The impact of cold rolling deformation,which was introduced after solid solution and before aging treatment,on microstructure evolution and mechanical properties of the as-extruded spray formed Al−9.8Zn−2.3Mg−1.73Cu−0...The impact of cold rolling deformation,which was introduced after solid solution and before aging treatment,on microstructure evolution and mechanical properties of the as-extruded spray formed Al−9.8Zn−2.3Mg−1.73Cu−0.13Cr(wt.%)alloy,was investigated.SEM,TEM,and EBSD were used to analyze the microstructures,and tensile tests were conducted to assess mechanical properties.The results indicate that the D1-T6 sample,subjected to 25%cold rolling deformation,exhibits finer grains(3.35μm)compared to the D0-T6 sample(grain size of 4.23μm)without cold rolling.Cold rolling refines the grains that grow in solution treatment.Due to the combined effects of finer and more dispersed precipitates,higher dislocation density and smaller grains,the yield strength and ultimate tensile strength of the D1-T6 sample can reach 663 and 737 MPa,respectively.In comparison to the as-extruded and D0-T6 samples,the yield strength of the D1-T6 sample increases by 415 and 92 MPa,respectively.展开更多
A commercial wrought Al-Cu-Mg-Mn alloy(2024) was thixoformed based on the recrystallization and partial melting(RAP) route, and the microstructure evolution and segregation behavior during the indirect thixoformin...A commercial wrought Al-Cu-Mg-Mn alloy(2024) was thixoformed based on the recrystallization and partial melting(RAP) route, and the microstructure evolution and segregation behavior during the indirect thixoforming process were studied. The results show that fine spheroidal microstructures can be obtained by partial remelting of commercial extruded 2024 alloys without additional thermomechanical processing. During the indirect thixoforming, the stress distribution can be optimized by increasing the thickness of base region. Under three-dimensional compression stress state, the microstructures are homogeneous among different regions with no evidence of liquid segregation and micro-porosities, and the grains in the columns are deformed plastically. The distribution of tensile mechanical properties is consistent with the microstructures. Moreover, the distribution of deformation mechanism was discussed, and a technical method for improving the stress distribution was proposed.展开更多
A novel rheocasting process, self-inoculation method (SIM), was developed for the microstructure control of semisolid wrought Mg alloy. This process involves mixing between liquid alloy and particles of solid alloy ...A novel rheocasting process, self-inoculation method (SIM), was developed for the microstructure control of semisolid wrought Mg alloy. This process involves mixing between liquid alloy and particles of solid alloy (self-inoculants), subsequently pouring the mixed melt into a special designed multi-stream fluid director. The primary phase with dendritic morphology in the conventionally cast AZ31 alloy has readily transformed into near spherical one in the slurry produced by SIM from melt treatment temperature between 690 ℃ and 710 ℃ and self-inoculants addition of 3%-7%. Achievement of the non-dendritic microstructure at the higher melt treatment temperature requires more self-inoculants addition or decreases in the slope angle of fluid director. Primary phase in the slurry thus produced has attained an ideally globular morphology after isothermal holding at 620 ℃ for 30 s. The increasing holding time leads to decrease of shape factor but the coarsening of particle size. The spheroidization and coarsening evolution process of solid particles during the isothermal holding were analyzed by Lifshitz-Slyozov-Wagner (LSW) theory.展开更多
A sloping semisolid rheo-rolling process of Mg-3Sn-1Mn alloy was developed, and the effects of process parameters on the microstructure and mechanical properties of Mg-3Sn-lMn alloy strip were studied. The results sho...A sloping semisolid rheo-rolling process of Mg-3Sn-1Mn alloy was developed, and the effects of process parameters on the microstructure and mechanical properties of Mg-3Sn-lMn alloy strip were studied. The results show that the primary grain average diameter of the strip increases with the increase of the roll speed. The primary grain average diameter decreases firstly and then increases with the increase of the vibration frequency, and the tensile strength and elongation of the strip increase firstly and then decrease with the increase of the vibration frequency. The primary grain average diameter increases with the increase of casting temperature, and the tensile strength and elongation of the strip decrease correspondingly. When the casting temperature is 670℃, the roll speed is 52 mm/s, and the vibration frequency is 60 Hz, Mg-3Sn-1Mn alloy strip with good properties is produced. The mechanical properties of the present product are higher than those of Mg-3Sn-lMn alloy casting with the addition of 0.87% Ce (mass fraction).展开更多
The microstructural evolution and kinetic characteristics were studied during solution treatment of AM60B Mg alloy prepared by thixoforming. The results indicate that the microstructural evolution includes two stages...The microstructural evolution and kinetic characteristics were studied during solution treatment of AM60B Mg alloy prepared by thixoforming. The results indicate that the microstructural evolution includes two stages: the first stage involves rapid dissolution of eutectic β (Mg 17 Al 12 ) phase, homogenization and coarsening, and the second stage is regarded as normal grain growth consisting of primary α-Mg particles (primary particles) and secondary α-Mg grains (secondary grains). In the first stage, the dissolution completes in a quite short time because the fine β phase can quickly dissolve into the small-sized secondary grains. The homogenization of Al element needs relatively long time. Simultaneously, the microstructure morphology and average grain size obviously change. The first stage sustains approximately 1 h when it is solutionized at 395 ℃ Comparatively, the second stage needs very long time and the microstructure evolves quite slowly as a result of low Al content gradient and thus low diffusivity of Al element after the homogenization of the first stage. The growth model of primary particles obeys power function while that of the secondary grains follows the traditional growth equation in the first stage. In the second stage, both of the primary particles and secondary grains behave a same model controlled by diffusion along grain boundaries and through crystal lattice.展开更多
The microstructural evolution and phase transformations during partial remelting of in-situ Mg2Sip/AM60B composite modified by SiC and Sr were investigated. The results indicate that SiC and Sr are effective for refi...The microstructural evolution and phase transformations during partial remelting of in-situ Mg2Sip/AM60B composite modified by SiC and Sr were investigated. The results indicate that SiC and Sr are effective for refining primary α-Mg grains and Mg2Si particles. After being partially remelted, a semisolid microstructure with small and spheroidal primary α-Mg particles can be obtained. The microstructural evolution during partial remelting can be divided into four stages: the initial rapid coarsening, structural separation, spheroidization and final coarsening, which are essentially caused by the phase transformations of β→α, α+β→L and α→L, α→L, and α→L and L→α, respectively. The Mg2Si particles have not obvious effect on the general microstructural evolution steps, but can slower the evolution progress and change the coarsening mechanism. During partial remelting, Mg2Si particles first become blunt and then become spheroidal because of melting of their edges and corners, and finally are coarsened owing to Ostwald ripening.展开更多
With the global warming of concern,the secondary aluminum stream is becoming an even more important component of aluminum production and is attractive because of its economic and environmental benefits.In this work,re...With the global warming of concern,the secondary aluminum stream is becoming an even more important component of aluminum production and is attractive because of its economic and environmental benefits.In this work,recycling of automotive aluminum is reviewed to highlight environmental benefits of aluminum recycling,use of aluminum alloys in automotive applications,automotive recycling process,and new technologies in aluminum scrap process.Literature survey shows that newly developed techniques such as laser induced breakdown spectroscopy(LIBS) and solid state recycling provide promising alternatives in aluminum scrap process.Compared with conventional remelting and subsequent refinement,solid state recycling utilizing compression and extrusion at room or moderate temperature can result in significant energy savings and higher metal yield.展开更多
The effect of ball milling on the microstructural evolution was investigated during partial remelting of 6061 aluminum alloy prepared by cold-pressing of atomized alloy powders.The results indicate that the microstruc...The effect of ball milling on the microstructural evolution was investigated during partial remelting of 6061 aluminum alloy prepared by cold-pressing of atomized alloy powders.The results indicate that the microstructural evolution of 6061 aluminum alloy can be divided into three stages,the dissolution of eutectic phases and the coarsening and growth behavior of the resulting grains,structural separation and spheroidization of primary particles,and the final coarsening behavior of the particles.Compared with the alloy without ball milling,ball milling accelerates the first stage of microstructural evolution due to the energy stored in the powders,but the latter two stages are slowed down because of the formation of large-sized powders.Moreover,the finer the as-cold-pressed microstructure is,the smaller and more spherical the primary particles in the final semisolid microstructure are.Furthermore,properly elevating the heating temperature is beneficial for obtaining small and spheroidal particles.展开更多
A new method based on principal component analysis (PCA) and support vector machines (SVMs) is proposed for fault diagnosis of mine hoists. PCA is used to extract the principal features associated with the gearbox. Th...A new method based on principal component analysis (PCA) and support vector machines (SVMs) is proposed for fault diagnosis of mine hoists. PCA is used to extract the principal features associated with the gearbox. Then, with the irrelevant gearbox variables removed, the remaining gearbox, the hydraulic system and the wire rope parameters were used as input to a multi-class SVM. The SVM is first trained by using the one class-based multi-class optimization algorithm and it is then applied to fault identification. Comparison of various methods showed the PCA-SVM method successfully removed redundancy to solve the dimensionality curse. These results show that the algorithm using the RBF kernel function for the SVM had the best classification properties.展开更多
Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild...Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.展开更多
Based on the analysis of several objective functions,a new method was proposed.Firstly,the feature of the inclination curve was analyzed.On this basis,the soil could be divided into several blocks with different displ...Based on the analysis of several objective functions,a new method was proposed.Firstly,the feature of the inclination curve was analyzed.On this basis,the soil could be divided into several blocks with different displacements and deformations.Then,the method of the soil division was presented,and the characteristic of single soil block was studied.The displacement of the block had two components:sliding and deformation.Moreover,a new objective function was constructed according to the deformation of the soil block.Finally,the sensitivities of the objective functions by traditional method and the new method were calculated,respectively.The result shows that the new objective function is more sensitive to mechanical parameters and the inversion result is close to that obtained by the large direct shear apparatus.So,this method can be used in slope back analysis and its effectiveness is proved.展开更多
We review the species composition, distribution, and seasonal variation of fouling acorn barnacles in Chinese waters--from Bohai Sea and Yellow Sea to East and South China Seas. Thirty-two species of acorn barnacles w...We review the species composition, distribution, and seasonal variation of fouling acorn barnacles in Chinese waters--from Bohai Sea and Yellow Sea to East and South China Seas. Thirty-two species of acorn barnacles were found, of which, the dominant species are Amphibalanus amphitrite, A. reticulatus, A. variegates, Balanus trigonus, Fistulobalanus kondakovi, Megabalanus tintinnabulum, Striatobalanus amaryllis, and Eurapha withersi in the fouling communities. A. amphitrite is the dominant species in the coastal waters of Bohai Sea and Yellow Sea and A. reticulatus is dominant in the East and South China Seas. The settlement period of fouling acorn barnacles is usually in summer and autumn. From north to south with the decrease of latitude, their settlement period obviously extends, even to the whole year, and the species number also increases. Other environmental factors, such as salinity and distance from shore, also play an important role in the distribution of fouling acorn barnacles.展开更多
The Mg-5Sn-1Ca-xGd (x=0, 1) alloys were chosen to investigate the change in solidification paths, phase formation and mechanical properties. The microstructure of as-cast Mg-5Sn-1Ca alloy is composed of α-Mg, Mg2Sn a...The Mg-5Sn-1Ca-xGd (x=0, 1) alloys were chosen to investigate the change in solidification paths, phase formation and mechanical properties. The microstructure of as-cast Mg-5Sn-1Ca alloy is composed of α-Mg, Mg2Sn and CaMgSn phases. With the addition of Gd, the formation of the Mg2Sn phase is impeded and the CaMgSn phase is refined, whereas the ultimate tensile strength and elongation decrease. The possible reasons for the variation in microstructure and mechanical properties were discussed.展开更多
Some problems connect ed with production of new light-weight filler type are considered for sandwich layers. Constructively, the filler is the folded structure that can be developed on a plane. This feature makes it...Some problems connect ed with production of new light-weight filler type are considered for sandwich layers. Constructively, the filler is the folded structure that can be developed on a plane. This feature makes it possible to produce the filler by isometric t ransformation of thin sheet through local bending without material stretching.Th e main difficulty is that the bending must be carried out along all lines of com plex-shaped marking-out at a time. The problem of shaping can be solved by use of the original shaping device that can be transformed in operation. The herein -presented technology of production makes it possible to fabricate parts with d eep relief using a wide gamut of different materials even as the thin-sheet met al alloys and paper.展开更多
Seasonal shifts play an important role in soil microbial community composition. This study examined the hypothesis that soil microbial community structure would vary with seasonal shifts in the Wuyi Mountains in South...Seasonal shifts play an important role in soil microbial community composition. This study examined the hypothesis that soil microbial community structure would vary with seasonal shifts in the Wuyi Mountains in Southeast China, and that two representative tree species (Castanopisi carlesii and Cunninghamia lanceolata) may have different soil microbial community composition. Phospholipids fatty acid analysis (PLFA) of seasonal shifts and was used to assess the effect vegetation types on soil microbial community structure. A total of 22 different PLFAs were identified from all the soil samples. The bacterial PLFAs accounted for 62.37% of the total PLFAs, followed by fungi (28.94%), and the minimum was actinomycetes (6.41%). Overall, the level of PLFAs in C. carlesii soil was greater than those in C. lanceolata soil, and significant differences were observed in some seasons. The amounts of total, bacteria, actinomycic and fungal PLFAs significantly changed with the seasons and followed a sequence order (summer 〉 autumn 〉 spring 〉 winter). The bacteria/fungi PLFAs and G (+)/G (-) PLFAs of two vegetation types also changed with the seasons and the ratios in summer and autumn were higher than those in spring and winter. The correlation analysis of microbial PLFAs and soil physicochemical properties showed that the total, bacteria, fungal, actinomycic, G (+) and G (-) PLFAs were significantly positive correlation with TOC, TN, TP, TK and moisture content. We concluded that the seasonal shifts and vegetation types affect soil microbial community composition by changing the soil physicochemical properties.展开更多
基金the support from the National Natural Science Foundation of China(No.52271177)the Science and Technology Innovation Leaders Projects in Hunan Province,China(No.2021RC4036).
文摘The impact of cold rolling deformation,which was introduced after solid solution and before aging treatment,on microstructure evolution and mechanical properties of the as-extruded spray formed Al−9.8Zn−2.3Mg−1.73Cu−0.13Cr(wt.%)alloy,was investigated.SEM,TEM,and EBSD were used to analyze the microstructures,and tensile tests were conducted to assess mechanical properties.The results indicate that the D1-T6 sample,subjected to 25%cold rolling deformation,exhibits finer grains(3.35μm)compared to the D0-T6 sample(grain size of 4.23μm)without cold rolling.Cold rolling refines the grains that grow in solution treatment.Due to the combined effects of finer and more dispersed precipitates,higher dislocation density and smaller grains,the yield strength and ultimate tensile strength of the D1-T6 sample can reach 663 and 737 MPa,respectively.In comparison to the as-extruded and D0-T6 samples,the yield strength of the D1-T6 sample increases by 415 and 92 MPa,respectively.
基金Project(51405100) supported by the National Natural Science Foundation of ChinaProject(2014M551233) supported by the Postdoctoral Science Foundation of China+1 种基金Project(HIT.NSRIF.2015112) supported by the Natural Scientific Research Innovation Foundation in Harbin Institute of Technology,ChinaProject(HIT(WH)201313) supported by the Scientific Research Foundation of Harbin Institute of Technology at Weihai,China
文摘A commercial wrought Al-Cu-Mg-Mn alloy(2024) was thixoformed based on the recrystallization and partial melting(RAP) route, and the microstructure evolution and segregation behavior during the indirect thixoforming process were studied. The results show that fine spheroidal microstructures can be obtained by partial remelting of commercial extruded 2024 alloys without additional thermomechanical processing. During the indirect thixoforming, the stress distribution can be optimized by increasing the thickness of base region. Under three-dimensional compression stress state, the microstructures are homogeneous among different regions with no evidence of liquid segregation and micro-porosities, and the grains in the columns are deformed plastically. The distribution of tensile mechanical properties is consistent with the microstructures. Moreover, the distribution of deformation mechanism was discussed, and a technical method for improving the stress distribution was proposed.
基金Project (2007CB613700) supported by the National Basic Research Program of ChinaProject (50964010) supported by the National Natural Science Foundation of China
文摘A novel rheocasting process, self-inoculation method (SIM), was developed for the microstructure control of semisolid wrought Mg alloy. This process involves mixing between liquid alloy and particles of solid alloy (self-inoculants), subsequently pouring the mixed melt into a special designed multi-stream fluid director. The primary phase with dendritic morphology in the conventionally cast AZ31 alloy has readily transformed into near spherical one in the slurry produced by SIM from melt treatment temperature between 690 ℃ and 710 ℃ and self-inoculants addition of 3%-7%. Achievement of the non-dendritic microstructure at the higher melt treatment temperature requires more self-inoculants addition or decreases in the slope angle of fluid director. Primary phase in the slurry thus produced has attained an ideally globular morphology after isothermal holding at 620 ℃ for 30 s. The increasing holding time leads to decrease of shape factor but the coarsening of particle size. The spheroidization and coarsening evolution process of solid particles during the isothermal holding were analyzed by Lifshitz-Slyozov-Wagner (LSW) theory.
基金Project(51222405) supported by the National Science Foundation of Outstanding Young Scholars of ChinaProject(51034002) supported by the National Natural Science Foundation of China+1 种基金Project(132002) supported by the Fok Ying Tong Education FoundationProject (2011CB610405) supported by the National Basic Research Program of China
文摘A sloping semisolid rheo-rolling process of Mg-3Sn-1Mn alloy was developed, and the effects of process parameters on the microstructure and mechanical properties of Mg-3Sn-lMn alloy strip were studied. The results show that the primary grain average diameter of the strip increases with the increase of the roll speed. The primary grain average diameter decreases firstly and then increases with the increase of the vibration frequency, and the tensile strength and elongation of the strip increase firstly and then decrease with the increase of the vibration frequency. The primary grain average diameter increases with the increase of casting temperature, and the tensile strength and elongation of the strip decrease correspondingly. When the casting temperature is 670℃, the roll speed is 52 mm/s, and the vibration frequency is 60 Hz, Mg-3Sn-1Mn alloy strip with good properties is produced. The mechanical properties of the present product are higher than those of Mg-3Sn-lMn alloy casting with the addition of 0.87% Ce (mass fraction).
基金Project(G2007CB613706)supported by the National Basic Research Program of ChinaProject supported by the Development Program for Outstanding Young Teachers in Lanzhou University of Technology, ChinaProject(SKL03004)supported by the Opening Foundation of State Key Laboratory of Advanced Nonferrous Materials, China
文摘The microstructural evolution and kinetic characteristics were studied during solution treatment of AM60B Mg alloy prepared by thixoforming. The results indicate that the microstructural evolution includes two stages: the first stage involves rapid dissolution of eutectic β (Mg 17 Al 12 ) phase, homogenization and coarsening, and the second stage is regarded as normal grain growth consisting of primary α-Mg particles (primary particles) and secondary α-Mg grains (secondary grains). In the first stage, the dissolution completes in a quite short time because the fine β phase can quickly dissolve into the small-sized secondary grains. The homogenization of Al element needs relatively long time. Simultaneously, the microstructure morphology and average grain size obviously change. The first stage sustains approximately 1 h when it is solutionized at 395 ℃ Comparatively, the second stage needs very long time and the microstructure evolves quite slowly as a result of low Al content gradient and thus low diffusivity of Al element after the homogenization of the first stage. The growth model of primary particles obeys power function while that of the secondary grains follows the traditional growth equation in the first stage. In the second stage, both of the primary particles and secondary grains behave a same model controlled by diffusion along grain boundaries and through crystal lattice.
基金Project(G2010CB635106)supported by the National Basic Research Program of ChinaProject(NCET-10-0023)supported by the Program for New Century Excellent Talents in University of ChinaProject supported by the Program for Hongliu Outstanding Talents of Lanzhou University of Technology,China
文摘The microstructural evolution and phase transformations during partial remelting of in-situ Mg2Sip/AM60B composite modified by SiC and Sr were investigated. The results indicate that SiC and Sr are effective for refining primary α-Mg grains and Mg2Si particles. After being partially remelted, a semisolid microstructure with small and spheroidal primary α-Mg particles can be obtained. The microstructural evolution during partial remelting can be divided into four stages: the initial rapid coarsening, structural separation, spheroidization and final coarsening, which are essentially caused by the phase transformations of β→α, α+β→L and α→L, α→L, and α→L and L→α, respectively. The Mg2Si particles have not obvious effect on the general microstructural evolution steps, but can slower the evolution progress and change the coarsening mechanism. During partial remelting, Mg2Si particles first become blunt and then become spheroidal because of melting of their edges and corners, and finally are coarsened owing to Ostwald ripening.
基金Project(FRINAT) supported by the Research Council of Norway (RCN)
文摘With the global warming of concern,the secondary aluminum stream is becoming an even more important component of aluminum production and is attractive because of its economic and environmental benefits.In this work,recycling of automotive aluminum is reviewed to highlight environmental benefits of aluminum recycling,use of aluminum alloys in automotive applications,automotive recycling process,and new technologies in aluminum scrap process.Literature survey shows that newly developed techniques such as laser induced breakdown spectroscopy(LIBS) and solid state recycling provide promising alternatives in aluminum scrap process.Compared with conventional remelting and subsequent refinement,solid state recycling utilizing compression and extrusion at room or moderate temperature can result in significant energy savings and higher metal yield.
基金Project(G2010CB635106)supported by the National Basic Research Program of ChinaProject(NCET-10-0023)supported by the Program for New Century Excellent Talents in University of China+1 种基金Project supported by the Program for Hongliu Outstanding Talents of Lanzhou University of Technology,ChinaProject(2014-07)supported by the Basic Scientific Research Expenses of Gansu University,China
文摘The effect of ball milling on the microstructural evolution was investigated during partial remelting of 6061 aluminum alloy prepared by cold-pressing of atomized alloy powders.The results indicate that the microstructural evolution of 6061 aluminum alloy can be divided into three stages,the dissolution of eutectic phases and the coarsening and growth behavior of the resulting grains,structural separation and spheroidization of primary particles,and the final coarsening behavior of the particles.Compared with the alloy without ball milling,ball milling accelerates the first stage of microstructural evolution due to the energy stored in the powders,but the latter two stages are slowed down because of the formation of large-sized powders.Moreover,the finer the as-cold-pressed microstructure is,the smaller and more spherical the primary particles in the final semisolid microstructure are.Furthermore,properly elevating the heating temperature is beneficial for obtaining small and spheroidal particles.
基金Project 06KJD470182 supported by the Jiangsu Educational Natural Science Foundation of china
文摘A new method based on principal component analysis (PCA) and support vector machines (SVMs) is proposed for fault diagnosis of mine hoists. PCA is used to extract the principal features associated with the gearbox. Then, with the irrelevant gearbox variables removed, the remaining gearbox, the hydraulic system and the wire rope parameters were used as input to a multi-class SVM. The SVM is first trained by using the one class-based multi-class optimization algorithm and it is then applied to fault identification. Comparison of various methods showed the PCA-SVM method successfully removed redundancy to solve the dimensionality curse. These results show that the algorithm using the RBF kernel function for the SVM had the best classification properties.
文摘Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.
基金Projects(2013CB036004,2011CB710601)supported by the National Basic Research Program of ChinaProject(51178468)supported by the National Natural Science Foundation of ChinaProject(CX2011B096)supported by Hunan Provincial Postgraduate Innovation Program,China
文摘Based on the analysis of several objective functions,a new method was proposed.Firstly,the feature of the inclination curve was analyzed.On this basis,the soil could be divided into several blocks with different displacements and deformations.Then,the method of the soil division was presented,and the characteristic of single soil block was studied.The displacement of the block had two components:sliding and deformation.Moreover,a new objective function was constructed according to the deformation of the soil block.Finally,the sensitivities of the objective functions by traditional method and the new method were calculated,respectively.The result shows that the new objective function is more sensitive to mechanical parameters and the inversion result is close to that obtained by the large direct shear apparatus.So,this method can be used in slope back analysis and its effectiveness is proved.
基金Supported by the Special Promotion Program for Guangdong Provincial Ocean and Fishery Technology(No.A201101F03)the National Natural Science Foundation of China(Nos.41176102,31100260)
文摘We review the species composition, distribution, and seasonal variation of fouling acorn barnacles in Chinese waters--from Bohai Sea and Yellow Sea to East and South China Seas. Thirty-two species of acorn barnacles were found, of which, the dominant species are Amphibalanus amphitrite, A. reticulatus, A. variegates, Balanus trigonus, Fistulobalanus kondakovi, Megabalanus tintinnabulum, Striatobalanus amaryllis, and Eurapha withersi in the fouling communities. A. amphitrite is the dominant species in the coastal waters of Bohai Sea and Yellow Sea and A. reticulatus is dominant in the East and South China Seas. The settlement period of fouling acorn barnacles is usually in summer and autumn. From north to south with the decrease of latitude, their settlement period obviously extends, even to the whole year, and the species number also increases. Other environmental factors, such as salinity and distance from shore, also play an important role in the distribution of fouling acorn barnacles.
基金Project(2007CB613704) supported by National Basic Research Program of China
文摘The Mg-5Sn-1Ca-xGd (x=0, 1) alloys were chosen to investigate the change in solidification paths, phase formation and mechanical properties. The microstructure of as-cast Mg-5Sn-1Ca alloy is composed of α-Mg, Mg2Sn and CaMgSn phases. With the addition of Gd, the formation of the Mg2Sn phase is impeded and the CaMgSn phase is refined, whereas the ultimate tensile strength and elongation decrease. The possible reasons for the variation in microstructure and mechanical properties were discussed.
文摘Some problems connect ed with production of new light-weight filler type are considered for sandwich layers. Constructively, the filler is the folded structure that can be developed on a plane. This feature makes it possible to produce the filler by isometric t ransformation of thin sheet through local bending without material stretching.Th e main difficulty is that the bending must be carried out along all lines of com plex-shaped marking-out at a time. The problem of shaping can be solved by use of the original shaping device that can be transformed in operation. The herein -presented technology of production makes it possible to fabricate parts with d eep relief using a wide gamut of different materials even as the thin-sheet met al alloys and paper.
基金supported by the National Natural Science Foundation of China (Grant No.31500443)Fujian Province Department of Education Foundation (Grant No.JA15178)
文摘Seasonal shifts play an important role in soil microbial community composition. This study examined the hypothesis that soil microbial community structure would vary with seasonal shifts in the Wuyi Mountains in Southeast China, and that two representative tree species (Castanopisi carlesii and Cunninghamia lanceolata) may have different soil microbial community composition. Phospholipids fatty acid analysis (PLFA) of seasonal shifts and was used to assess the effect vegetation types on soil microbial community structure. A total of 22 different PLFAs were identified from all the soil samples. The bacterial PLFAs accounted for 62.37% of the total PLFAs, followed by fungi (28.94%), and the minimum was actinomycetes (6.41%). Overall, the level of PLFAs in C. carlesii soil was greater than those in C. lanceolata soil, and significant differences were observed in some seasons. The amounts of total, bacteria, actinomycic and fungal PLFAs significantly changed with the seasons and followed a sequence order (summer 〉 autumn 〉 spring 〉 winter). The bacteria/fungi PLFAs and G (+)/G (-) PLFAs of two vegetation types also changed with the seasons and the ratios in summer and autumn were higher than those in spring and winter. The correlation analysis of microbial PLFAs and soil physicochemical properties showed that the total, bacteria, fungal, actinomycic, G (+) and G (-) PLFAs were significantly positive correlation with TOC, TN, TP, TK and moisture content. We concluded that the seasonal shifts and vegetation types affect soil microbial community composition by changing the soil physicochemical properties.