角膜可溶性抗原诱导前房相关性免疫偏离的实验研究

①目的 建立兔前房相关性免疫偏离 (ACAID)诱导模型。②方法 采用甘氨酸法制备可溶性角膜分类抗原 ,分组行兔前房注射诱导ACAID ,并观察各组耳膨胀试验和脾脏病理学检查结果。③结果 角膜上皮、内皮、基质及全角膜可溶性抗原皆可诱导ACAID ,诱导质量浓度为 2 .0 g/L .成功率分别为 90 % ,10 0 % ,80 %和 10 0 % .④结论 角膜可溶性抗原可以诱导产生ACAID .

青光眼视神经保护的免疫学途径

导致青光眼视功能损害的病理基础是视网膜神经节细胞进行性凋亡和丧失。目前对视网膜神经节细胞损伤和保护的治疗研究已取得一些进展,如:采用可溶性抗原诱导免疫耐受以避免对破坏性自身抗原应答的诱导;在避免自身免疫性疾病基础上,发挥T细胞神经保护性免疫反应的治疗作用;利用谷氨酸受体拮抗剂、钙离子通道阻滞剂、免疫抑制剂等非特异性治疗,减轻和预防视网膜神经节细胞的不可逆性损伤。本文就视网膜神经节细胞的凋亡、青光眼视神经损害的免疫学机制及视神经保护的免疫学途径进行综述,并从自身抗原、抗原多肽、免疫抑制剂等方面探讨视神经保护在免疫学的可能途径。

In vitro Induction of primary antibody responses to particulate and soluble protein antigens in T cell-replaced murine spleen cell cultures

Specific antibody responses could be induced in serumfree condition.Specific anti-SRBC or anti-SRBC ghost antibody were induced from anti-Thy treated (T-depleted) murine spleen cells in serum-free culture in the presence of Con A conditioned medium.This induction system may facilitate the study of lymphokine functions on antigen triggered B cells. In T cell-replaced cultures,the antibody responses of B cells could be successfully induced when soluble SRBC membrane proteins were used as antigens.It thus indicates that antigen together with lymphokines are sufficient to drive B cells to become antibody secreting cells in the absence of T cells.The T cell-replaced system provides a more stable way for in vitro immunization and may be applied to monoclonal antibody production when in vivo immunization is difficult to be carried out.