对我市无偿献血者职业分类调查分析

目的:寻找无偿献血宣传适宜的目标人群,采取有效方式,使本市无偿献血宣传工作能够有效开展。方法:选取本市2012年下半年参加献血者24 641例,对其进行年龄、性别、血型、职业等的分类统计。结果:自由职业者53.44%、工人12.11%、农民3.48%、教师6.36%、事业文员0.36%、军人0.32%、公司职员5.55%、医务人员5.06%、公务员7.17%、学生1.66%、其他4.49%,团体献血比例达到20%,而且献血者和年龄、文化程度、捐献次数、血型都具有一定的关系。结论:应针对团体企事业单位及城乡进行无偿献血宣传重点开发区域。

SNP Identification in α_(2A)-Adrenergic Receptor Gene in Chinese and the Effect on Gene Expression

Objective: To scan single nucleotide polymorphism ( SNP ) in Chinese alpha-2Aadrenergic receptor (α_(2A)-AR) gene and study the effects of the SNP on the gene expression.Methods: The complete sequence of α_(2A)-AR gene was analyzed with automated DNA sequencer to scanSNPs. Genomic DNA was extracted from whole blood and a 239 bp fragment containing the G/Cpolymorphism was amplified with PCR using a pair of. specific primers. PCR-RFLP was used to performthe genotyping of the SNP at the site-1 296 bp of the people in the North of China. Electrophoresismobility shift assay ( EMSA ) was used to study the binding of the 390 bp fragments (- 1 414-1 025bp) with G or C at the site-1 296 bp and nuclear extracts . Results: In our study, two SNPs werefound in α_(2A)-AR gene. Allele frequencies of the SNP at the site-1 296 bp were 0.61 and 0.39 forG and C , and the genotype frequencies were 0.34 , 0.54 and 0.13 for GG, GC and CC respectively fromthe people in the North of China. In the EMSA, a specific binding appeared in the complex ofnuclear extracts and DNA with C at-1 296 bp . Conclusion: Two SNPs exist in α_(2A)-AR gene from thepeople in the North of China , and DNA fragment with allele C of the SNP at the site-1 296 bp couldbind with a specific protein, which could influence the gene expression.