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刺果番荔枝叶片提取物的体外抑菌研究 被引量:1
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作者 吴志华 王伟 +1 位作者 陈晓芳 周凤芝 《浙江中医杂志》 2013年第8期607-608,共2页
目的:研究刺果番荔枝叶片的甲醇、石油醚和水可溶性提取物的抑菌效果。方法:采用肉汤稀释法对3种革兰氏阳性菌(枯草芽孢杆菌、金黄色酿脓葡萄球菌、表皮葡萄球菌)和5种革兰氏阴性菌(大肠杆菌、铜绿假单胞菌、伤寒沙门氏菌、溶藻弧菌和... 目的:研究刺果番荔枝叶片的甲醇、石油醚和水可溶性提取物的抑菌效果。方法:采用肉汤稀释法对3种革兰氏阳性菌(枯草芽孢杆菌、金黄色酿脓葡萄球菌、表皮葡萄球菌)和5种革兰氏阴性菌(大肠杆菌、铜绿假单胞菌、伤寒沙门氏菌、溶藻弧菌和霍乱弧菌)进行了体外抑菌效果试验。结果:刺果番荔枝的甲醇提取物对细菌的抑制性最强,其次是石油醚提取物和水提取物。试验中枯草芽孢杆菌,表皮葡萄球菌,金黄色酿脓葡萄球菌和溶藻弧菌对3种提取物最为敏感。结论:刺果番荔枝叶片提取物对革兰氏阳性菌和革兰氏阴性菌都有一定的抑菌效果。 展开更多
关键词 刺果番荔枝 可溶性提取物 抑菌效果
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五疫神康新型中药提取物可溶性粉
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作者 周志敏 《兽药市场指南》 2005年第3期14-14,共1页
五疫神康是一种由中药提取物组成的新型制剂,主要特点是利用中药提取的特殊工艺使药物中的各种成分发挥高于普通中药几倍的功效,它在防治畜禽疾病过程中对抗病原体、调节机体免疫功能、诱生干扰素、对抗毒素等多方面起作用,是治疗病... 五疫神康是一种由中药提取物组成的新型制剂,主要特点是利用中药提取的特殊工艺使药物中的各种成分发挥高于普通中药几倍的功效,它在防治畜禽疾病过程中对抗病原体、调节机体免疫功能、诱生干扰素、对抗毒素等多方面起作用,是治疗病毒性疾病的理想药物。 展开更多
关键词 五疫神康 中药提取可溶性 成分 作用机理 兽药
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药物制剂信息文摘
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《辽宁医药》 2005年第1期54-58,共5页
可溶性银杏叶提取物制剂;从植物中提取活性成分;肝细胞靶向化合物;一种头孢菌素的缓控释制剂处方;微观排列药物转运包衣法;生物可降解微囊的制备;
关键词 制剂 可溶性银杏叶提取制剂 活性成分 肝细胞 靶向化合 头孢菌素
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Strain and Culture Medium Optimization for Production Enhancement of Prodiginines from Marine-Derived Streptomyces sp. GQQ-10
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作者 LI Xueping ZHANG Guojian +2 位作者 ZHU Tianjiao LI Dehai GU Qianqun 《Journal of Ocean University of China》 SCIE CAS 2012年第3期361-365,共5页
A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employe... A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employed for medium optimization. In the single factor method, the effects of soluble starch, glucose, soybean flour, yeast extract and sodium acetate on PGs production were investigated individually. In the subsequent OAD experiments, the concentrations of these 5 key nutritional components combined with salinity were further adjusted. The mutant strain GQQ-M6 gave a 2.2-fold higher PGs production than that of the parent strain; OAD experiments offered a PGs yield of 61mg L-1, which was 10 times higher than that of the initial GQQ-10 strain under the original cultivation mode. 展开更多
关键词 prodiginines marine-derived streptomycete optimization culture media mutation
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A Comparative Study of Soluble Protein Extractions of Populus deltoides × ( Trichocarpa × Deltoides) for 2-DE
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作者 Joke Dupae Ann Cuypers +4 位作者 Jean-Paul Noben Jana Boulet Nele Weyens Karen Verstraelen Jaco Vangronsveld 《Journal of Life Sciences》 2012年第9期970-979,共10页
Background: The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on ... Background: The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on the extraction method of soluble proteins for this species. The extraction is the most critical step in two-dimensional gel electrophoresis and each extraction method has its advantages, disadvantages and limitations. Therefore protein extraction methods should be optimized for each tissue before starting an experimental setup. In prospect of future DIGE (Differential Gel electrophoresis) experiments for the investigation of the effects of cadmium and inoculation with plant growth promoting bacteria at the proteome level, the aim of this study was to optimize an extraction method for soluble proteins of poplar leaves and roots. Results: The acetone-phenol extraction method was found to be the most suited, rendering a high spot number and low background interference. During further optimization, several critical steps in the extraction method were revealed. Conclusion: Aiming to optimize the extraction of soluble leaf and root proteins of Populus deltoides × (trichocarpa× deltoides) compatible with DIGE analysis, a protocol rendering high reproducibility, low background interference and a high spot number was established, however no novel insights were acquired. 展开更多
关键词 PROTEOMICS two-dimensional gel electrophoresis soluble proteins protein extraction Populus.
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