Rhizobium leguminosarum\ L003,a kind of biofertilizers,was cultured in a periodic air pressure oscillating,solid\|state fermenter by using straw of wheat as an inert solid support.Effects of air pressure oscillation a...Rhizobium leguminosarum\ L003,a kind of biofertilizers,was cultured in a periodic air pressure oscillating,solid\|state fermenter by using straw of wheat as an inert solid support.Effects of air pressure oscillation amplitude,frequency on the viable cells of \R.leguminosarum\ L003 and oxygen transfer rate were investigated.It was found that enhanced oxygen transfer and biochemical reaction occurred in this system.Under the optimized conditions,about a 3\|fold increase of the viable cells was obtained in this system compared with that in a static tray fermenter.The overall oxygen transfer rate reached 487.01 mmol/(kg\5h)in this system in case of 0.35 MPa, t \-2=0.5 min against 37.46 mmol/(kg\5h) in static tray fermenter.展开更多
L-threonine(L-Thr) obtained by fermentation often contains vestigial hydrosoluble Pb(Ⅱ), Fe(Ⅱ), L-glutamic acid(L-Glu) etc., which affect the product quality. Poly melamine and L-aspartic acid(L-Asp) resin functiona...L-threonine(L-Thr) obtained by fermentation often contains vestigial hydrosoluble Pb(Ⅱ), Fe(Ⅱ), L-glutamic acid(L-Glu) etc., which affect the product quality. Poly melamine and L-aspartic acid(L-Asp) resin functional coconut shell activated carbon composite(PMA/AC) was prepared by a pressure relief-dipping-microwave assisted polymerization method for the simultaneous removals. The adsorption capacities of Pb(Ⅱ), Fe(Ⅱ) and L-Glu could reach to 82.34 mg·g^(-1), 57.82 mg·g^(-1) and 102.58 mg·g^(-1) at conditions of pH 5.0, 45 °C and 4 h with an initial concentration of 0.01 mol·L^(-1), respectively. The present PMA/AC was successfully used to the simultaneous removals of vestigial Pb(Ⅱ), Fe(Ⅱ) and L-Glu from the fermented crude product solution of L-Thr. Moreover, the PMA/AC was carefully characterized by FE-SEM, IR et al. analysis techniques, the results show that abundant PMA particles evenly distributed at the inner and outside surface of AC with a size of(50 ± 20) nm.展开更多
文摘Rhizobium leguminosarum\ L003,a kind of biofertilizers,was cultured in a periodic air pressure oscillating,solid\|state fermenter by using straw of wheat as an inert solid support.Effects of air pressure oscillation amplitude,frequency on the viable cells of \R.leguminosarum\ L003 and oxygen transfer rate were investigated.It was found that enhanced oxygen transfer and biochemical reaction occurred in this system.Under the optimized conditions,about a 3\|fold increase of the viable cells was obtained in this system compared with that in a static tray fermenter.The overall oxygen transfer rate reached 487.01 mmol/(kg\5h)in this system in case of 0.35 MPa, t \-2=0.5 min against 37.46 mmol/(kg\5h) in static tray fermenter.
基金Supported by the National Natural Science Foundation of China(U1407110)2014 Special Funds of Guangdong Province for Collaborative Innovation and Environment Platform Construction,Public Research and Capacity Building(2014B090901010)
文摘L-threonine(L-Thr) obtained by fermentation often contains vestigial hydrosoluble Pb(Ⅱ), Fe(Ⅱ), L-glutamic acid(L-Glu) etc., which affect the product quality. Poly melamine and L-aspartic acid(L-Asp) resin functional coconut shell activated carbon composite(PMA/AC) was prepared by a pressure relief-dipping-microwave assisted polymerization method for the simultaneous removals. The adsorption capacities of Pb(Ⅱ), Fe(Ⅱ) and L-Glu could reach to 82.34 mg·g^(-1), 57.82 mg·g^(-1) and 102.58 mg·g^(-1) at conditions of pH 5.0, 45 °C and 4 h with an initial concentration of 0.01 mol·L^(-1), respectively. The present PMA/AC was successfully used to the simultaneous removals of vestigial Pb(Ⅱ), Fe(Ⅱ) and L-Glu from the fermented crude product solution of L-Thr. Moreover, the PMA/AC was carefully characterized by FE-SEM, IR et al. analysis techniques, the results show that abundant PMA particles evenly distributed at the inner and outside surface of AC with a size of(50 ± 20) nm.
