Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the ...Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.展开更多
Objective: To study the expression of CD44 correlation with the ability ofmetastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration ofsoluble CD 44 Variant 6 (sCD44v6) and the histol...Objective: To study the expression of CD44 correlation with the ability ofmetastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration ofsoluble CD 44 Variant 6 (sCD44v6) and the histologic expression of CD44 Variant 6 (CD44v6) in tumorswith the clinico- pathologic features, and to make serum concentration of the CD44v6 and theexpression of CD44v6 may be useful an indicator as an early diagnosis, invasion, metastasis, andprognosis. Methods: Serum samples were obtained from 70 patients with primary gastric carcinomabefore surgery and 20 patients after surgery. Serum levels of CD44v6 were determined with aquantitative- enzyme- link-immuno- adsorbent assay. The expression of CD44v6 in tumors was examinedby immuno-histo-chemical staining. Results: Both the serum concentration of CD44v6 and itsexpression in tumors were positively related to the depth of invasion of the tumor, lymph nodemetastasis, clinical stage, and diffuse type gastric carcinoma, but not to the tumor size. The serumlevel of CD44v6 in patients with gastric cancer was significantly higher than that in control. Theserum concentration of CD44v6 was markedly lowered after curative surgery (F<0. 001), and the serumlevel of CD44v6 was higher in patients with gastric cancer with CD44v6 positive tumor cells than inthose with CD44v6 negative tumor cells. The serum level of CD44v6 was a prognostic indicator inpatients with diffuse type gastric carcinoma, as was the histological expression of CD44v6.Conclusion: CD44v6 of gastric cancer and serum concentration of CD44v6 seems to be correlated to theprogression of diffuse type gastric carcinoma metas- tasis and clinical stage. An elevated level ofsCD44v6 may serve as an indicator of lymph node metastasis (especially early metastasis) and badprognosis in pa tients with gastric carcinoma.展开更多
In this study, the author has designed new verifiable (t, n) threshold untraceable signature schemes. The proposed schemes have the following properties: ( 1 ) Verification: The shadows of the secret distributed by th...In this study, the author has designed new verifiable (t, n) threshold untraceable signature schemes. The proposed schemes have the following properties: ( 1 ) Verification: The shadows of the secret distributed by the trusted center can be verified by all of the participants; (2) Security: Even if the number of the dishonest member is over the value of the threshold, they cannot get the system secret parameters , such as the group secret key, and forge other member's individual signature; (3) Efficient verification: The verifier can verify the group signature easily and the verification time of the group signature is equivalent to that of an individual signature; (4) Untraceability: The signers of the group signature cannot be traced.展开更多
A novel active steering system with force and displacement coupled control(the novel AFS system) was introduced,which has functions of both the active steering and electric power steering.Based on the model of the nov...A novel active steering system with force and displacement coupled control(the novel AFS system) was introduced,which has functions of both the active steering and electric power steering.Based on the model of the novel AFS system and the vehicle three-degree of freedom system,the concept and quantitative formulas of the novel AFS system steering performance were proposed.The steering road feel and steering portability were set as the optimizing targets with the steering stability and steering portability as the constraint conditions.According to the features of constrained optimization of multi-variable function,a multi-variable genetic algorithm for the system parameter optimization was designed.The simulation results show that based on parametric optimization of the multi-objective genetic algorithm,the novel AFS system can improve the steering road feel,steering portability and steering stability,thus the optimization method can provide a theoretical basis for the design and optimization of the novel AFS system.展开更多
Based on Bloor & Ingham's approach for determining the fluid fieldand on the analyses of loci of fluid particles inside hydrocyclones,analytical models are developed for calculating the migrationprobability of...Based on Bloor & Ingham's approach for determining the fluid fieldand on the analyses of loci of fluid particles inside hydrocyclones,analytical models are developed for calculating the migrationprobability of single-cone and two-cone hydrocyclones separatinglight dispersions. The calculated results are in good agreement withThew's correlation at different flow rate, split ratio or fluidproperties if the structural parameters keep the same as those ofThew's 35 mm hydrocyclone. The difference between predictionsaccording to two-cone model and single-cone model is nearlynegligible, which is very close to thew's original idea that majorseparation happens in the small cone-angle zone.展开更多
AIM: To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and SCD95L, respectively) in plasma and CD95 expression on CD3+cells in liver-transplanted recipients with acute rejection (AR). METHOD...AIM: To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and SCD95L, respectively) in plasma and CD95 expression on CD3+cells in liver-transplanted recipients with acute rejection (AR). METHODS: Peripheral blood mohonuclear cells (PBMCs) were isolated from 30 clinically liver transplanted recipients. CD95 expression on CD3+ cells was quantitatively measured by two-color fluorescence activated cell sorter (FACS) analysis. Lymphocyte surface phenotypes of CD4, CD8, CD16 and CD56 were determined by flow cytometry. Plasma levels of sCD95 and SCD95L were detected by Enzyme Linked-Immuno-Sorbent Assay (ELISA). The results were compared with that from normal healthy volunteers (n=15 individuals). RESULTS: FACS analysis showed that CD95 expression on CD3+ T cells was significantly increased in liver transplanted recipients with AR compared to that in stable recipients without rejection and infection or healthy individuals who did not undergo transplantation (18 676.93±11 588.34/molecule, 6 848.20±1 712.96/molecule, 6 418.01±2 001.95/molecule, respectively, P<0.01). Whereas no significant difference was seen between liver-transplanted stable recipients and healthy individuals. Furthermore, no significant differences were detected between each group with CD4/CD8 ratio or the percentage of CD16+56+cells. Plasma levels of sCD95 were significantly higher in transplanted recipients with AR compared to that in stable recipients or healthy individuals (391.88±196.00, 201.37±30.30, 148.83±58.25 pg/mL, respectively, P<0.01). In contrast, the plasma levels of sCD95L in liver-transplanted recipients were not significantly different from that in healthy individuals. CONCLUSION: The present results indicate that the increased CD95 expression on CD3+cells and the increased levels of sCD95 in plasma may modify the immunological situation of the recipients after transplantation or represent the ongoing graft rejection.展开更多
基金the project of Scientific Research Foundation for the Returned Overseas Chinese Scholars, New Century Excellent Talents in University (No. NCET-04-0908)Changjiang Scholars and Innovative Research Team in University (No. IRT0 453) of the Chinese Ministry of EducationNational Natural Science Foundation of China (No. 30270804), Education Department and Science and Technology Department of Sichuan Province.
文摘Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.
基金Supported by a grant from Science and Technology Committee of Jiangsu Province(457-99064)
文摘Objective: To study the expression of CD44 correlation with the ability ofmetastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration ofsoluble CD 44 Variant 6 (sCD44v6) and the histologic expression of CD44 Variant 6 (CD44v6) in tumorswith the clinico- pathologic features, and to make serum concentration of the CD44v6 and theexpression of CD44v6 may be useful an indicator as an early diagnosis, invasion, metastasis, andprognosis. Methods: Serum samples were obtained from 70 patients with primary gastric carcinomabefore surgery and 20 patients after surgery. Serum levels of CD44v6 were determined with aquantitative- enzyme- link-immuno- adsorbent assay. The expression of CD44v6 in tumors was examinedby immuno-histo-chemical staining. Results: Both the serum concentration of CD44v6 and itsexpression in tumors were positively related to the depth of invasion of the tumor, lymph nodemetastasis, clinical stage, and diffuse type gastric carcinoma, but not to the tumor size. The serumlevel of CD44v6 in patients with gastric cancer was significantly higher than that in control. Theserum concentration of CD44v6 was markedly lowered after curative surgery (F<0. 001), and the serumlevel of CD44v6 was higher in patients with gastric cancer with CD44v6 positive tumor cells than inthose with CD44v6 negative tumor cells. The serum level of CD44v6 was a prognostic indicator inpatients with diffuse type gastric carcinoma, as was the histological expression of CD44v6.Conclusion: CD44v6 of gastric cancer and serum concentration of CD44v6 seems to be correlated to theprogression of diffuse type gastric carcinoma metas- tasis and clinical stage. An elevated level ofsCD44v6 may serve as an indicator of lymph node metastasis (especially early metastasis) and badprognosis in pa tients with gastric carcinoma.
文摘In this study, the author has designed new verifiable (t, n) threshold untraceable signature schemes. The proposed schemes have the following properties: ( 1 ) Verification: The shadows of the secret distributed by the trusted center can be verified by all of the participants; (2) Security: Even if the number of the dishonest member is over the value of the threshold, they cannot get the system secret parameters , such as the group secret key, and forge other member's individual signature; (3) Efficient verification: The verifier can verify the group signature easily and the verification time of the group signature is equivalent to that of an individual signature; (4) Untraceability: The signers of the group signature cannot be traced.
基金Project(51005115) supported by the National Natural Science Foundation of ChinaProject(KF11201) supported by the Science Fund of State Key Laboratory of Automotive Safety and Energy,ChinaProject(201105) supported by the Visiting Scholar Foundation of the State Key Laboratory of Mechanical Transmission in Chongqing University,China
文摘A novel active steering system with force and displacement coupled control(the novel AFS system) was introduced,which has functions of both the active steering and electric power steering.Based on the model of the novel AFS system and the vehicle three-degree of freedom system,the concept and quantitative formulas of the novel AFS system steering performance were proposed.The steering road feel and steering portability were set as the optimizing targets with the steering stability and steering portability as the constraint conditions.According to the features of constrained optimization of multi-variable function,a multi-variable genetic algorithm for the system parameter optimization was designed.The simulation results show that based on parametric optimization of the multi-objective genetic algorithm,the novel AFS system can improve the steering road feel,steering portability and steering stability,thus the optimization method can provide a theoretical basis for the design and optimization of the novel AFS system.
文摘Based on Bloor & Ingham's approach for determining the fluid fieldand on the analyses of loci of fluid particles inside hydrocyclones,analytical models are developed for calculating the migrationprobability of single-cone and two-cone hydrocyclones separatinglight dispersions. The calculated results are in good agreement withThew's correlation at different flow rate, split ratio or fluidproperties if the structural parameters keep the same as those ofThew's 35 mm hydrocyclone. The difference between predictionsaccording to two-cone model and single-cone model is nearlynegligible, which is very close to thew's original idea that majorseparation happens in the small cone-angle zone.
文摘AIM: To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and SCD95L, respectively) in plasma and CD95 expression on CD3+cells in liver-transplanted recipients with acute rejection (AR). METHODS: Peripheral blood mohonuclear cells (PBMCs) were isolated from 30 clinically liver transplanted recipients. CD95 expression on CD3+ cells was quantitatively measured by two-color fluorescence activated cell sorter (FACS) analysis. Lymphocyte surface phenotypes of CD4, CD8, CD16 and CD56 were determined by flow cytometry. Plasma levels of sCD95 and SCD95L were detected by Enzyme Linked-Immuno-Sorbent Assay (ELISA). The results were compared with that from normal healthy volunteers (n=15 individuals). RESULTS: FACS analysis showed that CD95 expression on CD3+ T cells was significantly increased in liver transplanted recipients with AR compared to that in stable recipients without rejection and infection or healthy individuals who did not undergo transplantation (18 676.93±11 588.34/molecule, 6 848.20±1 712.96/molecule, 6 418.01±2 001.95/molecule, respectively, P<0.01). Whereas no significant difference was seen between liver-transplanted stable recipients and healthy individuals. Furthermore, no significant differences were detected between each group with CD4/CD8 ratio or the percentage of CD16+56+cells. Plasma levels of sCD95 were significantly higher in transplanted recipients with AR compared to that in stable recipients or healthy individuals (391.88±196.00, 201.37±30.30, 148.83±58.25 pg/mL, respectively, P<0.01). In contrast, the plasma levels of sCD95L in liver-transplanted recipients were not significantly different from that in healthy individuals. CONCLUSION: The present results indicate that the increased CD95 expression on CD3+cells and the increased levels of sCD95 in plasma may modify the immunological situation of the recipients after transplantation or represent the ongoing graft rejection.
