对排球普修课技术教学引入分层、合作相结合教学模式的实验研究

本文结合当前教育改革的最新理念,在传统教学的基础上提出分层、合作相结的教学模式,通过教学实验,对分层、合作相结合教学模式在排球普修课技术教学中的设计与运用进行了实践探索,实验证实本文提出的分层、合作相结合教学模式是合理的、可行的并富有成效的。结果分析证明分层、合作相结合教学模式比传统教学模式更有利于学生技术技能的掌握,并且能充分调动不同水平学生学习的积极性,增强学生的自信心,培养学生的合作精神和竞争意识,促进学生个性的发展和形成良好的社会适应能力。

创新创意服装设计教育教学与跨学科合作相结合的综合研究

2020年伊始,我们发现自己正越来越快地个世界变化的速度推向了我们想象中的未来——迭代的数字化时代,新的媒体传播方式,更快速的社交网络,地域性的经济和文化事件,全球自然环境问题和公共卫生问题,正在这个或近或远的未来悄悄影响着人民的生活和社会的变革。

自学与合作学习相结合的初中数学教学课堂

传统的数学教学,学生处于一种被动的学习状态,老师讲什么,学生就学什么,记什么,从来都不主动去学习和思考,学习效率比较低.随着新课程改革的不断推广,一种新的数学课堂模式,即自学与合作学习相结合的教学模式,逐渐得到了广大老师的认可.

校企合作背景下高职英语教学改革探索

在校企合作背景下,可从教学理念、师资、教学内容、教学方法和实践教学五方面入手,以实用为目的,以学生为中心,深化教学改革,建立与专业相结合的教学模式,提高高职英语教学实效性。

Computational Simulation of Aptamer-target Binding Mechanisms

Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as biochemical sensors for medicine,food safety and environmental monitoring.However,there is little research on aptamer-target binding mechanisms,which limits their application and development.Computational simulation has gained much attention for revealing aptamer-target binding mechanisms at the atomic level.This work summarizes the main simulation methods used in the mechanistic analysis of aptamer-target complexes,the characteristics of binding between aptamers and different targets(metal ions,small organic molecules,biomacromolecules,cells,bacteria and viruses),the types of aptamer-target interactions and the factors influencing their strength.It provides a reference for further use of simulations in understanding aptamer-target binding mechanisms.

Zonal Coupling Analysis Method of Seismic Response of Offshore Monopile Wind Turbine

The seismic safety of offshore wind turbines is an important issue that needs to be solved urgently.Based on a unified computing framework,this paper develops a set of seawater-seabed-wind turbine zoning coupling analysis methods.A 5 MW wind turbine and a site analysis model are established,and a seismic wave is selected to analyze the changes in the seismic response of offshore monopile wind turbines under the change of seawater depth,seabed wave velocity and seismic wave incidence angle.The analysis results show that when the seawater increases to a certain depth,the seismic response of the wind turbine increases.The shear wave velocity of the seabed affects the bending moment and displacement at the bottom of the tower.When the angle of incidence increases,the vertical displacement and the acceleration of the top of the tower increase in varying degrees.

Calculation of hydrogen solubility in molten alloys

A thermodynamic model was developed to calculate the hydrogen solubility in molten alloys based on the hydrogen solubility in constitutional pure liquid metals and their interaction parameters. The calculated results have a good agreement with the documented experimental results. The closer the molten alloy to an ideal liquid is, the more accurate the calculated results are. The compound forming ability and molar mixing heat of the constitutional elements take important roles in influencing the hydrogen solubility in molten alloys.

Analysis on Interaction between Genotype of Four Main Flavonoids of Barley Grain and Environment

[Objective] This study aimed to analyze the interaction between genotype of flavonoids of barley grain and environment, to increase the flavonoid content of barley grain in cultivation and breeding. [Method] In this study, the content of cate- chin, myricetin, quercetin and kaempferol of barley grain planted in Kunming, Qujing and Baoshan were determined by HPLC, and the genotype, environment, genotype- environment interaction of the flavonoid content of barley grain were analyzed. [Result] According to the experimental results, the genotype variance, environmental variance and G x E interaction variance of catechin and kaempferol contents show the same trend： genotype variation 〉 environmental variation 〉 G × E interaction variation, which all reach a extremely significant level; the genotype variance, envi- ronmental variance and G × E interaction variance of quercetin and total flavonoid contents show the same trend： genetype variation 〉 G × E interaction variation 〉 environmental variation, which all reach a extremely significant level; the genotype variance and environmental variance of myricetin content both reach a extremely sig- nificant level, while the G × E interaction variance reaches a significant level, showing an order of genotype variation 〉 environmental variation 〉 G × E interaction variation; the genotype variance, environmental variance and G x E interaction vari- ance of total flavonoid content show an order of genotype variation 〉 environmental variation 〉 G × E interaction variation. Among different barley varieties, Ziguang- mangluoerling and Kuanyingdamai in Qujing, Kunming and Baoshan have relatively high content of quercetin, while other barley varieties barely contain any quercetin. The grains of Ziguangmangluoerling and Kuanyingdamai are purple, while the grains of other barley varieties are yellow. [Conclusion] Four main flavonoids and the total flavonoids of barley grain are mainly under genetic control and affected by genetic- environment interactions; the purple barley grains contain high content of quercetin.

Preparation, characterization, and catalytic performance of high efficient CeO_2-MnO_x-Al_2O_3 catalysts for NO elimination

A series of CeO2‐MnOx‐Al2O3 mixed oxide catalysts （Ce：Mn：Al mole ratio=6：4：x, x=0.25, 0.5, 1, 2） were prepared by a simple one‐step inverse co‐precipitation method to investigate the influence of the incorporation of Al3＋ into CeO2‐MnOx mixed oxides. CeO2‐MnOx, CeO2‐Al2O3, and MnOx‐Al2O3 mixed oxides, and CeO2 were prepared by the same method for comparison. The samples were characterized by XRD, Raman, N2 physisorption, H2‐TPR, XPS, and in situ DRIFTS. The catalytic re‐duction of NO by CO was chosen as a model reaction to evaluate the catalytic performance. The incorporation of a small amount of Al3＋into CeO2‐MnOx mixed oxides resulted in a decrease of crys‐tallite size, with the increase of the BET specific surface area and pore volume, as well as the in‐crease of Ce3＋and Mn4＋. The former benefits good contact between catalyst and reactants, and the latter promotes the adsorption of CO and the desorption, conversion and dissociation of adsorbed NO. All these enhanced the catalytic performance for the NO＋CO model reaction. A reaction mecha‐nism was proposed to explain the excellent catalytic performance of CeO2‐MnOx‐Al2O3 catalysts for NO reduction by CO.

XPS AND UPS STUDIES OF THE INTERFACIAL INTERACTION IN Ni ZrO 2 COMPOSITE PLATING

The interfacial interaction existing in the Ni ZrO 2 composite plating has been investigated. The experimental results show that no new phases were formed in the interfacial regions between matrix Ni and ZrO 2 particles, but an orbital interaction through the mutual overlap of the d orbits does exist in the interfacial regions between Ni atoms and Zr 3+ ions.

幼儿园科学课程实践研究

随着《纲要》的颁布,新的教育观念冲击着传统的幼儿园教育,改革传统的幼儿园课程已势在必行,我们幼儿园也深知课改的重要性,慢慢地也走进了课改的行列,我们通过请专家教授亲自来园指挥讲座,理论学习,实地学习等多咱渠道,使教师不断领会“课改”精神,回归生活、关注过程、加强整合、注重选择作为二期课改新教材的主要特点,凸显了一切以幼儿发展为本的宗旨,更引领着我们教师转变观念、突破创新.通过学习,我们积极探索与实践,在课改背景下的幼儿园科学课程.实践的过程是我们对已有的园本课程再创造与再构建的过程,同时也促使了教师们专业的成长.

Interactions between γ-TiAl melt and Y_2O_3 ceramic material during directional solidification process

A γ-TiAl alloy with nominal composition of Ti-47%Al（molar fraction） was directionally solidified in an alumina mould with an Y2O3 protective coating.The effects of processing parameters（melting temperature and interaction time） on the metal-coating interface,microstructure and chemical composition of the alloy were evaluated.The result shows that the Y2O3 protective coating exhibits an effective barrier capability to avoid direct contact between the mould base material and the TiAl melt,although the Y2O3 coating is found to suffer some erosion and be slightly dissolved by the molten TiAl due to the coating-metal interactions.The directionally solidified alloys were contaminated with Y and O,and Y2O3 inclusions were dispersed in the metal matrix.The reason for this metal contamination is the Y2O3 coating dissolution by the TiAl melt.One mode of the interaction between Y2O3 and the TiAl melt is dissolution of yttrium and atomic oxygen in the melt by reaction Y2O3（s）=2Y（in TiAl melt）＋3O（in TiAl melt）.Both the extent of alloy contamination and the volume fractions of Y2O3 inclusions depend on the melting temperature and the interaction time.

A Global ab initio Potential Energy Surface for F+H_(2)→HF+H

A global three dimensional potential energy surface for the F＋H2→HF＋H reaction has been developed by spline interpolation of about 15,000 symmetry-unique ab initio points, obtained from the multi-reference configuration interaction level with Davidson correction using the aug-cc-pV5Z basis set. In the entrance channel the spin-orbit coupling energy is also included.

Limited Feedback Bit Allocation for Cooperative Multi-cell Systems with Multi- user MIMO

For the cooperative multi-cell systems with muki-user MIMO, a new limited feedback bit allocation scheme is proposed to minimize the rate loss caused by quantization error. In the proposed scheme, the Channel State Information （CSI） feedback of cell-edge user for the local service cell and the adjacent interference cell are separately quantized. Based on the upper bound of the rate loss of cell-edge user due to the limited feedback, the number of feedback bits for quantized CSI of the local service cell and the adjacent cell are optimized with the fixed total bits of the limited feedback. The simulation shows that our proposed scheme of feedback bits allocation efficiently decreases the interference and increases the rate of systems compared with that of equal bits allocation and those of other allocations.

The BLOC Interactomes Form a Network in Endosomal Transport

With the identification of more than a dozen novel Hermansky-Pudlak Syndrome （HPS） proteins in vesicle trafficking in higher eukaryotes, a new class of trafficking pathways has been described. It mainly consists of three newly-defined protein com- plexes, BLOC-l, -2, and -3. Compelling evidence indicates that these complexes together with two other well-known complexes, AP3 and HOPS, play important roles in endosomal transport. The interactions between these complexes form a network in protein trafficking via endosomes and cytoskeleton. Each node of this network has intra-complex and extra-complex interactions. These complexes are connected by direct interactions between the subunits from different complexes or by indirect interactions through coupling nodes that interact with two or more subunits from different complexes. The dissection of this network facilitates the understanding of a dynamic but elaborate transport machinery in protein/membrane trafficking. The disruption of this network may lead to abnormal trafficking or defective organellar development as described in patients with Hermansky-Pudlak syndrome.

Hexanuclear ring cobalt complex for photochemical CO_(2) to CO conversion

Photosynthesis in nature has been deemed as the most significant biochemical reaction,which maintains a relatively stable content of O_(2) and CO_(2) in the atmosphere.Herein,for a deeper comprehension of natural photosynthesis,an artificial photosynthesis model reaction of photochemical CO_(2) to CO conversion(CO_(2)+2 H^(+)+2e^(-)→CO+H_(2)O)catalyzed by a homogeneous hexanuclear ring cobalt complex{K_(2)[CoO_(3)PCH_(2)N(CH_(2)CO_(2))_(2)]}_(6)(Co6 complex)is developed.Using the[Ru(bpy)_(3)]^(2+)as a photosensitizer and TEOA as a sacrificial electron donor,an optimal turnover frequency of 503.3 h^(‒1) and an apparent quantum efficiency of 0.81%are obtained.The good photocatalytic CO_(2) reduction performance is attributed to the efficient electron transfer between Co6 complex and[Ru(bpy)_(3)]^(2+),which boosts the photogenerated carriers separation of the photosensitizer.It is confirmed by the j‐V curves,light‐assisted UV‐vis curves,steady‐state photoluminescence spectra and real‐time laser flash photolysis experiments.In addition,the proposed catalytic mechanism for CO_(2) reduction reaction catalyzed by the Co6 complex is explored by the potassium thiocyanate poison experiment,Pourbaix diagram and density functional theory calculations.

Finite Range Effects on Fusion and/or Breakup of ~6He+^(238)U and ^(11)Li+^(208)Pb Systems

We have studied the effects of the finite range on the fusion and/or breakup of ^6He^＋238U and ^11Li＋^208pb Pb dynamic polarization potential approach. It has been found of the interaction between the fragments of the projectile systems at near barrier energies within the framework of that at near barrier energies the maximum flux is lost to the breakup channel and at energies well above the Coulomb barrier the fusion coupled with the breakup channel opens up, initially with sharp rise and later becoming saturated at energy nearly twice of the Coulomb barrier. Further, it is found that the breakup cross section increases with the increasing range of the interaction between the fragments of the projectile while the fusion coupled with the breakup channel cross section decreases with the increasing range.

Coupling effects of influence factors on probability of corrosion initiation time of reinforced concrete

The coupling model of major influence factors such state affecting the chloride diffusion process in concrete is as environmental relative humidity, load-induced crack and stress discussed. The probability distributions of the critical chloride concentration Cc, the chloride diffusion coefficient D, and the surface chloride concentration Cs were determined based on the collected natural exposure data. And the estimation of probability of corrosion initiation considering the coupling effects of influence factors is presented. It is found that the relative humidity and curing time are the most effective factors affecting the probability of corrosion initiation before and after 10 years of exposure time. At the same exposure time, the influence of load-induced crack and stress state on the probability of corrosion initiation is obvious, in which the effect of crack is the most one

Theoretical Investigating Mechanisms of Drug-Resistance Generated by Mutation-Induced Changes in Influenza Viruses

Influenza A(A/H_(x)N_(y))is a significant public health concern due to its high infectiousness and mortality.Neuraminidase,which interacts with sialic acid(SIA)in host cells,has become an essential target since its highly conserved catalytic center structure,while resistance mutations have already generated.Here,a detailed investigation of the drug resistance mechanism caused by mutations was performed for subtype N9(A/H7N9).Molecular dynamics simulation and alanine-scanning-interaction-entropy method(ASIE)were used to explore the critical differences between N9 and Zanamivir(ZMR)before and after R294K mutation.The results showed that the mutation caused the hydrogen bond between Arg294 and ZMR to break,then the hydrogen bonding network was disrupted,leading to weakened binding ability and resistance.While in wild type(A/H7N9^(WT)),this hydrogen bond was initially stable.Meanwhile,N9 derived from A/H11N9 was obtained as an R292K mutation.Then the relative binding free energy of N9 with five inhibitors(SIA,DAN,ZMR,G28,and G39)was predicted,basically consistent with experimental values,indicating that the calculated results were reliable by ASIE.In addition,Arg292 and Tyr406 were hot spots in the A/H11N9^(WT)-drugs.However,Lys292 was not observed as a favorable contributing residue in A/H11N9^(R292K),which may promote resistance.In comparison,Tyr406 remained the hotspot feature when SIA,ZMR,and G28 binding to A/H11N9^(R292K).Combining the two groups,we speculate that the resistance was mainly caused by the disruption of the hydrogen bonding network and the transformation of hotspots.This study could guide novel drug delivery of drug-resistant mutations in the treatment of A/H_(x)N9.

Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer

AIM TO uncover the roles of tumor-promoting gene ZEB1 in aerobic glycolysis regulation and shed light on the underlying molecular mechanism.METHODS Endogenous zinc finger E-box binding homeobox-1 （ZEB1） was silenced using a and the impact of ZEB1 and lentivirus-mediated method, methyI-CpG binding domain protein 1 （MBD1） on aerobic glycolysis was measured using seahorse cellular flux analyzers, reactive oxygen species quantification, and mitochondrial membrane potential measurement. The interaction between ZEB1 and MBD1 was assessed by co-immunoprecipitation and immunofluorescence assays. The impact of ZEB1 and MBD1 interaction on sirtuin 3 （SIRT3） expression was confirmed by quantitative polymerase chain reaction, western blotting, and dual-luciferase and chromatinimmunoprecipitation assays.RESULTS ZEB1 was a positive regulator of aerobic glycolysis in pancreatic cancer. ZEB1 transcriptionally silenced expression of SIRT3, a mitochondrial-localized tumor suppressor, through interaction with MBD1.CONCLUSION ZEB1 silenced SIRT3 expression via interaction with MBD1 to promote aerobic glycolysis in pancreatic cancer.