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杂交兰‘紫妍氏’叶艺性状的生理生化与细胞结构分析 被引量:6
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作者 钟淮钦 林榕燕 +2 位作者 吴建设 林兵 叶秀仙 《西北植物学报》 CAS CSCD 北大核心 2021年第7期1165-1174,共10页
该研究以杂交兰‘紫妍氏’(K21)及其3个叶色变异(叶艺)新品系(K21-1、K21-2、K21-3)为材料,分析叶片光合色素含量、叶绿素合成前体物质含量、叶绿素合成相关酶活性及叶绿素荧光参数变化,并进一步观察叶片显微结构和叶绿体超微结构,以探... 该研究以杂交兰‘紫妍氏’(K21)及其3个叶色变异(叶艺)新品系(K21-1、K21-2、K21-3)为材料,分析叶片光合色素含量、叶绿素合成前体物质含量、叶绿素合成相关酶活性及叶绿素荧光参数变化,并进一步观察叶片显微结构和叶绿体超微结构,以探讨其叶色变异的生理基础。结果表明:(1)3个叶艺品系叶片叶艺区域的叶绿素a、叶绿素b、类胡萝卜素含量均显著低于其相应的绿色区域和亲本绿叶;相较于相应的叶片绿色区域,K21-1、K21-2叶片叶艺区域的UrogenⅢ大量积累,K21-3叶片叶艺区域的PBG大量积累;3个叶艺品系叶片叶艺区域中的光系统Ⅱ最大光化学效率(Fv/Fm)和实际光化学效率[Y(Ⅱ)]均显著低于叶片绿色区域,三者的光化学淬灭系数(qP)则显著高于叶片绿色区域。(2)在显微结构和叶绿体超微结构比较中,相较于叶片绿色区域,叶片叶艺区域的细胞中含较少的叶绿体,且叶绿体的发育成熟程度较低,其中K21-3呈现出一种空腔化的结构。研究推测,叶绿素前体物质合成受阻、叶绿体结构发育不良和叶绿素含量下降是杂交兰3个叶艺品系叶艺形成的原因。该研究从生理和细胞层面初步解释了杂交兰叶艺形成的可能原因,为进一步开展相关分子机理研究及合理利用种质资源奠定了理论基础。 展开更多
关键词 杂交兰 叶艺性状 光合色素 荧光参数 显微结构 叶绿体超微结构
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杂交兰叶艺品系主要生理特性的比较 被引量:4
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作者 林榕燕 吴建设 +2 位作者 林兵 叶秀仙 钟淮钦 《江苏农业科学》 北大核心 2021年第13期136-140,共5页
为探明叶色变异对杂交兰叶片主要生理特性的影响,对杂交兰紫妍氏及其3个叶艺品系叶片的可溶性糖含量、丙二醛(MDA)含量、相对电导率、抗氧化酶活性及三磷酸腺苷酶(ATP)活性等进行比较分析。结果表明,相较于对照组(K21绿色叶片、K21-1和K... 为探明叶色变异对杂交兰叶片主要生理特性的影响,对杂交兰紫妍氏及其3个叶艺品系叶片的可溶性糖含量、丙二醛(MDA)含量、相对电导率、抗氧化酶活性及三磷酸腺苷酶(ATP)活性等进行比较分析。结果表明,相较于对照组(K21绿色叶片、K21-1和K21-2绿色区叶片),3个叶艺品系叶艺区叶片中ATP活性均有所下降,而超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和相对电导率、丙二醛含量均有所提升。此外,相关性分析结果显示,相对电导率、SOD活性和CAT活性与叶绿素a、叶绿素b、总叶绿素含量存在显著或极显著负相关关系;而POD活性、MDA含量、ATP活性、可溶性糖含量与叶绿素a、叶绿素b和总叶绿素含量不存在显著相关关系。由此推测叶色变异对相对电导率和抗氧化酶活性影响较大,抗氧化酶活性的提高有利于清除代谢产生的有害物质,防止膜脂过氧化加剧,对维持植物的正常生理代谢具有积极的作用。本研究结果为进一步开展杂交兰叶艺形成机制研究奠定了基础。 展开更多
关键词 杂交兰 丙二醛含量 相对电导率 抗氧化酶 相关性分析 叶艺性状
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Physical-chemical and Agronomical Analysis of a Leaf Color Mutant of Rice
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作者 王丽 赵德明 +2 位作者 李云武 杨丛金 王峰 《Agricultural Science & Technology》 CAS 2010年第9期168-170,共3页
[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before head... [Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant. 展开更多
关键词 MUTANT CHLOROPHYLL Soluble sugar Agronomic traits
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Effects of the Combined Use of Tobacco Special Inorganic Fertilizer and Cake Organic Fertilizer on the Quality of Tobacco K326 被引量:2
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作者 曾维爱 龙世平 +3 位作者 何命军 范海珊 崔新卫 黄艳宁 《Agricultural Science & Technology》 CAS 2013年第8期1142-1146,共5页
[Objective] This study aimed to investigate the effects of the combined use of cake organic fertilizer and tobacco special fertilizer on the growth and development as well as the quality of tobacco leaves.[Method] Dif... [Objective] This study aimed to investigate the effects of the combined use of cake organic fertilizer and tobacco special fertilizer on the growth and development as well as the quality of tobacco leaves.[Method] Different ratios of cake organic fertilizer and tobacco special fertilizer were applied.Then,the agronomic traits and leaf quality determining factors were measured and analyzed at different growing stages.[Result] Compared with the control,the tobacco plant height was increased by 3.01%-5.84%,while the number of valid leaves was increased by 5.56%-20.39% in all the treatments with combined use of fertilizers.The ratio of sugar to nicotine of leaf was closer to 10±1,and the ratio of nitrogen to nicotine of leaf was closer to 1±0.1.In addition,the combined use of 750 kg/hm2 tobacco special base fertilizer and 292 kg/hm2 cake organic fertilizer had a better effect for improving the agronomic traits and flavor qualities than all the other treatments.[Conclusion] This study will provide reference for developing special full-aroma tobacco leaf product and new tobacco special fertilizers in Hunan. 展开更多
关键词 Cake fertilizer Tobacco special fertilizer Flue-cured tobacco leaf Agronomic trait Flavor quality
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