discussed the structural design and hardware configuration of the expert control system, established the database and role base, and designed the control strategy of production system inference. The design scheme with ZigBee and PDA technology as core is employed so as to solve problems of environmental factor detection and data transfer management. The test result shows that the deviation of temperature is controlled within ± 0.9℃, the deviation of pH is controlled within ±0.3, the deviation of oxidation-reduction potential is controlled within ±30mV, the deviation of gas production is controlled within ± 9mL and that of methane concentration is controlled within ±4.5%. This system is easily expandable and applicable to biogas engineering at various scales.
