使用2216E平板涂布法从肠道样品分离可培养细菌,通过16S r DNA测序鉴定细菌。所有分离的细菌分为变形菌门(包含γ-变形细菌纲和α-变形细菌纲)和厚壁菌门。其中以γ-变形细菌纲为优势(83.3%)。在属的水平,从肠道中共分离出弧菌属、希瓦...使用2216E平板涂布法从肠道样品分离可培养细菌,通过16S r DNA测序鉴定细菌。所有分离的细菌分为变形菌门(包含γ-变形细菌纲和α-变形细菌纲)和厚壁菌门。其中以γ-变形细菌纲为优势(83.3%)。在属的水平,从肠道中共分离出弧菌属、希瓦氏菌属、假交替单胞菌属、亚硫酸杆菌属、芽孢杆菌属、Aliivibrio、发光杆菌属、科尔韦尔氏菌属8个属,其中弧菌属、希瓦氏菌属和假交替单胞菌属的种类占总数的70%。首次发现亚硫酸杆菌属和科尔韦尔氏菌属作为红鳍东方鲀稚鱼肠道菌群的一部分。展开更多
Five different sites with a soluble salt gradient of 3.0-17.7 g kg^-1 dry soil from the coast to the inland were selected, and the microbial population size, activity and diversity in the rhizospheres of five common p...Five different sites with a soluble salt gradient of 3.0-17.7 g kg^-1 dry soil from the coast to the inland were selected, and the microbial population size, activity and diversity in the rhizospheres of five common plant species and the adjacent bulk soils （non-rhizosphere） were compared in a degraded wetland of the Yellow River Delta, Shandong Province, China to study the effects of soil environment （salinity, seasonality, depth, and rhizosphere） on microbial communities and the wetland＇s ecological function, thus providing basic data for the bioremediation of degraded wetlands. There was a significant negative linear relationship between the salinity and the total number of microorganisms, overall microbial activity, or culturable microbial diversity. Salinity adversely affected the microbial community, and higher salinity levels resulted in smaller and less active microbial communities. Seasonal changes were observed in microbial activity but did not occur in the size and diversity. The microbial size, activity and diversity decreased with increasing soil depth. The size, activity and diversity of culturable microorganisms increased in the rhizospheres. All rhizospheres had positive effects on the microbial communities, and common seepweed had the highest rhizosphere effect. Three halophilic bacteria （Pseudomonas mendocina, Burkholderia glumae, and Acinetobacter johnsonii） were separated through BIOLOG identification, and common seepweed could be recommended for bioremediation of degraded wetlands in the Yellow River Delta.展开更多
文摘使用2216E平板涂布法从肠道样品分离可培养细菌,通过16S r DNA测序鉴定细菌。所有分离的细菌分为变形菌门(包含γ-变形细菌纲和α-变形细菌纲)和厚壁菌门。其中以γ-变形细菌纲为优势(83.3%)。在属的水平,从肠道中共分离出弧菌属、希瓦氏菌属、假交替单胞菌属、亚硫酸杆菌属、芽孢杆菌属、Aliivibrio、发光杆菌属、科尔韦尔氏菌属8个属,其中弧菌属、希瓦氏菌属和假交替单胞菌属的种类占总数的70%。首次发现亚硫酸杆菌属和科尔韦尔氏菌属作为红鳍东方鲀稚鱼肠道菌群的一部分。
基金Supported by the National Natural Science Foundation of China (No.30570340)the National Key Technology R&DProgram of China (No.2006BAC01A13)+1 种基金the Program for New Century Excellent Talents in University, China (NCET-06-0599)the Cheung Kong Scholar Program of the Education Ministry of China
文摘Five different sites with a soluble salt gradient of 3.0-17.7 g kg^-1 dry soil from the coast to the inland were selected, and the microbial population size, activity and diversity in the rhizospheres of five common plant species and the adjacent bulk soils （non-rhizosphere） were compared in a degraded wetland of the Yellow River Delta, Shandong Province, China to study the effects of soil environment （salinity, seasonality, depth, and rhizosphere） on microbial communities and the wetland＇s ecological function, thus providing basic data for the bioremediation of degraded wetlands. There was a significant negative linear relationship between the salinity and the total number of microorganisms, overall microbial activity, or culturable microbial diversity. Salinity adversely affected the microbial community, and higher salinity levels resulted in smaller and less active microbial communities. Seasonal changes were observed in microbial activity but did not occur in the size and diversity. The microbial size, activity and diversity decreased with increasing soil depth. The size, activity and diversity of culturable microorganisms increased in the rhizospheres. All rhizospheres had positive effects on the microbial communities, and common seepweed had the highest rhizosphere effect. Three halophilic bacteria （Pseudomonas mendocina, Burkholderia glumae, and Acinetobacter johnsonii） were separated through BIOLOG identification, and common seepweed could be recommended for bioremediation of degraded wetlands in the Yellow River Delta.
