[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yel...[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the changes of chlorophyll content, activity and gene expression of caspase-like proteases in the tobacco leaves were studied. [Results] Compared with treatments of white, red and yellow film, blue and purple films delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage, and relatively lowered the activity and gene expression of caspase-like proteases during growth, development and senescence periods. [Conclusion] Different light qualities exhibited various effects on the growth, development and senescence of tobacco leaves, possibly by affecting the activity and gene expression of caspase-like proteases to some extent.展开更多
The expansion of hydroponics in many countries of the world in the last few decades may be ascribed to the ability of soilless growing systems to avoid various problems arising from the use of the soil. Cucumber (Cuc...The expansion of hydroponics in many countries of the world in the last few decades may be ascribed to the ability of soilless growing systems to avoid various problems arising from the use of the soil. Cucumber (Cucurnis sativus L.) and zucchini (Cucurbita pepo L.) plants were grown in closed-soilless culture under unheated-greenhouse conditions at the experimental farm of University of Tuscia, Central Italy to evaluate the effects of four substrates (rockwool, pumice, perlite, and cocofiber) on growth, yield and plant mineral composition. For both cultures, plants grown in cocofiber, perlite and pumice yielded more than those grown in rockwool. The better temperature regime in cocofiber, perlite and pumice was due to the greater thermal inertia compared to rockwool slabs. Thc concentration of N in zucchini and cucumber leaves was significantly higher in cocofiber, perlite and pumice in comparison to the rockwool treatment. The concentration of K was significantly affected by the substrate only for the zucchini squash with the highest value recorded on the organic substrate (cocofiber), whereas the Ca concentration was significantly influenced by the growing media only for cucumber with the highest value observed on pumice. Finally, the lowest Mg concentration in leaf tissue was observed on plants grown with the rockwool substrate for both zucchini squash and cucumber. We can conclude that cocofiber, perlite and pumice are suitable for zucchini and cucumber production in closed soilless system, whereas the use of rockwool is more suitable for crops grown under heated greenhouse conditions.展开更多
H. suaveolens (L.) Poit has been used in folk medicine revealing a wide pharmacological potential. This study aimed to evaluate the efficacy of the essential oil from 11. suaveolens leaves in inhibiting the growth o...H. suaveolens (L.) Poit has been used in folk medicine revealing a wide pharmacological potential. This study aimed to evaluate the efficacy of the essential oil from 11. suaveolens leaves in inhibiting the growth of A. flavus LM257 and synthesis of aflatoxins Bl and B2. The essential oil at 20, 40 and 80 laL/mL strongly reduced the biomass and spore germination ofA. flavus. A 100% inhibition was found at 40 μL/mL and 80μL/mL, while at 20 μL/mL the growth depressed over 50% for both tested parameters. The essential oil at all assayed concentrations totally inhibited the production of aflatoxin B1 and B2. From these results, the essential oil from 11. suaveolens leaves may be considered as an alternative and effective inhibitor ofA. flavus, in addition to offer some protective effect to the production of aflatoxins.展开更多
基金Supported by National Natural Science Foundation of China(30460016)Science and Technology Plan from Yunnan Branch Office of China National Tobacco Corporation(2011YN03,2010YN03,07A01)~~
文摘[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the changes of chlorophyll content, activity and gene expression of caspase-like proteases in the tobacco leaves were studied. [Results] Compared with treatments of white, red and yellow film, blue and purple films delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage, and relatively lowered the activity and gene expression of caspase-like proteases during growth, development and senescence periods. [Conclusion] Different light qualities exhibited various effects on the growth, development and senescence of tobacco leaves, possibly by affecting the activity and gene expression of caspase-like proteases to some extent.
文摘The expansion of hydroponics in many countries of the world in the last few decades may be ascribed to the ability of soilless growing systems to avoid various problems arising from the use of the soil. Cucumber (Cucurnis sativus L.) and zucchini (Cucurbita pepo L.) plants were grown in closed-soilless culture under unheated-greenhouse conditions at the experimental farm of University of Tuscia, Central Italy to evaluate the effects of four substrates (rockwool, pumice, perlite, and cocofiber) on growth, yield and plant mineral composition. For both cultures, plants grown in cocofiber, perlite and pumice yielded more than those grown in rockwool. The better temperature regime in cocofiber, perlite and pumice was due to the greater thermal inertia compared to rockwool slabs. Thc concentration of N in zucchini and cucumber leaves was significantly higher in cocofiber, perlite and pumice in comparison to the rockwool treatment. The concentration of K was significantly affected by the substrate only for the zucchini squash with the highest value recorded on the organic substrate (cocofiber), whereas the Ca concentration was significantly influenced by the growing media only for cucumber with the highest value observed on pumice. Finally, the lowest Mg concentration in leaf tissue was observed on plants grown with the rockwool substrate for both zucchini squash and cucumber. We can conclude that cocofiber, perlite and pumice are suitable for zucchini and cucumber production in closed soilless system, whereas the use of rockwool is more suitable for crops grown under heated greenhouse conditions.
文摘H. suaveolens (L.) Poit has been used in folk medicine revealing a wide pharmacological potential. This study aimed to evaluate the efficacy of the essential oil from 11. suaveolens leaves in inhibiting the growth of A. flavus LM257 and synthesis of aflatoxins Bl and B2. The essential oil at 20, 40 and 80 laL/mL strongly reduced the biomass and spore germination ofA. flavus. A 100% inhibition was found at 40 μL/mL and 80μL/mL, while at 20 μL/mL the growth depressed over 50% for both tested parameters. The essential oil at all assayed concentrations totally inhibited the production of aflatoxin B1 and B2. From these results, the essential oil from 11. suaveolens leaves may be considered as an alternative and effective inhibitor ofA. flavus, in addition to offer some protective effect to the production of aflatoxins.