Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant g...Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.展开更多
Effects of the inlet guide vanes on the static characteristics, aerodynamic noise and internal flow characteristics of a small axial flow fan are studied in this work. The inlet guide vanes with different outlet angle...Effects of the inlet guide vanes on the static characteristics, aerodynamic noise and internal flow characteristics of a small axial flow fan are studied in this work. The inlet guide vanes with different outlet angle are designed, which are mounted on the casing and located at the upstream of the impeller of the prototype fan. Both steady and unsteady flow simulations arc performed. The steady flow is simulated by the calculations of Navier-Stokes equa- tions coupled with RNG k-epsilon turbulence model, while the unsteady flow is computed with large eddy simu- lation. According to the theoretical analysis, the inlet guide vanes with outlet angle of 60° are regarded as the op- timal inlet guide vanes. The static characteristic experiment is carried out in a standard test rig and the aerody- namic noise is tested in a semi-anechoic room. Then, performances of the fan with optimal inlet guide vanes are compared with those of the prototype fan. The results show that there is reasonable agreement between the simu- lation results and the experimental data. It is found that the static characteristics of small axial flow fan is im- proved obviously after installing the optimal inlet guide vanes. Meanwhile, the optimal inlet guide vanes have effect on reducing noise at the near field, but have little effect on the noise at the far field.展开更多
文摘Suaeda salsa L. seedlings grown in Hoagland nutrient solution were treated with different concentrations of NaCl combined with two levels of K + (12 μmol/L and 6 mmol/L) to study the K + nutrition effect on plant growth and leaf tonoplast V-H +-ATPase and V-H +-PPase activity. Increase of K + supply in the culture solution markedly increased the fresh weight, dry weight and K + content of S. salsa plants. Western blot analysis showed that the leaf V-H +-ATPase of S. salsa was at least composed of A,B,C,D,E and c subunits, and their expression decreased with the increase of NaCl concentration under K + starvation (12 μmol/L K +), but increased under normal K + application (6 mmol/L K +). Leaf V-H +-PPase molecular weight was about 72.6 kD and its expression increased as NaCl concentration increased under both high or low levels of K + concentration in nutrient solution. There was a positive correlation between of V-H +-ATPase or V-H +-PPase activity and the amounts of their expression. Results in this study suggest that K + nutrition plays an important role in the salt tolerance of S. salsa, and K + is involved in the regulation of V-H +-ATPase or V-H +-PPase activity under salt stress.
基金supported by National Natural Science Foundation of China No.51276172Public Welfare Technology Application Projects of Zhejiang Province NO.2015C31002Open Foundation of Zhejiang Provincial Top Key Academic Discipline of Mechanical Engineering and ZSTUMEOIA04 and 2013TD18
文摘Effects of the inlet guide vanes on the static characteristics, aerodynamic noise and internal flow characteristics of a small axial flow fan are studied in this work. The inlet guide vanes with different outlet angle are designed, which are mounted on the casing and located at the upstream of the impeller of the prototype fan. Both steady and unsteady flow simulations arc performed. The steady flow is simulated by the calculations of Navier-Stokes equa- tions coupled with RNG k-epsilon turbulence model, while the unsteady flow is computed with large eddy simu- lation. According to the theoretical analysis, the inlet guide vanes with outlet angle of 60° are regarded as the op- timal inlet guide vanes. The static characteristic experiment is carried out in a standard test rig and the aerody- namic noise is tested in a semi-anechoic room. Then, performances of the fan with optimal inlet guide vanes are compared with those of the prototype fan. The results show that there is reasonable agreement between the simu- lation results and the experimental data. It is found that the static characteristics of small axial flow fan is im- proved obviously after installing the optimal inlet guide vanes. Meanwhile, the optimal inlet guide vanes have effect on reducing noise at the near field, but have little effect on the noise at the far field.
