AIM:To investigate the expression of effector protease receptor-1 (EPR-1),proliferative index Ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1...AIM:To investigate the expression of effector protease receptor-1 (EPR-1),proliferative index Ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1 expression and its correlationship with the proliferation and apoptosis indexes.METHODS:Using immmunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) technique,we determined the expression of EPR-1,proliferative index (Ki-67) and apoptotic index (AI) in 120 paraffin-embedded specimens of primary advanced gastric adenocarcinoma as well as lymph node metastasis and agjacent normal tissues.RESULTS:EPR-1 expression was distributed in the cytoplasm of normal gastric mycoderma,carcinoma cells and smooth muscl cells.The plsitive rate of EPR-1 expression in the primary gastric adenocarcinomas,invasion tumor node and lymph node metastasis was 65.83%,55.29% and 68%,respectively.While the positive rate in normal gastric mycoderma and smooth muscle cells was 46.7% and 53.3%,respectively.The average positive rate of Ki-67 in EPR-1-positive tumors was 7.00% which was significantly lower than that of 8.53% in EPR-1-negative tumors,but the average AI in EPR-1-positive tumors was 1.25%,which was significantly higher than that of 1.00% observed in EPR-1-negative tumors.On the other hand,the average positive labeling index for Ki-67(ki-67) in EPR-1-positive lymph node metastasis was 7.65%,which was significantly lower than that of 9.44% observed in EPR-1-negative lymph node metastasis.However,the average AI in EPR-1-positive lymph node metastasis tumors was 0.99%,which was significantly higher than that of .67% observed in EPR-1-negative lymph node metastasis.CONCLUSION:The frequency of EPR-1 exrpession was significantly higher in primary gastric adenocarcinoma and in its lymph node metastasis than that in normal gastric mucosa.Expression of EPR-1 Score is positively correlated with apoptosis index,but is negatively realted with proliferative index.Thus,Weighted EPR-1 Score and EPR-1 expression in gastric adenocarcinoma cells maybe a potential marker in clinical setting.展开更多
The development of bifunctional catalysts for the efficient hydrogenation and acceptorless dehydrogenation of N‐heterocycles is a challenge.In this study,Ru_(2)P/AC effectively promoted reversible transformations bet...The development of bifunctional catalysts for the efficient hydrogenation and acceptorless dehydrogenation of N‐heterocycles is a challenge.In this study,Ru_(2)P/AC effectively promoted reversible transformations between unsaturated and saturated N‐heterocycles affording yields of 98%and 99%,respectively.Moreover,a remarkable enhancement in the reusability of Ru_(2)P/AC was observed compared with other Ru‐based catalysts.According to density functional theory calculations,the superior performance of Ru_(2)P/AC was ascribed to specific synergistic factors,namely geometric and electronic effects induced by P.P greatly reduced the large Ru‐Ru ensembles and finely modified the electronic structures,leading to a low reaction barrier and high desorption ability of the catalyst,further boosting the hydrogenation and acceptorless dehydrogenation processes.展开更多
Graphene has lots of applications, such as field-effect transistors, solar cells and transparent electrodes. In this work, we developed a new donor-acceptor graphene hybrid by covalently bonding a donor phenanthrene-9...Graphene has lots of applications, such as field-effect transistors, solar cells and transparent electrodes. In this work, we developed a new donor-acceptor graphene hybrid by covalently bonding a donor phenanthrene-9-carboxaldehyde (PCA) onto the acceptor graphene (PCA-graphene) via 1,3-dipolar cycloaddition azomethine ylides. The resulting PCA-graphene is soluble in N,N-dimethyformamide (DMF). The optoelectronic device (photoanode) fabricated by spin-coating DMF solution of the hybrids exhibits an enhanced photocurrent under visible irradiation.展开更多
文摘AIM:To investigate the expression of effector protease receptor-1 (EPR-1),proliferative index Ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1 expression and its correlationship with the proliferation and apoptosis indexes.METHODS:Using immmunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) technique,we determined the expression of EPR-1,proliferative index (Ki-67) and apoptotic index (AI) in 120 paraffin-embedded specimens of primary advanced gastric adenocarcinoma as well as lymph node metastasis and agjacent normal tissues.RESULTS:EPR-1 expression was distributed in the cytoplasm of normal gastric mycoderma,carcinoma cells and smooth muscl cells.The plsitive rate of EPR-1 expression in the primary gastric adenocarcinomas,invasion tumor node and lymph node metastasis was 65.83%,55.29% and 68%,respectively.While the positive rate in normal gastric mycoderma and smooth muscle cells was 46.7% and 53.3%,respectively.The average positive rate of Ki-67 in EPR-1-positive tumors was 7.00% which was significantly lower than that of 8.53% in EPR-1-negative tumors,but the average AI in EPR-1-positive tumors was 1.25%,which was significantly higher than that of 1.00% observed in EPR-1-negative tumors.On the other hand,the average positive labeling index for Ki-67(ki-67) in EPR-1-positive lymph node metastasis was 7.65%,which was significantly lower than that of 9.44% observed in EPR-1-negative lymph node metastasis.However,the average AI in EPR-1-positive lymph node metastasis tumors was 0.99%,which was significantly higher than that of .67% observed in EPR-1-negative lymph node metastasis.CONCLUSION:The frequency of EPR-1 exrpession was significantly higher in primary gastric adenocarcinoma and in its lymph node metastasis than that in normal gastric mucosa.Expression of EPR-1 Score is positively correlated with apoptosis index,but is negatively realted with proliferative index.Thus,Weighted EPR-1 Score and EPR-1 expression in gastric adenocarcinoma cells maybe a potential marker in clinical setting.
文摘The development of bifunctional catalysts for the efficient hydrogenation and acceptorless dehydrogenation of N‐heterocycles is a challenge.In this study,Ru_(2)P/AC effectively promoted reversible transformations between unsaturated and saturated N‐heterocycles affording yields of 98%and 99%,respectively.Moreover,a remarkable enhancement in the reusability of Ru_(2)P/AC was observed compared with other Ru‐based catalysts.According to density functional theory calculations,the superior performance of Ru_(2)P/AC was ascribed to specific synergistic factors,namely geometric and electronic effects induced by P.P greatly reduced the large Ru‐Ru ensembles and finely modified the electronic structures,leading to a low reaction barrier and high desorption ability of the catalyst,further boosting the hydrogenation and acceptorless dehydrogenation processes.
基金supported by the National Natural Science Foundation of China (20920102034, 20877076 & 20907056)the National Basic Research Program of China (2010CB933503 & 2007CB613306)
文摘Graphene has lots of applications, such as field-effect transistors, solar cells and transparent electrodes. In this work, we developed a new donor-acceptor graphene hybrid by covalently bonding a donor phenanthrene-9-carboxaldehyde (PCA) onto the acceptor graphene (PCA-graphene) via 1,3-dipolar cycloaddition azomethine ylides. The resulting PCA-graphene is soluble in N,N-dimethyformamide (DMF). The optoelectronic device (photoanode) fabricated by spin-coating DMF solution of the hybrids exhibits an enhanced photocurrent under visible irradiation.
