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Synthesis of Isonucleoside-Incorporated Oligonucleotides and Their Binding Abilities with Complementary Sequences
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作者 史继峰 王占黎 +1 位作者 张亮仁 张礼和 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第1期4-9,共6页
Aim To synthesize isonucleoside-incorporated oligonucleotides and investigatetheir binding abilities with complementary sequences. Methods The synthesis was performed on DNAsynthesizer, and the binding behavior was in... Aim To synthesize isonucleoside-incorporated oligonucleotides and investigatetheir binding abilities with complementary sequences. Methods The synthesis was performed on DNAsynthesizer, and the binding behavior was investigated by thermal denaturation studies. Results Fourkinds of single isonucleoside containing oligonucleotides were synthesized. The results of thermaldenaturation showed that the existence of isonucleoside decreased the stability of duplex, and theeffect was more obvious when the isonucleoside was in the middle of the sequence. No obviousdifference was observed when 6'-OH of isonucleoside was free or was protected by allyl group.Conclusions The existence of isonucleoside in oli-gonucleotide makes chain twist and decreased thestability of duplex. 展开更多
关键词 isonucleoside NUCLEOTIDES HYBRIDIZATION thermal denaturation
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合成生物学研究的进展 被引量:15
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作者 张春霆 《中国科学基金》 CSCD 北大核心 2009年第2期65-69,共5页
本文简要介绍了合成生物学发展的历史背景与定义,它的主要研究内容,包括基因线路、合成基因组、合成药物与生物基产品或材料等。探讨了合成生物学与基因工程的异同,介绍了合成生物学在中国的发展情况,讨论了伦理道德与安全问题,最后展... 本文简要介绍了合成生物学发展的历史背景与定义,它的主要研究内容,包括基因线路、合成基因组、合成药物与生物基产品或材料等。探讨了合成生物学与基因工程的异同,介绍了合成生物学在中国的发展情况,讨论了伦理道德与安全问题,最后展望了合成生物学的发展前景。 展开更多
关键词 合成生物学 基因线路 合成基因组 合成药物 合成生物基产品或材料 合成dna序列
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Assembly of long DNA sequences using a new synthetic Escherichia coli-yeast shuttle vector 被引量:3
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作者 Zheng Hou Zheng Zhou +1 位作者 Zonglin Wang Gengfu Xiao 《Virologica Sinica》 SCIE CAS CSCD 2016年第2期160-167,共8页
Synthetic biology is a newly developed field of research focused on designing and rebuilding novel biomolecular components, circuits, and networks. Synthetic biology can also help understand biological principles and ... Synthetic biology is a newly developed field of research focused on designing and rebuilding novel biomolecular components, circuits, and networks. Synthetic biology can also help understand biological principles and engineer complex artificial metabolic systems. DNA manipulation on a large genome-wide scale is an inevitable challenge, but a necessary tool for synthetic biology. To improve the methods used for the synthesis of long DNA fragments, here we constructed a novel shuttle vector named p GF(plasmid Genome Fast) for DNA assembly in vivo. The BAC plasmid p CC1 BAC, which can accommodate large DNA molecules, was chosen as the backbone. The sequence of the yeast artificial chromosome(YAC) regulatory element CEN6-ARS4 was synthesized and inserted into the plasmid to enable it to replicate in yeast. The selection sequence HIS3, obtained by polymerase chain reaction(PCR) from the plasmid p BS313, was inserted for screening. This new synthetic shuttle vector can mediate the transformation-associated recombination(TAR) assembly of large DNA fragments in yeast, and the assembled products can be transformed into Escherichia coli for further amplification. We also conducted in vivo DNA assembly using p GF and yeast homologous recombination and constructed a 31-kb long DNA sequence from the cyanophage PP genome. Our findings show that this novel shuttle vector would be a useful tool for efficient genome-scale DNA reconstruction. 展开更多
关键词 yeast plasmid shuttle dna recombination Genome assembly inserted amplification homologous
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