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龙柏叶绿色素的制备及稳定性试验 被引量:2
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作者 毛燕 王学利 《林产化工通讯》 CAS 2001年第5期16-19,共4页
以龙柏叶为原料 ,经过一系列物理、化学过程 ,制得天然含铜绿色素———叶绿素铜钠 ,并对叶绿素铜钠的稳定性进行了检测 ,从而得出 ,只有 pH值的影响较为显著 ,使叶绿素铜钠保存率有较大的改变。
关键词 龙柏 绿铜钠 稳定性 制备 试验 天然色 叶绿色素 食用色
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叶绿色素不易提取分离的原因及探究办法
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作者 肖洪亮 《教学仪器与实验(中学版)》 2002年第6期27-28,共2页
关键词 叶绿色素 提取 分离 高中教育 生物实验 学习兴趣
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Chloroplast Composition and Structural Differences in a Chlorophyll-reduced Mutant of Oilseed Rape Seedlings 被引量:24
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作者 赵云 杜林方 +2 位作者 杨胜洪 李世崇 张义正 《Acta Botanica Sinica》 CSCD 2001年第8期877-880,共4页
对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。... 对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。电泳结果表明 ,突变体类囊体膜中LHCⅡ和其三聚体LHCⅡ 的含量减少。SDS_PAGE分析显示 ,LHCⅡ的脱辅基蛋白在突变体类囊体膜中明显减少。免疫印迹进一步表明 ,所有LHCⅡ组分的含量仅为野生油菜的类囊体膜的 1 / 3。突变体Cr352 9的天线系统比野生型 352 9的小。 展开更多
关键词 oilseed rape thylakoid membrane pigment protein chlorophyll_reduced mutant
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Investigation on Physiological Status of Regional Vegetation Using Pushbroom Hyperspectral Imager Data 被引量:1
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作者 甘甫平 王润生 +1 位作者 马蔼乃 杨苏明 《Acta Botanica Sinica》 CSCD 2002年第8期983-989,共7页
To extract vegetation pigment concentration and physiological status has been studied in two test areas covered with swamp and flourish vegetation using pushbroom hyperspectral imager (PHI) data which flied in Septemb... To extract vegetation pigment concentration and physiological status has been studied in two test areas covered with swamp and flourish vegetation using pushbroom hyperspectral imager (PHI) data which flied in September of 2000 at Daxing'anling district of Heilongjiang Province, China. The ratio analysis of reflectance spectra (RARS) indices, which were put forward by Chappelle et al (1992), are chosen in this paper owing to their effect and simpleness against both comparison with various methods and techniques for exploration of pigment concentration and characteristics of PHI data. The correlation coefficients between RARS indices and pigment concentration of vegetation were up to 0.8. The new RARS indices modes are established in the two test areas using both PHI data and spectra of different vegetations measured in the field. The indices' parameter images of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Cars) of the test areas covered with swamp and flourish vegetation are acquired by the new RARS indices modes. Furthermore, the regional concentration of Chl a and Chl b are extracted and quantified using regression equations between RARS indices and pigment concentrations, which were built by Blackburn (1998). The results showed the physiological status and variety clearly, and are in good agreement with the distribution of vegetation in the field. 展开更多
关键词 pigment indices pigment concentration CHLOROPHYLL ratio analysis of reflectance spectra (RARS) indices pushbroom hyperspectral imager (PHI) Daxing'anling district
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Light-induced Damage of Photosystem ⅡPrimary Electron Donor P680: a High Performance Liquid Chromatographic Analysis of Pigment Content in D1/D2 /Cytochrome b559 Complex Under Photoinhibitory Conditions 被引量:1
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作者 彭德川 侯建敏 +2 位作者 匡廷云 唐崇钦 汤佩松 《Acta Botanica Sinica》 CSCD 1999年第12期1307-1311,共5页
ByHPLCanalyticalmethod ,thechangeofPSⅡRC’spigmentcontentintheprocessofphoto damageunderstrongilluminationfromspinach (SpinaciaoleraceaMill.)wascomparativelystudied .Theex perimentalresultsshowthat :(1)Inauthors... ByHPLCanalyticalmethod ,thechangeofPSⅡRC’spigmentcontentintheprocessofphoto damageunderstrongilluminationfromspinach (SpinaciaoleraceaMill.)wascomparativelystudied .Theex perimentalresultsshowthat :(1)Inauthors’analyticalconditions ,(ofwhich ,[Chl]=150 μg/mL ,andthe illuminationstrengthwasputat 2 .3× 10 6 mJ·m- 2 ·s- 1) ,4 5minofilluminationcouldcausealmostthewhole lossofA6 80inthefourthderivativeabsorptionspectra ,whileA6 70decreasedtoaboutonehalfofitsoriginal intensity ;theabsorptionmaximuminred ,concurrently ,wasshiftedfrom 6 76nmto 6 71nm ,representingthe lossofmorethan 90 %ofthephotochemicalactivitiesofthePSⅡRC .(2 )Duringtheperiodofcontinuousil lumination ,theChlconcentrationdecreasedina 3_periodstyle ,whichmeantthatthefirst [Chl]decreasedto the 2 / 3ofitsoriginalamountfrom 2 0minto 4 0minafterilluminationhadstarted ,thenbecamestabilizedup toabout 6 0minofillumination ,thereafteraseconddecreaseof [Chl]inanotherabout2 0minuntilitreached about 30 %oftheoriginallevelandremainedunchangedfromabout 80minon .Theoriginalpigmentcompo nentsofD1/D2 /Cytb559wasapproximatelyas 6Chla∶2Pheo∶2 β_Carwhichareinsupportofauthors’pre viousproposalabouttheminimumChl/Pheoratioof 4∶2inPSⅡRC’spigmentcontents .(3)Afterabout 4 0 minofillumination ,anewlyappearedelutionpeakwasfoundbetweenthePheoandβ_CarpeaksinHPLCpro file ,attheretentiontimeof7.2min ,alittlelaterthanthat (6 .9min)ofPheomolecules ,thenewlyappeared elutionpeakwassupposedtobeakindofaccumulatedandstableproductofthePSⅡRC’sphotodamagepro cessandverymuchpossiblethePheo_likemolecules . 展开更多
关键词 PhotosystemⅡ P6 80 Chla D1/D2 /Cytb559 PHOTOINHIBITION HPLC
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Study on the Correlation Between Chlorophyll Maximum and Remote Sensing Data 被引量:1
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作者 XIU Peng LIU Yuguang 《Journal of Ocean University of China》 SCIE CAS 2006年第3期213-218,共6页
Based on the in situ optical measurements in the Bohai Sea of China, which belongs to a typical case-2 water area, we studied the characteristics of DCM (deep chlorophyll maximum) such as its spatial distribution, ver... Based on the in situ optical measurements in the Bohai Sea of China, which belongs to a typical case-2 water area, we studied the characteristics of DCM (deep chlorophyll maximum) such as its spatial distribution, vertical profile, etc. We found that when the depth of the chlorophyll maximum is comparatively small, even in turbid coastal water regions, there is always a good correlation between the concentrations of chlorophyll maximum and the satellite-received signals in blue-green spectral bands; the correlation is even better than that between the surface chlorophyll concentrations and the satellite-received signals. The strong correlation existing even in turbid coastal water regions indicates that an ocean color model to retrieve the concentration of DCM can be constructed for coastal waters if a comprehensive knowledge of the vertical distribution of chlorophyll concentration in the Bohai Sea of China is available. 展开更多
关键词 ocean color remote sensing model deep chlorophyll maximum case-2 water
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ASPECTS OF IRON NUTRITION IN MACROALGAE ULVA PERTUSA (CHLOROPHYTA) UNDER IRON STRESS
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作者 刘静雯 董双林 刘晓云 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2002年第2期162-169,共8页
Fe, Chlorophyll (Chl) and total nitrogen (TN) content in tissues were measured in Fe-deficient cultures of Ulva. pertusa over a period of 60 days. Photosynthetic carbon fixation rates were studied at the start of and ... Fe, Chlorophyll (Chl) and total nitrogen (TN) content in tissues were measured in Fe-deficient cultures of Ulva. pertusa over a period of 60 days. Photosynthetic carbon fixation rates were studied at the start of and 30 days after Fe-deficiency culture, when the effects of Fe-deficiency on the ultrastructure were also analyzed. The iron content in tissue decreased exponentially during Fe-deficiency (from 726.7 to 31.6 μg/gdw) and simultaneously Chl and TN content declined to 4.35% and 59.9% of their original levels respectively. Maximum carbon fixation rate (50-250 μmol/m 2 s) under Fe-deficiency decreased significantly compared with the control (p<0.01) and was 13.6 to 0.365 μg C /cm 2 h. Photosynthesis in Fe-deficient cells became light-saturated at lower irradiance than that in control. Ultrastructural observations of Fe-deficient cells showed reductions in chloroplast number, some degeneration of lamellar organization, an increase in vacuolar area, a decrease in mitochondrial matrix density, and variation in accumulation body number and morphology. During Fe-deficiency, the algae growth rate continued to decline and after 6 weeks of iron deficiency, no further growth was detectable. These suggested that the lower growth rate of Ulva. pertusa under Fe-deficiency could be due mainly to nitrogen utilization and inhibition of photosynthesis. 展开更多
关键词 intracellular nitrogen and iron PIGMENT carbon fixation ULTRASTRUCTURE Ulva.pertusa iron stress MACROALGAE
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Carotenoids and Chlorophyll are not the Chromophores of Blue Light-Induced Prehaustoria in Dodder (Cuscuta campestris) Seedlings
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作者 M. A. Haidar 《Journal of Agricultural Science and Technology(B)》 2011年第3期323-330,共8页
Previous studies demonstrated that cryptochromes are involved in blue light-induced coiling and prehaustoria development in young de-etiolated dodder seedlings. In this study, we suggest that carotenoids and chlorophy... Previous studies demonstrated that cryptochromes are involved in blue light-induced coiling and prehaustoria development in young de-etiolated dodder seedlings. In this study, we suggest that carotenoids and chlorophyll are not the blue light absorbing chromophores involved in the mediation of prehaustoria development to blue light. Norflurazon-bleached dodder segments coiled and formed prehaustoria under blue light. However, norflurazon significantly reduced prehaustoria number (62%) under a mixture of red and far-red light, suggesting that phytochromes could be altered by norflurazon. 展开更多
关键词 Blue light carotenoids DODDER phytochromes.
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Site-Specific Nitrogen Management in Dry Direct-Seeded Rice Using Chlorophyll Meter and Leaf Colour Chart 被引量:5
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作者 Ali Mohamed ALI Harmit Singh THIND +1 位作者 Sandeep SHARMA YADVINDER-SINGH 《Pedosphere》 SCIE CAS CSCD 2015年第1期72-81,共10页
The need to maintain high rice yields and improve fertilizer nitrogen(N)-use efficiency has fueled the use of tools such as leaf colour chart(LCC) and chlorophyll meter(SPAD meter) in managing fertilizer N based on co... The need to maintain high rice yields and improve fertilizer nitrogen(N)-use efficiency has fueled the use of tools such as leaf colour chart(LCC) and chlorophyll meter(SPAD meter) in managing fertilizer N based on colour of the leaf. Field experiments were conducted during 2011 to 2013 at Ludhiana, India to assess the need for basal N application and to establish critical threshold values of leaf greenness as measured by LCC and SPAD meter for formulating strategies for in-season management of fertilizer N in dry direct-seeded rice(DDSR). Avoiding application of N at sowing did not adversely affect rice grain yield, indicating that basal N application in DDSR was not necessary and might lead to reduced N-use efficiency. Monitoring N uptake rate during the growing season of DDSR suggested that N uptake rate peaked at the two growth stages: maximum tillering(42 to 56 days after sowing(DAS))and panicle initiation stages(70 to 84 DAS). Using the Cate-Nelson procedure, critical LCC and SPAD meter values for fertilizer N application worked out to be 4 and 37, respectively. Real-time fertilizer N management strategy based on applying 30 kg N ha-1whenever SPAD meter or LCC readings fell below the critical values maintained optimum rice yields along with higher N-use efficiency than that observed by following blanket recommendation for fertilizer N in the region. The fixed-time variable-dose strategy consisted of applying prescriptive doses of 20 kg N ha-1at 14 DAS and 30 kg N ha-1at 28 DAS and corrective doses of 30, 40 or 50 kg N ha-1at 49 and 70 DAS depending upon LCC shade to be ≥ 4, 4–3.5, or < 3.5 and SPAD meter readings to be ≥ 40, 40–35, or< 35, respectively. This strategy also resulted in optimal rice yield along with higher N-use efficiency as compared to the blanket recommendation. This study revealed that in DDSR, fertilizer N could be managed more efficiently using the tools of LCC and SPAD meter than the current blanket recommendation. 展开更多
关键词 basal N application critical value grain yield leaf greenness N-use efficiency
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