【目的】应用rbcL叶绿体序列解析金线兰及其近缘种亲缘关系。【方法】以血叶兰属及开唇兰属rbcL叶绿体序列设计引物,扩增福建主栽金线兰及其近缘种的DNA序列,并进行系统进化树及序列分析。【结果】从7种金线兰及其近缘种中获得1221 bp r...【目的】应用rbcL叶绿体序列解析金线兰及其近缘种亲缘关系。【方法】以血叶兰属及开唇兰属rbcL叶绿体序列设计引物,扩增福建主栽金线兰及其近缘种的DNA序列,并进行系统进化树及序列分析。【结果】从7种金线兰及其近缘种中获得1221 bp rbcL序列,编码406个氨基酸。对来自19种23条金线兰及近缘种rbcL序列的进化树及序列分析结果表明,金线兰与峨眉金线兰、长裂片金线兰及浙江开唇兰有较近的遗传距离,其次为台湾银线兰,血叶兰与开唇兰属存在较大的遗传距离。23条序列共存在24个多态性位点,及109个变异位点,定义14个单倍型;其中,金线兰与峨眉金线兰、长裂片金线兰及浙江开唇兰共享单倍型,而丽蕾金线兰、滇南开唇兰、滇越金线兰、白线金线兰、钟山金线兰共享单倍型。【结论】rbcL序列可用于金线兰与近缘种的鉴别。展开更多
Several sequences were applied to resolve phylogenetic relationships within Dendranthema and clarify the origin of garden chrysanthemums including sequences of nrDNA ITS, trnT-trnL and trnL-trnF intergenic spacer regi...Several sequences were applied to resolve phylogenetic relationships within Dendranthema and clarify the origin of garden chrysanthemums including sequences of nrDNA ITS, trnT-trnL and trnL-trnF intergenic spacer regions in cpDNA. The relationships among the species are so close that the three sequences could only provide limited information. From the evidence presented, we suggest that: ① D.rhmobifolium be the chloroplast donor of D.vestitum (HN) with the resembling morphology and the same trnT/L IGS sequence. ② D.vestitum, a putative ancestor, may be not the chloroplast donor of garden chrysanthemums. D.lavand-ulifolium might be the chloroplast donor of the type population of D.indicum (HN) or the direct chloroplast donor of the ancient garden chrysanthemum cultivar. ③ D.zawaskii might be not the ancestor of garden chrysanthemums.展开更多
采用Illumina HiSeq X Ten平台测序和生物信息法对唐古特扁桃叶绿体基因组序列特征进行解析。结果表明:唐古特扁桃叶绿体基因组为四分体式结构,完整叶绿体基因组序列全长158166 bp,G+C含量36.8%,注释131个基因,即86个蛋白编码基因、8个r...采用Illumina HiSeq X Ten平台测序和生物信息法对唐古特扁桃叶绿体基因组序列特征进行解析。结果表明:唐古特扁桃叶绿体基因组为四分体式结构,完整叶绿体基因组序列全长158166 bp,G+C含量36.8%,注释131个基因,即86个蛋白编码基因、8个rRNA基因和37个tRNA基因;其叶绿体基因组共有52711个编码密码子,有33种类型是偏好密码子;唐古特扁桃叶绿体基因组中SSR位点有33个,串联重复有13个;SSR中未检测到三核苷酸重复;SSR分布不平衡,大多位于基因间区IGS和LSC区域;基于叶绿体全基因组序列构建系统发育建树,发现唐古特扁桃与同亚属的榆叶梅、野樱桃以及李属毛樱桃亲缘关系较近。展开更多
文摘【目的】应用rbcL叶绿体序列解析金线兰及其近缘种亲缘关系。【方法】以血叶兰属及开唇兰属rbcL叶绿体序列设计引物,扩增福建主栽金线兰及其近缘种的DNA序列,并进行系统进化树及序列分析。【结果】从7种金线兰及其近缘种中获得1221 bp rbcL序列,编码406个氨基酸。对来自19种23条金线兰及近缘种rbcL序列的进化树及序列分析结果表明,金线兰与峨眉金线兰、长裂片金线兰及浙江开唇兰有较近的遗传距离,其次为台湾银线兰,血叶兰与开唇兰属存在较大的遗传距离。23条序列共存在24个多态性位点,及109个变异位点,定义14个单倍型;其中,金线兰与峨眉金线兰、长裂片金线兰及浙江开唇兰共享单倍型,而丽蕾金线兰、滇南开唇兰、滇越金线兰、白线金线兰、钟山金线兰共享单倍型。【结论】rbcL序列可用于金线兰与近缘种的鉴别。
文摘Several sequences were applied to resolve phylogenetic relationships within Dendranthema and clarify the origin of garden chrysanthemums including sequences of nrDNA ITS, trnT-trnL and trnL-trnF intergenic spacer regions in cpDNA. The relationships among the species are so close that the three sequences could only provide limited information. From the evidence presented, we suggest that: ① D.rhmobifolium be the chloroplast donor of D.vestitum (HN) with the resembling morphology and the same trnT/L IGS sequence. ② D.vestitum, a putative ancestor, may be not the chloroplast donor of garden chrysanthemums. D.lavand-ulifolium might be the chloroplast donor of the type population of D.indicum (HN) or the direct chloroplast donor of the ancient garden chrysanthemum cultivar. ③ D.zawaskii might be not the ancestor of garden chrysanthemums.
文摘采用Illumina HiSeq X Ten平台测序和生物信息法对唐古特扁桃叶绿体基因组序列特征进行解析。结果表明:唐古特扁桃叶绿体基因组为四分体式结构,完整叶绿体基因组序列全长158166 bp,G+C含量36.8%,注释131个基因,即86个蛋白编码基因、8个rRNA基因和37个tRNA基因;其叶绿体基因组共有52711个编码密码子,有33种类型是偏好密码子;唐古特扁桃叶绿体基因组中SSR位点有33个,串联重复有13个;SSR中未检测到三核苷酸重复;SSR分布不平衡,大多位于基因间区IGS和LSC区域;基于叶绿体全基因组序列构建系统发育建树,发现唐古特扁桃与同亚属的榆叶梅、野樱桃以及李属毛樱桃亲缘关系较近。
