The endogenous hydrogen sulfide (H2S), as a new gasotransmitter, participates in many plant physio- logical processes. In this study, we found that both ethylene and drought strongly induced HaS synthetase AtD-CDes ...The endogenous hydrogen sulfide (H2S), as a new gasotransmitter, participates in many plant physio- logical processes. In this study, we found that both ethylene and drought strongly induced HaS synthetase AtD-CDes gene expression and enzymatic activity. H2S synthesis inhibitors restrained the ethylene and drought induced stomatal closure in Arabidopsis. The H2S synthetase mutant Atd-cdes was insensitive to ethylene and drought, and overexpression of AtD-CDes conferred the transgenic plants more sensitive to ethylene and drought. Sequence analysis of AtD-CDes promoter showed that it contained ethylene response cis element ERE, and abiotic stress responsive cis elements MBS, LTR, and ABRE. The AtD- CDes promoter fused with GUS was transformed into Arabidopsis thaliana to get AtD-CDes promoter::GUS transgenic plants. When treated with ethylene and drought stress, the enzymatic activity of fl-glucuronidase was higher in the leaves and stomata of transgenic Arabidopsis. Analyses of GUS activity from the transgenic plants har- boring different fragments of the promoter shows that the key section of AtD-CDes promoter response to ethylene was from the -697 to -408 bp, and the key section response to drought stress was from -90 to -1 bp. These results suggested that the H2S produced from AtD-CDesmay mediate ethylene and drought-induced stomatal movement.展开更多
This paper presents results on the combustion of syngas fuel in re-circulating vortex combustor. The combustion stability is achieved through the use of cavities in which recirculation zones of hot products generated ...This paper presents results on the combustion of syngas fuel in re-circulating vortex combustor. The combustion stability is achieved through the use of cavities in which recirculation zones of hot products generated by the direct injection of fuel and air are created and acting as a continuous source of ignition for the incoming main fuel-air stream. CFD (computational fluid dynamics) analysis was performed in this study to test the combustion performance and emissions from the vortex trapped combustor using synthetic gas or syngas fuel produced from the gasification process. The flame temperature, the flow field and species concentrations inside the vortex trapped combustor were obtained. Several syngas fuels with different fuel compositions (H2, CO, CH4, CO2, N2 and H20) and lower heating values were tested in this study. The changes on the flame temperature and species concentrations inside the combustor, the emissions of NOx, CO, CO2 at the exit of the combustor, the combustor efficiency and the total pressure drop for syngas fuels are presented in this paper. The effect of H2/CO ratio and the mass fraction of each constituent of syngas fuels and hydrogen-methane fuel mixtures on the combustion and emissions performances were investigated.展开更多
基金supported by the National Natural Science Foundation of China (31170237, 31572107, 31401844, 31540090)
文摘The endogenous hydrogen sulfide (H2S), as a new gasotransmitter, participates in many plant physio- logical processes. In this study, we found that both ethylene and drought strongly induced HaS synthetase AtD-CDes gene expression and enzymatic activity. H2S synthesis inhibitors restrained the ethylene and drought induced stomatal closure in Arabidopsis. The H2S synthetase mutant Atd-cdes was insensitive to ethylene and drought, and overexpression of AtD-CDes conferred the transgenic plants more sensitive to ethylene and drought. Sequence analysis of AtD-CDes promoter showed that it contained ethylene response cis element ERE, and abiotic stress responsive cis elements MBS, LTR, and ABRE. The AtD- CDes promoter fused with GUS was transformed into Arabidopsis thaliana to get AtD-CDes promoter::GUS transgenic plants. When treated with ethylene and drought stress, the enzymatic activity of fl-glucuronidase was higher in the leaves and stomata of transgenic Arabidopsis. Analyses of GUS activity from the transgenic plants har- boring different fragments of the promoter shows that the key section of AtD-CDes promoter response to ethylene was from the -697 to -408 bp, and the key section response to drought stress was from -90 to -1 bp. These results suggested that the H2S produced from AtD-CDesmay mediate ethylene and drought-induced stomatal movement.
文摘This paper presents results on the combustion of syngas fuel in re-circulating vortex combustor. The combustion stability is achieved through the use of cavities in which recirculation zones of hot products generated by the direct injection of fuel and air are created and acting as a continuous source of ignition for the incoming main fuel-air stream. CFD (computational fluid dynamics) analysis was performed in this study to test the combustion performance and emissions from the vortex trapped combustor using synthetic gas or syngas fuel produced from the gasification process. The flame temperature, the flow field and species concentrations inside the vortex trapped combustor were obtained. Several syngas fuels with different fuel compositions (H2, CO, CH4, CO2, N2 and H20) and lower heating values were tested in this study. The changes on the flame temperature and species concentrations inside the combustor, the emissions of NOx, CO, CO2 at the exit of the combustor, the combustor efficiency and the total pressure drop for syngas fuels are presented in this paper. The effect of H2/CO ratio and the mass fraction of each constituent of syngas fuels and hydrogen-methane fuel mixtures on the combustion and emissions performances were investigated.