金耳(Tremella mesenterica Retz ex Fr)又名黄木耳,为银耳科、银耳属真菌,性味甘、平,它是一种营养价值较高的真菌类。被视为“补益身心,延年益寿”的珍品。过去曾有少量报道金耳具有抗癌、保肝、抗衰老等药理作用,金耳糖酞有增强免疫...金耳(Tremella mesenterica Retz ex Fr)又名黄木耳,为银耳科、银耳属真菌,性味甘、平,它是一种营养价值较高的真菌类。被视为“补益身心,延年益寿”的珍品。过去曾有少量报道金耳具有抗癌、保肝、抗衰老等药理作用,金耳糖酞有增强免疫功能的作用。用于本项研究的金耳发酵液多糖是采用人工深层发酵方法,获得菌丝和滤液,金耳发酵液多糖从滤液中提取获得。展开更多
Strain of Ganoderma japonicum with higher mycelial growth rate and higher levels of polysaccharide production was selected from seven tested strains of Ganoderma. The effects of nitrogen source, carbon source and meta...Strain of Ganoderma japonicum with higher mycelial growth rate and higher levels of polysaccharide production was selected from seven tested strains of Ganoderma. The effects of nitrogen source, carbon source and metal ions on the polysaccharide production of selected Ganoderma japonicum were studied. The results showed that the optimum nitrogen source and carbon source were 2% and 0.2% ,respectively. And the existence of 0.2% Fe^2+ could improve the polysaccharide yield significantly. The result of fermentor enlargement test indicated that extracellular crude polysaccharide content per 100 mL fermentation liquor and mycelium content yielded highest amount of 181.7 mg and 151.0 mg,respectively,with relatively mild change of pH value. It was concluded that fermentation in fermentor was more suitable for the secretion of C, anoderma polysaccharide.展开更多
文摘金耳(Tremella mesenterica Retz ex Fr)又名黄木耳,为银耳科、银耳属真菌,性味甘、平,它是一种营养价值较高的真菌类。被视为“补益身心,延年益寿”的珍品。过去曾有少量报道金耳具有抗癌、保肝、抗衰老等药理作用,金耳糖酞有增强免疫功能的作用。用于本项研究的金耳发酵液多糖是采用人工深层发酵方法,获得菌丝和滤液,金耳发酵液多糖从滤液中提取获得。
文摘从姬松茸(Agaricus blazei)发酵液中分离纯化获得其主要成分多糖(AbEXP1-a),对其进行紫外扫描、元素分析和常规化理性分析,发现该多糖属于水溶性多糖,其中包含结合态蛋白,不与有机溶剂相溶,与乙醇、甲醇微溶;用HPLC法对多糖进行检测,得到多糖分子量,其保留时间为15.09 min,与标准曲线比较,得出其分子量为50 k D。用姬松茸发酵液多糖对小鼠进行了抗疲劳作用检测,其血红蛋白变化不突出;大负荷定量运动后,多糖组血尿氮含量为(7.84±1.45)mmoL·mL-1,血乳酸含量为(4.67±0.99)mmoL·mL-1,均比对照组低;多糖组力竭游泳时间(47.95±7.16)min,明显比对照组长。结果显示姬松茸胞内多糖具有较强的抗疲劳作用。
基金Supported by the Outstanding Foundation Program of Henan Province(074100510018)the Science and Technology Key Program of Henan Province(0624040003)~~
文摘Strain of Ganoderma japonicum with higher mycelial growth rate and higher levels of polysaccharide production was selected from seven tested strains of Ganoderma. The effects of nitrogen source, carbon source and metal ions on the polysaccharide production of selected Ganoderma japonicum were studied. The results showed that the optimum nitrogen source and carbon source were 2% and 0.2% ,respectively. And the existence of 0.2% Fe^2+ could improve the polysaccharide yield significantly. The result of fermentor enlargement test indicated that extracellular crude polysaccharide content per 100 mL fermentation liquor and mycelium content yielded highest amount of 181.7 mg and 151.0 mg,respectively,with relatively mild change of pH value. It was concluded that fermentation in fermentor was more suitable for the secretion of C, anoderma polysaccharide.