Objective： To explore the action mechanism of electroacupuncture （EA） in improving the gastric motility of rats with diabetic gastroparesis （DGP）. Methods： Forty-eight healthy Sprague-Dawley （SD） rats were ran...Objective： To explore the action mechanism of electroacupuncture （EA） in improving the gastric motility of rats with diabetic gastroparesis （DGP）. Methods： Forty-eight healthy Sprague-Dawley （SD） rats were randomly assigned into four groups： a normal group （group A）, a model group （group B）, a group of EA at acupoints （group C）, and a group of EA at non-acupoints （group D）, 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin （STZ） plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate （GER） were observed. The content of insulin （INS） in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay （ELISA）. The expression of growth hormone secretagogue receptor mRNA （GHSR mRNA） in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction （PCR）. Results：Compared with group A, blood glucose and urine glucose increased significantly （P〈0.01）, GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly （P〈0.05 orP〈0.01） in group B. Compared with group B, blood glucose and urine glucose decreased significantly （P〈0.05）, GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly （P〈0.05 orP〈0.01） in group C. Conclusion： EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.展开更多
Objective： To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor la （GHSR-la） in lateral septal nucleus of rats wi...Objective： To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor la （GHSR-la） in lateral septal nucleus of rats with diabetic 8astroparesis （DGP）, and to investigate the central regulatory mechanism of DGP treatment with point-moxibustion. Methods： Forty SPF-grade Sprague-Dawley （SD） rats were randomly divided into a blank group, a model group, an electroacupuncture （EA） group and a point-moxibustion group, with 10 rats in each group. A DGP rat model was established by intraperitoneal injection of 2% streptozotocin （STZ） with 8-week irregular high-sugar and high-fat diet in the model group, the EA group and the point-moxibustion group, and rats in the blank group were injected intraperitoneally with 0.1 mmoL/L （pH 4.5） citric acid-sodium citrate buffer with 8-week normal diet. Eight weeks later, rats in the EA group were treated by EA at Zusanli （ST 36）, Liangmen （ST 21） and Sanyinjiao （SP 6）; while rats in the point-moxibustion group were treated by point-moxibustion at Zusanli （ST 36）, Liangmen （ST 21） and Sanyinjiao （SP 6） for successive 15 d. Rats in the blank group and the model group were fixed as the control without intervention. After treatment, intestinal propulsion rate and gastric emptying rate were measured. The mRNA and protein expressions of ghrelin and GHSR-la in the lateral septal nucleus were detected by real-time polymerase chain reaction （RT-PCR） and Western blot （WB）. Results： Compared with the blank group, the intestinal propulsion rate and gastric emptying rate of the model group were significantly lower （both P〈0.01）; compared with the model group, the intestinal propulsion rate and gastric emptying rate of the EA group and the point-moxibustion group increased significantly （all P〈0.05）. The mRNA and protein expressions of ghrelin and GHSR-la were lower in the model group than those in the blank group （all P〈0.01）. The mRNA and protein expressions of ghrelin and GHSR-Ia were significantly higher in the EA group and the point-moxibustion group than those in the model group （all P〈0.05）. There were no statistically significant differences between the EA group and the point-moxibustion group （all P〉0.05）. Conclusion： Point-moxibustion at Zusanli （ST 36）, Liangmen （ST 21） and Sanyinjiao （SP 6） can increase the intestinal propulsion rate and gastric emptying rate of DGP rats, and promote the mRNA and protein expressions of ghrelin and GHSR-la in the central nervous system. The mechanism may be related to the activation of 8hrelin pathway in hypothalamic arcuate nucleus-lateral septal nucleus.展开更多
基金supported by National Natural Science Foundation of China No.81403487Youth Fund of Hunan Provincial Education Department No.14B128Open Fund Project of Hunan University Innovation Platform No.12K088~~
文摘Objective： To explore the action mechanism of electroacupuncture （EA） in improving the gastric motility of rats with diabetic gastroparesis （DGP）. Methods： Forty-eight healthy Sprague-Dawley （SD） rats were randomly assigned into four groups： a normal group （group A）, a model group （group B）, a group of EA at acupoints （group C）, and a group of EA at non-acupoints （group D）, 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin （STZ） plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate （GER） were observed. The content of insulin （INS） in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay （ELISA）. The expression of growth hormone secretagogue receptor mRNA （GHSR mRNA） in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction （PCR）. Results：Compared with group A, blood glucose and urine glucose increased significantly （P〈0.01）, GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly （P〈0.05 orP〈0.01） in group B. Compared with group B, blood glucose and urine glucose decreased significantly （P〈0.05）, GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly （P〈0.05 orP〈0.01） in group C. Conclusion： EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.
文摘Objective： To observe the effect of point-moxibustion on gastrointestinal motility, mRNA and protein expressions of ghrelin and growth hormone secretagogue receptor la （GHSR-la） in lateral septal nucleus of rats with diabetic 8astroparesis （DGP）, and to investigate the central regulatory mechanism of DGP treatment with point-moxibustion. Methods： Forty SPF-grade Sprague-Dawley （SD） rats were randomly divided into a blank group, a model group, an electroacupuncture （EA） group and a point-moxibustion group, with 10 rats in each group. A DGP rat model was established by intraperitoneal injection of 2% streptozotocin （STZ） with 8-week irregular high-sugar and high-fat diet in the model group, the EA group and the point-moxibustion group, and rats in the blank group were injected intraperitoneally with 0.1 mmoL/L （pH 4.5） citric acid-sodium citrate buffer with 8-week normal diet. Eight weeks later, rats in the EA group were treated by EA at Zusanli （ST 36）, Liangmen （ST 21） and Sanyinjiao （SP 6）; while rats in the point-moxibustion group were treated by point-moxibustion at Zusanli （ST 36）, Liangmen （ST 21） and Sanyinjiao （SP 6） for successive 15 d. Rats in the blank group and the model group were fixed as the control without intervention. After treatment, intestinal propulsion rate and gastric emptying rate were measured. The mRNA and protein expressions of ghrelin and GHSR-la in the lateral septal nucleus were detected by real-time polymerase chain reaction （RT-PCR） and Western blot （WB）. Results： Compared with the blank group, the intestinal propulsion rate and gastric emptying rate of the model group were significantly lower （both P〈0.01）; compared with the model group, the intestinal propulsion rate and gastric emptying rate of the EA group and the point-moxibustion group increased significantly （all P〈0.05）. The mRNA and protein expressions of ghrelin and GHSR-la were lower in the model group than those in the blank group （all P〈0.01）. The mRNA and protein expressions of ghrelin and GHSR-Ia were significantly higher in the EA group and the point-moxibustion group than those in the model group （all P〈0.05）. There were no statistically significant differences between the EA group and the point-moxibustion group （all P〉0.05）. Conclusion： Point-moxibustion at Zusanli （ST 36）, Liangmen （ST 21） and Sanyinjiao （SP 6） can increase the intestinal propulsion rate and gastric emptying rate of DGP rats, and promote the mRNA and protein expressions of ghrelin and GHSR-la in the central nervous system. The mechanism may be related to the activation of 8hrelin pathway in hypothalamic arcuate nucleus-lateral septal nucleus.
