长期施肥稻田土壤胞外酶活性对底物可利用性的响应特征

土壤中碳(C)和氮(N)等底物的可利用性决定着微生物生长代谢,同时影响土壤胞外酶活性.为探讨土壤酶活性对土壤原有有机质变化的响应,本试验选取了长期定位试验田的4种施肥处理水稻土[无肥对照(CK)、单施化肥(NPK)、有机肥+化肥配施(OM)和秸秆还田+化肥配施(ST)],通过0、 4、 8和12个月的分段培养获取了具有不同可利用性C、N含量梯度的土壤,分析参与土壤碳氮转化过程的关键酶β-1,4-葡萄糖苷酶(BG)和β-1,4-N-乙酰氨基葡萄糖苷酶(NAG)活性与可溶性有机碳(DOC)、铵态氮(NH4^+-N)、土壤微生物生物量碳(MBC)和微生物生物量氮(MBN)含量的关系.结果表明,OM和ST处理对土壤中DOC含量的提高更显著(P<0.01),是CK和NPK处理的2~3倍.NPK、OM和ST处理的MBC含量、BG和NAG酶活性高于CK处理.所有施肥处理中,随着可利用性底物(DOC和NH4^+-N)含量的升高,BG和NAG活性整体呈稳定或下降趋势,MBC和MBN含量变化趋势与BG和NAG相同.施肥处理和培养时间以及二者的交互作用极显著影响(P<0.01)土壤DOC、NH4^+-N、MBC和MBN的含量.回归分析显示, OM处理MBC/MBN值与DOC/NH4^+-N值之间正相关(P<0.05);ST处理的ln(BG)/ln(NAG)值和DOC/NH4^+-N值之间负相关(P<0.01),这表明稻田土壤可利用性底物浓度是影响胞外酶活性的关键因子,且微生物量的碳氮计量比受控于土壤中底物的碳氮计量关系.该结果对深入研究稻田土壤中胞外酶活性变化规律,调节稻田土壤碳氮平衡,提高稻田土壤肥力具有一定指导意义.

Phosphorus speciation in sediments of Lake Hongfeng, China

This study was conducted to evaluate the distribution characteristics of phosphorus and its species in 14 sediments samples collected from Lake Hongfeng based on sequential extraction. Lake Hongfeng, a major drinking-water source for Guiyang City in southwestern China, is one of the largest artificial reservoirs located in a typical karstic area of the Yunnan-Guizhou Plateau. The results of this study indicate that the average percentages of DP, Al-P, Fe-P, Ca-P and OP in the lake sediments were 0.52, 6.59, 6.09, 42.85 and 40.27, respectively. The concentrations of organic phosphorus (OP) were lower than those of inorganic phosphorus (IP), which consisted mainly of calcium-bound phosphorus (Ca-P). The high concentrations of Ca-P may temporarily control the release of phosphorus from the sediments because it is a relatively stable, inert, and non-bioavailable phosphorus fraction. However, a large number of phosphate solubilizing bacteria can transform insoluble phosphate into bioavailable forms. Moreover, the concentrations of total phosphorus (TP) in the lake sediments were high; thus, the potential for the release of phosphorus from the sediments to the water column and phosphorus bioavailability were still significant. Further statistical analyses of the results revealed significant correlations between phosphorus species in sediments from the lake with two extractable principal component species (PCs) and five selectable cluster levels allowing interpretation of possible origins of phosphorus loading and the release of phosphorus. Furthermore, available remediation measures were briefly assessed for the lake with consideration of its distinctive environmental features.

Distribution of Soil Zinc，Iron，Copper and Manganese Fractions and Its Relationship with Plant Availability

The distribution of various fractions of Zn, Fe, Cu and Mn in 15 types of sods in China and its rela-tionship with plant availability were studied. Wactions of various elements were found to have some similarcharacteristic distribution regularities in wirious types of soils, but various soil types derered to varyingdegrees in the distribution of each fraction. Soil physico-chemical properties, such as pH, CEC and thecontents of OM, CaCO_3, free Fe, free Mn and P_2O_5, were signdicantly correlated with the distribution ofelemental fractions, and a significazit correlation also existed between the distribution and plant amilabilityof elemental fractions. Varfous fractions of each element were divided into two groups bed on their plantavailability. The correlation between the distribution of combination fractions aiid plaxit availability indi-cated a significantly or an extremely significantly positive correlation for Group I but a significantly or anextremely significantly negative correlation for Group II. Therefore, the fractions in Group I were primarypools of available nutrients, while those in Group II could hardly provide available nutrients for plants. Descreasing the transformation of corresponding elements into fractions of Group 11 and increasing the storagecapacity of various fractions of Group I were an important direction for regulation and controiling of soilnutrients. However, some Particular soils with too high contents of Zn, Fe, Cu and Mn should be regulatedand controlled adversely

Phosphorus Speciation in Wetland Sediments of Zhujiang (Pearl) River Estuary,China

Phosphorus fractions and adsorption-release characteristics of sediments in the Zhujiang （Pearl） River estuary wetland were investigated. Results showed that the total phosphorus （TP） content in surface sediments ranged from 648.9 mg/kg to 1064.0 mg/kg; inorganic phosphorus （IP） was the major fraction of TP and ranged from 422.5 mg/kg to 643.9 mg/kg. Among the inorganic phosphorus, the main fractions were phosphorus bound to A1 and Fe （Fe/A1-P）, and calcium-bound phosphorus （Ca-P）, accounting for 23%-42% and 21%-67% of IP, respectively. The vertical distribution of TP contents were significantly positive correlated with organic phosphorus （Org-P） and Fe/A1-P contents. The bio-available phosphorus contents in vertical sediments varied from 128.6 mg/kg to 442.9 mg/kg, mainly existed in Fe-AI/P fraction, and increased from the bottom to top sediments. The transport of phosphorus in sediment-water in- terface was controlled by the soil characteristics. The active Fe and A1 content was considered as the main factor that determines adsorp- tion capacity in vegetated marsh wetland. The P buffering capacity of the sediments in vegetated marsh wetland was greater than that in mudflat wetland. The potential risk of eutrophication in the study area is high. Reducing terrestrial phosphorus discharge and preventing the sediment Fe/A1-P release to the interstitial water are the possible solutions to reduce the risk of eutrophication in estuary wetlands, and planting vegetation in estuary wetland can also reduce the release of phosphorus in surface sediment.

Zn-Cu Interaction Affecting Zn Adsorption and Plant Availability in a Metal-Contaminated SoilA

In a previous greenhouse experiment, we showed that there was an interaction between Cu and Zn, which affected growth and metal uptake by young barley plants grown on soil to which Cd, Cu, Pb, and Zn had been added. We suggested that the underlying mechanism was the control of the amount of plant-available Zn by competitive adsorption between Cu and Zn. In order to test this hypothesis, the adsorption of Zn alone, and in the presence of added Cd, Cu and Pb, has been measured using the same soil. Following adsorption, the extractability of the Zn in CaCl2 solution was measured. The adsorption isotherms showed that of the added metals only Cu had a large effect on Zn adsorption. The effect of Cu was to reduce Zn adsorption and to increase the amount of CaCl2-extractable (i.e. plant-available) Zn, in agreement with the conclusions from the greenhouse experiment. The magnitude of the effect of Cu on plant-available Zn was similar in both experiments.

WISE EXPLOITATION OF NEWLY GROWING LAND RESOURCES——An Assessment on Land-use Change of Chongming Island Using GIS

Chongming Island, the third largest island in China and the largest alluvialisland in the world, is situated in the north of Shanghai Municipality at the mouth of theChangjiang (Yangtze) River. Along the fertile and prosperous sea coast there are a total area ofover 120 x 10~3ha, with a population of 735 000, accruing some 500ha of new tidal land resourcescome from silt, sand and mud carried by the Changjiang River every year, extending about 140m peryear. This dynamic process of alluvial growth has run for some 1500 years. Mudflat on ChongmingIsland at the mouth of the Changjiang River is a resting ground for migratory birds and host morethan a hundred species, including rare cranes and geese. But the local people keep reclaiming thetidal land for economic development. Obviously, it is crucial to have a well-concerted plan forfuture exploitation. In this study, we attempted to investigate the status changes of land use andwild life habitats on Chongming Island in recent 10 years, and then analyzed different humanactivities and their effects on wild life habitats using satellite image data (1990, 1997 and 2000)as well as field survey. Based on the analysis, this study explored the relationships between islandgrowth and land use/cover change (LUCC), predicted what the habitat would be like in the future andtried to find more effective use of this new growing resource. At last, this study provided somepreliminary management plans for Chongming Island that will coordinate the development of localeconomies and the conservation of wild life and their habitats.

Isolation and Properties Characterization of Biosurfactant Synthesized by Pyrene Degrading Bacillus subtilis C19

Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolated and identified by chromatographic and spectral analyses. Lipopeptide biosurfactant was produced and it has stable emulsification activity. Biosurfactant was produced for enhancing pyrene uptake and bioavaibility. After 30 days incubation, there were no toxic metabolite and biodegradation of pyrene was already complete （only 0.02% undegradable metabolite）. Therefore, this strain is suitable for PAHs （polyaromatic hydrocarbons） contaminated environment recovery.

Variation of Phosphorus Bioavailability in the Re-suspended Sediments

The variation of phosphorus （P） bioavailability in terms of water soluble P （WSP ）, readily desorbable P （RDP）, algal available P （AAP）, and NaHCo3 extracting P （Olsen-P） in the re-suspended sediments was investigated in laboratory experiments, in which the waters and sediments were taken from campus canal. The results indicate that sediment re-suspension can promote the migration, of soluble reactive P （SRP） from overlying water to sediments. The contents of AAP and Olsen-P in re-suspended sediments reduced obviously, whereas the values of the sediments in the control increased slightly, compared with the initial state, indicating that the P bioavailability in the sediments could be rcduced evidently due to sediment re-suspension. The content and characteristics of iron-bound P （BD-P） significantly affect the formation of AAP. The formation of OlservP has close relationship with the contents of BD-P, almninium-botmd P （AI-P）, and organic P （NaOH-nrP）.

On resource survey of natural mineral drugs in eastern Jilin and their sustaining application

There are rich natural resources of natural mineral drugs in eastern Jilin Province. Systematic resource investigation can elevate fractional conversion of this area' s mineral drugs resources superiority. Research on natural mineral drugs of this area can upgrade the translation rate of resource superiority and accelerate the development of local medical industry, especially, it can provide scientific data for founding the strategic design of Chinese traditional medicine's trademark of Jilin Changbai Mountain. Since the resource of mineral drugs can not be regenerated, it must be exploited scientifically, utilized reasonably and protected effectively its sustaining application.

Private Forest Owners Play Key Role in European Forest Derived Fuel Utilization

The AFO （activating private forest owners to increase forest fuel supply） project was set up to discover the best tools for activating an important fraction of the 12 million PFOs （private forest owners） in Europe to supply wood fuel, especially to small and medium-sized heating plants. It aimed to increase the utilization of Europe＇s vast forest fuel reserves. This was expected to benefit both the economy of the forest owners and rural areas and increase the use of renewable energy sources. The project was carried out between 2009 and 2012. The project operated in selected target regions that have a high proportion of private forest ownership （France, Slovenia, Latvia and the UK）. It started by analyzing the potential wood fuel supply and use in relation to current regional markets. After assessing through inquiries the potential wood fuel supply and demand and the parties involved, the next step was to initiate and support the formation of wood fuel supply clusters and organize supply chains. This was followed by the dissemination of best practice examples from Austria and Finland--countries with the most expertise and long traditions of forest fuel production. In the last phase of the project, results and approved activation methods were disseminated to all 27 EU （European Union） countries.

Solid Waste Generation and Composition in Mexico： A Comparison between Rural and Urban Communities

This paper reports a generation and composition analysis of household solid waste in Mexico, using as a reference the characterization study performed in two communities, one urban and one rural. Data obtained are fundamental parameters for the planning of urban solid waste management systems in Mexico, complying this way with the established policy in the National Program for Waste Management and Prevention 2009-2012. The study was carried out during five days and two main parameters were assessed： （1） the daily generation rate per person and （2） the waste composition. The per capita waste generation was 0.958 kg for the urban community and 0.631 kg for the rural. The results showed that the mean composition of waste from both locations was statistically similar （a = 0.05）. In both locations the most important contribution was the fraction of food waste. In both locations more than 60% of the waste is potentially recyclable. The results of this study will be used by the municipal waste authorities in order to establish an integrated waste management plan.

Coastal biodiversity and bioresources: variation and sustainability

The 1^(st) International Coastal Biology Congress(1^(st) ICBC) was held in Yantai, China, in Sep. 26–30, 2014. Eighteen manuscripts of the meeting presentations were selected in this special issue. According to the four themes set in the ICBC meeting, this special issue include four sections, i.e., Coastal Biodiversity under Global Change, Adaptation and Evolution to Special Environment of Coastal Zone, Sustainable Utilization of Coastal Bioresources, and Coastal Biotechnology. Recent advances in these filed are presented.

Resource Evaluation of Heavy Rare Earth Derived from the Spent Gd203 Burnable Poison in LWRs

A burnup calculation has been performed to evaluate heavy rare earth （terbium--Tb, dysprosium--Dy） production in spent gadolinium oxide （Gd203） installed as a BP （burnable poison）. Each amount of Tb and Dy production derived from the BP has been about 30-40 times larger than those created in FP （fission products）. Required cooling time to achieve exemption level on radioactivity concentration produced Tb and Dy derived from the BP are much shorter （BP-Tb： 7.9y, BP-Dy： 〈 0.1y） than those created in FP （FP-Tb： 3,616y, FP-Dy： 6.9y）. However, the BP is mixed homogeneously with UO2 pellet in current nuclear fuel system of LWRs （light water reactors）, and hence mixing of FP cannot be not avoided. In such a mixture case, the required cooling time of recovered Tb will become 2,653y and that of recovered Dy be 4.8y. For this reason, recovered Tb is unlikely to be resource for utilization, while recovered Dy must be the resource provided the precise separation from the other FP.

Tea and human health: biomedical functions of tea active components and current issues

Originating in China, tea and tea planting have spread throughout the world since the middle of the Tang dynasty. Now people from 160 countries in the world are accustomed to tea drinking. A brief history of tea＇s medicinal role in China and its spread to the world are introduced. The effectiveness of tea active components and tea drinking on major human diseases, including cancer, metabolic syndrome, cardiovascular disease, and neurodegenerative diseases, is discussed. Also presented are some related issues, such as the bioavailability of tea active components, the new formulations of tea polyphenols, and the safety for consumers of dietary supplements containing tea polyphenols.

Success of native and invasive plant congeners depends on inorganic nitrogen compositions and levels

Aims Successful plant invaders usually exhibit three strategies:Jack-of-all-trades(more robust in stressful sites),Master-of-some(more responsive in favorable sites)and Jack-and-master(both robustness and responsiveness).To revisit these strategies,we examined how soil inorganic nitrogen(N)compositions and levels influence the success of native and invasive plant congeners in the context of plant communities.Methods We conducted an experiment involving three fixed factors:species origin,N composition and N level.Here,we selected 21 plant species(eight pairs of invasive and native congeners and five non-congeneric natives)to assemble plant communities,which were subject to nine N environments consisting of three N compositions(3:1,2:2 and 1:3 NO3−/NH4+)and three N levels(low,medium and high N).We determined the following metrics:total biomass,relative biomass(a proxy of species success),mortality rate and mortality time.Important Findings Across nine N environments,native and invasive congeners exhibited similar total biomass,relative biomass and mortality time,but invaders had a marginally lower mortality rate than natives.Similar success between native and invasive congeners was linked to their similar growth and tolerance.N compositions influenced mortality time and N levels affected the total biomass and relative biomass.Importantly,species origin,N composition and N level interactively affected the total biomass,relative biomass and mortality time.These findings suggest that native and invasive plant congeners may be similarly successful across different N environments,and that inorganic N compositions and levels both contribute to plant invasion success.

Recycling synthesis of single-atom Zn-nitrogen-carbon catalyst for electrocatalytic reduction of O_(2)to H_(2)O_(2)

Portable electrochemical synthesis of H_(2)O_(2),which is widely used for disinfection,sterilization,and waste treatment,has attracted increasing attention.However,low atomic utilization efficiency and by-product disposal during the synthesis of electrocatalysts are inevitable.Based on the advantages of single-atom catalysts(SACs)towards H_(2)O_(2)synthesis via a 2e−transfer oxygen reduction reaction,this work presents the synthesis of ZnNC SACs as excellent oxygen reduction catalysts with high atomic use efficiency and almost no generated waste by using formamide and metal powder as precursors.The ZnNC SACs exhibit H_(2)O_(2)selectivity exceeding 80%when working continuously for 10,000 s.The synthetic strategy described in this paper is intended to be used as a supplement to the synthesis of metal-nitrogen-carbon SACs with high atomic utilization efficiency and low waste generation for environmental and chemical applications.

Recyclable polymer-based nano-hydrous manganese dioxide for highly efficient Tl(I) removal from water

TI（I） in water even at a trace level is fatal to human beings and the ecosystem. Here we fabricated a new polymer-supported nanocomposite （HMO-001） for efficient TI（I） removal by encapsulating nanosized hydrous manganese dioxide （HMO） within a polystyrene cation exchanger （D-001）. The resultant HMO-001 exhibited more preferable removal of TI（I） than D-001 and IRC-748, an iminodiacetic chelating polymer, particularly in the presence of competing Ca（II） ions at greater levels in solution. Such preference was ascribed to the Donnan membrane effect caused by D-001 as well as the specific interaction between TI（I） and HMO. The adsorbed TI（I） was partially oxidized into insoluble TI（III） by HMO at acidic pH, while negligible oxidation was observed at circumneutral pH. The exhausted HMO-001 was amenable to efficient regeneration by binary NaOH-NaC10 solution for at least 10-cycle batch runs without any significant capacity loss. Fixed-bed column test of Tl（I）-contained indus- trial effluent and natural water further validated that TI（I） retention on HMO-001 resulted in a conspicuous concentration drop from 1.3 mg/L to a value lower than 0.14 mg/L （maximum concentration level for industrial effluent regulated by US EPA） and from 1-4 μg/L to a value lower than 0.1 μg/L （drinking water standard regulated by China Health Ministry）, respectively.