Digital radiographic(DR)testing equipment has been widely promoted and applied in the inspection of circumferential welds in oil and gas pipelines.In order to establish a comprehensive quality control system for digit...Digital radiographic(DR)testing equipment has been widely promoted and applied in the inspection of circumferential welds in oil and gas pipelines.In order to establish a comprehensive quality control system for digital radiographic testing and fully evaluate the integrated system inspection ability of equipment,personnel,and processes,a scientific and standardized evaluation method to the system is very necessary.Here investigates the precedents of relevant non-destructive testing evaluation methods at home and abroad,considers the testing characteristics of DR equipment,develops a complete set of DR testing system evaluation procedures.It deeply studies the adaptability methods of program processes from defect production to slicing processing and data statistical calculation for digital radiographic testing evaluation.To check the repeatability and reliability of the detectable system,five process welds with 200 real metallographic defects were fabricated in the laboratory.From the detected results,the DR system has good repeatability in image quality,and the detectable defect size reaches 0.85 mm under achieving 90%detection probability at a confidence level of 95%,the error of detected defect length is±2 mm,and the error of detected defect localization is±5 mm.The qualitative and quantitative detection of defects are accurate and reliable.The test further confirmed the reliable detection ability of the DR detection system,and fully validated the scientific and practical evaluation method designed.The research on the evaluation test method can serve as an important link in the quality control system for the on-site application of digital ray equipment in long-distance pipelines.The designed program,test,and evaluation content can serve as an important basis for the formulation of relevant specifications or standards.展开更多
To align customer demand with suppliers for reducing work in process and supply costs and increasing re sp onsiveness to customer requirements, a real time collaborative supply chain mana gement system is essential...To align customer demand with suppliers for reducing work in process and supply costs and increasing re sp onsiveness to customer requirements, a real time collaborative supply chain mana gement system is essential. A solution to the realization of supply chain manage ment capable of timely responding to customer requirements is proposed. Workflow automation is used to manage process interaction across enterprises; Agents can be automatically invoked by supply chain workflow process and are used to pro mote the flexibility and reconfigurability by providing the mechanism in support of distributed compution in an enterprises to meet the requirements of performa nce and business dynamics. An approach that supports agent based workflow proce ss in a supply chain is proposed. And the coordination mechanism between agents is also discussed.展开更多
The shortage of organ donors is a problem worldwide, with approximately 15% of adult patients with lifethreatening liver diseases dying while on the waiting list. The use of cell transplantation for liver disease is a...The shortage of organ donors is a problem worldwide, with approximately 15% of adult patients with lifethreatening liver diseases dying while on the waiting list. The use of cell transplantation for liver disease is an attempt to correct metabolic defects, or to support liver function as a bridge to liver transplantation and, as such, has raised a number of expectations. Most of the available studies briefly reported here focus on adult hepatocyte transplantation (HT), and the results are neither reproducible nor comparable, because the means of infusion, amount of injected cells and clinical variability differ among the studies. To better understand the specif ic role of HT in the management of end-stage liver disease, it is important that controlled studies, designed on the principles of evidence-based medicine, be done in order to guarantee the reproducibility of results.展开更多
Nowadays, oil spills have led to a serious environmental crisis of the world. To deal with this problem, inspired from super-hydrophobic lotus leaf, this study fabricated super-hydrophobic and super-lipophilic functio...Nowadays, oil spills have led to a serious environmental crisis of the world. To deal with this problem, inspired from super-hydrophobic lotus leaf, this study fabricated super-hydrophobic and super-lipophilic functionalized graphene oxide/polyurethane （FGP） sponge by a simple and inexpensive dip coating method. The resulting FGP sponge was characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy and water contact angle. The results expressed that FGP sponge exhibited a similar surface structure to that of a lotus leaf, and possessed the super-hydrophobic characteristic with the water contact angle （WAC） of 152°± 1 °. The absorption capacity and reusability were also investigated. It can be seen that, the FGP sponge can remove a wide range of oils and organic solvents from water with good absorption capacities （up to 35 times of its own mass）. Significantly, after 10 cycles the absorption capacity of the oils and organic solvents was higher than 90°; for the reused FGP sponge, demonstrating the good reusability of the FGP sponge. Therefore, this study probably provided a simole way to remove the pollutions ofoil spills and toxic organism from water.展开更多
AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas. METHODS: mRNA was isolated from the hybridoma cell line producing MC3 and the D...AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas. METHODS: mRNA was isolated from the hybridoma cell line producing MC3 and the DNAs encoding variable domains of heavy and light chains (VH and VL) of the antibody were amplified separately by RT-PCR and assembled into ScFv DNA with a linker DNA. The ScFv DNA was ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into E.coli TG1.The transformed cells were infected with M13KO7 helper phage to yield recombinant phages. After two rounds of panning with gastric carcinoma cell line AGS highly expressing MC3-binding antigen, the phage clones displaying ScFv fragments of the antibody were selected by ELISA. 4 phage clones showing strong signal in ELISA were used to infect E.coli HB2151 to express soluble ScFvs. The soluble ScFvs were identified by Dot blot and Western blot, and their antigen-binding activity was assayed by ELISA. The VH and VL DNAs of the ScFv DNA derived from phage clone 19 were sequenced. RESULTS: The VH,VL and ScFv DNAs were about 340 bp, 320 bp and 750 bp respectively. After two rounds of panning to the recombinant phages, 18 antigen-positive phage clones were selected from 30 preselected phage clones by ELISA. All the soluble ScFvs derived from the 4 out of the 18 antigen-positive phage clones were about M(r)32000 and concentrated in periplasmatic space under the given culture condition. The soluble ScFvs could bind the antigen, and they shared the same binding site with MC3. The sequences of the VH and VL DNAs of the MC3 ScFv showed that the variable antibody genes belonged to the IgG1 subgroup,kappa-type. CONCLUSION: The soluble ScFv of MC3 is successfully produced, which not only provides a possible novel targeting vehicle for in vivo and in vitro study on associated cancers, but also offers the antibody a stable genetic source.展开更多
in order to evaluate the capacity of reinforced concrete （RC） structures subjected to blast Ioadings, the damaged plasticity model for concrete was used in the analysis of the dynamic responses of blast-loaded RC st...in order to evaluate the capacity of reinforced concrete （RC） structures subjected to blast Ioadings, the damaged plasticity model for concrete was used in the analysis of the dynamic responses of blast-loaded RC structures, and all three failure modes were numerically simulated by the finite element software ABAQUS. Simulation results agree with the experimental observations. It is demonstrated that the damaged plasticity model for concrete in the finite element software ABAQUS can predict dynamic responses and typical flexure, flexure-shear and direct shear failure modes of the blast-loaded RC structures.展开更多
Background To report quality control methods and baseline reproducibility data of the ultrasound measurements of carotid artery intima-media thickness in the project of Establishment of an Integrated System for Corona...Background To report quality control methods and baseline reproducibility data of the ultrasound measurements of carotid artery intima-media thickness in the project of Establishment of an Integrated System for Coronary Heart Disease Prevention and Treatment. Methods Standard ultrasound scanning and measuring protocols were established by the study group. All sonographers and readers were trained by the carotid ultrasound core lab and all digital ultrasound images were centrally read. Ten subjects were scanned twice （with 1 week interval） by 2 sonographers independently and images were read by a single reader to evaluate the sonographer variability. Twenty subjects＇ images were read twice （with 1 week interval） by a single reader to assess the reader variability and the reproducibility of IMT measured at different carotid segments. Results The intraclass correlation （ICC） of intra- and inter-sonographer and intra- reader for mean IMT measurements was 0.99, 0.98 and 0.97 respectively; while for max IMT, it was 0.97, 0.99 and 0.95 respectively. Among different carotid segments and sites, ICC for mean 1MT measurements of common carotid （CCA）, carotid artery bulb （Bulb）, internal carotid artery （ICA）, overall near wall and overall far wall was 0.97, 0.99, 0.89, 0.93 and 0.98 respectively. Conclusion The reproducibility of IMT measurements according to our protocol is acceptable, although better reproducibility is found when measuring the mean IMT than max IMT, CCA and Bulb IMT than ICA IMT, and far wall IMT than near wall IMT.展开更多
The alkylation of p-cresol with tert-butanol（TBA）to 2-tert-butyl-p-cresol（TBC）catalyzed by a novel multiple-SO3H functioned ionic liquid（IL1）was investigated.Meanwhile,the catalytic activity of this novel ionic ...The alkylation of p-cresol with tert-butanol（TBA）to 2-tert-butyl-p-cresol（TBC）catalyzed by a novel multiple-SO3H functioned ionic liquid（IL1）was investigated.Meanwhile,the catalytic activity of this novel ionic liquid was compared with other four traditional ionic liquids.The results showed that IL1 has superior catalytic activity to other four traditional ionic liquids with the conversion of 85.3%and selectivity of 95.2%.Also,the reaction conditions were investigated to obtain the optimum conditions.Operational simplicity,small amount of usage,high activity,reusability and selectivity are the key features of this methodology.展开更多
文摘Digital radiographic(DR)testing equipment has been widely promoted and applied in the inspection of circumferential welds in oil and gas pipelines.In order to establish a comprehensive quality control system for digital radiographic testing and fully evaluate the integrated system inspection ability of equipment,personnel,and processes,a scientific and standardized evaluation method to the system is very necessary.Here investigates the precedents of relevant non-destructive testing evaluation methods at home and abroad,considers the testing characteristics of DR equipment,develops a complete set of DR testing system evaluation procedures.It deeply studies the adaptability methods of program processes from defect production to slicing processing and data statistical calculation for digital radiographic testing evaluation.To check the repeatability and reliability of the detectable system,five process welds with 200 real metallographic defects were fabricated in the laboratory.From the detected results,the DR system has good repeatability in image quality,and the detectable defect size reaches 0.85 mm under achieving 90%detection probability at a confidence level of 95%,the error of detected defect length is±2 mm,and the error of detected defect localization is±5 mm.The qualitative and quantitative detection of defects are accurate and reliable.The test further confirmed the reliable detection ability of the DR detection system,and fully validated the scientific and practical evaluation method designed.The research on the evaluation test method can serve as an important link in the quality control system for the on-site application of digital ray equipment in long-distance pipelines.The designed program,test,and evaluation content can serve as an important basis for the formulation of relevant specifications or standards.
文摘To align customer demand with suppliers for reducing work in process and supply costs and increasing re sp onsiveness to customer requirements, a real time collaborative supply chain mana gement system is essential. A solution to the realization of supply chain manage ment capable of timely responding to customer requirements is proposed. Workflow automation is used to manage process interaction across enterprises; Agents can be automatically invoked by supply chain workflow process and are used to pro mote the flexibility and reconfigurability by providing the mechanism in support of distributed compution in an enterprises to meet the requirements of performa nce and business dynamics. An approach that supports agent based workflow proce ss in a supply chain is proposed. And the coordination mechanism between agents is also discussed.
文摘The shortage of organ donors is a problem worldwide, with approximately 15% of adult patients with lifethreatening liver diseases dying while on the waiting list. The use of cell transplantation for liver disease is an attempt to correct metabolic defects, or to support liver function as a bridge to liver transplantation and, as such, has raised a number of expectations. Most of the available studies briefly reported here focus on adult hepatocyte transplantation (HT), and the results are neither reproducible nor comparable, because the means of infusion, amount of injected cells and clinical variability differ among the studies. To better understand the specif ic role of HT in the management of end-stage liver disease, it is important that controlled studies, designed on the principles of evidence-based medicine, be done in order to guarantee the reproducibility of results.
基金Supported by the National Natural Science Foundation of China(21776319)
文摘Nowadays, oil spills have led to a serious environmental crisis of the world. To deal with this problem, inspired from super-hydrophobic lotus leaf, this study fabricated super-hydrophobic and super-lipophilic functionalized graphene oxide/polyurethane （FGP） sponge by a simple and inexpensive dip coating method. The resulting FGP sponge was characterized by infrared spectroscopy, X-ray diffraction, scanning electron microscopy and water contact angle. The results expressed that FGP sponge exhibited a similar surface structure to that of a lotus leaf, and possessed the super-hydrophobic characteristic with the water contact angle （WAC） of 152°± 1 °. The absorption capacity and reusability were also investigated. It can be seen that, the FGP sponge can remove a wide range of oils and organic solvents from water with good absorption capacities （up to 35 times of its own mass）. Significantly, after 10 cycles the absorption capacity of the oils and organic solvents was higher than 90°; for the reused FGP sponge, demonstrating the good reusability of the FGP sponge. Therefore, this study probably provided a simole way to remove the pollutions ofoil spills and toxic organism from water.
文摘AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas. METHODS: mRNA was isolated from the hybridoma cell line producing MC3 and the DNAs encoding variable domains of heavy and light chains (VH and VL) of the antibody were amplified separately by RT-PCR and assembled into ScFv DNA with a linker DNA. The ScFv DNA was ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into E.coli TG1.The transformed cells were infected with M13KO7 helper phage to yield recombinant phages. After two rounds of panning with gastric carcinoma cell line AGS highly expressing MC3-binding antigen, the phage clones displaying ScFv fragments of the antibody were selected by ELISA. 4 phage clones showing strong signal in ELISA were used to infect E.coli HB2151 to express soluble ScFvs. The soluble ScFvs were identified by Dot blot and Western blot, and their antigen-binding activity was assayed by ELISA. The VH and VL DNAs of the ScFv DNA derived from phage clone 19 were sequenced. RESULTS: The VH,VL and ScFv DNAs were about 340 bp, 320 bp and 750 bp respectively. After two rounds of panning to the recombinant phages, 18 antigen-positive phage clones were selected from 30 preselected phage clones by ELISA. All the soluble ScFvs derived from the 4 out of the 18 antigen-positive phage clones were about M(r)32000 and concentrated in periplasmatic space under the given culture condition. The soluble ScFvs could bind the antigen, and they shared the same binding site with MC3. The sequences of the VH and VL DNAs of the MC3 ScFv showed that the variable antibody genes belonged to the IgG1 subgroup,kappa-type. CONCLUSION: The soluble ScFv of MC3 is successfully produced, which not only provides a possible novel targeting vehicle for in vivo and in vitro study on associated cancers, but also offers the antibody a stable genetic source.
基金Supported by National Natural Science Foundation of China (No.50638030 and 50525825)National Science and Technology Support Program (No.2006BAJ13B02).
文摘in order to evaluate the capacity of reinforced concrete （RC） structures subjected to blast Ioadings, the damaged plasticity model for concrete was used in the analysis of the dynamic responses of blast-loaded RC structures, and all three failure modes were numerically simulated by the finite element software ABAQUS. Simulation results agree with the experimental observations. It is demonstrated that the damaged plasticity model for concrete in the finite element software ABAQUS can predict dynamic responses and typical flexure, flexure-shear and direct shear failure modes of the blast-loaded RC structures.
文摘Background To report quality control methods and baseline reproducibility data of the ultrasound measurements of carotid artery intima-media thickness in the project of Establishment of an Integrated System for Coronary Heart Disease Prevention and Treatment. Methods Standard ultrasound scanning and measuring protocols were established by the study group. All sonographers and readers were trained by the carotid ultrasound core lab and all digital ultrasound images were centrally read. Ten subjects were scanned twice （with 1 week interval） by 2 sonographers independently and images were read by a single reader to evaluate the sonographer variability. Twenty subjects＇ images were read twice （with 1 week interval） by a single reader to assess the reader variability and the reproducibility of IMT measured at different carotid segments. Results The intraclass correlation （ICC） of intra- and inter-sonographer and intra- reader for mean IMT measurements was 0.99, 0.98 and 0.97 respectively; while for max IMT, it was 0.97, 0.99 and 0.95 respectively. Among different carotid segments and sites, ICC for mean 1MT measurements of common carotid （CCA）, carotid artery bulb （Bulb）, internal carotid artery （ICA）, overall near wall and overall far wall was 0.97, 0.99, 0.89, 0.93 and 0.98 respectively. Conclusion The reproducibility of IMT measurements according to our protocol is acceptable, although better reproducibility is found when measuring the mean IMT than max IMT, CCA and Bulb IMT than ICA IMT, and far wall IMT than near wall IMT.
基金Supported by the National High Technology Research and Development Program of China(2006BAE03B06)Shanghai Leading Academic Discipline Project(B409)Shanghai International Cooperation of Science and Technology Project(06SR07101)
文摘The alkylation of p-cresol with tert-butanol（TBA）to 2-tert-butyl-p-cresol（TBC）catalyzed by a novel multiple-SO3H functioned ionic liquid（IL1）was investigated.Meanwhile,the catalytic activity of this novel ionic liquid was compared with other four traditional ionic liquids.The results showed that IL1 has superior catalytic activity to other four traditional ionic liquids with the conversion of 85.3%and selectivity of 95.2%.Also,the reaction conditions were investigated to obtain the optimum conditions.Operational simplicity,small amount of usage,high activity,reusability and selectivity are the key features of this methodology.
