高考读后续写评分的关注点张春青:对于写作题型的关注点,除了如何教,就是如何评价了,评价往往决定教学的方向,我们戏称为The tail wags the dog。姜建军老师和费胜昌老师有非常丰富的评分经验,下面请两位谈谈评分的主要关注点。姜建军:...高考读后续写评分的关注点张春青:对于写作题型的关注点,除了如何教,就是如何评价了,评价往往决定教学的方向,我们戏称为The tail wags the dog。姜建军老师和费胜昌老师有非常丰富的评分经验,下面请两位谈谈评分的主要关注点。姜建军:高考试卷中读后续写题总分为25分,按5个档次给分。各档次的给分范围和要求见表1。段湘萍:如何理解这其中的关键词汇呢,如融洽度高、衔接合理?姜建军:以2016年10月高考试题为例,展开更多
OBJECTIVE To study the effect of the Twist gene on the migration of human hepatocellular carcinoma cells and the possible mechanisms involved. METHODS RT-PCR was used to detect expression of the Twist gene in primary ...OBJECTIVE To study the effect of the Twist gene on the migration of human hepatocellular carcinoma cells and the possible mechanisms involved. METHODS RT-PCR was used to detect expression of the Twist gene in primary （Hep11） and recurrent （Hep12） cell lines from the same HCC patient. Hep11 cells were stably transfected with Twist-cDNA, and Hep12 cells were transiently transfected with Twist RNAi plasmid. Cell migration assays were performed on Twist up-regulated Hep11 cells and Twist RNAi Hep12 cells. RT- PCR and Western blot were used to test the expression of EMT markers. RESULTS Twist was expressed higher level and had increased migration capability in recurrent Hep12 cells than those in primary Hep11 cells. Cell models （Twist-Hep11） in which Twist protein was steadily and highly expressed were obtained. Compared with pcDNA3-Hep11 cells, migration of Twist-Hep11 cells was clearly increased. However, migration of Twist RNAi （Si-Twist-Hep12） Hep12 cells were reduced. Overexpression of Twist in Hep11 cells promoted expression of N-cad and vimentin. CONCLUSION These results indicate that Twist promotes the migration of hepatocellular carcinoma cells in vitro and may play an important role in the upregulation of mesenchymal markers.展开更多
文摘高考读后续写评分的关注点张春青:对于写作题型的关注点,除了如何教,就是如何评价了,评价往往决定教学的方向,我们戏称为The tail wags the dog。姜建军老师和费胜昌老师有非常丰富的评分经验,下面请两位谈谈评分的主要关注点。姜建军:高考试卷中读后续写题总分为25分,按5个档次给分。各档次的给分范围和要求见表1。段湘萍:如何理解这其中的关键词汇呢,如融洽度高、衔接合理?姜建军:以2016年10月高考试题为例,
基金supported by grants from the National Natural Science Foundation of China (No.30671060)Program for New Century Excellent Talent in Universities,China (No.NCET-07-0031)
文摘OBJECTIVE To study the effect of the Twist gene on the migration of human hepatocellular carcinoma cells and the possible mechanisms involved. METHODS RT-PCR was used to detect expression of the Twist gene in primary （Hep11） and recurrent （Hep12） cell lines from the same HCC patient. Hep11 cells were stably transfected with Twist-cDNA, and Hep12 cells were transiently transfected with Twist RNAi plasmid. Cell migration assays were performed on Twist up-regulated Hep11 cells and Twist RNAi Hep12 cells. RT- PCR and Western blot were used to test the expression of EMT markers. RESULTS Twist was expressed higher level and had increased migration capability in recurrent Hep12 cells than those in primary Hep11 cells. Cell models （Twist-Hep11） in which Twist protein was steadily and highly expressed were obtained. Compared with pcDNA3-Hep11 cells, migration of Twist-Hep11 cells was clearly increased. However, migration of Twist RNAi （Si-Twist-Hep12） Hep12 cells were reduced. Overexpression of Twist in Hep11 cells promoted expression of N-cad and vimentin. CONCLUSION These results indicate that Twist promotes the migration of hepatocellular carcinoma cells in vitro and may play an important role in the upregulation of mesenchymal markers.
