AIM: Mechanisms underlying the chemopreventive effects of cyclooxygenase (COX) inhibitors remain elusive. We have previously shown that celecoxib but not indomethacin could prevent carcinogen-induced gastric cancer de...AIM: Mechanisms underlying the chemopreventive effects of cyclooxygenase (COX) inhibitors remain elusive. We have previously shown that celecoxib but not indomethacin could prevent carcinogen-induced gastric cancer development in Wistar rats. This chemopreventive effect appeared to be independent of COX-2 and prostaglandin (PG) E2 suppression since the lowest PGE2 was obtained in indomethacin group.This study compared the cell kinetic changes in stomachs of rats after treatment with celecoxib (5, 10, 20 mg/(kg·d)) or indomethacin (3 mg/(kg·d)) to gain more insights into the chemopreventive mechanism.METHODS: The apoptosis and proliferation indexes in gastric tumor, adjacent non-cancer tissues and normal gastric tissues were determined. Apoptosis was quantified by apoptotic nuclei counting and TUNEL, whereas proliferation was determined by Ki67 immunostaining.RESULTS: Treatment with either celecoxib or indomethacin inhibited gastric tumor proliferation by more than 65% (P＜0.02). However, celecoxib caused a dose-dependent increase in apoptosis (P＜0.05) which was not seen in indomethacin-treated tumors (P = 0.54). The highest apoptosis to proliferation ratio was seen in tumors treated with celecoxib at 10 mg/(kg·d). Treatment with this dose of celecoxib was associated with the lowest incidence of gastric cancer development.CONCLUSION: Our findings suggest that the difference in chemopreventive effects of indomethacin and celecoxib in this animal model of gastric carcinogenesis is largely due to the differential cell kinetic changes, which does not correlate with the degree of COX-2 and PG suppression.展开更多
The universal creep equation is successful in relating the creep （ε） to the aging time （t） , coefficient of retardation time （β） , and intrinsic time （ to ）. This relation was used to treat the creep experim...The universal creep equation is successful in relating the creep （ε） to the aging time （t） , coefficient of retardation time （β） , and intrinsic time （ to ）. This relation was used to treat the creep experimental data for polyvinyl chloride （ PVC ） specimens at a given stress and different aging times. The βgs found by the “polynomial fitting” method in this work instead of the “middle - point” method reported in the literature. The unified master line was constructed with the treated data and curves according to the universal equation. The master line can be used to predict the long- term creed behavior and lifetime by extrapolating.展开更多
The influences of time on clays are discussed first,and the concept of the instant normal compression line is proposed by analyzing the existing theories and experimental results.Based on the creep law,the relationshi...The influences of time on clays are discussed first,and the concept of the instant normal compression line is proposed by analyzing the existing theories and experimental results.Based on the creep law,the relationship between the aging time and the overconsolidation parameter is built.With the reloading equation of the UH model(unified hardening model for overconsolidated clays) used to calculate the instant compression deformation,a one-dimensional stress-strain-time relationship is proposed.Furthermore,the evolution of this relationship is analyzed,and the characteristic rate that is a function of the overconsolidation parameter is defined.Then a three-dimensional elastic-viscous-plastic constitutive model is suggested by incorporating equivalent time into the current yield function of the UH model.The new model can describe not only creep,rate effect and other viscous phenomena,but also shear dilatancy,strain softening and other behaviors of overconsolidated clays.Besides,compared with the modified Cam-clay model it requires only one additional parameter(the coefficient of secondary compression) to consider the creep law.Finally,because the proposed model can be changed into the UH model under instantaneous loading,the elastic-plastic and elastic-viscous-plastic frameworks are unified.展开更多
基金Supported by an unrestricted grant From the Hong Kong Society of Digestive Endoscopy and the Natural Science Foundation of Guangdong Province of China(No.010713)
文摘AIM: Mechanisms underlying the chemopreventive effects of cyclooxygenase (COX) inhibitors remain elusive. We have previously shown that celecoxib but not indomethacin could prevent carcinogen-induced gastric cancer development in Wistar rats. This chemopreventive effect appeared to be independent of COX-2 and prostaglandin (PG) E2 suppression since the lowest PGE2 was obtained in indomethacin group.This study compared the cell kinetic changes in stomachs of rats after treatment with celecoxib (5, 10, 20 mg/(kg·d)) or indomethacin (3 mg/(kg·d)) to gain more insights into the chemopreventive mechanism.METHODS: The apoptosis and proliferation indexes in gastric tumor, adjacent non-cancer tissues and normal gastric tissues were determined. Apoptosis was quantified by apoptotic nuclei counting and TUNEL, whereas proliferation was determined by Ki67 immunostaining.RESULTS: Treatment with either celecoxib or indomethacin inhibited gastric tumor proliferation by more than 65% (P＜0.02). However, celecoxib caused a dose-dependent increase in apoptosis (P＜0.05) which was not seen in indomethacin-treated tumors (P = 0.54). The highest apoptosis to proliferation ratio was seen in tumors treated with celecoxib at 10 mg/(kg·d). Treatment with this dose of celecoxib was associated with the lowest incidence of gastric cancer development.CONCLUSION: Our findings suggest that the difference in chemopreventive effects of indomethacin and celecoxib in this animal model of gastric carcinogenesis is largely due to the differential cell kinetic changes, which does not correlate with the degree of COX-2 and PG suppression.
基金Sponsored by the Departmet of Science ad Technology, Government of Heilongjiang Province(Grant No.GC04A407).
文摘The universal creep equation is successful in relating the creep （ε） to the aging time （t） , coefficient of retardation time （β） , and intrinsic time （ to ）. This relation was used to treat the creep experimental data for polyvinyl chloride （ PVC ） specimens at a given stress and different aging times. The βgs found by the “polynomial fitting” method in this work instead of the “middle - point” method reported in the literature. The unified master line was constructed with the treated data and curves according to the universal equation. The master line can be used to predict the long- term creed behavior and lifetime by extrapolating.
基金supported by the National Natural Science Foundation of China (Grant Nos. 51179003,11072016,11272031)the Specialized Research Fund for the Doctoral Program of Higher Education (Grant No.20091102110030)
文摘The influences of time on clays are discussed first,and the concept of the instant normal compression line is proposed by analyzing the existing theories and experimental results.Based on the creep law,the relationship between the aging time and the overconsolidation parameter is built.With the reloading equation of the UH model(unified hardening model for overconsolidated clays) used to calculate the instant compression deformation,a one-dimensional stress-strain-time relationship is proposed.Furthermore,the evolution of this relationship is analyzed,and the characteristic rate that is a function of the overconsolidation parameter is defined.Then a three-dimensional elastic-viscous-plastic constitutive model is suggested by incorporating equivalent time into the current yield function of the UH model.The new model can describe not only creep,rate effect and other viscous phenomena,but also shear dilatancy,strain softening and other behaviors of overconsolidated clays.Besides,compared with the modified Cam-clay model it requires only one additional parameter(the coefficient of secondary compression) to consider the creep law.Finally,because the proposed model can be changed into the UH model under instantaneous loading,the elastic-plastic and elastic-viscous-plastic frameworks are unified.
