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外源NO对铅胁迫下三叶鬼针草幼苗活性氧代谢的影响 被引量:13
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作者 杨志娟 陈银萍 +3 位作者 苏向楠 郑怡 蘧苗苗 子轩 《广西植物》 CAS CSCD 北大核心 2015年第5期648-655,共8页
铅(Pb)是已知毒性最强的重金属污染源之一,一氧化氮(NO)是一种普遍存在于生物界的信使分子,广泛参与植物对Pb胁迫的应答反应调控,而三叶鬼针草(Bidens pilosa)是修复Pb污染的重要种质资源。为了进一步探明NO在植物Pb胁迫响应中的作用及... 铅(Pb)是已知毒性最强的重金属污染源之一,一氧化氮(NO)是一种普遍存在于生物界的信使分子,广泛参与植物对Pb胁迫的应答反应调控,而三叶鬼针草(Bidens pilosa)是修复Pb污染的重要种质资源。为了进一步探明NO在植物Pb胁迫响应中的作用及机理,增强三叶鬼针草对Pb污染土壤的耐性以及更好地应用于Pb污染土壤的修复。该研究以培养60 d的三叶鬼针草幼苗为材料,用不同浓度NO供体硝普钠(SNP)预处理,测定600μmol/L硝酸铅胁迫处理第3天三叶鬼针草叶、茎和根的膜质过氧化、抗氧化酶系统和渗透调节物质含量等指标,分析外源NO对铅胁迫下三叶鬼针草活性氧代谢的影响。结果表明:300μmol/L SNP能显著降低铅胁迫下三叶鬼针草相对电导率(REC)、丙二醛(MDA)、过氧化氢(H2O2)含量以及超氧阴离子(O-2·)产生速率;能显著促进脯氨酸(Pro)、可溶性蛋白(SP)、类胡萝卜素(Car)的合成;和不同浓度SNP对三叶鬼针草叶、茎和根中抗氧化酶活性的影响较复杂,其中200μmol/L SNP能显著增强叶和茎中抗坏血酸氧化酶(APX)活性、茎中谷胱甘肽还原酶(GR)活性,300μmol/L SNP能显著增强叶中过氧化物酶(POD)活性,1000μmol/L SNP能显著增强茎和根中过氧化氢酶(CAT)活性和叶中超氧化物歧化酶(SOD)活性。综上表明,适宜浓度NO可以通过启动抗氧化系统,增加渗透调节物质含量和调节抗氧化酶活性,从而有效保护三叶鬼针草膜系统稳定性,缓解Pb胁迫伤害。因此,适宜浓度NO可以增强三叶鬼针草对Pb污染土壤的耐性,其在三叶鬼针草修复Pb污染土壤中具有较好的应用价值。 展开更多
关键词 一氧化氮(NO) 叶鬼针草 膜质过氧化 抗氧化酶系统 渗透调节物质
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鬼针草叶治疗非增生型糖尿病视网膜病变的临床研究 被引量:11
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作者 邵毅 周琼 +5 位作者 易昀敏 余瑶 裴重刚 吴晓蓉 梅峰 占敏艳 《眼科新进展》 CAS 北大核心 2013年第6期531-534,共4页
目的观察鬼针草叶治疗非增生型糖尿病视网膜病变的临床疗效。方法前瞻性随机对照研究。32例(64眼)非增生型糖尿病视网膜病变患者随机分为A、B两组,在严格控制血糖及血压基础上,A组口服鬼针草叶(15.0g),B组口服安慰剂维生素C片0.1g,均每... 目的观察鬼针草叶治疗非增生型糖尿病视网膜病变的临床疗效。方法前瞻性随机对照研究。32例(64眼)非增生型糖尿病视网膜病变患者随机分为A、B两组,在严格控制血糖及血压基础上,A组口服鬼针草叶(15.0g),B组口服安慰剂维生素C片0.1g,均每日3次,饭前0.5h口服,两组均常规口服羟苯磺酸钙分散片0.5g,每日3次,连续服用21d。观察患者治疗前后视力、眼底荧光血管造影、眼底照相及中医症候的变化。结果治疗21d后,视力提高38眼,其中A组24眼(75.0%),B组14眼(43.8%),两组比较差异有统计学意义(P<0.05);32眼治疗后最佳矫正视力>0.05,其中A组22眼(68.8%),B组10眼(31.2%),两组比较差异有统计学意义(P<0.05)。A组眼底病变治疗有效率为31.3%,总有效率81.3%;B组有效率21.9%,总有效率65.6%,两组有效率比较差异有统计学意义(P<0.05)。A组中医症候显效率31.3%,总有效率81.3%;B组显效率18.8%,总有效率62.5%,两组总有效率比较差异有统计学意义(P<0.05)。结论鬼针草叶可提高非增生型糖尿病视网膜病变患者视力,有效改善其眼底病变及减轻中医症候。 展开更多
关键词 鬼针草 视力 中医症候 治疗 非增生型糖尿病视网膜病变
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鬼针草叶治疗更年期女性中重度干眼症临床研究 被引量:29
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作者 邵毅 余瑶 +4 位作者 黄国栋谭钢.,裴重刚',刘新华' 黄国栋 谭钢 裴重刚 刘新华 《中国中药杂志》 CAS CSCD 北大核心 2012年第19期2985-2989,共5页
目的:研究鬼针草叶治疗更年期女性中重度干眼的临床效果。方法:该研究为前瞻性随机对照研究。96例(96眼)女性中重度干眼症患者(40~50岁)被随机分为2组:A组鬼针草叶(48例)和B组维生素C(48例)。2组均加用羟糖甘滴眼液滴眼治疗;分别在治... 目的:研究鬼针草叶治疗更年期女性中重度干眼的临床效果。方法:该研究为前瞻性随机对照研究。96例(96眼)女性中重度干眼症患者(40~50岁)被随机分为2组:A组鬼针草叶(48例)和B组维生素C(48例)。2组均加用羟糖甘滴眼液滴眼治疗;分别在治疗前和治疗后3,7,28 d各时间点检测并评定患者干眼主观症状、OSDI、泪膜4项,治疗前后及2组间均进行方差分析和样本均数或中位数差值分析。结果:治疗前,2组患者各症状、检测指标之间差异无统计学意义;治疗28d后,鬼针草叶组中重度干眼的样本均数差,均较治疗前明显改善,OSDI及泪膜4项较治疗前也均有不同程度改善;维生素C组治疗28 d后,中重度干眼的样本均数差值,均较治疗前无明显改善,OSDI及泪膜4项较治疗前也未见明显好转;2组患者OSDI,BUT,SIT,泪河高度及FL相比,差异均有显著性。结论:鬼针草叶可有效减轻中重度更年期干眼女性的症状和体征,具有一定的临床意义。 展开更多
关键词 鬼针草 干眼 治疗 女性
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Investigation of Types and Hazard of Weeds in Coffea arabica Orchads in Nujiang River Basin 被引量:4
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作者 胡发广 李荣福 +6 位作者 毕晓菲 龙亚芹 郭容琦 俞艳春 李贵平 王万东 周迎春 《Agricultural Science & Technology》 CAS 2012年第11期2367-2369,共3页
[Objective] Through the investigation of weeds in Coffea arabica orchads in NuJiang River basin, this study aimed to provide scientific guidance for the weed control and improving the ecological and economic benefits ... [Objective] Through the investigation of weeds in Coffea arabica orchads in NuJiang River basin, this study aimed to provide scientific guidance for the weed control and improving the ecological and economic benefits of the plantation of Cof- fea arabica. [Method] The types of weeds and the characteristics of weed occur- rence in Coffea arabica orchads in Nujiang River basin were investigated from July to August in 2012. [Result] The results showed that there were 69 types of weeds belonging to 21 families in Coffea arabica orchads. The predominant harmful types were shown as follows: Commelina nudiflora L. + Leptochloa chinensis, Cyperus ro- tundus L. + Bidens pilosa L. + Eleusine indica, Imperata cylindrical + Ageratum conyzoides L. + Eupatorium odoratum L., Ageratum conyzoides L. + Digitaria san- guinalis (L.) Scop. The preponderant weeds consisted mainly of 10 species, namely, Cyperus rotundus L., Commelina nudiflora L., Leptochloa chinensis, Digitaria san- guinalis (L.) Scop, Imperata cylindrical, Bidens pilosa L., Ageratum conyzoides L, E- upatorium odoratum L., Eleusine indica and Chenopodium serotinum L. [Conclusion] The result from this study is of great significance for the plantation of Coffea arabica in Nujiang River basin, as well as the control of weeds. 展开更多
关键词 INVESTIGATION Coffea arabica Types and hazard of weeds Nujiang Riverbasin
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纸色谱法实验教学的研究 被引量:1
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作者 徐怀春 刘俊 赵宁东 《广东化工》 CAS 2013年第17期220-222,共3页
目的:研究鬼针草叶代替菠菜叶开设从菠菜叶中提取叶绿素a、叶绿素b、叶绿素c以及β-胡萝卜素、类β-胡萝卜素的纸色谱法实验。方法:纸色谱法实验。结论:用5~10g新鲜的鬼针草叶与8~10gCaO、2gsi02和适当的丙酮混研,简单过滤制备的... 目的:研究鬼针草叶代替菠菜叶开设从菠菜叶中提取叶绿素a、叶绿素b、叶绿素c以及β-胡萝卜素、类β-胡萝卜素的纸色谱法实验。方法:纸色谱法实验。结论:用5~10g新鲜的鬼针草叶与8~10gCaO、2gsi02和适当的丙酮混研,简单过滤制备的试样进行纸色谱实验,效果较好。该实验的方法可以为开展中学化学、生物课外实验,寻找各种替代材料(云南省是植物王国,材料相当丰富)提供思路。 展开更多
关键词 鬼针草 试剂制备 层析液选择 教学研究
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Allelopathic Potential of Orange Jessamine (Murraya paniculata L.) against Weeds
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作者 Udomporn Pangnakorn Thanatchasanha Poonpaiboonpipattana 《Journal of Agricultural Science and Technology(A)》 2013年第10期790-796,共7页
The use of allelopathic plants is an alternative technique for controlling weeds in sustainable crop production. This experiment evaluated the allelopathic potential of Murraya paniculata (L.) against four weeds viz... The use of allelopathic plants is an alternative technique for controlling weeds in sustainable crop production. This experiment evaluated the allelopathic potential of Murraya paniculata (L.) against four weeds viz., Bidens pilosa L., Amarathus spinosus L., Echinochloa crusgalli (L.) Beauv. and Chloris barbata Sw.. In laboratory test, aqueous extracts from M. paniculata leaves at concentrations of 12.5, 25, 50 and 100 g/L were tested on germination and seedling growth of the weeds. The extracts showed inhibitory effect, varying with weed species and extract concentration. Increase in concentrations increased the degree of inhibition. Concentration at 50 g/L and 100 g/L, completely inhibited seed germination of C. barbara and A. spinosus. Pot experiments were done in a glasshouse, with dry leaf powder as mulch on the soil surface at levels of 2, 4 and 8 t/ha. Dry leaf powder reduced the emergence and growth of all tested weeds, varying with dose and species. Chloris barbara is the most sensitive to M paniculata leaf mulch while E. crus-galli is the most resistant. These results suggest that M. paniculata had the potential lbr weed control and might be the source of a natural herbicide. 展开更多
关键词 ALLELOPATHY Murraya paniculata (L.) aqueous extracts soil mulching weed control.
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鬼针草叶水及二氯甲烷提取物对果糖致高血压大鼠的疗效
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作者 崔翰明 《国外医药(植物药分册)》 2002年第3期115-115,共1页
鬼针草 Bidebs pilosa L.是非洲广泛应用的传统草药。民间应用及药理实验均显示其有抗高血压活性。作者评价了该植物叶的水和二氯甲烷提取物对大鼠大量喂饲果糖引起的高血压及代谢异常是否具防治作用。该植物鲜叶晒干粉碎后用蒸馏水浸泡... 鬼针草 Bidebs pilosa L.是非洲广泛应用的传统草药。民间应用及药理实验均显示其有抗高血压活性。作者评价了该植物叶的水和二氯甲烷提取物对大鼠大量喂饲果糖引起的高血压及代谢异常是否具防治作用。该植物鲜叶晒干粉碎后用蒸馏水浸泡24h,过滤、冻干得水提物。叶风干、粉碎后加甲醇-二氯甲烷(1∶1)浸渍,过滤,滤液减压除去溶媒后再用二氯甲烷提取。 展开更多
关键词 鬼针草 药理学 鬼针草 二氯甲烷提取物 高血压 疗效
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Flavonoids from the herb of Bidens pilosa L. 被引量:7
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作者 田昕 周思祥 +5 位作者 魏荷琳 胡南 代志 刘志刚 韩正洲 屠鹏飞 《Journal of Chinese Pharmaceutical Sciences》 CAS 2011年第5期518-522,共5页
Chemical constituents of the whole herb of Bidens pilosa L. were investigated. By using various column chromatography (CC) and spectroscopic methods, 13 flavonoids were isolated and identified as follows: 2′-hydro... Chemical constituents of the whole herb of Bidens pilosa L. were investigated. By using various column chromatography (CC) and spectroscopic methods, 13 flavonoids were isolated and identified as follows: 2′-hydroxy-4,4′-dimethoxy-chalcone (1), isoliquiritigenin (2), 3,2′,4′-trihydroxy-4-methoxy-chalcone (3), licochalcone A (4),4′-O-β-D-glucopyranosyl-2′,3-dihydroxy-4-methoxy-chalcone (5), butein (6), apigenin (7), luteolin (8), diosmetin (9), chrysoeriol (10), 3,5,6,7,3′,4′,5′-heptamethoxyflavone (11), 7-O-β-D-glucopyranosyt-5,3′dihydroxy-3,6,4′-trimethoxyfiavone (12), sulfuretin (13). Compounds 1-5, 9, 11 were obtained from the genus Bidens for the first time. 展开更多
关键词 Bidens pilosa L. BIDENS COMPOSITAE Chemical constituents FLAVONOIDS CHALCONE FLAVONE
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Current plant diversity but not its soil legacy influences exotic plant invasion 被引量:3
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作者 Wei Xue Si-Mei Yao +3 位作者 Lin Huang Sergio RRoiloa Bao-Ming Ji Fei-Hai Yu 《Journal of Plant Ecology》 SCIE CSCD 2022年第3期639-649,共11页
Current plant diversity can influence exotic plant invasion,but it is unclear whether there is a legacy effect of plant diversity on exotic plant invasion.As plant diversity can affect soil microbial communities and p... Current plant diversity can influence exotic plant invasion,but it is unclear whether there is a legacy effect of plant diversity on exotic plant invasion.As plant diversity can affect soil microbial communities and physio-chemical properties,which may cascade to impact subsequent exotic plant growth,we hypothesize that the soil legacy effect of plant diversity can influence exotic plant invasion.We conducted a plant–soil feedback experiment.In the conditioning phase,we trained soils by monocultures of 12 plant species from three functional groups(4 grasses,3 legumes and 5 forbs)and mixtures of 8 randomly selected species with all three functional groups from this 12-species pool.In the test phase,we grew the invasive plant Bidens pilosa with a co-occurring native grass(Arthraxon hispidus),with a co-occurring native forb(Pterocypsela indica)or with both in each type of the conditioned soils.The performance of B.pilosa relative to its native competitors varied depending on the functional type of both conditioning plant species in the conditioning phase and competing plant species in the test phase.Diversity of the conditioning plants did not influence the growth difference between B.pilosa and its native competitors.However,increasing diversity of the competing plant species reduced the performance of B.pilosa relative to its native competitors.Our results suggest that current plant diversity can reduce exotic plant invasion through increasing growth inequality between invasive and native plants,but the soil legacy effect of plant diversity may have little impact on exotic plant invasion. 展开更多
关键词 Bidens pilosa competitive balance diversity effect invasive plant plant–soil feedback
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