元宝枫无性系秋季叶片主要成分含量动态变化

为了探究不同表现型无性系叶片呈色机理,以3个不同叶变色类型的(绿、红、黄)元宝枫(Acer truncatum Bunge)的无性系(G-绿、R-红、Y-黄)植株为试验材料,于2011年10月19日—11月11日叶片转色期间分别测定总叶绿素、类胡萝卜素、可溶性糖和花色素苷含量的变化。结果表明:各无性系的叶片在转色前存在较低含量花色素苷,叶片转红是大量合成花色素苷的结果,元宝枫无性系叶片颜色与花色素苷和各色素比例有关,叶片呈色不同主要由基因型决定。不同无性系的可溶性糖含量存在显著差异,无性系R的可溶性糖含量最高,花色素苷的合成可能受高可溶性糖含量的诱导。

Chloroplast Composition and Structural Differences in a Chlorophyll-reduced Mutant of Oilseed Rape Seedlings

对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。电泳结果表明 ,突变体类囊体膜中LHCⅡ和其三聚体LHCⅡ 的含量减少。SDS_PAGE分析显示 ,LHCⅡ的脱辅基蛋白在突变体类囊体膜中明显减少。免疫印迹进一步表明 ,所有LHCⅡ组分的含量仅为野生油菜的类囊体膜的 1 / 3。突变体Cr352 9的天线系统比野生型 352 9的小。

Investigation on Physiological Status of Regional Vegetation Using Pushbroom Hyperspectral Imager Data

To extract vegetation pigment concentration and physiological status has been studied in two test areas covered with swamp and flourish vegetation using pushbroom hyperspectral imager (PHI) data which flied in September of 2000 at Daxing'anling district of Heilongjiang Province, China. The ratio analysis of reflectance spectra (RARS) indices, which were put forward by Chappelle et al (1992), are chosen in this paper owing to their effect and simpleness against both comparison with various methods and techniques for exploration of pigment concentration and characteristics of PHI data. The correlation coefficients between RARS indices and pigment concentration of vegetation were up to 0.8. The new RARS indices modes are established in the two test areas using both PHI data and spectra of different vegetations measured in the field. The indices' parameter images of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Cars) of the test areas covered with swamp and flourish vegetation are acquired by the new RARS indices modes. Furthermore, the regional concentration of Chl a and Chl b are extracted and quantified using regression equations between RARS indices and pigment concentrations, which were built by Blackburn (1998). The results showed the physiological status and variety clearly, and are in good agreement with the distribution of vegetation in the field.

Light-induced Damage of Photosystem ⅡPrimary Electron Donor P680: a High Performance Liquid Chromatographic Analysis of Pigment Content in D1/D2 /Cytochrome b559 Complex Under Photoinhibitory Conditions

ByHPLCanalyticalmethod ,thechangeofPSⅡRC’spigmentcontentintheprocessofphoto damageunderstrongilluminationfromspinach (SpinaciaoleraceaMill.)wascomparativelystudied .Theex perimentalresultsshowthat :(1)Inauthors’analyticalconditions ,(ofwhich ,[Chl]=150 μg/mL ,andthe illuminationstrengthwasputat 2 .3× 10 6 mJ·m- 2 ·s- 1) ,4 5minofilluminationcouldcausealmostthewhole lossofA6 80inthefourthderivativeabsorptionspectra ,whileA6 70decreasedtoaboutonehalfofitsoriginal intensity ;theabsorptionmaximuminred ,concurrently ,wasshiftedfrom 6 76nmto 6 71nm ,representingthe lossofmorethan 90 %ofthephotochemicalactivitiesofthePSⅡRC .(2 )Duringtheperiodofcontinuousil lumination ,theChlconcentrationdecreasedina 3_periodstyle ,whichmeantthatthefirst [Chl]decreasedto the 2 / 3ofitsoriginalamountfrom 2 0minto 4 0minafterilluminationhadstarted ,thenbecamestabilizedup toabout 6 0minofillumination ,thereafteraseconddecreaseof [Chl]inanotherabout2 0minuntilitreached about 30 %oftheoriginallevelandremainedunchangedfromabout 80minon .Theoriginalpigmentcompo nentsofD1/D2 /Cytb559wasapproximatelyas 6Chla∶2Pheo∶2 β_Carwhichareinsupportofauthors’pre viousproposalabouttheminimumChl/Pheoratioof 4∶2inPSⅡRC’spigmentcontents .(3)Afterabout 4 0 minofillumination ,anewlyappearedelutionpeakwasfoundbetweenthePheoandβ_CarpeaksinHPLCpro file ,attheretentiontimeof7.2min ,alittlelaterthanthat (6 .9min)ofPheomolecules ,thenewlyappeared elutionpeakwassupposedtobeakindofaccumulatedandstableproductofthePSⅡRC’sphotodamagepro cessandverymuchpossiblethePheo_likemolecules .

Preliminary Study on Leaf Color Development and Photosynthetic Characteristics of Golden-leaf Ginkgo

[Objective]The aim of this study is to research leaf color development and photosynthetic characteristics of golden-leaf ginkgo.[Method]With one-year-old grafted seedlings of golden-leaf ginkgo as the materials,the changes of leaf color,chlorophyll content（Chl）,carotenoid（x.c）content and rate of chlorophyll and carotenoid content,as well as photosynthetic characteristics under full sunlight and overshadowing were all investigated in this study.[Result]Sprouts of golden-leaf ginkgo were pale-yellow,and changed from orange to golden in April,to light yellow-green in May,to yellow-green from June to October,to yellow in November.The chlorophyll and carotenoid contents and the rates of chlorophyll and carotenoid contents of yellow-leaf were significantly lower than that of green-leaf,while the rate of Chla/x.c and Chlb/x.c was obviously lower than the corresponding pigment content of green-leaf.During the leaf color development,Chla,Chlb and x.c content as well as the rate of Chla/x.c and Chlb/x.c of yellow-leaf ginkgo all increased.The saturation light intensity of ginkgo leaf under natural light was higher than that under overshadowing.Maximum net photosynthetic rate,light compensation point and dark respiration rate of yellow leaf were all significantly higher than that of green leaf.Chla/x.c,Chlb/x.c and Chla＋b/x.c of yellow-leaf ginkgo under overshadowing were higher than the corresponding pigment rate of leaf under natural light.[Conclusion]The best ornamental duration of golden-leaf ginkgo is April and May.The major reason for showing yellow leaf is that Chla/x.c and Chlb/x.c of yellow-leaf is obviously lower than that of green-leaf.During the leaf color development,the major reason for yellow-leaf turning to yellow-green is that the rate of Chla/x.c and Chlb/x.c increases.Chlb content of yellow-leaf under overshadowing is higher than that under natural light,which is an adaptive response to overshadowing.Chla/x.c,Chlb/x.c and Chla＋b/x.c of yellow-leaf under overshadowing are relatively higher,which is one of the reasons why yellow-leaf under overshadowing becomes yellow-green or green.

Relationship Between Leaf Structure and Aloin Content in Six Species of Aloe L.

The leaf structure, content and the storage location of aloin in the leaves of six species of Aloe L. were studied by means of semi-thin section, high performance liquid chromatography (HPLC) and fluorescent microscope. Results showed that all leaves consisted of epidermis, chlorenchyma, aquiferous tissue and vascular bundles. The leaves had the xeromorphic characteristics, including thickened epidermal cell wall, thickened cuticle, sunken stomata and well-developed aquiferous tissue. With the exception of thus, there were remarkable differences in leaf structure among the six species. The chlorenchyma cells were similar to palisade tissues in Aloe arborescens Mill. and A. mutabilis Pillans, but isodiametric in A. vera L., A. vera L. var. chinensis Berg., A. saponaria Hawer and A. greenii Bali. A. arborescens, A. mutabilis, A. very and A. vera var. chinensis included large parenchymatous cells at the vascular bundles, whereas no such cells were observed at the vascular bundles of A. saponaria and A. greenii. In A. arborescens, A. mutabilis and A. vera, the aquiferous tissue sheaths were present and composed of a layer of small parenchymatous cells without chloroplasts around the aquiferous tissue. While there were no aquiferous tissue sheaths in A. vera var. chinensis, A. saponaria and A. greenii. The HPLC revealed that the content of aloin was high in A. arborescens, low in A. vera, and very low in A. saponaria among the six species. The fluorescent microscopy showed that the yellow-green globule only appeared in the large parenchymatous cells of vascular bundles, vascular bundle sheath and aquiferous tissue sheath, but not in the chlorenchyma and aquiferous tissue. Consequently, the large parenchymatous cells of vascular bundles, vascular bundle sheath and aquiferous tissue sheath were the storage location of aloin. They were positively correlated with the content of aloin.

Study on Adaptability of Datura stramonium and Datura stramonium var. tatual to Temperature

This study was conducted to investigate the temperature adaptability of Datura stramonium and D. stramonium var. tatual. Under 4 temperature levels,plantgrowth and pigment concentrations in leaves and stems were determined, and leaf anatomic structures of D. stramonium var. tatual and D. stramonium were analyzed. The results showed that the palisade tissue thickness/sponge tissue thickness,stomatal density and stomatal index values of D. stramonium var. tatual were significantly greater than D. stramonium; the chlorophyll content/carotenoid content in leaves of D. stramonium var. tatual increased at first and decreased then, while no regularity was found in leaves of D. stramonium; and the contents of anthocyanin,flavonoids, and total phenols in stems of D. stramonium var. tatual were significantly higher than those in stems of D. stramonium. The temperature adaptability of D.stramonium var. tatual is stronger than that of D. stramonium.

Comparative Study on Photoinhibition Between Two Wheat Genotypes

Changes in the efficiency of the primary light energy conversion, fluorescence quenching parameters and contents of photosynthetic pigments were compared between two wheat ( Triticum aestivum L.) genotypes in response to high light stress. The contents of chlorophyll and carotenoid in “Jing_411' were slightly higher than those in “Xiaoyan_54'. Under high light stress, photoinhibition as indicated by a sustained decrease in PSⅡ photochemical efficiency was more pronounced in “Jing_411' than in “Xiaoyan_54'. The content of ascorbate and the activity of the deepoxidase were higher in “Xiaoyan_54' than in “Jing_411'. The genotypic difference in resistance to photoinhibition is related to the capacity to dissipate the excess energy nonradiatively.

Effects of Pigment Indicators on the Color Expression of Leaves of the Colored-leaf Garden Plants

[Objective] In this study, the relationship between the pigments and the color expression of leaves of colored-leaf plants was discussed. [Method] The colors of leaf blades of 6 kinds of plants were analyzed with the Royal Horticultural Soci-ety Colour Chart. The chlorophyl content, carotenoids content and anthocyanin con-tent in leaf blades were determined. In addition, the color types of leaf blades, kinds of pigments, pigment contents and pigment distributions of 6 kinds of plants were compared. [Result] The chlorophyl contents ranked as Populus canadensis Moench （green leaves） 〉 Populus deltoids cv. Zhonghuahongye （purple green leaves） 〉 Populus euramericana cv. Quanhong （red leaves）; Acer palmatum Thunb. （green leaves） 〉 Acer palmatum cv. Atropurpureum （purple red leaves） 〉 Acer pal-matum Thunb. cv. Atropurpureum （red leaves）. The ranking of anthocyanin contents was just opposite. The chlorophyl content was negatively related to the anthocyanin content. The leaf color of plants is determined by various pigments. The more the chlorophyl is, the greener the leaf is. The more the anthocyanin is, the redder the leaf is. In the colored-leaf plants, the chlorophyl content represents about 80% of the content of pigments, the carotenoids content represents about 17%, and the an-thocyanin represents about 3%. There is a difference in the chlorophyl content be-tween colored-leaf plants and green plants. However, the relatively low chlorophyl content wil not hamper the normal life activities of colored-leaf plants. [Conclusion] We hoped to provide reference and basis for the production and landscaping of col-ored-leaf plants.

Effect of microbial inoculants on Albizia saman germination and seedling growth

Microbial Inoculants as Effective Microorganisms （EM） were applied to find out their effects on germination and seedling growth of Albizia saman in the nursery. The seedlings were grown in a mixture of sandy soils and cow dung （3：1） kept in polybags. The EM solution at different concentrations （0.1%, 0.5%, 1%, 2%, 5% and 10%） was incorporated before and after a week of sowing seeds. Germination and physical growth parameters, including shoot and root length, vigor index, collar diameter, leaf number, fresh and dry weight of shoot and root and total biomass increment over the control were measured. The nodulation status influenced by EM was also observed along with the estimation of chemical parameters like chlorophyll a, chlorophyll b and carotenoid. Both germination and the measured physical growth parameters were found significantly （P〈0.05） higher in seedlings treated with different concentrations of EM solution in comparison to the control. Maximum growth was found at 2% followed by 1% EM solution. Nodulation was higher at 0.1% concentration but it normally decreased with the increase of concentrations. Although there were a higher amount of pigments in leaves of the treated seedlings than of the control, the variations recorded with respect to chlorophyll a, b and carotenoid were not significantly higher in most of the treatments. Treated seedlings showed variable results along with the increment of EM applications and most of the parameters showed best results at the medium range of concentrations. The study indicates that the Microbial Inoculant （EM） technology might be useful to improve the growth of seedlings in the nursery. This also indicates that the associated beneficial organisms along with the polybag soils might be of value in improving the degraded soil or poor field soil for better nutrient and water uptake during the initial growth of transplanted seedlings.

Effect of Two Herbicides on the Growth of Early Seedlings of Rye(Secale cereale)

[Objective]The aim was to study the effect of herbicide on the growth of early seedlings of rye（Secale cereale）.[Method]Effect of two kinds of herbicide（Atrazine and APM）on seedling growth of rye was investigated at the physiological,biochemical and cellular level.[Result]The Atrazin significantly decreased the contents of chlorophyll a,b and soluble proteins.Rye seeds were treated with 0.01-1 mg/L Atrazine for 16 h,the contents of chlorophyll a and b decreased from 1.26（a）,0.49（b）mg/g FW（control）to 1.15（a）,0.46（b）mg/g FW（0.1 mg/L）and 0.81（a）,0.33（b）mg/g FW（1.0 mg/L）.The content of soluble protein decreased with the increasing concentration of Atrazin.Atrazin had no significant influence on the cell division and chromosome structure variation.The contents of chlorophyll a,b and soluble proteins had no significantly change under the treatment of APM,but the number of chromosome structure variation such as chromosome bridge,multipolar division cells,lagging chromosome and unequal division cells increased significantly.[Conclusion]The critical concentration of Atrazine was 0.1-1.0 mg/L and 4 mg/L of APM in rye.

In situ hyperspectral data analysis for pigment content estimation of rice leaves

Analyses of the correlation between hyperspectral reflectance and pigment content including chlorophyll a, chlorophyll b and carotenoid of leaves in different sites of rice were reported in this paper. The hyperspectral reflectance of late rice during the whole growing season was measured using a Spectroradiometer with spectral range of 350-1050 nm and resolution of 3 nm. The chlorophyll a, chlorophyll b and carotenoid contents in rice leaves in rice fields to which different levels of nitrogen were applied were measured. The chlorophyll a content of upper leaves was well correlated with the spectral variables. However, the correlation between both chlorophyll b and caroteniod and the spectral variables was far from that of chlorophyll a. The potential of hyperspectral reflectance measurement for estimating chlorophyll a of upper leaves was evaluated using univariate correlation and multivariate regression analysis methods with different types of predictors. This study showed that the most suitable estimated model of chlorophyll a of upper leaves was obtained by using some hyperspectral variables such as SD r, SD b and their integration.

Study on the Correlation Between Chlorophyll Maximum and Remote Sensing Data

Based on the in situ optical measurements in the Bohai Sea of China, which belongs to a typical case-2 water area, we studied the characteristics of DCM (deep chlorophyll maximum) such as its spatial distribution, vertical profile, etc. We found that when the depth of the chlorophyll maximum is comparatively small, even in turbid coastal water regions, there is always a good correlation between the concentrations of chlorophyll maximum and the satellite-received signals in blue-green spectral bands; the correlation is even better than that between the surface chlorophyll concentrations and the satellite-received signals. The strong correlation existing even in turbid coastal water regions indicates that an ocean color model to retrieve the concentration of DCM can be constructed for coastal waters if a comprehensive knowledge of the vertical distribution of chlorophyll concentration in the Bohai Sea of China is available.

ASPECTS OF IRON NUTRITION IN MACROALGAE ULVA PERTUSA (CHLOROPHYTA) UNDER IRON STRESS

Fe, Chlorophyll (Chl) and total nitrogen (TN) content in tissues were measured in Fe-deficient cultures of Ulva. pertusa over a period of 60 days. Photosynthetic carbon fixation rates were studied at the start of and 30 days after Fe-deficiency culture, when the effects of Fe-deficiency on the ultrastructure were also analyzed. The iron content in tissue decreased exponentially during Fe-deficiency (from 726.7 to 31.6 μg/gdw) and simultaneously Chl and TN content declined to 4.35% and 59.9% of their original levels respectively. Maximum carbon fixation rate (50-250 μmol/m 2 s) under Fe-deficiency decreased significantly compared with the control (p<0.01) and was 13.6 to 0.365 μg C /cm 2 h. Photosynthesis in Fe-deficient cells became light-saturated at lower irradiance than that in control. Ultrastructural observations of Fe-deficient cells showed reductions in chloroplast number, some degeneration of lamellar organization, an increase in vacuolar area, a decrease in mitochondrial matrix density, and variation in accumulation body number and morphology. During Fe-deficiency, the algae growth rate continued to decline and after 6 weeks of iron deficiency, no further growth was detectable. These suggested that the lower growth rate of Ulva. pertusa under Fe-deficiency could be due mainly to nitrogen utilization and inhibition of photosynthesis.

Carotenoids and Chlorophyll are not the Chromophores of Blue Light-Induced Prehaustoria in Dodder （Cuscuta campestris） Seedlings

Previous studies demonstrated that cryptochromes are involved in blue light-induced coiling and prehaustoria development in young de-etiolated dodder seedlings. In this study, we suggest that carotenoids and chlorophyll are not the blue light absorbing chromophores involved in the mediation of prehaustoria development to blue light. Norflurazon-bleached dodder segments coiled and formed prehaustoria under blue light. However, norflurazon significantly reduced prehaustoria number （62%） under a mixture of red and far-red light, suggesting that phytochromes could be altered by norflurazon.

Molecular evidence suggests that the enigmatic Sulawesi endemic Geomalia heinrichi belongs in the genus Zoothera (Turdidae, Aves)

The taxonomic status of the Sulawesi endemic Geomalia heinrichi has long been debated, and it has variously been treated as a babbler (Timaliidae) or a turdid (Turdidae). We estimated the phylogeny of 43 taxa in the family Turdidae based on the mitochondrial cytochrome b gene and the nuclear myoglobin intron 2 and ornithine decarboxylase introns 6–7. Geomalia heinrichi was shown to be part of the Zoothera clade with high support. We propose that Geomalia is transferred to Zoothera under the name Zoothera heinrichi.

Phenol Compounds of Blackberry （Rubus caucasicus Focke and Rubus anatolicus L.） Fruit and Leaf

It is investigated of the effect of plant blackberry （Rubus caucasicus Focke and Rubus anatolicus L.） flower, fruit and leaf content of phenol compounds qualitative and quantitative HPLC （high-pressure liquid chromatography） and spectral methods. HPLC analysis revealed that the fetus was a major of both species, cyanidin-3-glucoside, and it reached full maturity period 1,493.54 ± 44.81 and 1,636.58 ±49.10 mg/kg （cyanidin-3-O-glucoside chloride equivalents） calculated on the dry weight. In mature fetus flavonols is （884.8±26.54）-（979.0 ± 29.37） mg/kg （rutin equivalents） calculated on the dry weight, catechins （21.2± 0.64）-（25.01 ± 0.7537） mg/kg （（＋） catechin equivalents） calculated on the dry weight, leucoantocyanins （48.8 ±1.46）-（52.1± 1.56） mg/kg （cyanidin equivalents） calculated on the dry weight. In this regard, there was a slight difference between the species. Most of the leaf flavonols were presented 3.7 ± 0.111 g/kg. Phenol carbonic acids--the leaves of 13.4 ± 0.402 mg/kg calculated on the dry weight, the flowers of 8.8± 0.264 mg/kg, calculated on the dry weight. Full maturity period, the number of phenol carbonic acids cent is （3,707.8 ± 111.23）-（4,287.5 ± 128.63） mg/kg, （caffeic acid equivalents） is calculated on the dry weight of the unit. Also been determined HPLC following the method of compounds is： cyanidin-3-glucoside, quercetin-3-rutinoside, quercetin-3-glucoside, ellagic acid, gallic acid and chlorogenic acid.

Site-Specific Nitrogen Management in Dry Direct-Seeded Rice Using Chlorophyll Meter and Leaf Colour Chart

The need to maintain high rice yields and improve fertilizer nitrogen(N)-use efficiency has fueled the use of tools such as leaf colour chart(LCC) and chlorophyll meter(SPAD meter) in managing fertilizer N based on colour of the leaf. Field experiments were conducted during 2011 to 2013 at Ludhiana, India to assess the need for basal N application and to establish critical threshold values of leaf greenness as measured by LCC and SPAD meter for formulating strategies for in-season management of fertilizer N in dry direct-seeded rice(DDSR). Avoiding application of N at sowing did not adversely affect rice grain yield, indicating that basal N application in DDSR was not necessary and might lead to reduced N-use efficiency. Monitoring N uptake rate during the growing season of DDSR suggested that N uptake rate peaked at the two growth stages: maximum tillering(42 to 56 days after sowing(DAS))and panicle initiation stages(70 to 84 DAS). Using the Cate-Nelson procedure, critical LCC and SPAD meter values for fertilizer N application worked out to be 4 and 37, respectively. Real-time fertilizer N management strategy based on applying 30 kg N ha-1whenever SPAD meter or LCC readings fell below the critical values maintained optimum rice yields along with higher N-use efficiency than that observed by following blanket recommendation for fertilizer N in the region. The fixed-time variable-dose strategy consisted of applying prescriptive doses of 20 kg N ha-1at 14 DAS and 30 kg N ha-1at 28 DAS and corrective doses of 30, 40 or 50 kg N ha-1at 49 and 70 DAS depending upon LCC shade to be ≥ 4, 4–3.5, or < 3.5 and SPAD meter readings to be ≥ 40, 40–35, or< 35, respectively. This strategy also resulted in optimal rice yield along with higher N-use efficiency as compared to the blanket recommendation. This study revealed that in DDSR, fertilizer N could be managed more efficiently using the tools of LCC and SPAD meter than the current blanket recommendation.

Transcriptome analysis of three cotton pests reveals features of gene expressions in the mesophyll feeder Apolygus lucorum

The green mirid bug Apolygus lucorum is an agricultural pest that is known to cause damage to more than 150 plant species. Here, we report the transcriptomes ofA. lucorum at three different developmental stages （the second and fifth instar nymphs and adults）. A total of 98,236 unigenes with an average length of 1,335nt was obtained, of which 50,640 were annotated, including those encoding digestive enzymes and cytochrome P450s. Comparisons with cotton bollworm and cotton aphid transcriptomes revealed distinct features ofA. lucorum as a mesophyll feeder. The gene expression dynamics varied during development from young nymphs to adults. The high-quality transcriptome data and the gene expression dynamics reported here provide valuable data for a more comprehensive understanding of the physiology and development of mirid bugs, and for mining targets for their control.