[Objective] The aim was to separate and purify antioxidant endophytic fun- gi from Stevia leaves. [Method] Stevia leaves were used as materials for endophytic fungi screening and purification. Then, liquid culture was...[Objective] The aim was to separate and purify antioxidant endophytic fun- gi from Stevia leaves. [Method] Stevia leaves were used as materials for endophytic fungi screening and purification. Then, liquid culture was conducted to test fermented supernatant abilities of antioxidant, DPPH free radical clearing and chemilumines- cence inhibition. [Result] Forty two endophytic fungi were separated from F01 to F42. F41 was proved to be the strongest in antioxidant aspect; F37 was the strongest in DPPH clearing; F02, F07, F20, F25, F37, and F416 stood out in a comprehensive comparison of the above aspects. Flow injection chemiluminescenct method was used to test the above six fermentation liquors, and the results showed that antioxidant abilities of the six were quite strong for all of chemiluminescence rates were over 70%. Through preliminary morphological identification, bacterial colony was found white or yellowy and only F02 was of dissepiment. [Conclusion] Fungi with high-antioxidation activities were separated, which paved the way for future research.展开更多
Endophytic fungus is a rich source of bioactive metabolites with multiple activities.In the present study,eight endophytic fungal strains(Z-1 to Z-8)from Zanthoxylum armatum DC.were isolated and identified,and the ant...Endophytic fungus is a rich source of bioactive metabolites with multiple activities.In the present study,eight endophytic fungal strains(Z-1 to Z-8)from Zanthoxylum armatum DC.were isolated and identified,and the anti-inflammatory effect of their extracts was evaluated in vitro.The results showed that four endophytic fungal extracts(Fusarium oxysporum Schlecht.(Z-1),Alternaria arborescens E.G Simmons(Z-3),Mucor fragilis Bainier(Z-4)and Aspergillus terreus Thom(Z-6))displayed good activities in inhibition of excessive production of nitric oxide in lipopolysaccharide-activated mouse macrophage RAW264.7 cells.Among them,the extracts of Fusarium oxysporum Schlecht showed the maximum inhibition activity with a half maximal inhibitory concentration of 174.0μg/m L.The underlying mechanism of the anti-inflammatory effect of Fusarium oxysporum Schlecht extract was associated with its inhibition on the release of several inflammatory factors.The m RNA expression of interleukin 1β,interleukin 6,inducible nitric oxide synthase in lipopolysaccharide-activated mouse macrophage RAW264.7 cells were all inhibited by Z-1 extract detected by real-time polymerase chain reaction.In summary,several endophytic fungal from Zanthoxylum armatum have good anti-inflammatory activity and Fusarium oxysporum Schlecht possesses a promising potential to be developed to a novel anti-inflammatory agent.展开更多
基金Supported by National Natural Science Foundation of China(31060193)Starting Foundation of Scientific research in Guilin University of Technology~~
文摘[Objective] The aim was to separate and purify antioxidant endophytic fun- gi from Stevia leaves. [Method] Stevia leaves were used as materials for endophytic fungi screening and purification. Then, liquid culture was conducted to test fermented supernatant abilities of antioxidant, DPPH free radical clearing and chemilumines- cence inhibition. [Result] Forty two endophytic fungi were separated from F01 to F42. F41 was proved to be the strongest in antioxidant aspect; F37 was the strongest in DPPH clearing; F02, F07, F20, F25, F37, and F416 stood out in a comprehensive comparison of the above aspects. Flow injection chemiluminescenct method was used to test the above six fermentation liquors, and the results showed that antioxidant abilities of the six were quite strong for all of chemiluminescence rates were over 70%. Through preliminary morphological identification, bacterial colony was found white or yellowy and only F02 was of dissepiment. [Conclusion] Fungi with high-antioxidation activities were separated, which paved the way for future research.
基金The Key Laboratory of Herbal-Tebitan Drug Screening and Deep Processing of Gansu Province(Grant No.20180807)Undergraduate innovation and entrepreneurship training program of Lanzhou University of Technology(Grant No.DC2020249)。
文摘Endophytic fungus is a rich source of bioactive metabolites with multiple activities.In the present study,eight endophytic fungal strains(Z-1 to Z-8)from Zanthoxylum armatum DC.were isolated and identified,and the anti-inflammatory effect of their extracts was evaluated in vitro.The results showed that four endophytic fungal extracts(Fusarium oxysporum Schlecht.(Z-1),Alternaria arborescens E.G Simmons(Z-3),Mucor fragilis Bainier(Z-4)and Aspergillus terreus Thom(Z-6))displayed good activities in inhibition of excessive production of nitric oxide in lipopolysaccharide-activated mouse macrophage RAW264.7 cells.Among them,the extracts of Fusarium oxysporum Schlecht showed the maximum inhibition activity with a half maximal inhibitory concentration of 174.0μg/m L.The underlying mechanism of the anti-inflammatory effect of Fusarium oxysporum Schlecht extract was associated with its inhibition on the release of several inflammatory factors.The m RNA expression of interleukin 1β,interleukin 6,inducible nitric oxide synthase in lipopolysaccharide-activated mouse macrophage RAW264.7 cells were all inhibited by Z-1 extract detected by real-time polymerase chain reaction.In summary,several endophytic fungal from Zanthoxylum armatum have good anti-inflammatory activity and Fusarium oxysporum Schlecht possesses a promising potential to be developed to a novel anti-inflammatory agent.
