Objective: To study the effects of the fixed-point mutant on growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast. Methods: Growth cycle and ultrastructure of the Calmodulin fixed-point mutant ye...Objective: To study the effects of the fixed-point mutant on growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast. Methods: Growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast were studied by using cytometry and scan electron microscope. Results: After culture at 30'C for 36 h, the content of aneuploidy of the temperature-sensitive recombinant yeast monoploid B(F92+) and E(F89+FH1 + ) strains were 63. 87% and 63. 55%, respectively. These were significantly different from control OP<0. 01) ; The content of the non-temperature-sensitive recombinant yeast monoploid A(F89+) and C(F141+) strains were 100. 00% and 99. 97% , respectively, which were not significantly different from control value (P>0. 05). Observed by scan electron microscope, the control group, A and C strain's buds were spherical or oval and those bud-necks were fairly shorter; the B and E strain's buds were slender, some cell were abnormal and some buds might show spherical or oval. Conclusion: DNA division and growth velocity of the temperature-sensitive recombinant B and E strains are slower than those of control group and the non-temperature-sensitive recombinant A and C strains.展开更多
基金Supported by the National Natural Science Foundation of China (No. 39870012)
文摘Objective: To study the effects of the fixed-point mutant on growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast. Methods: Growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast were studied by using cytometry and scan electron microscope. Results: After culture at 30'C for 36 h, the content of aneuploidy of the temperature-sensitive recombinant yeast monoploid B(F92+) and E(F89+FH1 + ) strains were 63. 87% and 63. 55%, respectively. These were significantly different from control OP<0. 01) ; The content of the non-temperature-sensitive recombinant yeast monoploid A(F89+) and C(F141+) strains were 100. 00% and 99. 97% , respectively, which were not significantly different from control value (P>0. 05). Observed by scan electron microscope, the control group, A and C strain's buds were spherical or oval and those bud-necks were fairly shorter; the B and E strain's buds were slender, some cell were abnormal and some buds might show spherical or oval. Conclusion: DNA division and growth velocity of the temperature-sensitive recombinant B and E strains are slower than those of control group and the non-temperature-sensitive recombinant A and C strains.
