[Objective] This study aimed to establish a method for determining the content of Astragaloside IV in Yupingfeng oral solution.[Method] The HPLC-ELSD method was adopted.The chromatographic column was Venusil MP(4.6 m...[Objective] This study aimed to establish a method for determining the content of Astragaloside IV in Yupingfeng oral solution.[Method] The HPLC-ELSD method was adopted.The chromatographic column was Venusil MP(4.6 mm × 150 mm,5 μm).The mobile phase was acetonitrile-water(35∶65).The ELSD evaporator tube temperature was 65 ℃.N2 was used as the carrier gas(pressure,30 psi).[Result] When the content of Astragaloside IV ranged from 0.5 to 5.0 μg,the Astragaloside IV content showed a good linear relationship with peak area(r=0.999,n=6).The average recovery was 96.36%,and the RSD was 2.46%.[Conclusion] This method is accurate and reliable,and can be applied in the quality control of Yupingfeng oral solution.展开更多
In order to study the diurnal variation of soil CO2 effiux from temperate meadow steppes in Northeast China, and determine the best time for observation, a field experiment was conducted with a LI-6400 soil CO2 flux s...In order to study the diurnal variation of soil CO2 effiux from temperate meadow steppes in Northeast China, and determine the best time for observation, a field experiment was conducted with a LI-6400 soil CO2 flux system under five typical plant communi- ties (Suaeda glauca (Sg), Chloris virgata (Cv), Puecinellia distans (Pd), Leymus chinensis (Lc) and Phragmites australis (Pa)) and an alkali-spot land (As) at the meadow steppe of western Songnen Plain. The results showed that the diurnal variation of soil CO2 effiux exhibited a single peak curve in the growing season. Diurnal maximum soil respiration (Rs) often appeared between 1 1:00 and 13:00, while the minimum occurred at 21:00-23:00 or before dawn. Air temperature near the soil surface (Ta) and soil temperature at 10 cm depth (Tlo) exerted dominant control on the diurnal variations of soil respiration. The time-windows 7:00-9:00 could be used as the optimal measuring time to represent the daily mean soil CO2 effiux at the Cv, Pd, Lc and Pa sites. The daily mean soil CO2 effiux was close to the soil COz effiux from 15:00 to 17:00 and the mean of 2 individual soil CO2 effiux from 15:00 to 19:00 at the As and Sg sites, respectively. During nocturnal hours, negative soil CO2 fluxes (CO2 downwards into the soil) were frequently observed at the As and Sg sites, the magnitude of the negative CO2 fluxes were 0.10-1.55 gmol/(m2.s) and 0.10-0.69 gmol/(m2.s)at the two sites. The results im- plied that alkaline soils could absorb CO2 under natural condition, which might have significant implications to the global carbon budget accounting.展开更多
Traffic flow prediction is an important component for real-time traffic-adaptive signal control in urban arterial networks.By exploring available detector and signal controller information from neighboring intersectio...Traffic flow prediction is an important component for real-time traffic-adaptive signal control in urban arterial networks.By exploring available detector and signal controller information from neighboring intersections,a dynamic data-driven flow prediction model was developed.The model consists of two prediction components based on the signal states(red or green) for each movement at an upstream intersection.The characteristics of each signal state were carefully examined and the corresponding travel time from the upstream intersection to the approach in question at the downstream intersection was predicted.With an online turning proportion estimation method,along with the predicted travel times,the anticipated vehicle arrivals can be forecasted at the downstream intersection.The model performance was tested at a set of two signalized intersections located in the city of Gainesville,Florida,USA,using the CORSIM microscopic simulation package.Analysis results show that the model agrees well with empirical arrival data measured at 10 s intervals within an acceptable range of 10%-20%,and show a normal distribution.It is reasonably believed that the model has potential applicability for use in truly proactive real-time traffic adaptive signal control systems.展开更多
The visual inspection is an economical and effective method for welding. For measuring the feature sizes of grooves,a method based on line structured light is presented. Firstly,an adaptive algorithm to extract the su...The visual inspection is an economical and effective method for welding. For measuring the feature sizes of grooves,a method based on line structured light is presented. Firstly,an adaptive algorithm to extract the subpixel centerline of structured light stripes is introduced to deal with the uneven width and grayscale distributions of laser stripes,which is based on the quadratic weighted grayscale centroid. By means of region-of-interest(ROI)division and image difference,an image preprocessing algorithm is developed for filtering noise and improving image quality. Furthermore,to acquire geometrical dimensions of various grooves and groove types precisely,the subpixel feature point extraction algorithm of grooves is designed. Finally, experimental results of feature size measuring show that the absolute error of measurement is 0.031—0.176 mm,and the relative error of measurement is 0.2%—3.6%.展开更多
To automatically detecting whether a person is wearing mask properly,we propose a face mask detection algorithm based on hue-saturation-value(HSV)+histogram of oriented gradient(HOG)features and support vector machine...To automatically detecting whether a person is wearing mask properly,we propose a face mask detection algorithm based on hue-saturation-value(HSV)+histogram of oriented gradient(HOG)features and support vector machines(SVM).Firstly,human face and five feature points are detected with RetinaFace face detection algorithm.The feature points are used to locate to mouth and nose region,and HSV+HOG features of this region are extracted and input to SVM for training to realize detection of wearing masks or not.Secondly,RetinaFace is used to locate to nasal tip area of face,and YCrCb elliptical skin tone model is used to detect the exposure of skin in the nasal tip area,and the optimal classification threshold can be found to determine whether the wear is properly according to experimental results.Experiments show that the accuracy of detecting whether mask is worn can reach 97.9%,and the accuracy of detecting whether mask is worn correctly can reach 87.55%,which verifies the feasibility of the algorithm.展开更多
The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), a...The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), alternative moving window factor analysis (AMWFA) algorithms and normalization method based on the peak areas; the flavones in the barks and leaves of Eucommia ulmoides Oliver were separated on an ODS column by gradient elution carried out with the flow phase consisting of water, methanol and phosphoric acid (0.1%), and their contents were quantitatively determined by standard curve method and diode array detection (DAD) at 362 nm. The results show that 68 and 73 compounds respectively from essential oils of the barks and leaves of Eucommia ulmoides Oliver are identified, and there are 33 mutual compounds among 108 compounds determined. The total contents of these volatile components of the two samples possess 92.9% and 97.75% of the gross of the relevant essential oils, respectively; the contents of the rutin, quercetin and kaempferol in the barks and leaves of Eucommia ulmoides Oliver are 0.016 9, 0.003 6, 0.002 1 and 0.064 4, 0.030 2, 0.010 0 mg/g, respectively, and the determination recoveries are 95.2%-106.2%. The comparative analysis shows that for the barks and leaves of Eucommia ulmoides Oliver, there are significant differences in their components of the relevant essential oils and flavones.展开更多
It is important to quantify the effect of the root diameter, the embedment length of the root and load speed on the soil-root interface mechanical properties for studying the root anchorage. The soilroot interface mec...It is important to quantify the effect of the root diameter, the embedment length of the root and load speed on the soil-root interface mechanical properties for studying the root anchorage. The soilroot interface mechanical properties can be obtained through the pullout force and root slippage curve(F-S curve). About 120 Pinus tabulaeformis single roots whose diameters ranged from 1 mm to 10 mm divided into 6 groups based on different root embedment length(50 mm, 100 mm and 150 mm) and different load velocity(10 mm·min^(-1), 50 mm·min^(-1), 100 mm·min^(-1) and 300 mm·min^(-1)) were investigated using the pullout method. This study aims to explore the mechanical properties of the soil-root interface in the real conditions using the pullout test method. The results showed two kinds of pullout test failure modes during the experimental process: breakage failure and pullout failure. The results showed that the roots were easier to be broken when the root diameter was smaller or the loading speed was larger. The relationship between the maximum anchorage force and root diameter was linear and the linearly dependent coefficient(R^2) was larger than 0.85. The anchorage force increased with the root embedment length. An increase of 10%^(-1)5% for the maximumanchorage force was found when load speed increased from 10 to 300 mm.min^(-1). The mean peak slippage of the root was from 13.81 to 35.79 mm when the load velocity varied from 10 to 300 mm.min^(-1). The study will be helpful for the design of slopes reinforced by vegetation and in predicting risk of uprooting of trees, and will have practical benefits for understanding the mechanism of landslide.展开更多
AIM: To characterize changes in ghrelin levels in response to oral glucose tolerance test (OGTT) and to correlate changes in ghrelin levels with changes in insulin and glucose following OGTT in Chinese obese childr...AIM: To characterize changes in ghrelin levels in response to oral glucose tolerance test (OGTT) and to correlate changes in ghrelin levels with changes in insulin and glucose following OGTT in Chinese obese children of Tanner Ⅰ and Ⅱ stage with insulin resistance. METHODS: 22 obese children with insulin resistance state were divided into four groups according to their Tanner stage and gender: boys of Tanner Ⅰ (fir- Ⅰ ), boys of Tanner Ⅱ(BT-Ⅱ ), girls of Tanner Ⅰ (GT- Ⅰ ), girls of Tanner Ⅱ (GT-Ⅱ). Ghrelin, insulin and glucose were measured at 0, 30, 60 and 120 rain following OGTT. The control children with normal BMI were divided into control boys of Tanner Ⅰ (CBT- Ⅰ, n = 6), control boys of Tanner Ⅱ (CBT-Ⅱ, n = 5), control girls of Tanner Ⅰ (CGT- Ⅰ, n = 6), control girls of Tanner Ⅱ (CGT-Ⅱ, n = 5). Fasting serum ghrelin levels were analyzed. RESULTS: Ghrelin levels were lower in obese groups. Ghrelin levels of control group decreased in Tanner Ⅱ stage (CGT- Ⅰ vs CGT-Ⅱ t = -4.703, P = 0.001; CBT- Ⅰ vs CBT- Ⅱ t = -4.794, P = 0.001). Basal ghrelin levels in fir-Ⅱ decreased more significantly than that in BT- Ⅰ group (t = 2.547, P = 0.029). Ghrelin levels expressed a downward trend after OGTT among obese children. The decrease in ghrelin levels at 60 min with respect to basal values was 56.9% in BT- Ⅰ. Ghrelin concentrations at 0 min correlated directly with glucose level at 0 min in fir- Ⅰ (r = 0.898, P = 0.015). There wasn't a significant correlation of ghrelin changes with glucose changes and insulin changes during OGTT in obese children with insulin resistance. CONCLUSION: In conclusion, in obese children with insulin resistance, ghrelin levels decreased with advancing pubertal stage. Ghrelin secretion suppression following OGTT was influenced by gender and pubertal stage. Baseline ghrelin levels and ghrelin suppression after OGTT did not significantly correlate with the degree of insulin resistance and insulin sensitivity.展开更多
AIM: TO evaluate the accuracy of two non-invasive tests in a population of Alaska Native persons. High rates of Helicobacter pylori (H. pylon] infection, H. pyIori treatment failure, and gastric cancer in this popul...AIM: TO evaluate the accuracy of two non-invasive tests in a population of Alaska Native persons. High rates of Helicobacter pylori (H. pylon] infection, H. pyIori treatment failure, and gastric cancer in this population necessitate documentation of infection status at multiple time points over a patient's life.urease test, 13C urea breath test (UBT), and immunoglobulin G antibodies to H. pylori in serum. The performances of 13C-UBT and antibody test were compared to a gold standard defined by a positive H. pylori test by culture or, in case of a negative culture result, by positive histology and a positive rapid urease test.RESULTS: The sensitivity and specificity of the 13C- UBT were 93% and 88%, respectively, relative to the gold standard. The antibody test had an equivalent sensitivity of 93% with a reduced specificity of 68%. The false positive results for the antibody test were associated with previous treatment for an H. pylorlinfection [relative risk (RR) = 2.8]. High levels of antibodies to H. py/or/were associated with chronic gastritis and male gender, while high scores in the 13C-UBT test were associated with older age and with theH. pylori bacteria load on histological examination (RR = 4.4). CONCLUSION: The 13C-UBT outperformed the antibody test for H. pylori and could be used when a noninvasive test is clinically necessary to document treatment outcome or when monitoring for reinfection.展开更多
基金Supported by General Program of Science and Technology Plan of Beijing Municipal Commission of Educational(KM201410020007)~~
文摘[Objective] This study aimed to establish a method for determining the content of Astragaloside IV in Yupingfeng oral solution.[Method] The HPLC-ELSD method was adopted.The chromatographic column was Venusil MP(4.6 mm × 150 mm,5 μm).The mobile phase was acetonitrile-water(35∶65).The ELSD evaporator tube temperature was 65 ℃.N2 was used as the carrier gas(pressure,30 psi).[Result] When the content of Astragaloside IV ranged from 0.5 to 5.0 μg,the Astragaloside IV content showed a good linear relationship with peak area(r=0.999,n=6).The average recovery was 96.36%,and the RSD was 2.46%.[Conclusion] This method is accurate and reliable,and can be applied in the quality control of Yupingfeng oral solution.
基金Under the auspices of National Natural Science Foundation of China(No.41501090,41501105)Fundamental Research Funds for Central Universities(No.2412015KJ023)
文摘In order to study the diurnal variation of soil CO2 effiux from temperate meadow steppes in Northeast China, and determine the best time for observation, a field experiment was conducted with a LI-6400 soil CO2 flux system under five typical plant communi- ties (Suaeda glauca (Sg), Chloris virgata (Cv), Puecinellia distans (Pd), Leymus chinensis (Lc) and Phragmites australis (Pa)) and an alkali-spot land (As) at the meadow steppe of western Songnen Plain. The results showed that the diurnal variation of soil CO2 effiux exhibited a single peak curve in the growing season. Diurnal maximum soil respiration (Rs) often appeared between 1 1:00 and 13:00, while the minimum occurred at 21:00-23:00 or before dawn. Air temperature near the soil surface (Ta) and soil temperature at 10 cm depth (Tlo) exerted dominant control on the diurnal variations of soil respiration. The time-windows 7:00-9:00 could be used as the optimal measuring time to represent the daily mean soil CO2 effiux at the Cv, Pd, Lc and Pa sites. The daily mean soil CO2 effiux was close to the soil COz effiux from 15:00 to 17:00 and the mean of 2 individual soil CO2 effiux from 15:00 to 19:00 at the As and Sg sites, respectively. During nocturnal hours, negative soil CO2 fluxes (CO2 downwards into the soil) were frequently observed at the As and Sg sites, the magnitude of the negative CO2 fluxes were 0.10-1.55 gmol/(m2.s) and 0.10-0.69 gmol/(m2.s)at the two sites. The results im- plied that alkaline soils could absorb CO2 under natural condition, which might have significant implications to the global carbon budget accounting.
基金Project(71101109) supported by the National Natural Science Foundation of China
文摘Traffic flow prediction is an important component for real-time traffic-adaptive signal control in urban arterial networks.By exploring available detector and signal controller information from neighboring intersections,a dynamic data-driven flow prediction model was developed.The model consists of two prediction components based on the signal states(red or green) for each movement at an upstream intersection.The characteristics of each signal state were carefully examined and the corresponding travel time from the upstream intersection to the approach in question at the downstream intersection was predicted.With an online turning proportion estimation method,along with the predicted travel times,the anticipated vehicle arrivals can be forecasted at the downstream intersection.The model performance was tested at a set of two signalized intersections located in the city of Gainesville,Florida,USA,using the CORSIM microscopic simulation package.Analysis results show that the model agrees well with empirical arrival data measured at 10 s intervals within an acceptable range of 10%-20%,and show a normal distribution.It is reasonably believed that the model has potential applicability for use in truly proactive real-time traffic adaptive signal control systems.
基金supported by the National Natural Science Foundation of China(No. 51975293)the Aeronautical Science Foundation of China (No. 2019ZD052010)。
文摘The visual inspection is an economical and effective method for welding. For measuring the feature sizes of grooves,a method based on line structured light is presented. Firstly,an adaptive algorithm to extract the subpixel centerline of structured light stripes is introduced to deal with the uneven width and grayscale distributions of laser stripes,which is based on the quadratic weighted grayscale centroid. By means of region-of-interest(ROI)division and image difference,an image preprocessing algorithm is developed for filtering noise and improving image quality. Furthermore,to acquire geometrical dimensions of various grooves and groove types precisely,the subpixel feature point extraction algorithm of grooves is designed. Finally, experimental results of feature size measuring show that the absolute error of measurement is 0.031—0.176 mm,and the relative error of measurement is 0.2%—3.6%.
基金National Natural Science Foundation of China(No.519705449)。
文摘To automatically detecting whether a person is wearing mask properly,we propose a face mask detection algorithm based on hue-saturation-value(HSV)+histogram of oriented gradient(HOG)features and support vector machines(SVM).Firstly,human face and five feature points are detected with RetinaFace face detection algorithm.The feature points are used to locate to mouth and nose region,and HSV+HOG features of this region are extracted and input to SVM for training to realize detection of wearing masks or not.Secondly,RetinaFace is used to locate to nasal tip area of face,and YCrCb elliptical skin tone model is used to detect the exposure of skin in the nasal tip area,and the optimal classification threshold can be found to determine whether the wear is properly according to experimental results.Experiments show that the accuracy of detecting whether mask is worn can reach 97.9%,and the accuracy of detecting whether mask is worn correctly can reach 87.55%,which verifies the feasibility of the algorithm.
基金Project(20235020) supported by the National Natural Science Foundation of China
文摘The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), alternative moving window factor analysis (AMWFA) algorithms and normalization method based on the peak areas; the flavones in the barks and leaves of Eucommia ulmoides Oliver were separated on an ODS column by gradient elution carried out with the flow phase consisting of water, methanol and phosphoric acid (0.1%), and their contents were quantitatively determined by standard curve method and diode array detection (DAD) at 362 nm. The results show that 68 and 73 compounds respectively from essential oils of the barks and leaves of Eucommia ulmoides Oliver are identified, and there are 33 mutual compounds among 108 compounds determined. The total contents of these volatile components of the two samples possess 92.9% and 97.75% of the gross of the relevant essential oils, respectively; the contents of the rutin, quercetin and kaempferol in the barks and leaves of Eucommia ulmoides Oliver are 0.016 9, 0.003 6, 0.002 1 and 0.064 4, 0.030 2, 0.010 0 mg/g, respectively, and the determination recoveries are 95.2%-106.2%. The comparative analysis shows that for the barks and leaves of Eucommia ulmoides Oliver, there are significant differences in their components of the relevant essential oils and flavones.
基金supported by the Fundamental Research Funds for the Central Universities(No.YX2010-20)the Open Projects Foundation of Key Laboratory of Soil and Water Conservation & Desertification Combat (Beijing ForestryUniversity), Ministry of Education of P.R. China (No.201002) the National Natural Science Foundation of China (No. 31570708, No.30901162)
文摘It is important to quantify the effect of the root diameter, the embedment length of the root and load speed on the soil-root interface mechanical properties for studying the root anchorage. The soilroot interface mechanical properties can be obtained through the pullout force and root slippage curve(F-S curve). About 120 Pinus tabulaeformis single roots whose diameters ranged from 1 mm to 10 mm divided into 6 groups based on different root embedment length(50 mm, 100 mm and 150 mm) and different load velocity(10 mm·min^(-1), 50 mm·min^(-1), 100 mm·min^(-1) and 300 mm·min^(-1)) were investigated using the pullout method. This study aims to explore the mechanical properties of the soil-root interface in the real conditions using the pullout test method. The results showed two kinds of pullout test failure modes during the experimental process: breakage failure and pullout failure. The results showed that the roots were easier to be broken when the root diameter was smaller or the loading speed was larger. The relationship between the maximum anchorage force and root diameter was linear and the linearly dependent coefficient(R^2) was larger than 0.85. The anchorage force increased with the root embedment length. An increase of 10%^(-1)5% for the maximumanchorage force was found when load speed increased from 10 to 300 mm.min^(-1). The mean peak slippage of the root was from 13.81 to 35.79 mm when the load velocity varied from 10 to 300 mm.min^(-1). The study will be helpful for the design of slopes reinforced by vegetation and in predicting risk of uprooting of trees, and will have practical benefits for understanding the mechanism of landslide.
基金Supported by Research Award (2005c24001) from Department of Science and Technology, Zhejiang Province, China
文摘AIM: To characterize changes in ghrelin levels in response to oral glucose tolerance test (OGTT) and to correlate changes in ghrelin levels with changes in insulin and glucose following OGTT in Chinese obese children of Tanner Ⅰ and Ⅱ stage with insulin resistance. METHODS: 22 obese children with insulin resistance state were divided into four groups according to their Tanner stage and gender: boys of Tanner Ⅰ (fir- Ⅰ ), boys of Tanner Ⅱ(BT-Ⅱ ), girls of Tanner Ⅰ (GT- Ⅰ ), girls of Tanner Ⅱ (GT-Ⅱ). Ghrelin, insulin and glucose were measured at 0, 30, 60 and 120 rain following OGTT. The control children with normal BMI were divided into control boys of Tanner Ⅰ (CBT- Ⅰ, n = 6), control boys of Tanner Ⅱ (CBT-Ⅱ, n = 5), control girls of Tanner Ⅰ (CGT- Ⅰ, n = 6), control girls of Tanner Ⅱ (CGT-Ⅱ, n = 5). Fasting serum ghrelin levels were analyzed. RESULTS: Ghrelin levels were lower in obese groups. Ghrelin levels of control group decreased in Tanner Ⅱ stage (CGT- Ⅰ vs CGT-Ⅱ t = -4.703, P = 0.001; CBT- Ⅰ vs CBT- Ⅱ t = -4.794, P = 0.001). Basal ghrelin levels in fir-Ⅱ decreased more significantly than that in BT- Ⅰ group (t = 2.547, P = 0.029). Ghrelin levels expressed a downward trend after OGTT among obese children. The decrease in ghrelin levels at 60 min with respect to basal values was 56.9% in BT- Ⅰ. Ghrelin concentrations at 0 min correlated directly with glucose level at 0 min in fir- Ⅰ (r = 0.898, P = 0.015). There wasn't a significant correlation of ghrelin changes with glucose changes and insulin changes during OGTT in obese children with insulin resistance. CONCLUSION: In conclusion, in obese children with insulin resistance, ghrelin levels decreased with advancing pubertal stage. Ghrelin secretion suppression following OGTT was influenced by gender and pubertal stage. Baseline ghrelin levels and ghrelin suppression after OGTT did not significantly correlate with the degree of insulin resistance and insulin sensitivity.
基金Supported by The Centers for Disease Control and Prevention13C urea breath tests were provided by Meretek Diagnostics Inc.,Lafayette,CO,United States
文摘AIM: TO evaluate the accuracy of two non-invasive tests in a population of Alaska Native persons. High rates of Helicobacter pylori (H. pylon] infection, H. pyIori treatment failure, and gastric cancer in this population necessitate documentation of infection status at multiple time points over a patient's life.urease test, 13C urea breath test (UBT), and immunoglobulin G antibodies to H. pylori in serum. The performances of 13C-UBT and antibody test were compared to a gold standard defined by a positive H. pylori test by culture or, in case of a negative culture result, by positive histology and a positive rapid urease test.RESULTS: The sensitivity and specificity of the 13C- UBT were 93% and 88%, respectively, relative to the gold standard. The antibody test had an equivalent sensitivity of 93% with a reduced specificity of 68%. The false positive results for the antibody test were associated with previous treatment for an H. pylorlinfection [relative risk (RR) = 2.8]. High levels of antibodies to H. py/or/were associated with chronic gastritis and male gender, while high scores in the 13C-UBT test were associated with older age and with theH. pylori bacteria load on histological examination (RR = 4.4). CONCLUSION: The 13C-UBT outperformed the antibody test for H. pylori and could be used when a noninvasive test is clinically necessary to document treatment outcome or when monitoring for reinfection.