At present in the process of water injection station operation, starting and stopping the pump caused system pressure fluctuations, and the fluctuations caused many problems about downstream injection wells. In order ...At present in the process of water injection station operation, starting and stopping the pump caused system pressure fluctuations, and the fluctuations caused many problems about downstream injection wells. In order to eliminate the fluctuations and reduce problems, taking start pump, connect pump test under pressure in the water injection station installed the rotor frequency control system Changqing oil field developed. During the experiment, by progressively increasing the pressure pump to verify start pump with pressure feasibility test, the result shows when the pressure in the 0-25MPa pressure start and connect pump can be realized, and forecast the maximum pressure of the current start of the station with pressure pump. Start pump with pressure achieve the elimination of pressure fluctuations, provide the prerequisites about realizeing stable water injection pressure, and ultimately realize oil field water injection station to be automatic water injection laid a foundation.展开更多
The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this...The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei （LvPT） was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigenTM design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at -27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA （4 pg per shrimp） was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection （hpi）. Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.展开更多
文摘At present in the process of water injection station operation, starting and stopping the pump caused system pressure fluctuations, and the fluctuations caused many problems about downstream injection wells. In order to eliminate the fluctuations and reduce problems, taking start pump, connect pump test under pressure in the water injection station installed the rotor frequency control system Changqing oil field developed. During the experiment, by progressively increasing the pressure pump to verify start pump with pressure feasibility test, the result shows when the pressure in the 0-25MPa pressure start and connect pump can be realized, and forecast the maximum pressure of the current start of the station with pressure pump. Start pump with pressure achieve the elimination of pressure fluctuations, provide the prerequisites about realizeing stable water injection pressure, and ultimately realize oil field water injection station to be automatic water injection laid a foundation.
基金Supported by the National Basic Research Program of China(973 Program)(No.2012CB114403)the China Agriculture Research System-47(CARS-47)
文摘The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei （LvPT） was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigenTM design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at -27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA （4 pg per shrimp） was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection （hpi）. Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.
