A new anion receptor bearing phenolic hydroxy group based on 3,5- ditertbutylsalicylaldehyde-p-nitrophenylhydrazone (1) was designed and synthesized. Upon addition of AcO- and F-, the receptor exhibited visible colo...A new anion receptor bearing phenolic hydroxy group based on 3,5- ditertbutylsalicylaldehyde-p-nitrophenylhydrazone (1) was designed and synthesized. Upon addition of AcO- and F-, the receptor exhibited visible color changes from deep yellow to purple. However, no obvious color changes were observed on addition of the other anions tested (H2PO4-, Cl-, Br-, I-). The binding properties of the receptor with anions such as AcO and F- were investigated by UV-Vis and fluorescent titrations. The result indicated that the receptor 1 had a higher affinity to AcO- and F- and a 1:1 host-guest complex was formed through H-bond interactions between 1 and anions.展开更多
Fertilization in flowering plants is completed through several recognitionevents, and the first of which is the recognition of pollen by pistil of female reproductivetissue. Self-incompatibility (SI) is an intraspecif...Fertilization in flowering plants is completed through several recognitionevents, and the first of which is the recognition of pollen by pistil of female reproductivetissue. Self-incompatibility (SI) is an intraspecific reproductive barrier to prevent selfferitilization and widely distributed in flowering plants. In many species, SI shows simplegenetics and is controlled by a single multi-allelic locus, called the S locus. In gametophyticSI (GSI) exemplified by the Solanaceae, Scrophulariaceae and Rosaceae, a class ofribonucleases, called S RNases, have been shown to mediate the stylar expression of SI butnot the pollen expression of SI. The latter appears to be determined by a gene differentfrom those encoding S RNases, often referred to as pollen S gene. The pollen S gene is thecrucial missing part in understanding the biochemical and molecular mechanisms of self andnon-self pollen recognition in flowering plants. Recent genetic analysis of mutationsaffecting the pollen expression of SI has suggested a possible model of how the pollen S geneinteracts with S RNases to achieve self and non-self pollen recognition. Furthermore, wewill present two approaches, S-locus directed transposon tagging and map-based cloning, forcloning the pollen S in Antirrhinum.展开更多
AIM: To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer (mCRC) with the aim of identifying prognostic markers.METHODS: The expression of 44 angiogenesis-...AIM: To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer (mCRC) with the aim of identifying prognostic markers.METHODS: The expression of 44 angiogenesis- secreted factors was measured by a novel cytokine antibody array methodology. The study evaluated vas- cular endothelial growth factor (VEGF) and its soluble vascular endothelial growth factor receptor (sVEGFR)-I protein levels by enzyme immunoassay (EIA) in a panel of 16 CRC cell lines, mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) and vascular en- dothelial growth factor receptor (VEGFR)-2 expressionwas analyzed by flow cytometry.展开更多
Type I interferons (IFN) are well studied cytokines with anti-viral and immune-modulating functions. Type I IFNsare produced following viral infections, but until recently, the mechanisms of viral recognition leading ...Type I interferons (IFN) are well studied cytokines with anti-viral and immune-modulating functions. Type I IFNsare produced following viral infections, but until recently, the mechanisms of viral recognition leading to IFN productionwere largely unknown. Toll like receptors (TLRs) have emerged as key transducers of type I IFN during viral infectionsby recognizing various viral components. Furthermore, much progress has been made in defining the signaling path-ways downstream of TLRs for type I IFN production. TLR7 and TLR9 have become apparent as universally importantin inducing type I IFN during infection with most viruses, particularly by plasmacytoid dendritic cells. New intracellularviral pattern recognition receptors leading to type I IFN production have been identified. Many bacteria can also inducethe up-regulation of these cytokines. Interestingly, recent studies have found a detrimental effect on host cells if type IIFN is produced during infection with the intracellular gram-positive bacterial pathogen, Listeria monocytogenes. Thisreview will discuss the recent advances made in defining the signaling pathways leading to type I IFN production.展开更多
AIM To assess the effects of hepatitis B virus(HBV) on the expression of host α-1,2-mannosidases and determine the underlying mechanisms.METHODS We measured the expression levels of MAN1A1, MAN1A2, MAN1B1, and MAN1C1...AIM To assess the effects of hepatitis B virus(HBV) on the expression of host α-1,2-mannosidases and determine the underlying mechanisms.METHODS We measured the expression levels of MAN1A1, MAN1A2, MAN1B1, and MAN1C1 in cell lines HepG 2.2.15, HepN 10, HepA D38 and Hep G2 by Western blot. Viral antigens(HBs Ag and HBe Ag) in the culture medium were measured using the chemiluminescence method. HBV DNA quantification assays were performed using a commercial real-time PCR kit. Protein levels of human liver tissue α-1,2-mannosidases were also evaluated by Western blot. Plasmids containing seven individual viral genes of HBV(PTT22-HBx, PTT22-HBs, PTT22-pre S2, PTT22-pre S1, PTT22-HBc, PTT22-HBe, and PTT22-HBp) or control plasmids(PTT22-vector) were transfected into Hep G2 cells. MK886(PPARα) and GW9662(PPARγ) inhibitors were used to explore the effects of HBV on α-1,2-mannosidase expression after the PPARα and PPARγ pathways were blocked.RESULTS We showed that the expression of α-1,2-mannosidases was higher in stably transfected HBV cells than in controls. The expression levels of α-1,2-mannosidase were higher in AD38 cells than those in ND10 cells, which were in turn greater than those in G2.2.15 cells, and positively correlated with the expression of HBsA gin all the cell lines. Levels of α-1,2-mannosidase in nontumorous liver tissues of HBV-related HCC patients were also higher than in the tissues from non-HBVrelated HCC patients. Moreover, transfecting Hep G2 cells with a component of the HBV viral envelope also increased the expression of α-1,2-mannosidases. However, this envelope protein component could not induce MAN1C1 expression in the presence of a PPARα inhibitor, MK886. We also found that MK886 did not affect the expression of MAN1C1 in AD38 cells without tetracycline in the culture medium. This phenomenon was not observed in the case of GW9662.CONCLUSION Our results indicate that HBV increases the expression of α-mannosidases both in vitro and in vivo via activation of the PPARα pathway by its envelope protein.展开更多
Anion receptors including pyrimidine subunit were designed and synthesized and their binding abilities with various anions were investigated by fluorescence and ~1H NMR titration experiments.DFT calculations provided ...Anion receptors including pyrimidine subunit were designed and synthesized and their binding abilities with various anions were investigated by fluorescence and ~1H NMR titration experiments.DFT calculations provided some information for anion recognition.It is confirmed that both of two new pyrimidine anion receptors have the selectivity for Cl-.展开更多
The present study investigated the effects of English songs on morphological development-- inflection, derivation, and compound words. Fifty Chinese EFL students were involved in this study. Two tests were administere...The present study investigated the effects of English songs on morphological development-- inflection, derivation, and compound words. Fifty Chinese EFL students were involved in this study. Two tests were administered to each learner immediately before and after the study. Results from the pre-tests revealed that all three groups with different vocabulary sizes brought some morphological knowledge to the study. The findings exhibited some evidence to support the fact that teaching through English songs can lead to the development of learner's receptivity to morphology. However, exposure to the song was insufficient to develop productive knowledge in most of the learners. The study also revealed that groups with smaller vocabulary tended to achieve lower scores than groups with larger vocabulary. Based on these results and previous findings, pedagogical implications were made.展开更多
C-type lectin receptors(CLRs)are powerful pattern-recognition receptors that discern‘self’and‘non-self’in our body and protect us from invasive pathogens by mediating immune recognition and response.The gastrointe...C-type lectin receptors(CLRs)are powerful pattern-recognition receptors that discern‘self’and‘non-self’in our body and protect us from invasive pathogens by mediating immune recognition and response.The gastrointestinal tract is very important for the maintenance of homeostasis;it is the largest shelter for the billions of microorganisms in the body and CLRs play a crucial regulatory role in this system.This study focuses on several CLRs,including Dectin-1,Dectin-2,Dectin-3 and Mincle.We summarize the roles of CLRs in maintaining gastrointestinal immune-system homeostasis,especially their functions in mediating immune recognition and responses in the gut,discuss their relationships to some diseases,highlight the significance of CLR-mediated sensing of microbial and non-microbial compounds in the gut immune system and identify new therapeutic targets.展开更多
Plants are exposed to numerous potential pathogenic microbes. To counter the threat, plants have evolved diverse patternrecognition receptors(PRRs), which are receptor kinases(RKs) and receptor proteins(RPs) specializ...Plants are exposed to numerous potential pathogenic microbes. To counter the threat, plants have evolved diverse patternrecognition receptors(PRRs), which are receptor kinases(RKs) and receptor proteins(RPs) specialized to detect conserved pathogen/microbe-associated molecular patterns(PAMPs/MAMPs). Although only a handful of RKs and RPs are known PRRs,they belong to the receptor-like kinase(RLK) and receptor-like protein(RLP) superfamilies that undergo lineage-specific expansion, suggesting that many of these RLKs and RLPs are potential PRRs. Analyses of existing PRRs have uncovered ligand-induced RLK-RK or RLK-RP oligomerization as a common mechanism for immune activation. PRRs can recruit additional components to form dynamic receptor complexes, which mediate specific cellular responses. Detailed analyses of these components are shedding light on molecular mechanisms underlying the regulation of PRR activity and downstream signaling.展开更多
Diabetic nephropathy(DN)is currently the most common complication of diabetes.It is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic patients.The pathogenesis of DN ...Diabetic nephropathy(DN)is currently the most common complication of diabetes.It is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic patients.The pathogenesis of DN is extremely complex and has not yet been clarified;however,in recent years,increasing evidence has shown the important role of innate immunity in DN pathogenesis.Pattern recognition receptors(PRRs)are important components of the innate immune system and have a significant impact on the occurrence and development of DN.In this review,we classify PRRs into secretory,endocytic,and signal transduction PRRs according to the relationship between the PRRs and subcellular compartments.PRRs can recognize related pathogen-associated molecular patterns(PAMPs)and danger-associated molecular patterns(DAMPs),thus triggering a series of inflammatory responses,promoting renal fibrosis,and finally causing renal impairment.In this review,we describe the proposed role of each type of PRRs in the development and progression of DN.展开更多
基金This work was supported by the Natural Science Foundation of Universities of Inner Mongolia Autonomous Region (No.NG09168 and NG10239).
文摘A new anion receptor bearing phenolic hydroxy group based on 3,5- ditertbutylsalicylaldehyde-p-nitrophenylhydrazone (1) was designed and synthesized. Upon addition of AcO- and F-, the receptor exhibited visible color changes from deep yellow to purple. However, no obvious color changes were observed on addition of the other anions tested (H2PO4-, Cl-, Br-, I-). The binding properties of the receptor with anions such as AcO and F- were investigated by UV-Vis and fluorescent titrations. The result indicated that the receptor 1 had a higher affinity to AcO- and F- and a 1:1 host-guest complex was formed through H-bond interactions between 1 and anions.
文摘Fertilization in flowering plants is completed through several recognitionevents, and the first of which is the recognition of pollen by pistil of female reproductivetissue. Self-incompatibility (SI) is an intraspecific reproductive barrier to prevent selfferitilization and widely distributed in flowering plants. In many species, SI shows simplegenetics and is controlled by a single multi-allelic locus, called the S locus. In gametophyticSI (GSI) exemplified by the Solanaceae, Scrophulariaceae and Rosaceae, a class ofribonucleases, called S RNases, have been shown to mediate the stylar expression of SI butnot the pollen expression of SI. The latter appears to be determined by a gene differentfrom those encoding S RNases, often referred to as pollen S gene. The pollen S gene is thecrucial missing part in understanding the biochemical and molecular mechanisms of self andnon-self pollen recognition in flowering plants. Recent genetic analysis of mutationsaffecting the pollen expression of SI has suggested a possible model of how the pollen S geneinteracts with S RNases to achieve self and non-self pollen recognition. Furthermore, wewill present two approaches, S-locus directed transposon tagging and map-based cloning, forcloning the pollen S in Antirrhinum.
基金Supported by A grant of "Department of Health,Government of Navarra,Spain (23/2009)"
文摘AIM: To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer (mCRC) with the aim of identifying prognostic markers.METHODS: The expression of 44 angiogenesis- secreted factors was measured by a novel cytokine antibody array methodology. The study evaluated vas- cular endothelial growth factor (VEGF) and its soluble vascular endothelial growth factor receptor (sVEGFR)-I protein levels by enzyme immunoassay (EIA) in a panel of 16 CRC cell lines, mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) and vascular en- dothelial growth factor receptor (VEGFR)-2 expressionwas analyzed by flow cytometry.
基金A. K. Perry is supported by the Howard Hughes Medi-cal Institute predoctoral fellowship (Grant No. 59003787).Part of this work was also supported by National Insti-tutes of Health research grants RO1 CA87924, RO1AI056154, and R37 AI47868 to G. Cheng and from the MajorResearch Plan (30170461, 30430640) +1 种基金Natural ScienceFoundation of China, and the National Basic ResearchProgram of MOST (2002CB513001, 2001CB-510002)H. Tang. H. Tang is also a fellow of Outstanding YoungInvestigators of National Naturual Science Foundation ofChina (30025010).
文摘Type I interferons (IFN) are well studied cytokines with anti-viral and immune-modulating functions. Type I IFNsare produced following viral infections, but until recently, the mechanisms of viral recognition leading to IFN productionwere largely unknown. Toll like receptors (TLRs) have emerged as key transducers of type I IFN during viral infectionsby recognizing various viral components. Furthermore, much progress has been made in defining the signaling path-ways downstream of TLRs for type I IFN production. TLR7 and TLR9 have become apparent as universally importantin inducing type I IFN during infection with most viruses, particularly by plasmacytoid dendritic cells. New intracellularviral pattern recognition receptors leading to type I IFN production have been identified. Many bacteria can also inducethe up-regulation of these cytokines. Interestingly, recent studies have found a detrimental effect on host cells if type IIFN is produced during infection with the intracellular gram-positive bacterial pathogen, Listeria monocytogenes. Thisreview will discuss the recent advances made in defining the signaling pathways leading to type I IFN production.
基金Supported by the National Natural Science Foundation of China,No.81171559
文摘AIM To assess the effects of hepatitis B virus(HBV) on the expression of host α-1,2-mannosidases and determine the underlying mechanisms.METHODS We measured the expression levels of MAN1A1, MAN1A2, MAN1B1, and MAN1C1 in cell lines HepG 2.2.15, HepN 10, HepA D38 and Hep G2 by Western blot. Viral antigens(HBs Ag and HBe Ag) in the culture medium were measured using the chemiluminescence method. HBV DNA quantification assays were performed using a commercial real-time PCR kit. Protein levels of human liver tissue α-1,2-mannosidases were also evaluated by Western blot. Plasmids containing seven individual viral genes of HBV(PTT22-HBx, PTT22-HBs, PTT22-pre S2, PTT22-pre S1, PTT22-HBc, PTT22-HBe, and PTT22-HBp) or control plasmids(PTT22-vector) were transfected into Hep G2 cells. MK886(PPARα) and GW9662(PPARγ) inhibitors were used to explore the effects of HBV on α-1,2-mannosidase expression after the PPARα and PPARγ pathways were blocked.RESULTS We showed that the expression of α-1,2-mannosidases was higher in stably transfected HBV cells than in controls. The expression levels of α-1,2-mannosidase were higher in AD38 cells than those in ND10 cells, which were in turn greater than those in G2.2.15 cells, and positively correlated with the expression of HBsA gin all the cell lines. Levels of α-1,2-mannosidase in nontumorous liver tissues of HBV-related HCC patients were also higher than in the tissues from non-HBVrelated HCC patients. Moreover, transfecting Hep G2 cells with a component of the HBV viral envelope also increased the expression of α-1,2-mannosidases. However, this envelope protein component could not induce MAN1C1 expression in the presence of a PPARα inhibitor, MK886. We also found that MK886 did not affect the expression of MAN1C1 in AD38 cells without tetracycline in the culture medium. This phenomenon was not observed in the case of GW9662.CONCLUSION Our results indicate that HBV increases the expression of α-mannosidases both in vitro and in vivo via activation of the PPARα pathway by its envelope protein.
基金Project (21302232) supported by the National Natural Science Foundation of China
文摘Anion receptors including pyrimidine subunit were designed and synthesized and their binding abilities with various anions were investigated by fluorescence and ~1H NMR titration experiments.DFT calculations provided some information for anion recognition.It is confirmed that both of two new pyrimidine anion receptors have the selectivity for Cl-.
基金supported through New Century Higher Education Reform Project of Guangxi(2013JGA446)~~
文摘The present study investigated the effects of English songs on morphological development-- inflection, derivation, and compound words. Fifty Chinese EFL students were involved in this study. Two tests were administered to each learner immediately before and after the study. Results from the pre-tests revealed that all three groups with different vocabulary sizes brought some morphological knowledge to the study. The findings exhibited some evidence to support the fact that teaching through English songs can lead to the development of learner's receptivity to morphology. However, exposure to the song was insufficient to develop productive knowledge in most of the learners. The study also revealed that groups with smaller vocabulary tended to achieve lower scores than groups with larger vocabulary. Based on these results and previous findings, pedagogical implications were made.
基金This work is supported by grants from the National Natural Science Foundation of China(81572354 and 81772542 to T.W.)the Natural Science Foundation of Jiangsu Province in China(BK20161400 to T.W.).
文摘C-type lectin receptors(CLRs)are powerful pattern-recognition receptors that discern‘self’and‘non-self’in our body and protect us from invasive pathogens by mediating immune recognition and response.The gastrointestinal tract is very important for the maintenance of homeostasis;it is the largest shelter for the billions of microorganisms in the body and CLRs play a crucial regulatory role in this system.This study focuses on several CLRs,including Dectin-1,Dectin-2,Dectin-3 and Mincle.We summarize the roles of CLRs in maintaining gastrointestinal immune-system homeostasis,especially their functions in mediating immune recognition and responses in the gut,discuss their relationships to some diseases,highlight the significance of CLR-mediated sensing of microbial and non-microbial compounds in the gut immune system and identify new therapeutic targets.
基金supported by Chinese Ministry of Science and Technology(2015CB910201)Chinese Academy of Sciences Strategic Priority Research Program(XDB11020200)
文摘Plants are exposed to numerous potential pathogenic microbes. To counter the threat, plants have evolved diverse patternrecognition receptors(PRRs), which are receptor kinases(RKs) and receptor proteins(RPs) specialized to detect conserved pathogen/microbe-associated molecular patterns(PAMPs/MAMPs). Although only a handful of RKs and RPs are known PRRs,they belong to the receptor-like kinase(RLK) and receptor-like protein(RLP) superfamilies that undergo lineage-specific expansion, suggesting that many of these RLKs and RLPs are potential PRRs. Analyses of existing PRRs have uncovered ligand-induced RLK-RK or RLK-RP oligomerization as a common mechanism for immune activation. PRRs can recruit additional components to form dynamic receptor complexes, which mediate specific cellular responses. Detailed analyses of these components are shedding light on molecular mechanisms underlying the regulation of PRR activity and downstream signaling.
基金Project supported by the National Natural Science Foundation of China(Nos.81060063 and 81660129).
文摘Diabetic nephropathy(DN)is currently the most common complication of diabetes.It is considered to be one of the leading causes of end-stage renal disease(ESRD)and affects many diabetic patients.The pathogenesis of DN is extremely complex and has not yet been clarified;however,in recent years,increasing evidence has shown the important role of innate immunity in DN pathogenesis.Pattern recognition receptors(PRRs)are important components of the innate immune system and have a significant impact on the occurrence and development of DN.In this review,we classify PRRs into secretory,endocytic,and signal transduction PRRs according to the relationship between the PRRs and subcellular compartments.PRRs can recognize related pathogen-associated molecular patterns(PAMPs)and danger-associated molecular patterns(DAMPs),thus triggering a series of inflammatory responses,promoting renal fibrosis,and finally causing renal impairment.In this review,we describe the proposed role of each type of PRRs in the development and progression of DN.