Reverse transcription quantitative PCR （RT-qPCR） combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including Studying the regulation of spi...Reverse transcription quantitative PCR （RT-qPCR） combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including Studying the regulation of spinosyn biosynthesis, finding new target genes for engineering, and discovering and exploiting other macrolide secondary metabolites. Studies have demonstrated that appropriate internal control is needed to normalize target genes at transcription levels. However, many studies have shown that no single reference gene is universal for all strains under all experimental conditions. Thus, eight candidate reference genes of three different S. spinosa strains in two different cultures were studied to find suitable reference gene（sl. The number of amplification cycles of these candidate genes was calculated by BestKeeper, NormFinder and geNorm. The results indicated that the most suitable reference genes for normalization during the fermentation of S. spinosa were 16S rRNA and rbL13.展开更多
Plants produce a wide spectrum of secondary metabolites that play critical roles in plant-environment interactions and against biotic and abiotic stresses. Moreover, many secondary metabolites have pharmaceu- tical ef...Plants produce a wide spectrum of secondary metabolites that play critical roles in plant-environment interactions and against biotic and abiotic stresses. Moreover, many secondary metabolites have pharmaceu- tical efficacy for a wide range of diseases （cancer, malaria, etc.）. Controlled transcription of biosynthetic genes is one of the major mechanisms regulating sec- ondary metabolism in plants. Several transcription factor families such as MYC, MYB, WRKY and AP2/ERF have been found to be involved in the regulation of secondary metabolism in different medicinal plants. In addition, the biosynthesis and proper accumulation of secondary metabolites are also induced by signaling molecule jasmonic acid （JA）. This review provides an insight into JA signaling pathway and JA-mediated transcriptional regu- lation of secondary metabolism （vinblastine, nicotine, artemisinin, taxol and ginsenoside） in a range of medicinal plant species.展开更多
基金Supported by the National Natural Science Foundation of China(No.21076148 and 31270087)Plan for Tianjin Science and Technology Support(No.11ZCKFSY0100)
文摘Reverse transcription quantitative PCR （RT-qPCR） combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including Studying the regulation of spinosyn biosynthesis, finding new target genes for engineering, and discovering and exploiting other macrolide secondary metabolites. Studies have demonstrated that appropriate internal control is needed to normalize target genes at transcription levels. However, many studies have shown that no single reference gene is universal for all strains under all experimental conditions. Thus, eight candidate reference genes of three different S. spinosa strains in two different cultures were studied to find suitable reference gene（sl. The number of amplification cycles of these candidate genes was calculated by BestKeeper, NormFinder and geNorm. The results indicated that the most suitable reference genes for normalization during the fermentation of S. spinosa were 16S rRNA and rbL13.
基金This work was supported by the National Natural Science Foundation of China （30470189, 81071821, 81250110086 and 81250110551）, the Fundamental Research for the Central University of China （721500011）, the Key Hunan Provincial Natural Science Foundation of China （13112016 and 07JJ5096）, the Science and Technology Program of Hunan Province of China （2014SK4050） and Changsha National High Tech Zone Innovation Leading Talent Program （2014-37）.
文摘Plants produce a wide spectrum of secondary metabolites that play critical roles in plant-environment interactions and against biotic and abiotic stresses. Moreover, many secondary metabolites have pharmaceu- tical efficacy for a wide range of diseases （cancer, malaria, etc.）. Controlled transcription of biosynthetic genes is one of the major mechanisms regulating sec- ondary metabolism in plants. Several transcription factor families such as MYC, MYB, WRKY and AP2/ERF have been found to be involved in the regulation of secondary metabolism in different medicinal plants. In addition, the biosynthesis and proper accumulation of secondary metabolites are also induced by signaling molecule jasmonic acid （JA）. This review provides an insight into JA signaling pathway and JA-mediated transcriptional regu- lation of secondary metabolism （vinblastine, nicotine, artemisinin, taxol and ginsenoside） in a range of medicinal plant species.
