桉树焦枯病是世界性的桉树病害,为害桉树的人工林和幼苗,造成严重的经济损失。以Web of Science平台及CNKI为数据来源,采用文献计量的方法,对1993—2016年间桉树焦枯病研究文献的年代分布、主要研究国家、研究机构、作者、主要载文期刊...桉树焦枯病是世界性的桉树病害,为害桉树的人工林和幼苗,造成严重的经济损失。以Web of Science平台及CNKI为数据来源,采用文献计量的方法,对1993—2016年间桉树焦枯病研究文献的年代分布、主要研究国家、研究机构、作者、主要载文期刊、基金资助、学科类别和关键词共现进行分析。结果表明,国内外桉树焦枯病的文献数量总体呈上升趋势,2001年开始进入快速发展阶段。Web of Science统计表明,24 a间共发表论文416篇,澳大利亚的发文量(163篇)、总被引(2 917次)及H指数(28)居世界首位,中国共发表论文27篇,排名世界第5,但发文质量及影响力与其他国家相比还存在较大的差距;发文量最多的研究机构是南非比勒陀利亚大学(79篇),南非比勒陀利亚大学的WINGFIELD M J发文量最高(73篇);该领域的研究涉及植物科学、林学、环境科学与生态学等多个学科,《Plant Disease》是刊文量最多的学术期刊。CNKI上发文101篇,福建农林大学是国内最大的发文机构,福建省在科研投入、科研机构、科研成果具有较大的成效;与国际上的核心作者相比,国内核心作者的论文数量和质量差距较大,可加强相关领域的国际合作。近年来,国内外在研究内容上逐渐向功能基因组等分子生物学领域发展。展开更多
AIM:To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS:Human peripheral-blood-derived mast cells were stimulated with Lacto...AIM:To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS:Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L.rhamnosus) GG (LGG),L.rhamnosus Lc705 (Lc705),Propionibacterium freudenreichii ssp.shermanii JS (PJS) and Bifidobacterium animalis ssp.lactis Bb12 (Bb12) and their combination for 3 or 24 h,and were subjected to global microarray analysis using an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS:LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G,respectively) and histamine H4 receptor.LGG,Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation,and on several genes that encoded proteins with a pro-inflammatory impact,such as interleukin (IL)-8 and tumour necrosis factor alpha.Also genes that encoded proteins with anti-inflammatory functions,such as IL-10,were upregulated.CONCLUSION:Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes,and by inducing a pro-inflammatory response.展开更多
文摘桉树焦枯病是世界性的桉树病害,为害桉树的人工林和幼苗,造成严重的经济损失。以Web of Science平台及CNKI为数据来源,采用文献计量的方法,对1993—2016年间桉树焦枯病研究文献的年代分布、主要研究国家、研究机构、作者、主要载文期刊、基金资助、学科类别和关键词共现进行分析。结果表明,国内外桉树焦枯病的文献数量总体呈上升趋势,2001年开始进入快速发展阶段。Web of Science统计表明,24 a间共发表论文416篇,澳大利亚的发文量(163篇)、总被引(2 917次)及H指数(28)居世界首位,中国共发表论文27篇,排名世界第5,但发文质量及影响力与其他国家相比还存在较大的差距;发文量最多的研究机构是南非比勒陀利亚大学(79篇),南非比勒陀利亚大学的WINGFIELD M J发文量最高(73篇);该领域的研究涉及植物科学、林学、环境科学与生态学等多个学科,《Plant Disease》是刊文量最多的学术期刊。CNKI上发文101篇,福建农林大学是国内最大的发文机构,福建省在科研投入、科研机构、科研成果具有较大的成效;与国际上的核心作者相比,国内核心作者的论文数量和质量差距较大,可加强相关领域的国际合作。近年来,国内外在研究内容上逐渐向功能基因组等分子生物学领域发展。
基金Supported by Valio Research Centre,the Foundation for Nutrition Research,Academy of Finland Research Council for Biosciences and Environment,Grant No.129954Finnish Funding Agency for Technology and Innovation (TEKES) grant No.2243/31/05
文摘AIM:To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS:Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L.rhamnosus) GG (LGG),L.rhamnosus Lc705 (Lc705),Propionibacterium freudenreichii ssp.shermanii JS (PJS) and Bifidobacterium animalis ssp.lactis Bb12 (Bb12) and their combination for 3 or 24 h,and were subjected to global microarray analysis using an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS:LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G,respectively) and histamine H4 receptor.LGG,Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation,and on several genes that encoded proteins with a pro-inflammatory impact,such as interleukin (IL)-8 and tumour necrosis factor alpha.Also genes that encoded proteins with anti-inflammatory functions,such as IL-10,were upregulated.CONCLUSION:Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes,and by inducing a pro-inflammatory response.
