AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological salin...AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological saline solution (05 ml) was ip injecte d into mice in control group The dose of MTX (05 ml) was ip injected int o mic e in other groups only once During 10 consecutive days after administration of MTX,3 mice were sacrificed in each day in all the groups and the patholog ical chang es of oral mucosa were examed under the optical microscope RE SULT: The incidence(45%～63%) and the integral rate(194%～56%) of mice ora l mu cosa lesions in group of intermediate-dose MTX (IDMTX) (20～40 mg/kg) were sign ifi cantly higher than those in other groups The death rate (not more than 5%) in group of IDMTX was lower than that in other groups CONCLUSION:As a result of induction of higher lesion rate and lower death rate of mice oral mucosa by IDMTX (2 0～40 mg/kg),the model of IDMTX-induced oral mucosa lesion could be applie d into clinical or basic展开更多
背景:目前国内外脊髓损伤模型的设计种类繁多,各有其优缺点,但尚未有一种客观化、标准化的模型以满足临床研究。目的:设计一种简易打击器并建立脊髓损伤模型,通过与NYU(New York University)打击器建立的脊髓损伤模型进行对比,评估自制...背景:目前国内外脊髓损伤模型的设计种类繁多,各有其优缺点,但尚未有一种客观化、标准化的模型以满足临床研究。目的:设计一种简易打击器并建立脊髓损伤模型,通过与NYU(New York University)打击器建立的脊髓损伤模型进行对比,评估自制改良Allen’s脊髓损伤模型的参数及稳定性。方法:雌性SD大鼠随机分为5组,即假手术组(A组6只),自制打击器5,10 cm组(B1,B2组),NYU打击器1.25,2.5 cm组(C1,C2组),A组不打击,其余各组根据分组的不同高度进行打击,每组6只;各组分别与造模术后1,3,5,7 d进行行为学评分,冰冻切片后用尼氏染色法观察病理学改变,并进行半定量分析。结果与结论:(1)在行为学评分、脊髓损伤面积比和脊髓前、后角神经元半定量分析中,同一时间点,其他4组与A组比较差异均有显著性意义(P<0.05),B1组和C1组、B2组和C2组差异无显著性意义(P>0.05),B1组和B2组、C1组和C2组差异均有显著性意义(P<0.05);(2)病理学改变:A组见脊髓灰、白质分界清晰,呈蝴蝶状,神经元细胞核大、核仁明显、数量较多,胞质内斑块状或虎斑样尼氏体清晰;余各组出现不同程度的淤血灶,神经元数目减少,损伤处可见细胞肿胀,轴突脱髓鞘改变,部分细胞呈空泡样改变,尼氏体模糊或消失;(3)结果证实,自制打击器能制备出不同损伤程度的脊髓损伤模型,与NYU脊髓损伤模型效果相近,可靠性高,稳定性好,且操作简单,易于推广,可为大鼠脊髓损伤的研究提供基础。展开更多
文摘AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological saline solution (05 ml) was ip injecte d into mice in control group The dose of MTX (05 ml) was ip injected int o mic e in other groups only once During 10 consecutive days after administration of MTX,3 mice were sacrificed in each day in all the groups and the patholog ical chang es of oral mucosa were examed under the optical microscope RE SULT: The incidence(45%～63%) and the integral rate(194%～56%) of mice ora l mu cosa lesions in group of intermediate-dose MTX (IDMTX) (20～40 mg/kg) were sign ifi cantly higher than those in other groups The death rate (not more than 5%) in group of IDMTX was lower than that in other groups CONCLUSION:As a result of induction of higher lesion rate and lower death rate of mice oral mucosa by IDMTX (2 0～40 mg/kg),the model of IDMTX-induced oral mucosa lesion could be applie d into clinical or basic
