This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS （surface-enhanced Raman spectroscopy） diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL ...This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS （surface-enhanced Raman spectroscopy） diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL （breakpoint cluster region-Abelson） gene responsible for development of chronic myelogenous leukemia were used as a model system to demonstrate the discrimination between the wild type and Met244Val mutations. The thiolated ssDNA （single-strand DNA） was immobilized on the SERS-active surface and then hybridized to a labeled target sequence from solution. An intense SERS signal of the reporter molecule MGITC was detected from the complementary target due to formation of double helix. The SERS signal was either not observed, or decreased dramatically for a negative control sample consisting of labeled DNA that was not complementary to the DNA probe. The results indicate that our SERS substrate offers an opportunity for the development of novel diagnostic assays.展开更多
Grey theory is a multidisciplinary and generic theory to cope with systems of poor or deficient information. We proposed in this paper an improved grey method (GM) to overcome the disadvantages of the general GM(1,1)....Grey theory is a multidisciplinary and generic theory to cope with systems of poor or deficient information. We proposed in this paper an improved grey method (GM) to overcome the disadvantages of the general GM(1,1). In the improved GM(1,1), a new background value formula is deduced and Markov-chain sign estimation is imbedded into the residual modification model. We tested the efficiency and accuracy of our model by applying it to the power demand forecasting in Taiwan. Experimental results demonstrate the new method has obviously a higher prediction accuracy than the general model.展开更多
Given a start pose and a goal pose, a large number of singularity-free poses are created randomly in the 6 dimensional task space, a short line segment is used to create a feasible path between two singularity-free po...Given a start pose and a goal pose, a large number of singularity-free poses are created randomly in the 6 dimensional task space, a short line segment is used to create a feasible path between two singularity-free poses. A well connected roadmap can be obtained and stored in the 6 dimension task space for a specific 6 DOF parallel manipulator in this way and a singularity-free path is queried to connect the start pose and the goal pose. So the singularity-free path planning between any two given poses for this parallel manipulator can be performed very efficiently. This singularity-free path planning method can be used with any type of parallel manipulator only if the matrix used can be given to define singularities.展开更多
文摘This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS （surface-enhanced Raman spectroscopy） diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL （breakpoint cluster region-Abelson） gene responsible for development of chronic myelogenous leukemia were used as a model system to demonstrate the discrimination between the wild type and Met244Val mutations. The thiolated ssDNA （single-strand DNA） was immobilized on the SERS-active surface and then hybridized to a labeled target sequence from solution. An intense SERS signal of the reporter molecule MGITC was detected from the complementary target due to formation of double helix. The SERS signal was either not observed, or decreased dramatically for a negative control sample consisting of labeled DNA that was not complementary to the DNA probe. The results indicate that our SERS substrate offers an opportunity for the development of novel diagnostic assays.
文摘Grey theory is a multidisciplinary and generic theory to cope with systems of poor or deficient information. We proposed in this paper an improved grey method (GM) to overcome the disadvantages of the general GM(1,1). In the improved GM(1,1), a new background value formula is deduced and Markov-chain sign estimation is imbedded into the residual modification model. We tested the efficiency and accuracy of our model by applying it to the power demand forecasting in Taiwan. Experimental results demonstrate the new method has obviously a higher prediction accuracy than the general model.
文摘Given a start pose and a goal pose, a large number of singularity-free poses are created randomly in the 6 dimensional task space, a short line segment is used to create a feasible path between two singularity-free poses. A well connected roadmap can be obtained and stored in the 6 dimension task space for a specific 6 DOF parallel manipulator in this way and a singularity-free path is queried to connect the start pose and the goal pose. So the singularity-free path planning between any two given poses for this parallel manipulator can be performed very efficiently. This singularity-free path planning method can be used with any type of parallel manipulator only if the matrix used can be given to define singularities.
