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重金属抗性解磷细菌的磷溶解特性研究 被引量:12
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作者 田江 彭霞薇 +3 位作者 李霞 孙雅君 冯红梅 江泽平 《环境科学》 EI CAS CSCD 北大核心 2014年第6期2334-2340,共7页
从湖南省湘西州花垣县的铅锌矿表层土壤中,筛选出两株具有重金属抗性和解磷特性的细菌T PSB1和T PSB2.通过16S rRNA基因序列比对,分别鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)和唐菖蒲伯克霍尔德菌(Burkholderia gladioli... 从湖南省湘西州花垣县的铅锌矿表层土壤中,筛选出两株具有重金属抗性和解磷特性的细菌T PSB1和T PSB2.通过16S rRNA基因序列比对,分别鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)和唐菖蒲伯克霍尔德菌(Burkholderia gladioli).T PSB1和T PSB2在含有难溶性无机磷液体培养基中,其上清液的可溶性磷含量最高分别达到了402.9 mg·L-1和589.9 mg·L-1;在难溶性有机磷固体和液体培养基中,固体平板上均出现解磷圈,而液体培养基上清液中,可溶性磷含量也分别达到了2.97 mg·L-1和4.69 mg·L-1.另外,两株细菌对重金属Zn2+的抗性最高,在其浓度为2000 mg·L-1固体和液体培养基条件下均可以生长,磷溶解浓度分别为114.8 mg·L-1和125.1 mg·L-1.而在含铬和铅的浓度为1 000 mg·L-1的液体培养基中,两株细菌同样表现出重金属抗性.在Pb2+浓度为1000 mg·L-1的液体培养基中,磷溶解浓度分别达到了57.9 mg·L-1和71.7 mg·L-1;而在Cr2+浓度为1000 mg·L-1的培养基中磷溶解浓度分别为60.1 mg·L-1和98.4 mg·L-1. 展开更多
关键词 细菌 可溶磷量 无机 有机 重金属抗性
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Effects of Phosphate Fertilizer on Yield and Quality of Eleocharis dulcis 被引量:1
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作者 Dan ZHENG Peng GUO 《Agricultural Science & Technology》 CAS 2016年第1期81-84,共4页
The effects of different application levels and species of phosphatic fertilizer on Eleocharis dulcis were studied. The results showed that the growth stage of Eleocharis dulcis was not significantly influenced by dif... The effects of different application levels and species of phosphatic fertilizer on Eleocharis dulcis were studied. The results showed that the growth stage of Eleocharis dulcis was not significantly influenced by different levels of phosphatic fertilizer application, with phosphorus level in 195-375 kg/hm^2. With increasing of the phosphorus level, starch and total soluble sugar accumulated, but the yield was not improved significantly. The analysis on the economic benefits showed that the best phosphorus level was 195 kg/hm^2. The growth stage and yield of Eleocharis dulcis were not considerably influenced by different proportions of calcium-magnesia phosphate fertilizer and ammonium dihydrogen phosphate. With increasing of ammonium dihydrogen phosphate, big-fruit rate of Eleocharis dulcis kept growing, but the accumulations of starch and total soluble sugar were decreasing. 展开更多
关键词 Phosphatic fertilizer Application levels SPECIES Eleocharis dulcis
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Effects of temperature,algae biomass and ambient nutrient on the absorption of dissolved nitrogen and phosphate by Rhodophyte Gracilaria asiatica 被引量:2
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作者 杜荣斌 刘立明 +1 位作者 王爱敏 王勇强 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2013年第2期353-365,共13页
Gracilaria asiatica, being highly efficient in nutrient absorption, is cultivated in sea cucumber ponds to remove nutrients such as nitrogen and phosphate. It was cultured in a laboratory simulating field conditions, ... Gracilaria asiatica, being highly efficient in nutrient absorption, is cultivated in sea cucumber ponds to remove nutrients such as nitrogen and phosphate. It was cultured in a laboratory simulating field conditions, and its nutrient absorption was measured to evaluate effects of environmental conditions. Ammonia nitrogen (AN), nitrate nitrogen (NN), total inorganic nitrogen (TIN), and soluble reactive phosphorus (SRP) uptake rate and removal efficiency were determined in a 4~2 factorial design experiment in water temperatures (T) at 15~C and 25~C, algae biomass (AB) at 0.5 g/L and 1.0 g/L, total inorganic nitrogen (TIN) at 30 ~tmol/L and 60 ~tmol/L, and soluble reactive phosphorus (SRP) at 3 and 6 ~tmol/L. AB and ambient TIN or SRP levels significantly affected uptake rate and removal efficiency of AN, NN, TIN, and SRP (P〈0.001). G. asiatica in AB of 0.5 g/L showed higher uptake rate and lower removal efficiency relative to that with AB of 1.0 g/L. Nitrogen and phosphorus uptake rate rose with increasing ambient nutrient concentrations; nutrient removal efficiency decreased at higher environmental nutrient concentrations. The algae preferred to absorb AN to NN. Uptake rates of AN, NN, and SRP were significantly affected by temperature (P〈0.001); uptake rate was higher for the 25~C group than for the 15~C group at the initial experiment stage. Only the removal efficiency of AN and SRP showed a significant difference between the two temperature groups (P〈0.01). The four factors had significant interactive effects on absorption of N and P, implying that G. asiatica has great bioremedial potential in sea cucumber culture ponds. 展开更多
关键词 ALGAE Gracilaria asiatica Chang et Xia nutrient removal NITROGEN soluble reactive phosphate
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