目的分离和鉴定引起小米乳酸菌发酵饮料胀罐的微生物,并对所分离菌株的产气情况进行研究。方法使用孟加拉红琼脂培养基、营养琼脂培养基和改良MRS琼脂培养基对引起小米乳酸菌发酵饮料胀罐的微生物进行分离和纯化,根据菌落形态观察、菌...目的分离和鉴定引起小米乳酸菌发酵饮料胀罐的微生物,并对所分离菌株的产气情况进行研究。方法使用孟加拉红琼脂培养基、营养琼脂培养基和改良MRS琼脂培养基对引起小米乳酸菌发酵饮料胀罐的微生物进行分离和纯化,根据菌落形态观察、菌体形态观察、16S rRNA序列分析并结合基质辅助激光解析/电离飞行时间质谱法(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)对分离菌株进行鉴定。最后将分离菌株接种到灭菌的小米乳酸菌饮料产品中,培养观察产气现象。结果共分离出6株细菌,编号为B001、B002、B003、B004、B005、B006,均鉴定为副干酪乳杆菌(Lactobacillus paracasei),6株细菌均具有产气现象。结论副干酪乳杆菌是引起本研究产品胀罐的主要原因。展开更多
This research was carried out to study the effect of fermentation on the chemical composition, anti-nutrient content, PH, titratable acidity, and microbiological changes of millet and soyabean blend. Millet and soyabe...This research was carried out to study the effect of fermentation on the chemical composition, anti-nutrient content, PH, titratable acidity, and microbiological changes of millet and soyabean blend. Millet and soyabean composite flours were mixed in gram of six combinations as follows millet and soyabean (A) = 100:0, millet and soyabean (B) = 90:10, millet and soyabean (C) = 80:20, millet and soyabean (D) = 70:30, millet and soyabean (E) = 60:40, millet and soyabean (F) = 50:50 and subjected to natural fermentation for 72 h. The following bacteria isolates were obtained from the fermentation; Lacobacillus fermentum, L. acidophilus, L. bulgaricus, L. plantarum, L. dextranicum, L. rhamnosus, L. delbrueckii, L. leichemanii, L. divergens, L. reuteri, L. jenseni, L. casei, L. salivarius, L. cellubiosus, Leuconostoc mesenteroide and Pediococcus acidilactis, of which L. plantarum was the most dominant the throughout the period of fermentation. There was decrease in pH with increase in TTA in all the samples. The result of the proximate analysis revealed a marginal increase in crude protein content of each sample (from 44.41 to 63.14, from 11.02 to 24.02, from 16.64 to 23.10, from 20.83 to 26.93, from 25.43 to 30.12, 39.12 to 35.86 and from 40.66 to 54.24%) There was increase in ash content and decrease in carbohydrate, fibre and fat contents of the fermented samples. Results from this research also show significant reduction in anti-nutritional content which are hydrogen cyanide, oxalate and phytate. Fermentation has modified the microbial and nutritional quality of the millet and soyabean blend and this has greatly improved the nutrient content of the blend.展开更多
文摘目的分离和鉴定引起小米乳酸菌发酵饮料胀罐的微生物,并对所分离菌株的产气情况进行研究。方法使用孟加拉红琼脂培养基、营养琼脂培养基和改良MRS琼脂培养基对引起小米乳酸菌发酵饮料胀罐的微生物进行分离和纯化,根据菌落形态观察、菌体形态观察、16S rRNA序列分析并结合基质辅助激光解析/电离飞行时间质谱法(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)对分离菌株进行鉴定。最后将分离菌株接种到灭菌的小米乳酸菌饮料产品中,培养观察产气现象。结果共分离出6株细菌,编号为B001、B002、B003、B004、B005、B006,均鉴定为副干酪乳杆菌(Lactobacillus paracasei),6株细菌均具有产气现象。结论副干酪乳杆菌是引起本研究产品胀罐的主要原因。
文摘This research was carried out to study the effect of fermentation on the chemical composition, anti-nutrient content, PH, titratable acidity, and microbiological changes of millet and soyabean blend. Millet and soyabean composite flours were mixed in gram of six combinations as follows millet and soyabean (A) = 100:0, millet and soyabean (B) = 90:10, millet and soyabean (C) = 80:20, millet and soyabean (D) = 70:30, millet and soyabean (E) = 60:40, millet and soyabean (F) = 50:50 and subjected to natural fermentation for 72 h. The following bacteria isolates were obtained from the fermentation; Lacobacillus fermentum, L. acidophilus, L. bulgaricus, L. plantarum, L. dextranicum, L. rhamnosus, L. delbrueckii, L. leichemanii, L. divergens, L. reuteri, L. jenseni, L. casei, L. salivarius, L. cellubiosus, Leuconostoc mesenteroide and Pediococcus acidilactis, of which L. plantarum was the most dominant the throughout the period of fermentation. There was decrease in pH with increase in TTA in all the samples. The result of the proximate analysis revealed a marginal increase in crude protein content of each sample (from 44.41 to 63.14, from 11.02 to 24.02, from 16.64 to 23.10, from 20.83 to 26.93, from 25.43 to 30.12, 39.12 to 35.86 and from 40.66 to 54.24%) There was increase in ash content and decrease in carbohydrate, fibre and fat contents of the fermented samples. Results from this research also show significant reduction in anti-nutritional content which are hydrogen cyanide, oxalate and phytate. Fermentation has modified the microbial and nutritional quality of the millet and soyabean blend and this has greatly improved the nutrient content of the blend.