Scheme of Nitrogen Reasonable Fertilization in Mulberry Gardens of Sichuan Hilly Areas

Abstract In the research, five test groups were set, including urea at O, 120, 240, 360 and 480 kg/hm^2, respectively, and analysis was conducted on mulberry leaf yield and some characters of the quality in order to establish utility functions of ap-plied N in mulberry gardens with different production purposes and to provide schemes of rational N fertilization. The results indicated that yield of mulberry leaf and content and yield of active nutritional substances in the leaves would be reduced either by insufficient or excessive N quantity. In addition, 240 kg/hm^2 of applied N significantly improved active nutritional substances in leaf and 360 kg/hm^2 of applied N significantly improved yields of mulberry leaf and active nutritional sub-stances. Therefore, utility functions of applied N quantity with leaf yield, DNJ content and yield were established accordingly. The optimal N quantity in mulberry gardens for sericulture is recommended 360 kg/hm^2 and the optimal N quantities in mulberry gardens for increase of yield and content of DNJ are 299.56 and 294.72 kg/hm^2, respectively.

Effect of Slash Burning on Nutrient Removal and Soil Fertility in Chinese Fir and Evergreen Broadleaved Forests of Mid-Subtropical China

A Chinese fir forest (Cunninghamia lanceolata, CF) and an evergreen broadleaved forest (EB) located inFujian Province, southeastern China, were examined following slash burning to compare nutrient capital andtopsoil properties with pre-burn levels. After fire, nutrient (N, P and K) removal from burning residues wasestimated at 302.5 kg ha-1 in the CF and 644.8 kg ha-1 in the EB. Fire reduced the topsoil capitals of totalN and P by about 20% and 10%, respectively, in both forests, while K capital was increased in the topsoils ofboth forests following fire. Total site nutrient loss through surface erosion was 28.4 kg (N) ha-1, 8.4 kg (P)ha-1 and 328.7 kg (K) ha-1 in the CF. In the EB, the losses of total N, P and K were 58.5, 10.5 and 396.3kg ha-1, respectively. Improvement of soil structure and increase in mineralization of nutrients associatedwith increased microbe number and enzyme activities and elevated soil respiration occurred 5 days after fire.However, organic matter and available nutrient contents and most of other soil parameters declined one yearafter fire on the burned CF and EB topsoils. These results suggest that short-term site productivity canbe stimulated immediately, but reduced subsequently by soil and water losses, especially in South China,where high-intensity precipitation, steep slopes and fragile soil can be expected. Therefore, the silviculturalmeasurements should be developed in plantation management.

Tea-Grown Soils and Tea Quality in Sichuan and Chongqing, China

Made teas and typical tea-grown soils in Sichuan and Chongqing were collected to investigate soil nutrients, related soil properties and tea quality. The tea-grown soils in Sichuan and Chongqing are distributed mainly in mountainous areas. The high annual precipitation (over 1100 mm), precipitous soil slopes, low cohesion among soil particles and high soil porosity suggested that intensive erosion and leaching might occur in these soils. Moreover, they were very acidic and poor in mineral nutrients such as N, P, K, Ca and Mg except S. The average content of total S was 20.40 g kg-1, much higher than that of organic matter in these soils, revealing that S in the tea-grown soils existed mainly in inorganic forms and very little in organic forms. Water-extractable S accounted for only a small amount of total S, which showed that most parts of sulfur in these soils were insoluble in W8ter. K and S varied greatly in made teas. The concentrations of N and P, however, varied little in these teas even though they differentiated significantly in the tea-grown soils. The high concentration of nitrogen in made teas could result in the high free amino acids and low polyphenol of teas. Significantly positive correlation was established between potassium and polyphenol in made teas. Teas with high ratio of phenol to free amino acids were usually good in taste and appearance.

Relationship between the Water Body Chlorophylla and Water Quality Factors of Wetlands Baiguishan Reservoir

[Objective] The aim was to explore the relationship between water body Chlorophyll-a and water quality factors of wetlands Baiguishan reservoir.[Method] Chlorophyll-a and water quality factors of water quality of Wetlands BaiGuishan Reservoir was studied,the analysis of the relationship on water quality of Wetlands Baiguishan Reservoir was made by use of trophic status indices and SPSS17.0 statistical analysis.[Result] Total phosphorus was an important factor of influence Chlorophyll-a in reservoir,water body had slight eutrophication phenomenon in reservoir of July to October in 2010.[Conclusion] Comprehensive management should be strengthened so as to improve the water quality of Baiguishan wetland.

Alginate-Casein Microspheres as Bioactive Vehicles for Nutrients

The aim of this work was to develop an alginate-casein composite microsphere as a bioaetive vehicle for oral administration of nutrients by a simple extrusion dripping method. Riboflavin was selected as a model drug, and the microencapsulation efficiency was raised to 97.94% after optimizing the preparation conditions by response surface methodology. In vitro release studies showed that riboflavin was released completely from alginate-casein microspheres in simulated intestinal fluids. Meanwhile, the morphology, structure and interaction between alginate and casein were characterized by scanning electron microscopy （SEM） and Fourier transform infrared （FTIR） spectra.

A 2011 drought event affecting distribution of nutrients and chlorophyll in the Zhujiang River estuary

During the summer of 2011, a severe drought event occurred throughout the Zhujiang （Pearl） River Basin of South China. This decreased runoffinto the river, resulting in increased salinity and reduced suspended substance. To examine the effects of this extreme drought on the distribution of nutrients and chlorophyll, we compared two surveys from 2006 and 20ll. Results show that dissolved inorganic nitrogen concentration did not change from 2006 to 2011, whereas the proportions of NOR and NH4＋ to DIN in 2011 increased. PO4 3- concentration was lower in 2011 than in 2006, whereas there was no difference in SiO3 2- concentration between these years. Correlation coefficients of salinity with levels of NO3-, NO2-, NH4＋, PO4 3- and SiO3 2- in 2011 were all much higher than those in 2006, suggesting greater conservation of dissolved nutrients during the extreme drought event. Furthermore, calculated amounts of regenerated nitrate and phosphorus and their proportions to observed nutrients in 2011 were much lower than in 2006, indicating that nutrient regeneration decreased during the extreme drought period. Mean concentration of chlorophyll a （Chl-a） was considerably higher in 2011 than in 2006, and a harmful algal bloom of Cochlodinium geminatum was observed in the estuary, owing to water stagnancy and lower turbidity as a consequence of drought. Therefore, the extreme drought event in 2011 changed the composition ratio of nutrients, enhanced nutrient conservative behavior, and reduced nutrient regeneration. This affected some key ecological processes in the estuary.

Collection and analysis of stem exudate from soybean seedling

Vascular tissue serves as the channel for nutrient transport and signal transduc-tion between different organs in plants. To study molecular identity and behavior of mobile substances transmitted between organs via vascular tissue, it is necessary to collect exudate from stem or other organs. Modifed stem-cutting method for exudate collection in soybean was used in this study by selecting the optimum sampling time and position, using reagents preventing RNA degradation, etc. Diurnal dynamics analysis of exudate emission was found to be the highest during 10：00-10：10 am. Totally 15 μL pure exudate was collected from the stem cut between cotyledonary and unifoliolate nodes at V1 stage （unifoliolate just expanded） of young soybean seedling. Improved TRIzol method was used to extract RNA and protein from stem exudate. A phloem specifc gene of Glycine max sieve element occlusion s, SEO, in exudate samples was successfully amplifed by RT-PCR, which comfrmed the success of RNA extraction. SDS-PAGE showed the majority of proteins in exudate were of low molec-ular weight. Method proposed in this study would facilitate collection of quality exudate and enhance further investigation of mobile substances in soybean.

The Advanced Approaches to Nutritional and Breadmaking Quality of Wheat, Barley and Rye Flour

This work is focused on the characterization and rapid analytical determination of cereal flour quality with regard to nutritional and breadmaking quality. Starch, protein and non-starch polysaccharides are the main components of cereals. The content and quality of proteins and content of damaged starch is important because of the technological quality of flours. The high content of high molecular weight proteins is substantial for bread technology especially, while soluble protein fractions and non-starch polysaccharides are important for nutrition. The set of wheat, barley and rye flours and their blends were analyzed and their properties and their qualitative parameters were determined. Principal component analysis （PCA） was used on Fourier transform-infrared （FT-IR） spectra in the 1,200-800 cm1 wavenumber region and significant correlations of various nutritional and breadmaking parameters were observed. Results showed that the FT-IR spectroscopy and PCA can serve for rapid screening and classification of cereal flour quality.