Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopusjaponicus. In this study, four monoclonal antibodies （MAbs） （3C1, 3D9, 2F2, 2A8） against strain AP629 were devel...Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopusjaponicus. In this study, four monoclonal antibodies （MAbs） （3C1, 3D9, 2F2, 2A8） against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only, showing no cross reactivity to other gram-negative bacteria, However, 2F2 and 2A8 showed cross reactivity to all tested bacteria. Indirect immunofluorescence, and immunogold electron microscopy, showed the binding antigens of 3C1 and 3D9 were located at the secretion on the surface of strain AP629. The binding antigens of 2F2 and 2A8 were noted on the membrane of the cells. MAbs 3C1 and 3D9 recognized the lipopolysaccharide fraction of strain AP629, and 2F2 and 2A8 recognized in western-blotting protein antigens with molecular weights of 113 and 128 kDa respectively. MAbs 3C1 and 3D9 have the potential for use in pathogen diagnosis, epidemiology and studies on the mechanism of how S. marisflavi infects A. japonicus. Imrnunohistochemistry with 3C1 or 3D9 identified strain AP629 in the body wall of infected A. japonicus. In the adult sea cucumbers that were infected via body wall injection, positive signals were observed at the site of skin ulceration, and at the connective tissue of the non-ulcerated body wall. In addition, some large blue-stained cells aggregated at the connective tissue colonized by large numbers of bacteria. In juveniles infected via immersion infection, positive signals were observed at the cuticle of the body wall only. Our results suggest that 3C1 and 3D9 could be used in various immunological assays to study the invasion mechanism of strain AP629 in A. japonicus, the law of bacterial colonization, proliferation in different tissues of A. japonicus, and correlation between secretion on the surface of strain AP629 and its pathogenesis to A. japonicus.展开更多
AIM: To assess the significance of phosphatidylinositol 3-kinase (PI3K) in colorectal cancer (CRC) and toxicity of LY294002 in CRC cells with different metastatic abilities. METHODS: Sixty formalin-fixed and paraffin-...AIM: To assess the significance of phosphatidylinositol 3-kinase (PI3K) in colorectal cancer (CRC) and toxicity of LY294002 in CRC cells with different metastatic abilities. METHODS: Sixty formalin-fixed and paraffin-embedded CRC tumor specimens were investigated. Adjacent normal colonic mucosa specimens from 10 of these cases were selected as controls. PI3K protein was detected by immunohistochemistry and PIK3CA mutations were investigated by gene sequencing analysis. A flowcytometry-based apoptosis detection kit was used to determine PI3K inhibitor-induced apoptosis in CRC cell lines SW480 and SW620. Expression of phosphorylated protein kinase B in CRC cell lines was detected by Western blotting. RESULTS: There was a significant difference in the proportion of primary lesions (30%, 18/60) vs metastatic lesions (46.7%, 28/60) that were positive for PI3K (P < 0.05). Mutations were detected in exon 9 (13.3%) and exon 20 (8.3%). Out of 60 cases, seven mutations were identified: two hotspot mutations, C.1633G>A resulting in E545A, and C.3140A>G resulting in H1047R; two novel missense mutations C.1624G>A and C.3079G>A; and three synonymous mutations (C.1641G>A, C.1581C>T and C.3027T>A). Exposure of SW480 cells to PI3K inhibitor for 48 h resulted in a significant increase of apoptotic cells in a dose-dependent manner [3.2% apoptotic cells in 0 μmol/L, 4.3% in 5 μmol/L, 6.3% in 10 μmol/L (P < 0.05), and 6.7% in 20 μmol/L (P < 0.05)]. Moreover, PI3K inhibitor induced a similar significant increase of apoptotic cells in the SW620 cell line for 48 h [3.3% apoptotic cells in 0 μmol/L, 13.3% in 5 μmol/L (P < 0.01), 19.2% in 10 μmol/L (P < 0.01), and 21.3% in 20 μmol/L (P < 0.01)]. CONCLUSION: High PI3K expression is associated with CRC metastasis. PI3K inhibitor induced apoptosis in CRC cells and displayed strong cytotoxicity for highly metastatic cells. PI3K inhibition may be an effective treatment for CRC.展开更多
The motion track of belt spindle is important for the radial error of belt spindle. An analytical modeling method for the motion track of belt spindle under the combined effects of bending moment-torque-thermal deform...The motion track of belt spindle is important for the radial error of belt spindle. An analytical modeling method for the motion track of belt spindle under the combined effects of bending moment-torque-thermal deformation is proposed in this paper. Three running phases of belt spindle have been analyzed and modeled: the start-up phase with leaping change due to the change in force and bending moment, the accelerating phase with axis deflection, and the constant speed phase with axis regression because of the combined effects of bending moment-torque-thermal deformation. The simulation and test were completed on the belt spindle of SKVM850 machine tool, which illustrates the variation law of the radial error of belt spindle during the whole running phases.展开更多
AIM:To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.METHODS:A ...AIM:To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.METHODS:A series of gastric cancer cell lines resistant to different concentrations of adriamycin was established,and the expression of PrPc,Bcl-2 and Bax was detected in these cells.Apoptosis was determined using Annexin V staining.Western blotting and immunohisto-chemistry were performed to detect the expression of PrPc in patients receiving chemotherapy and to explore the role of PrPc expression in predicting the chemosensitivity and the outcome of gastric cancer patients receiving chemotherapy.Follow-up was performed for 2 years.RESULTS:PrPc expression was increased with the increase in drug resistance.Bcl-2,together with PrPc,increased the level of anti-apoptosis of cancer cells.Increased PrPc expression predicted the enhanced level of anti-apoptosis and resistance to anticancer drugs.PrPc expression could be used as a marker for predicting the efficacy of chemotherapy and the prognosis of gastric cancer.Increased PrPc expression predicted both poor chemosensitivity and a low 2-year survival rate.Contrarily,low PrPc expression predicted favorable chemosensitivity and a relatively high 2-year survival rate.CONCLUSION:PrPc expression is associated with histological types and differentiation of gastric cancer cells;The PrPc expression level might be a valuable marker in predicting the efficacy of chemotherapy and the prognosis of gastric cancer patients receiving chemotherapy.展开更多
To improve traffic performance when on-ramp vehicles merge into the mainstream,a collaborative merging control strategy is proposed to determine the merging sequence and trajectory control of vehicles.Merging trajecto...To improve traffic performance when on-ramp vehicles merge into the mainstream,a collaborative merging control strategy is proposed to determine the merging sequence and trajectory control of vehicles.Merging trajectory planning takes the minimization of vehicle acceleration as the optimization objective.Either the variational method or the quadratic programming method is utilized to determine arrival time,optimal time and control variables for each vehicle.As a supplement,the adaptive cruise control(ACC)model is used to calculate each control variable in each time interval on special occasions.Simulation results show that the cooperative merging control strategy outperforms the optimal control strategy.The root mean square(RMS)of acceleration and the root mean square error(RMSE)of time headway are significantly decreased,with the reductions up to 90.1%and 25.2%,respectively.Under the cooperative control strategy,the difference between the average speed and desired speed consistently approaches zero.In addition,few or no collisions occur.To conclude,the proposed strategy favours the improvements in passenger comfort,traffic efficiency,traffic stability and safety around highway on-ramps.展开更多
Y2001-62853-1830 0206829实时合成孔径雷达处理机1阶运动补偿的再取样=Resample in the first order motion compensation of realtime SAR processor[会,英]/Guo,Y.-M,& Chen,H.//2000 5<sup>th</sup> International ...Y2001-62853-1830 0206829实时合成孔径雷达处理机1阶运动补偿的再取样=Resample in the first order motion compensation of realtime SAR processor[会,英]/Guo,Y.-M,& Chen,H.//2000 5<sup>th</sup> International Conference on signal Process-ing Proceedings,Vol.Ⅲ of Ⅲ.—1830～1833（HE）展开更多
The existing spatially variant apodizations(SVAs) either cannot depress the sidelobes effectively or reduce the energy of the mainlobe.To improve this,a modified SVA(MSVA) is put forward in this paper,which expands th...The existing spatially variant apodizations(SVAs) either cannot depress the sidelobes effectively or reduce the energy of the mainlobe.To improve this,a modified SVA(MSVA) is put forward in this paper,which expands the traditional filter from 3-taps to 5-taps and sets relevant parameters according to different sampling rates to get the excellent result that satisfies constrained optimization theory.A modified super-SVA is also presented,which compares the result after the iteration with the original signal and makes the one whose amplitude is smaller as the initial value of the next iteration.This method can eliminate the sidelobes produced by the intermediate operation,so that the following bandwidth extrapolation is more available.Super-MSVA is presented based on the modified SVA and modified super-SVA,which is suitable for any Nyquist sampling rate,can extrapolate the signal bandwidth many times through iteration with a commensurate improvement in resolution,as demonstrated by the result of the experiment.展开更多
基金Supported by the National Natural Science Foundation of China (Nos. 30800853 and 30901107)the National Key Projects, National Science and Technology Pillar Program during the 12th Five-Year-Plan (No. 2011BAD13B03)
文摘Shewanella marisflavi strain AP629 was certified as a novel pathogen of the sea cucumber Apostichopusjaponicus. In this study, four monoclonal antibodies （MAbs） （3C1, 3D9, 2F2, 2A8） against strain AP629 were developed by immunizing Balb/C mice. 3C1 and 3D9 recognized S. marisflavi only, showing no cross reactivity to other gram-negative bacteria, However, 2F2 and 2A8 showed cross reactivity to all tested bacteria. Indirect immunofluorescence, and immunogold electron microscopy, showed the binding antigens of 3C1 and 3D9 were located at the secretion on the surface of strain AP629. The binding antigens of 2F2 and 2A8 were noted on the membrane of the cells. MAbs 3C1 and 3D9 recognized the lipopolysaccharide fraction of strain AP629, and 2F2 and 2A8 recognized in western-blotting protein antigens with molecular weights of 113 and 128 kDa respectively. MAbs 3C1 and 3D9 have the potential for use in pathogen diagnosis, epidemiology and studies on the mechanism of how S. marisflavi infects A. japonicus. Imrnunohistochemistry with 3C1 or 3D9 identified strain AP629 in the body wall of infected A. japonicus. In the adult sea cucumbers that were infected via body wall injection, positive signals were observed at the site of skin ulceration, and at the connective tissue of the non-ulcerated body wall. In addition, some large blue-stained cells aggregated at the connective tissue colonized by large numbers of bacteria. In juveniles infected via immersion infection, positive signals were observed at the cuticle of the body wall only. Our results suggest that 3C1 and 3D9 could be used in various immunological assays to study the invasion mechanism of strain AP629 in A. japonicus, the law of bacterial colonization, proliferation in different tissues of A. japonicus, and correlation between secretion on the surface of strain AP629 and its pathogenesis to A. japonicus.
基金Supported by Youth Foundation of Shanghai Municipal Health Bureau, No. 2008Y087Jiangsu University Clinical Medicine Science and Technology Development Fund, No. JLY20080090
文摘AIM: To assess the significance of phosphatidylinositol 3-kinase (PI3K) in colorectal cancer (CRC) and toxicity of LY294002 in CRC cells with different metastatic abilities. METHODS: Sixty formalin-fixed and paraffin-embedded CRC tumor specimens were investigated. Adjacent normal colonic mucosa specimens from 10 of these cases were selected as controls. PI3K protein was detected by immunohistochemistry and PIK3CA mutations were investigated by gene sequencing analysis. A flowcytometry-based apoptosis detection kit was used to determine PI3K inhibitor-induced apoptosis in CRC cell lines SW480 and SW620. Expression of phosphorylated protein kinase B in CRC cell lines was detected by Western blotting. RESULTS: There was a significant difference in the proportion of primary lesions (30%, 18/60) vs metastatic lesions (46.7%, 28/60) that were positive for PI3K (P < 0.05). Mutations were detected in exon 9 (13.3%) and exon 20 (8.3%). Out of 60 cases, seven mutations were identified: two hotspot mutations, C.1633G>A resulting in E545A, and C.3140A>G resulting in H1047R; two novel missense mutations C.1624G>A and C.3079G>A; and three synonymous mutations (C.1641G>A, C.1581C>T and C.3027T>A). Exposure of SW480 cells to PI3K inhibitor for 48 h resulted in a significant increase of apoptotic cells in a dose-dependent manner [3.2% apoptotic cells in 0 μmol/L, 4.3% in 5 μmol/L, 6.3% in 10 μmol/L (P < 0.05), and 6.7% in 20 μmol/L (P < 0.05)]. Moreover, PI3K inhibitor induced a similar significant increase of apoptotic cells in the SW620 cell line for 48 h [3.3% apoptotic cells in 0 μmol/L, 13.3% in 5 μmol/L (P < 0.01), 19.2% in 10 μmol/L (P < 0.01), and 21.3% in 20 μmol/L (P < 0.01)]. CONCLUSION: High PI3K expression is associated with CRC metastasis. PI3K inhibitor induced apoptosis in CRC cells and displayed strong cytotoxicity for highly metastatic cells. PI3K inhibition may be an effective treatment for CRC.
基金Supported by the State S&T Projects for Upmarket NC Machine and Fundamental Manufacturing Equipments of China(No.2012ZX04012-031)
文摘The motion track of belt spindle is important for the radial error of belt spindle. An analytical modeling method for the motion track of belt spindle under the combined effects of bending moment-torque-thermal deformation is proposed in this paper. Three running phases of belt spindle have been analyzed and modeled: the start-up phase with leaping change due to the change in force and bending moment, the accelerating phase with axis deflection, and the constant speed phase with axis regression because of the combined effects of bending moment-torque-thermal deformation. The simulation and test were completed on the belt spindle of SKVM850 machine tool, which illustrates the variation law of the radial error of belt spindle during the whole running phases.
基金Supported by National Natural Science Foundation of China, No. 30672063China Postdoctoral Science Foundation Funded Project,No. 20080431404China Postdoctoral Special Fund,No. 200801038
文摘AIM:To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.METHODS:A series of gastric cancer cell lines resistant to different concentrations of adriamycin was established,and the expression of PrPc,Bcl-2 and Bax was detected in these cells.Apoptosis was determined using Annexin V staining.Western blotting and immunohisto-chemistry were performed to detect the expression of PrPc in patients receiving chemotherapy and to explore the role of PrPc expression in predicting the chemosensitivity and the outcome of gastric cancer patients receiving chemotherapy.Follow-up was performed for 2 years.RESULTS:PrPc expression was increased with the increase in drug resistance.Bcl-2,together with PrPc,increased the level of anti-apoptosis of cancer cells.Increased PrPc expression predicted the enhanced level of anti-apoptosis and resistance to anticancer drugs.PrPc expression could be used as a marker for predicting the efficacy of chemotherapy and the prognosis of gastric cancer.Increased PrPc expression predicted both poor chemosensitivity and a low 2-year survival rate.Contrarily,low PrPc expression predicted favorable chemosensitivity and a relatively high 2-year survival rate.CONCLUSION:PrPc expression is associated with histological types and differentiation of gastric cancer cells;The PrPc expression level might be a valuable marker in predicting the efficacy of chemotherapy and the prognosis of gastric cancer patients receiving chemotherapy.
基金The Scientific Innovation Research of Graduate Students in Jiangsu Province(No.KYCX17_0145,KYCX17_0141)
文摘To improve traffic performance when on-ramp vehicles merge into the mainstream,a collaborative merging control strategy is proposed to determine the merging sequence and trajectory control of vehicles.Merging trajectory planning takes the minimization of vehicle acceleration as the optimization objective.Either the variational method or the quadratic programming method is utilized to determine arrival time,optimal time and control variables for each vehicle.As a supplement,the adaptive cruise control(ACC)model is used to calculate each control variable in each time interval on special occasions.Simulation results show that the cooperative merging control strategy outperforms the optimal control strategy.The root mean square(RMS)of acceleration and the root mean square error(RMSE)of time headway are significantly decreased,with the reductions up to 90.1%and 25.2%,respectively.Under the cooperative control strategy,the difference between the average speed and desired speed consistently approaches zero.In addition,few or no collisions occur.To conclude,the proposed strategy favours the improvements in passenger comfort,traffic efficiency,traffic stability and safety around highway on-ramps.
文摘Y2001-62853-1830 0206829实时合成孔径雷达处理机1阶运动补偿的再取样=Resample in the first order motion compensation of realtime SAR processor[会,英]/Guo,Y.-M,& Chen,H.//2000 5<sup>th</sup> International Conference on signal Process-ing Proceedings,Vol.Ⅲ of Ⅲ.—1830～1833（HE）
基金supported by the Knowledge Innovative Program of the Chinese Academy of Sciences (Grant No.053Z170138)
文摘The existing spatially variant apodizations(SVAs) either cannot depress the sidelobes effectively or reduce the energy of the mainlobe.To improve this,a modified SVA(MSVA) is put forward in this paper,which expands the traditional filter from 3-taps to 5-taps and sets relevant parameters according to different sampling rates to get the excellent result that satisfies constrained optimization theory.A modified super-SVA is also presented,which compares the result after the iteration with the original signal and makes the one whose amplitude is smaller as the initial value of the next iteration.This method can eliminate the sidelobes produced by the intermediate operation,so that the following bandwidth extrapolation is more available.Super-MSVA is presented based on the modified SVA and modified super-SVA,which is suitable for any Nyquist sampling rate,can extrapolate the signal bandwidth many times through iteration with a commensurate improvement in resolution,as demonstrated by the result of the experiment.
