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类胡萝卜素裂解酶催化不同底物生成非香气物质的条件及优化 被引量:3
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作者 朱明明 樊明涛 +1 位作者 何鸿举 马汉军 《食品工业科技》 CAS CSCD 北大核心 2016年第24期207-214,共8页
利用高效液相色谱研究类胡萝卜素裂解酶催化不同底物生成的非香气物质随酶解时间、p H、温度的变化规律,结果表明非香气物质生成量会随着酶解时间的增加、p H的降低和温度的升高出现先增加后减少的趋势。另外发现β-胡萝卜素在不同反应... 利用高效液相色谱研究类胡萝卜素裂解酶催化不同底物生成的非香气物质随酶解时间、p H、温度的变化规律,结果表明非香气物质生成量会随着酶解时间的增加、p H的降低和温度的升高出现先增加后减少的趋势。另外发现β-胡萝卜素在不同反应p H下可生成不同的裂解产物。利用二次多项式逐步回归得到酶解的最佳工艺条件,β-胡萝卜素、玉米黄质、β-阿朴-8'-胡萝卜醛、角黄质和虾青素的酶解条件分别为p H4.5、温度30℃、时间15 min,p H4.5、温度30℃、时间13 min,p H4.5、温度33℃、时间45 min,p H4.0、温度30℃、时间41 min,p H4.5、温度50℃、时间7 min。按上述条件进行酶解反应,得到产物β1、z1和z2、a1、c1和c2、x1和x2的生成量分别为定义条件下的1.74、1.80、1.39、1.66和1.75倍。该方法有效提高了非香气物质的生成量,并节约了时间。 展开更多
关键词 Β-胡萝卜素 β-阿朴-8’-胡萝卜醛 叶黄素类 胡萝卜素裂解酶 酶解因素 二次多项式逐步回归
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Changes in Violaxanthin Deepoxidase Activity and Unsaturation of Thylakoid Membrane Lipids in Indica and Japonica Rice Under Chilling Condition and Strong Light 被引量:1
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作者 季本华 曹云英 +3 位作者 谢焕松 朱素琴 马强 焦德茂 《Acta Botanica Sinica》 CSCD 2003年第9期1063-1070,共8页
To explore the differences of sensitivities to chilling and strong light in indica and japonica rice (Oryza sativa L), the changes in unsaturation of thylakoid membrane lipids and xanthophyll cycle were studied under ... To explore the differences of sensitivities to chilling and strong light in indica and japonica rice (Oryza sativa L), the changes in unsaturation of thylakoid membrane lipids and xanthophyll cycle were studied under chilling condition and strong light. The contents of unsaturated fatty acids of thylakoid membrane lipids decreased and that of the saturated ones increased with the time of chilling and strong light treatment, resulting in the reduction of the index of unsaturation of fatty acids (IUFA). The activity of violaxanthin deepoxidase (VDE), a key enzyme of xanthophyll cycle, also reduced. The content of violaxanthin (V) increased, and the contents of antheraxanthin (A) and zeaxanthin M decreased, the ratio of (A+Z)/ (A+Z+V) decreased correspondingly. Arrhenius analysis showed that VDE was sensitive to both chilling and unsaturation level of thylakoid membrane lipids. Correlation analysis showed that there was distinctly positive relationships between IUFA of thylakoid membrane lipids and the activity of VDE, Fv/Fm, and D, protein content. Lower IUFA values, less fluidity and stability of thylakoid membrane lipids, lower VDE activity and (A+Z)/(A+Z+V) ratio were found in indica rice cv. Shanyou 63 than in japonica rice cv. 9516 under chilling and strong light. 展开更多
关键词 thylakoid membrane lipids xanthophyll cycle violaxanthin deepoxidase Arrhenius plotting RICE
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Flavonoids from Leaves of Heritiera littoralis D. 被引量:17
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作者 TIANYan WUJun ZHANGSi 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第3期214-216,共3页
The mangrove plant Heritiera littoralis D. (Sterculi-aceae) is widelydistributed in southeastern Asia and traditionally used as a fish, arrowhead, and spearhead poison .It has also been used as a folk medicine to trea... The mangrove plant Heritiera littoralis D. (Sterculi-aceae) is widelydistributed in southeastern Asia and traditionally used as a fish, arrowhead, and spearhead poison .It has also been used as a folk medicine to treat he-maturia and diarrhea for a long history inChina . Past investigations on the chemical constituents of this plant have yielded fourtriterpenes, five sesquiterpenes, a phe-nylproanoid-glycerol and four flavonoids. In the presentstudy, eight flavonoids were isolated from the 95% EtOH extract of the leaves of this plant, andidentified as quercitrin (1), quercetin (2),kaempferol-3-O-(6″-O-E-p-coumaroyl)-β-D-glucopyranoside ( 3 ), kaempferol (4), kaempferitrin (5),myricetin (6), eriodictyol (7), and catechin (8) on the basis of their spectral data. 展开更多
关键词 heritiera littoralis D. STERCULIACEAE FLAVONOIDS
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