目的研究口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)在人结肠癌组织中的表达变化并探讨其对结肠癌细胞增殖能力、细胞周期及早期凋亡的影响。方法收集人结肠癌及癌旁组织标本,分别以qRT-PCR及Western blot检测ORA...目的研究口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)在人结肠癌组织中的表达变化并探讨其对结肠癌细胞增殖能力、细胞周期及早期凋亡的影响。方法收集人结肠癌及癌旁组织标本,分别以qRT-PCR及Western blot检测ORAOV1在mRNA及蛋白水平的表达变化情况;采用RNAi技术获得ORAOV1低表达的Lo Vo及SW480细胞株,并使用Western blot对干扰结果进行鉴定;采用CCK-8检测干扰ORAOV1表达后两种结肠癌细胞增殖能力的变化;并采用流式细胞术检测干扰ORAOV1表达对结肠癌细胞周期及早期凋亡的影响。结果 qRT-PCR及Western blot结果分别显示结肠癌组织中的ORAOV1 mRNA及蛋白水平高于癌旁组织(P<0.05);Western blot检测证实Lo Vo及SW480细胞中ORAOV1干扰成功;在这两种结肠癌细胞株中,CCK-8检测结果显示,干扰ORAOV1可抑制细胞增殖能力;流式细胞术结果显示,干扰ORAOV1可使处于S期比例增加(P<0.05)、早期凋亡比例增加(P<0.05)。结论结肠癌组织中的ORAOV1表达增加,干扰ORAOV1可降低结肠癌细胞的增殖能力,可能与S期阻滞及细胞早期凋亡增加有关。展开更多
目的探讨 ERCC1基因过度表达与宫颈癌铂类药物化疗耐药之间的关系。方法采集宫颈癌患者术后标本,分为化疗敏感及耐药组。采用 RT-PCR 及 Western Blot 分别从核酸及蛋白水平检测 ERCC1基因的表达。结果与化疗敏感患者相比,耐药患者 ERCC...目的探讨 ERCC1基因过度表达与宫颈癌铂类药物化疗耐药之间的关系。方法采集宫颈癌患者术后标本,分为化疗敏感及耐药组。采用 RT-PCR 及 Western Blot 分别从核酸及蛋白水平检测 ERCC1基因的表达。结果与化疗敏感患者相比,耐药患者 ERCC1基因 RNA 及蛋白表达水平明显升高。结论 ERCC1基因的异常表达,可能是宫颈癌铂类药物化疗耐药的原因之一。展开更多
目的:检测乳腺浸润性乳腺癌(infiltrating breast carcinoma,IBC)中口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)和血管生成抑制因子1(vasohibin1,VASH1)的表达情况及其相互之间的关系,并分析它们在IBC中的临床意义...目的:检测乳腺浸润性乳腺癌(infiltrating breast carcinoma,IBC)中口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)和血管生成抑制因子1(vasohibin1,VASH1)的表达情况及其相互之间的关系,并分析它们在IBC中的临床意义。方法:通过免疫组织化学方法检测239例IBC和100例相应的正常乳腺组织中ORAOV1和VASH1蛋白的阳性表达情况。结果:在IBC组织中,ORAOV1和VASH1蛋白的阳性率分别为58.2%和56.9%;对照组中,ORAOV1和VASH1蛋白的阳性率分别为13.0%和12.0%,其表达差异在2组间均有统计学意义(P<0.01)。ORAOV1和VASH1蛋白阳性率的差异在IBC病人肿瘤组织的不同分化程度之间、淋巴结转移与否之间、肿瘤大小之间及不同TNM分期之间差异均有统计学意义(P<0.05~P<0.01);且ORAOV1蛋白的阳性率与VASH1蛋白的阳性率之间呈正相关关系(P<0.01)。在IBC病人中ORAOV1和VASH1蛋白阳性组术后的生存时间明显低于阴性组(P<0.05~P<0.01)。多因素回归分析显示阳性表达的ORAOV1和VASH1蛋白、肿瘤组织的大小及TNM分期高低均是影响IBC病人术后生存时间的独立预后因子(P<0.05~P<0.01)。结论:表达异常增高的ORAOV1和VASH1可能参与IBC的发生、发展,并促进其浸润和转移;在IBC病人肿瘤组织中联合检测ORAOV1和VASH1蛋白的表达情况对预测其发展和预后均有重要意义。展开更多
AIM: To identify the expression of Caspase-l(interleukin1.β converting enzyme) and its role in adenoma of the pancreas and chronic pancreatitis.METHODS: The expression of Caspase-1 was assessed in 42 pancreatic cance...AIM: To identify the expression of Caspase-l(interleukin1.β converting enzyme) and its role in adenoma of the pancreas and chronic pancreatitis.METHODS: The expression of Caspase-1 was assessed in 42 pancreatic cancer tissue samples, 38 chronic pancreatitis specimens, and 9 normal pancreatic tissues by immunohistochemistry and Western blot analysis.RESULTS: Overexpression of Caspase-1 was observed in both disorders, but there were differences in the expression patterns in distinct morphologic compartments. Pancreatic cancer tissues showed a clear cytoplasmatic overexpression of Caspase-1 in tumor cells of 71% of the tumors, whereas normal pancreatic tissues showed only occasional immunoreactivity. In chronic pancreatitis, overexpression of Caspase-1 was found in atrophic acinar cells (89 %),hyperplastic ducts (87 %), and dedifferentiating acinar cells (84 %). Although in atrophic cells a clear nuclear expression was found, hyperplastic ducts and dedifferentiating acinar cells showed dear cytoplasmic expression. Western blot analysis revealed a marked expression of the 45 kDa precursor of Caspase-1 in pancreatic cancer and chronic pancreatitis (80 %and 86 %, respectively). Clear bands at 30 kDa, which suggested the p10-p20 heterodimer of active Caspase-1, were found in 60 % of the cancer tissue and 14 % of the pancreatitis tissue specimens, but not in normal pancreatic tissues.CONCLUSION: Overexpression of Caspase-1 is a frequent event in pancreatic disorders and its differential expression patterns may reflect two functions of the protease. One is its participation in the apoptotic pathway in atrophic acinar cells and tumor-surrounding pancreatitis tissue, the other is its possible role in proliferative processes in pancreatic cancer cells and hyperplastic duct cells and dedifferentiating acinar cells in chronic pancreatitis.展开更多
文摘目的:检测乳腺浸润性乳腺癌(infiltrating breast carcinoma,IBC)中口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)和血管生成抑制因子1(vasohibin1,VASH1)的表达情况及其相互之间的关系,并分析它们在IBC中的临床意义。方法:通过免疫组织化学方法检测239例IBC和100例相应的正常乳腺组织中ORAOV1和VASH1蛋白的阳性表达情况。结果:在IBC组织中,ORAOV1和VASH1蛋白的阳性率分别为58.2%和56.9%;对照组中,ORAOV1和VASH1蛋白的阳性率分别为13.0%和12.0%,其表达差异在2组间均有统计学意义(P<0.01)。ORAOV1和VASH1蛋白阳性率的差异在IBC病人肿瘤组织的不同分化程度之间、淋巴结转移与否之间、肿瘤大小之间及不同TNM分期之间差异均有统计学意义(P<0.05~P<0.01);且ORAOV1蛋白的阳性率与VASH1蛋白的阳性率之间呈正相关关系(P<0.01)。在IBC病人中ORAOV1和VASH1蛋白阳性组术后的生存时间明显低于阴性组(P<0.05~P<0.01)。多因素回归分析显示阳性表达的ORAOV1和VASH1蛋白、肿瘤组织的大小及TNM分期高低均是影响IBC病人术后生存时间的独立预后因子(P<0.05~P<0.01)。结论:表达异常增高的ORAOV1和VASH1可能参与IBC的发生、发展,并促进其浸润和转移;在IBC病人肿瘤组织中联合检测ORAOV1和VASH1蛋白的表达情况对预测其发展和预后均有重要意义。
文摘AIM: To identify the expression of Caspase-l(interleukin1.β converting enzyme) and its role in adenoma of the pancreas and chronic pancreatitis.METHODS: The expression of Caspase-1 was assessed in 42 pancreatic cancer tissue samples, 38 chronic pancreatitis specimens, and 9 normal pancreatic tissues by immunohistochemistry and Western blot analysis.RESULTS: Overexpression of Caspase-1 was observed in both disorders, but there were differences in the expression patterns in distinct morphologic compartments. Pancreatic cancer tissues showed a clear cytoplasmatic overexpression of Caspase-1 in tumor cells of 71% of the tumors, whereas normal pancreatic tissues showed only occasional immunoreactivity. In chronic pancreatitis, overexpression of Caspase-1 was found in atrophic acinar cells (89 %),hyperplastic ducts (87 %), and dedifferentiating acinar cells (84 %). Although in atrophic cells a clear nuclear expression was found, hyperplastic ducts and dedifferentiating acinar cells showed dear cytoplasmic expression. Western blot analysis revealed a marked expression of the 45 kDa precursor of Caspase-1 in pancreatic cancer and chronic pancreatitis (80 %and 86 %, respectively). Clear bands at 30 kDa, which suggested the p10-p20 heterodimer of active Caspase-1, were found in 60 % of the cancer tissue and 14 % of the pancreatitis tissue specimens, but not in normal pancreatic tissues.CONCLUSION: Overexpression of Caspase-1 is a frequent event in pancreatic disorders and its differential expression patterns may reflect two functions of the protease. One is its participation in the apoptotic pathway in atrophic acinar cells and tumor-surrounding pancreatitis tissue, the other is its possible role in proliferative processes in pancreatic cancer cells and hyperplastic duct cells and dedifferentiating acinar cells in chronic pancreatitis.
