Matrix metalloproteinases(MMPs) are a large family of proteolytic enzymes which are involved in degradation of many different components of the extra cellular matrix.In this paper,the active center peptide domain of M...Matrix metalloproteinases(MMPs) are a large family of proteolytic enzymes which are involved in degradation of many different components of the extra cellular matrix.In this paper,the active center peptide domain of MMP-11 was synthesized by Fmoc-solid-phase synthesis method and purified by reversed-phase high performance liquid chromatography.The peptide was characterized by ESI-MS and 1H NMR.展开更多
A method for preparation of particle crystal film constructed from monodisperse silica colloidal particles in diameter of about 300 nm is reported. The films were prepared from an ethanol suspension by vertical deposi...A method for preparation of particle crystal film constructed from monodisperse silica colloidal particles in diameter of about 300 nm is reported. The films were prepared from an ethanol suspension by vertical deposition that relies on capillary forces to assemble colloidal crystal particles on a vertical substrate. The 3D ordered films were characterized by transmission spectra and scanning electric microscope (SEM). The effect of evaporation temperature, particle concentration and sintered temperature on the quality of colloidal particle crystal film was investigated.展开更多
Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes ...Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes in their living environment may be a sharp decrease in salt concentration. It is established that sulfated polysaccharides interact with hundreds of signaling molecules and facilitate many signaling transduction pathways, including fibroblast growth factor （FGF） and FGF receptor signaling pathway. The disappearance of sulfated polysaccharides in fimgi and plants on land might indicate that polysaccharides without sulfation might be sufficient in facilitating protein ligand/receptor interactions in low salinity land. Recently, it was reported that plants on land start to synthesize sulfated polysaccharides in high salt environment, suggesting that fungi might be able to do the same when ex- posed in such environment. Interestingly, Cordyceps, a fungus habituating inside caterpillar body, is the most valued traditional Chi- nese Medicine. One of the important pharmaceutical active ingredients in Cordyceps is polysaccharides. Therefore, we hypothesize that the salty environment inside caterpillar body might allow the fungi to synthesize sulfated polysaccharides. To test the hypothesis, we isolated polysaccharides from both lava and sporophore of wild Cordyceps and also from Cordyceps militaris cultured without or with added salts. We then measured the polysaccharide activity using a FGF2/FGFRlc signaling-dependent BaF3 cell proliferation assay and found that polysaccharides isolated from wild Cordyceps activated FGF2/FGFR signaling, indicating that the polysaccha- rides synthesized by wild Cordyceps are indeed different from those by the cultured mycelium.展开更多
AIM:To investigate whether human acyl-CoA synthetase 5(ACSL5) is sensitive to the ACSL inhibitor triacsin C.METHODS:The ACSL isoforms ACSL1 and ACSL5 from rat as well as human ACSL5 were cloned and recombinantly expre...AIM:To investigate whether human acyl-CoA synthetase 5(ACSL5) is sensitive to the ACSL inhibitor triacsin C.METHODS:The ACSL isoforms ACSL1 and ACSL5 from rat as well as human ACSL5 were cloned and recombinantly expressed as 6xHis-tagged enzymes.Ni 2+-affinity purified recombinant enzymes were assayed at pH 7.5 or pH 9.5 in the presence or absence of triacsin C.In addition,ACSL5 transfected CaCo2 cells and intestinal human mucosa were monitored.ACSL5 expression in cellular systems was verified using Western blot and immunofluorescence.The ACSL assay mix included TrisHCl(pH 7.4),ATP,CoA,EDTA,DTT,MgCl 2,[9,103 H] palmitic acid,and triton X-100.The 200 μL reaction was initiated with the addition of solubilized,purified recombinant proteins or cellular lysates.Reactions were terminated after 10,30 or 60 min of incubation with Doles medium.RESULTS:Expression of soluble recombinant ACSL proteins was found after incubation with isopropyl betaD-1-thiogalactopyranoside and after ultracentrifugation these were further purified to near homogeneity with Ni 2+-affinity chromatography.Triacsin C selectively and strongly inhibited recombinant human ACSL5 protein at pH 7.5 and pH 9.5,as well as recombinant rat ACSL1(sensitive control),but not recombinant rat ACSL5(insensitive control).The IC50 for human ACSL5 was about 10 μmol/L.The inhibitory triacsin C effect was similar for different incubation times(10,30 and 60 min) and was not modified by the N-or C-terminal location of the 6xHis-tag.In order to evaluate ACSL5 sensitivity to triacsin C in a cellular environment,stable human ACSL5 CaCo2 transfectants and mechanically dissected normal human intestinal mucosa with high physiological expression of ACSL5 were analyzed.In both models,ACSL5 peak activity was found at pH 7.5 and pH 9.5,corresponding to the properties of recombinant human ACSL5 protein.In the presence of triacsin C(25 μmol/L),total ACSL activity was dramatically diminished in human ACSL5 transfectants as well as in ACSL5-rich human intestinal mucosa.CONCLUSION:The data strongly indicate that human ACSL5 is sensitive to triacsin C and does not compensate for other triacsin C-sensitive ACSL isoforms.展开更多
The title compound Cu3PSe4 was synthesized by the reaction of CuCl, P2Se5 and Se in a molar ratio of 1:1:1 at 500 C and structurally characterized by X-ray crystallography. The crystal belongs to orthorhombic, space g...The title compound Cu3PSe4 was synthesized by the reaction of CuCl, P2Se5 and Se in a molar ratio of 1:1:1 at 500 C and structurally characterized by X-ray crystallography. The crystal belongs to orthorhombic, space group Pmn21 with cell parameters: a = 7.685(2), b = 6.656(1), c = 6.377(1) , V = 326.2(1) 3, Z = 2, Dc = 5.472 g/cm3, Mr = 537.43, F(000) = 476, m = 32.12 mm-1, R = 0.0642, wR = 0.1481 and S = 1.037. The 3-D structure can be regarded as constructed from the alternately stacking of [Cu(2)Se4] tetrahedral layers and Cu(1)PSe tetrahedral layers along the b direction, in which the Cu(2)Se layer is comprised of corner-sharing [Cu(2)Se4] tetrahedra along the a and c directions, and the Cu(1)PSe layer is consisted of alternately corner-sharing [Cu(1)Se4] tetrahedra and [PSe4] tetrahedra along the a and c directions.展开更多
The title compound was prepared from the corresponding elements(Sm, Te) using iodine as transport agent. The crystal structure was determined by the single crystal X-ray diffraction techniques with the crystal data as...The title compound was prepared from the corresponding elements(Sm, Te) using iodine as transport agent. The crystal structure was determined by the single crystal X-ray diffraction techniques with the crystal data as follows: SmTe3,M_r= 533. 15, orthorhombic. Cmcm, a=4. 323(2), b= 25. 613(3),c=4. 335(2) A,V=480. 0 A￣3, Z=4, D_x=7. 38 g/cm￣3, F(000) =872,μ= 300. 2 cm￣(-1), R=0. 061,Rw.=0. 066 for 660 observed(I≥3σ(I)) unique reflections. The compound is isostructural with NdTe_3￣[1].展开更多
We demonstrate the hydrothermal synthesis of long copper nanowires based on a simple protocol. We show that the purification of the nanowires is very important and can be achieved easily by wet treatment with glacial ...We demonstrate the hydrothermal synthesis of long copper nanowires based on a simple protocol. We show that the purification of the nanowires is very important and can be achieved easily by wet treatment with glacial acetic acid. Fabrication of random networks of purified copper nanowires leads to flexible transparent electrodes with excellent optoelectronic performances （e.g., 55 Ω/sq. at 94% transparency）. The process is carried out at room temperature and no post-treatment is necessary. Hybrid materials with the conductive polymer PEDOT：PSS show similar properties （e.g., 46 Ω/sq, at 93% transparency）, with improved mechanical properties. Both electrodes were integrated in capacitive touch sensors.展开更多
A new method of enantioselective synthesis of （S,S）-2,8-diazobicyclo [4.3.0] nonane was found by using （R）-2-amino-2- phenyl-ethanol as chiral induction reagent. The entire synthetic process included 8 steps which...A new method of enantioselective synthesis of （S,S）-2,8-diazobicyclo [4.3.0] nonane was found by using （R）-2-amino-2- phenyl-ethanol as chiral induction reagent. The entire synthetic process included 8 steps which were easy to operate with high yield. The purification method was only simple recrystallization or even used directly in the next step without further purifica- tion. The total yield was 29%.展开更多
文摘Matrix metalloproteinases(MMPs) are a large family of proteolytic enzymes which are involved in degradation of many different components of the extra cellular matrix.In this paper,the active center peptide domain of MMP-11 was synthesized by Fmoc-solid-phase synthesis method and purified by reversed-phase high performance liquid chromatography.The peptide was characterized by ESI-MS and 1H NMR.
基金Supported by the Chinese National Key Basic Research Special Fund (No.2001CB6104) and the National Natural Science Foundation of China(No.20076027)
文摘A method for preparation of particle crystal film constructed from monodisperse silica colloidal particles in diameter of about 300 nm is reported. The films were prepared from an ethanol suspension by vertical deposition that relies on capillary forces to assemble colloidal crystal particles on a vertical substrate. The 3D ordered films were characterized by transmission spectra and scanning electric microscope (SEM). The effect of evaporation temperature, particle concentration and sintered temperature on the quality of colloidal particle crystal film was investigated.
基金supported by the National Natural Science Foundation of China (Grant No. 91129706)
文摘Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes in their living environment may be a sharp decrease in salt concentration. It is established that sulfated polysaccharides interact with hundreds of signaling molecules and facilitate many signaling transduction pathways, including fibroblast growth factor （FGF） and FGF receptor signaling pathway. The disappearance of sulfated polysaccharides in fimgi and plants on land might indicate that polysaccharides without sulfation might be sufficient in facilitating protein ligand/receptor interactions in low salinity land. Recently, it was reported that plants on land start to synthesize sulfated polysaccharides in high salt environment, suggesting that fungi might be able to do the same when ex- posed in such environment. Interestingly, Cordyceps, a fungus habituating inside caterpillar body, is the most valued traditional Chi- nese Medicine. One of the important pharmaceutical active ingredients in Cordyceps is polysaccharides. Therefore, we hypothesize that the salty environment inside caterpillar body might allow the fungi to synthesize sulfated polysaccharides. To test the hypothesis, we isolated polysaccharides from both lava and sporophore of wild Cordyceps and also from Cordyceps militaris cultured without or with added salts. We then measured the polysaccharide activity using a FGF2/FGFRlc signaling-dependent BaF3 cell proliferation assay and found that polysaccharides isolated from wild Cordyceps activated FGF2/FGFR signaling, indicating that the polysaccha- rides synthesized by wild Cordyceps are indeed different from those by the cultured mycelium.
基金Supported by Deutsche Forschungsgemeinschaft, No. GA785/6-1Deutsche Krebshilfe, No. 109313the Rotationsprogramm of the Medical Faculty RWTH Aachen University (to Kaemmerer E)
文摘AIM:To investigate whether human acyl-CoA synthetase 5(ACSL5) is sensitive to the ACSL inhibitor triacsin C.METHODS:The ACSL isoforms ACSL1 and ACSL5 from rat as well as human ACSL5 were cloned and recombinantly expressed as 6xHis-tagged enzymes.Ni 2+-affinity purified recombinant enzymes were assayed at pH 7.5 or pH 9.5 in the presence or absence of triacsin C.In addition,ACSL5 transfected CaCo2 cells and intestinal human mucosa were monitored.ACSL5 expression in cellular systems was verified using Western blot and immunofluorescence.The ACSL assay mix included TrisHCl(pH 7.4),ATP,CoA,EDTA,DTT,MgCl 2,[9,103 H] palmitic acid,and triton X-100.The 200 μL reaction was initiated with the addition of solubilized,purified recombinant proteins or cellular lysates.Reactions were terminated after 10,30 or 60 min of incubation with Doles medium.RESULTS:Expression of soluble recombinant ACSL proteins was found after incubation with isopropyl betaD-1-thiogalactopyranoside and after ultracentrifugation these were further purified to near homogeneity with Ni 2+-affinity chromatography.Triacsin C selectively and strongly inhibited recombinant human ACSL5 protein at pH 7.5 and pH 9.5,as well as recombinant rat ACSL1(sensitive control),but not recombinant rat ACSL5(insensitive control).The IC50 for human ACSL5 was about 10 μmol/L.The inhibitory triacsin C effect was similar for different incubation times(10,30 and 60 min) and was not modified by the N-or C-terminal location of the 6xHis-tag.In order to evaluate ACSL5 sensitivity to triacsin C in a cellular environment,stable human ACSL5 CaCo2 transfectants and mechanically dissected normal human intestinal mucosa with high physiological expression of ACSL5 were analyzed.In both models,ACSL5 peak activity was found at pH 7.5 and pH 9.5,corresponding to the properties of recombinant human ACSL5 protein.In the presence of triacsin C(25 μmol/L),total ACSL activity was dramatically diminished in human ACSL5 transfectants as well as in ACSL5-rich human intestinal mucosa.CONCLUSION:The data strongly indicate that human ACSL5 is sensitive to triacsin C and does not compensate for other triacsin C-sensitive ACSL isoforms.
基金This work was supported by the National Natural Science Foundation of China (20001007 20131020) Natural Science Foundation of the Chinese Academy of Sciences (KJCX2-H3) and Fujian Province (2000F006)
文摘The title compound Cu3PSe4 was synthesized by the reaction of CuCl, P2Se5 and Se in a molar ratio of 1:1:1 at 500 C and structurally characterized by X-ray crystallography. The crystal belongs to orthorhombic, space group Pmn21 with cell parameters: a = 7.685(2), b = 6.656(1), c = 6.377(1) , V = 326.2(1) 3, Z = 2, Dc = 5.472 g/cm3, Mr = 537.43, F(000) = 476, m = 32.12 mm-1, R = 0.0642, wR = 0.1481 and S = 1.037. The 3-D structure can be regarded as constructed from the alternately stacking of [Cu(2)Se4] tetrahedral layers and Cu(1)PSe tetrahedral layers along the b direction, in which the Cu(2)Se layer is comprised of corner-sharing [Cu(2)Se4] tetrahedra along the a and c directions, and the Cu(1)PSe layer is consisted of alternately corner-sharing [Cu(1)Se4] tetrahedra and [PSe4] tetrahedra along the a and c directions.
文摘The title compound was prepared from the corresponding elements(Sm, Te) using iodine as transport agent. The crystal structure was determined by the single crystal X-ray diffraction techniques with the crystal data as follows: SmTe3,M_r= 533. 15, orthorhombic. Cmcm, a=4. 323(2), b= 25. 613(3),c=4. 335(2) A,V=480. 0 A￣3, Z=4, D_x=7. 38 g/cm￣3, F(000) =872,μ= 300. 2 cm￣(-1), R=0. 061,Rw.=0. 066 for 660 observed(I≥3σ(I)) unique reflections. The compound is isostructural with NdTe_3￣[1].
文摘We demonstrate the hydrothermal synthesis of long copper nanowires based on a simple protocol. We show that the purification of the nanowires is very important and can be achieved easily by wet treatment with glacial acetic acid. Fabrication of random networks of purified copper nanowires leads to flexible transparent electrodes with excellent optoelectronic performances （e.g., 55 Ω/sq. at 94% transparency）. The process is carried out at room temperature and no post-treatment is necessary. Hybrid materials with the conductive polymer PEDOT：PSS show similar properties （e.g., 46 Ω/sq, at 93% transparency）, with improved mechanical properties. Both electrodes were integrated in capacitive touch sensors.
文摘A new method of enantioselective synthesis of （S,S）-2,8-diazobicyclo [4.3.0] nonane was found by using （R）-2-amino-2- phenyl-ethanol as chiral induction reagent. The entire synthetic process included 8 steps which were easy to operate with high yield. The purification method was only simple recrystallization or even used directly in the next step without further purifica- tion. The total yield was 29%.
