期刊文献+
共找到250篇文章
< 1 2 13 >
每页显示 20 50 100
关于段玉裁闭口韵阳入合部的押韵和谐声理据的探讨 被引量:2
1
作者 杜恒联 《江南大学学报(人文社会科学版)》 2010年第5期110-114,共5页
段玉裁创立了古韵十七部。他的第七部是侵缉合部,第八部是谈盍合部,即第七第八两部闭口韵是阳入合部。但在他的《诗经韵分十七部表》和《群经韵分十七部表》中并没有阳入押韵的例子。这显然违反了从押韵事实出发归纳韵部的原则。但在谐... 段玉裁创立了古韵十七部。他的第七部是侵缉合部,第八部是谈盍合部,即第七第八两部闭口韵是阳入合部。但在他的《诗经韵分十七部表》和《群经韵分十七部表》中并没有阳入押韵的例子。这显然违反了从押韵事实出发归纳韵部的原则。但在谐声字里,却有大量的侵缉相谐和谈盍相谐现象。这种押韵和谐声的不平衡现象,值得深入探讨。 展开更多
关键词 古韵 押韵 谐声 侵缉合部 谈盍合部
下载PDF
早古生代北秦岭—北祁连结合部构造格局的地层及构造岩浆事件约束 被引量:74
2
作者 徐学义 何世平 +3 位作者 王洪亮 张二朋 陈隽璐 孙吉明 《西北地质》 CAS CSCD 2008年第1期1-21,共21页
通过对位于华北、北秦岭、北祁连交界部位的武威—贺兰山—礼泉地区奥陶纪地层格架进行对比分析,初步认为奥陶纪时,北祁连洋、北秦岭洋和华北西南海盆相通。早—中奥陶世,自鄂尔多斯碳酸盐台地—鄂尔多斯西南缘—北祁连走廊台地边缘—... 通过对位于华北、北秦岭、北祁连交界部位的武威—贺兰山—礼泉地区奥陶纪地层格架进行对比分析,初步认为奥陶纪时,北祁连洋、北秦岭洋和华北西南海盆相通。早—中奥陶世,自鄂尔多斯碳酸盐台地—鄂尔多斯西南缘—北祁连走廊台地边缘—北祁连火山沉积盆地,存在一个基本连续,由陆缘相至盆地相的沉积格架。对构造岩浆事件的对比研究表明,北秦岭西段的关子镇蛇绿岩中的火山岩具N-MORB特点,武山高家河蛇绿岩中火山岩具E-MORB特征,它们均形成于洋中脊环境,是早古生代商丹洋盆西延的证据。对北祁连东端的早古生代地质体初步确认有:具有N-MORB特征的赤沙火山岩系;形成于弧后环境的红土堡基性火山岩系和形成于岛弧环境的陈家河酸性火山岩系,显示了北祁连东端保存有早古生代洋盆及俯冲构造演化的地质记录。岩石学、地球化学及同位素年代学数据揭示北秦岭西端和北祁连东端均发育450~391Ma俯冲碰撞型花岗质岩浆侵入事件及408~415Ma碰撞变质事件,表明北秦岭和北祁连经历了相似的洋陆转化过程。因此,北秦岭和北祁连造山带在新元古代末—早古生代可能为同一洋盆演化的产物,经历了大体相当的构造-热事件的改造,两者可能在统一的构造动力学机制下经历了相似的构造演化,形成了大体相当的古构造格局。对比研究认为,东秦岭商丹断裂带向西可能与武山-唐藏断裂带相对应;北秦岭的二郎坪群向西可能与北祁连的葫芦河群相对应;北秦岭的秦岭群可能与北祁连的陇山群相对应。 展开更多
关键词 构造格局 地层格架对比 构造岩浆事件约束 早古生代 北秦岭-北祁连给合部
下载PDF
基于学前教育专业特点的声乐课“五部合一教学法” 被引量:4
3
作者 葛剑 葛泽扬 《高教学刊》 2018年第21期104-106,共3页
"五部合一教学法"针对学前教育专业的具体培养目标进行声乐教学,从学生的"手、眼、声、情、表"五个方面进行整体联动的综合式训练,避免了声乐课只解决声音问题的不足,在"以眼为帅,以手为从,以情为纲,以声为用... "五部合一教学法"针对学前教育专业的具体培养目标进行声乐教学,从学生的"手、眼、声、情、表"五个方面进行整体联动的综合式训练,避免了声乐课只解决声音问题的不足,在"以眼为帅,以手为从,以情为纲,以声为用,以表为综"的原则指导下,逐步达到"五部合一、联动而歌"在学前教育专业的声乐教学中运用"五部合一教学法"进行教学活动,教学效果显著。 展开更多
关键词 学前教育 声乐教学 -教学法
下载PDF
颈部食管胃吻合口瘘的防治及护理体会
4
作者 王文雅 牟凤萍 王晓娟 《中国医药指南(学术版)》 2008年第19期177-178,共2页
颈部食管胃吻合术是治疗食管癌的常用手术方法之一,其吻合口瘘的发生率相对较高,2002年1月至2007年5月我科实施胃代食管颈部食管吻合术治疗食管肿瘤116例,发生吻合口瘘6例,发生瘘为5.1%,现报道如下:
关键词 食管胃 护理体会
下载PDF
机器人辅助腹腔镜联合膀胱软镜治疗肾盂输尿管连接部梗阻合并肾结石 被引量:3
5
作者 李学超 杨涛 +6 位作者 凡航 孔令生 董金凯 王惠先 刘海燕 李学松 陈立军 《现代泌尿外科杂志》 CAS 2020年第10期902-905,922,共5页
目的评价机器人辅助腹腔镜联合膀胱软镜治疗肾盂输尿管连接部狭窄(UPJO)合并肾结石的临床疗效。方法回顾性分析2019年9月至12月在中国人民解放军总医院行机器人辅助腹腔镜联合膀胱软镜治疗肾盂输尿管连接部梗阻合并肾结石6例患者的临床... 目的评价机器人辅助腹腔镜联合膀胱软镜治疗肾盂输尿管连接部狭窄(UPJO)合并肾结石的临床疗效。方法回顾性分析2019年9月至12月在中国人民解放军总医院行机器人辅助腹腔镜联合膀胱软镜治疗肾盂输尿管连接部梗阻合并肾结石6例患者的临床资料。统计手术时长、出血量、术后住院时间、清石率、手术成功率及并发症发生情况。结果6例患者手术均获成功,无中转开放及改为二期手术,总手术时间87~136 min,平均(98.17±12.61)min,膀胱软镜取石时间56~85 min,平均(30.76±8.33)min,术中失血量20~100 mL,平均(45.83±20.83)mL,引流管留置时间3~7 d,平均(4.5±1.5)d,术后住院时间4~8 d,平均(6.33±1.22)d,术后随访2~5个月,中位随访时间3.5个月,所有患者肾盂积液较术前明显减轻,血肌酐正常,未见结石残留及复发。结论机器人辅助腹腔镜联合膀胱软镜治疗肾盂输尿管连接部狭窄(UPJO)合并肾结石可同期进行且安全、有效。 展开更多
关键词 肾盂输尿管连接梗阻 肾结石 机器人辅助腹腔镜 膀胱软镜
下载PDF
从“番部合乐”窥清燕飨乐队成因
6
作者 张珂 《歌海》 2018年第4期43-49,共7页
《皇朝礼器图式》作为清代礼书之最,在研究清史中具有十分重要的地位。书中第九卷对"燕飨番部合乐乐队"之乐器具有详细描述,是研究清乾隆时期燕飨音乐的重要资料。开篇通过对《皇朝礼器图式》中"燕飨番部合乐"之乐... 《皇朝礼器图式》作为清代礼书之最,在研究清史中具有十分重要的地位。书中第九卷对"燕飨番部合乐乐队"之乐器具有详细描述,是研究清乾隆时期燕飨音乐的重要资料。开篇通过对《皇朝礼器图式》中"燕飨番部合乐"之乐队、乐器进行分析,再结合"新史学"的部分研究思维,从统治者"大一统"的思想角度出发,深入探究清王朝统治者对满、汉及其他少数民族音乐的态度及成因。 展开更多
关键词 燕飨乐队 《皇朝礼器图式》 “新清史”
下载PDF
PMCV方案术前诱导化疗治疗口腔颌面部鳞癌的近期疗效观察
7
作者 金友仁 马光生 +5 位作者 吴俊培 谢佑恒 翁应时 杨家平 陈君勤 沈言备 《口腔医学纵横》 CSCD 1991年第4期225-227,共3页
作者用 PMCV 方案,术前诱导化疗,平阳霉素0.3mg/kg,静注,第1、5天;长春新硷1.4mg/M^2,静注,第1天;氨甲喋吟25mg/M^2,静注,第1天;环磷酰胺600mg/M^2,静注,第2天.连用三周,停药一周,可重复疗程.共治疗口腔颌面部鳞癌46例,总有效率76.1%.... 作者用 PMCV 方案,术前诱导化疗,平阳霉素0.3mg/kg,静注,第1、5天;长春新硷1.4mg/M^2,静注,第1天;氨甲喋吟25mg/M^2,静注,第1天;环磷酰胺600mg/M^2,静注,第2天.连用三周,停药一周,可重复疗程.共治疗口腔颌面部鳞癌46例,总有效率76.1%.初治者37例,有效率78.3%,复治者9例,有效率66.7%,该方案主要副毒反应有消化道不适、白细胞下降、脱发、发热等,但多不严重,可以耐受。 展开更多
关键词 PMCV方案 口腔鳞癌 术前诱导化疗 鳞癌 药物疗法 治疗
下载PDF
A Hypothesis Regarding the Origin of Additional Surface Acidity in Solid Complexes with Same Metal Cations
8
作者 SHAO Bo TONG Chaoli +5 位作者 WANG Jiaqian HAN Zhongkang ZHANG Yan GE Wenfeng WANG Yong YANG Hangsheng 《材料科学与工程学报》 CAS CSCD 北大核心 2024年第5期718-722,共5页
Based on the criteria for additional surface acidity generation in composite oxides and composite fluorides proposed by Tanabe and Kemnitz et al.A hypothesis for the origin of additional surface acidity in solid compo... Based on the criteria for additional surface acidity generation in composite oxides and composite fluorides proposed by Tanabe and Kemnitz et al.A hypothesis for the origin of additional surface acidity in solid composites with the same metal cations is proposed.The surface acidsites of We analyze three types of solid composite systems,that is,CrF_(3)/Cr_(2)O_(3),MgF_(2)/MgO,and ZnF_(2)/ZnO,is systematically analyzed,which agrees with experimental results.Accordingly,the origin of additional surface acidity in these solid composites is reasonably explained,and the types of acidic sites are also predicted. 展开更多
关键词 Surface acidity Solid composite Local charge imbalance
下载PDF
Compressive performance of innovative reinforced pillars in closed/abandoned mines
9
作者 LI Jian BAI Jin-wen +6 位作者 FENG Guo-rui WANG Shan-yong ZHAO Hong-chao MI Jia-chen PAN Rui-kai SHI Xu-dong MA Jun-biao 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第8期2780-2793,共14页
Pillar is closely related to the stability and reliability of underground spaces in closed/abandoned mines.The present research introduced a new technique to strengthen square cement mortar columns via fiber-reinforce... Pillar is closely related to the stability and reliability of underground spaces in closed/abandoned mines.The present research introduced a new technique to strengthen square cement mortar columns via fiber-reinforced polymer(FRP)strips to verify the strengthening effect of FRP on pillars.Compared to a fully wrapped FRP jacket,the advantages of FRP strip are cost-effective and easy-to-construct.A series of compression tests as well as theoretical analysis were carried out to explore the mechanical behavior of square cement mortar specimens partially strengthened with FRP strips.The results verified the effectiveness of FRP strips in enhancing the stress and strain of cement mortar.Different from unconfined cement mortar specimens,these FRP-strengthened cement mortar specimens are featured with the double-peaked behaviors,mainly attributed to the stress state transformation from a one-dimensional to a three-dimensional stress state.It also indicated that the enhancement of stress increased with the FRP strip width.Moreover,the brittle-ductile transition ductile failure characteristics were also observed in FRP-confined cement mortar specimens.The ultimate ductility of the cement mortar specimen decreases gradually with the growth of the FRP strip width.The main contribution of this research is to enrich the strengthening techniques for residual pillars. 展开更多
关键词 fiber-reinforced polymer uniaxial compression partially strengthening double-peaked behaviors brittle-ductile transition
下载PDF
我国蚌科一新纪录
10
作者 刘月英 张文珍 +1 位作者 王耀先 段毅豪 《动物分类学报》 CSCD 1992年第2期150-150,共1页
查氏拟齿蚌Pseudodon chaperi (Morgan, 1885) 见壳近似长方形,略膨胀。前部短圆,后背缘呈截状与后缘相交处有一不明显的角。壳顶部低平,略低于后背缘,位于贝壳的前部,距壳前端1/3处。腹缘略直。壳面呈黄褐色或棕褐色,常被腐蚀。
关键词 一新纪录 中国科学院 动物研究所 后背缘 蚌科 云南西双版纳 壳顶腔 拟主齿 望天树 合部
全文增补中
释绍昙诗韵研究 被引量:3
11
作者 丁治民 舟人 《古汉语研究》 CSSCI 北大核心 2004年第3期19-23,共5页
释绍昙是南宋末密庵咸杰系名僧 ,其籍贯为西蜀 ,自进住径山寺之后直至去世之前的生活区域主要是在两浙东、西路之间 ;其诗韵颇有特点 ,许多诗就是以方音入韵 ,但其韵系所反映的并不是这两地的语音 ,而是闽语 。
关键词 释绍昙 南宋 诗韵 方音 闽语
下载PDF
Fracture behavior of double-pass TIG welded 2219-T8 aluminum alloy joints under transverse tensile test 被引量:5
12
作者 李权 吴爱萍 +3 位作者 赵玥 王国庆 鄢东洋 吴会强 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第6期1794-1803,共10页
2219-T8 aluminum alloys were butt welded by the double-pass tungsten inert gas (TIG) arc welding process. The transverse tensile test of the joint showed that the fracture mainly occurred in the partially melted zo... 2219-T8 aluminum alloys were butt welded by the double-pass tungsten inert gas (TIG) arc welding process. The transverse tensile test of the joint showed that the fracture mainly occurred in the partially melted zone (PMZ). Effects of the PMZ on the fracture behavior were systematically studied. Continuous intergranular eutectics were observed in the PMZ close to the fusion line. Away from the fusion line, the intergranular eutectics in the PMZ became discontinuous. The fracture morphology and the microhardness distribution of the joint showed that the PMZ was gradient material with different mechanical properties, which strongly affected the fracture process. It was observed that the crack initiated in the PMZ near the front weld toe, and propagated in the PMZ away from the fusion line. Then, the crack tip was blunt when it propagated into the PMZ with higher plasticity. Finally, the rest part of the joint was shear fractured. 展开更多
关键词 2219 aluminum alloy tungsten inert gas arc welding partially melted zone fracture behavior
下载PDF
Microstructural evolution and phase transformation during partial remelting of in-situ Mg_2Si_p/AM60B composite
13
作者 张素卿 陈体军 +1 位作者 程发良 李雷亮 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2016年第6期1564-1573,共10页
The microstructural evolution and phase transformations during partial remelting of in-situ Mg2Sip/AM60B composite modified by SiC and Sr were investigated. The results indicate that SiC and Sr are effective for refi... The microstructural evolution and phase transformations during partial remelting of in-situ Mg2Sip/AM60B composite modified by SiC and Sr were investigated. The results indicate that SiC and Sr are effective for refining primary α-Mg grains and Mg2Si particles. After being partially remelted, a semisolid microstructure with small and spheroidal primary α-Mg particles can be obtained. The microstructural evolution during partial remelting can be divided into four stages: the initial rapid coarsening, structural separation, spheroidization and final coarsening, which are essentially caused by the phase transformations of β→α, α+β→L and α→L, α→L, and α→L and L→α, respectively. The Mg2Si particles have not obvious effect on the general microstructural evolution steps, but can slower the evolution progress and change the coarsening mechanism. During partial remelting, Mg2Si particles first become blunt and then become spheroidal because of melting of their edges and corners, and finally are coarsened owing to Ostwald ripening. 展开更多
关键词 magnesium alloy partial remelting THIXOFORMING microstructure evolution phase transformation in-situ composite
下载PDF
Feature fusing in face recognition 被引量:1
14
作者 于威威 滕晓龙 刘重庆 《Journal of Southeast University(English Edition)》 EI CAS 2005年第4期427-431,共5页
With the aim of extracting the features of face images in face recognition, a new method of face recognition by fusing global features and local features is presented. The global features are extracted using principal... With the aim of extracting the features of face images in face recognition, a new method of face recognition by fusing global features and local features is presented. The global features are extracted using principal component analysis (PCA). Active appearance model (AAM) locates 58 facial fiducial points, from which 17 points are characterized as local features using the Gabor wavelet transform (GWT). Normalized global match degree (local match degree) can be obtained by global features (local features) of the probe image and each gallery image. After the fusion of normalized global match degree and normalized local match degree, the recognition result is the class that included the gallery image corresponding to the largest fused match degree. The method is evaluated by the recognition rates over two face image databases (AR and SJTU-IPPR). The experimental results show that the method outperforms PCA and elastic bunch graph matching (EBGM). Moreover, it is effective and robust to expression, illumination and pose variation in some degree. 展开更多
关键词 face recognition feature fusion global features local features
下载PDF
Combining Ability and Correlation of Ear Characteristics of Twenty Maize Inbred Lines
15
作者 李淑君 董昕 +3 位作者 付忠军 祁志云 张丕辉 周汝平 《Agricultural Science & Technology》 CAS 2017年第9期1601-1606,共6页
The combining ability and correlation of eight ear characteristics in 99 maize hybrids generated by crossing nine female parents with 11 male parents were analyzed by incomplete diallel cross (NC II ) design. The re... The combining ability and correlation of eight ear characteristics in 99 maize hybrids generated by crossing nine female parents with 11 male parents were analyzed by incomplete diallel cross (NC II ) design. The results showed that the line F6 had the highest general combining ability (GCA) for yield, followed by F7, M3, M4 and M8. All of the five lines have great potential in maize breeding. The cross combination M3xF10 had the highest specific combining ability (SCA) for yield, showing strong heterosis. Heritability analysis of ear characteristics showed that GCA variance was higher than SCA variance in ear diameter, number of rows per ear and seed rate, and they were mainly controlled by the additive gene effect, indicating that that the selections for these traits are effective at early generations. The other three traits had lower SCA, for which the selections should be carried out at late generations. The correlation analysis revealed that ear length, number of grains per row, ear diameter, number of rows per ear, 100-seed weight and seed rate had extremely significant positive correlations with grain yield per plant. Among them, number of grains per row had the most significant effect on yield per plant. Barren tip length had a significant negative correlation with grain yield per plant. Therefore, we concluded that the combinations with more grains per row and shorter barren tip should be selected to achieve high yield of maize. 展开更多
关键词 MAIZE Inbred line Combining ability Ear characteristics Genetic parameters
下载PDF
笔记二则 被引量:1
16
作者 布谷 《赤峰学院学报(哲学社会科学版)》 1995年第1期1-2,8,共3页
八尺以上的高头大马谓之龙;或作,从戎得声,而戎有大义.隆确丰大义,而龙隆同属来母号,又属东冬合部.龙者,大也.龙,宠也;宠,荣也.在红山文化中,酋长们以佩带玉龙为荣.龙和宠是同源词,其远古声母盖为复辅音·[t‘-]... 八尺以上的高头大马谓之龙;或作,从戎得声,而戎有大义.隆确丰大义,而龙隆同属来母号,又属东冬合部.龙者,大也.龙,宠也;宠,荣也.在红山文化中,酋长们以佩带玉龙为荣.龙和宠是同源词,其远古声母盖为复辅音·[t‘-].总之。龙的原始义是伟大而光荣也.DHA(二十二碳六烯酸)是人类脑细胞发育即智能发展的非常的必要条件.DHA只富含中鱼类中,而陆上动植物却极少含有.所以说,在旧石器—新石器—青铜器的历史演进中,史前渔业的意义,要比采集业、狩猎业、农业的意义重要百倍. 展开更多
关键词 上古史 语源 东冬合部 复辅音·[t'Ⅰ-]·[Kl-] 红山文化玉猪龙 史前渔业 DHA 脑细胞突起 新石器 玉器 青铜器 海洋文化
下载PDF
Combining Ability Analysis of Panicle Traits in Six CIMMYT Maize Inbred Lines
17
作者 付忠军 李淑君 +2 位作者 董昕 祁志云 张丕辉 《Agricultural Science & Technology》 CAS 2015年第12期2653-2655,2663,共4页
In this study, six CIMMYT maize inbred lines and five representative do- mestic maize inbred lines were used as parental lines. By using incomplete diallel cross design, 30 hybrid combinations were developed to analyz... In this study, six CIMMYT maize inbred lines and five representative do- mestic maize inbred lines were used as parental lines. By using incomplete diallel cross design, 30 hybrid combinations were developed to analyze the general com- bining ability (GCA), specific combining ability (SCA) and total combining ability (TCA) of seven panicle traits in six CIMMYT maize inbred lines. The results showed that CIMBL98 and GEMS13 were excellent inbred lines with good compre- hensive performance; CIMBL98 × 340 and GEMS13×Chang 7-2 were superior combinations. 展开更多
关键词 Maize (Zea mays L.) Inbred line Panicle traits Combining ability
下载PDF
ALA Altered ABA Content of Winter Oilseed Rape(Brassica napus L.)Seedling 被引量:1
18
作者 张树杰 李玲 +1 位作者 张春雷 李光明 《Agricultural Science & Technology》 CAS 2011年第4期484-488,591,共6页
[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA... [Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA)contents in plant organs and xylem saps were investigated through hydroponic experiment.Four treatments including seed soaking with 0,25,50 and 75 mg/L ALA solutions(CK,A1,A2 and A3)for 6 h were set in the test.[Result] In addition to A3 treatment which made the dry weights of oilseed rape seedlings slightly decrease,after seed soaking with ALA,dry weights,net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of plants significantly increased compared with control.ABA concentrations in plant shoots and xylem saps increased in different levels after seed soaking with ALA.[Conclusion] The increasing endogenous ABA contents might be an explanation for promotion effect of ALA application on the growth of winter oilseed rape seedling. 展开更多
关键词 Oilseed rape Hydroponic culture Xylem sap PHOTOSYNTHESIS PHYTOHORMONE
下载PDF
三角帆蚌
19
作者 杨品红 《农村百事通》 2015年第16期41-41,73,共2页
三角帆蚌俗称翼蚌、劈蚌、珍珠蚌、三角蚌,是一种淡水双壳类软体动物,属瓣鳃纲、蚌科、帆蚌属。三角帆蚌广泛分布于湖南、湖北、安徽、江苏、浙江、江西等地,尤以洞庭湖及中型湖泊分布较多。1.形态特征。三角帆蚌贝壳大而侧扁,具有向上... 三角帆蚌俗称翼蚌、劈蚌、珍珠蚌、三角蚌,是一种淡水双壳类软体动物,属瓣鳃纲、蚌科、帆蚌属。三角帆蚌广泛分布于湖南、湖北、安徽、江苏、浙江、江西等地,尤以洞庭湖及中型湖泊分布较多。1.形态特征。三角帆蚌贝壳大而侧扁,具有向上突起的三角帆状后翼,外形略呈三角形。贝壳最长可达240毫米,壳面呈黄褐色,壳顶外缘有数条由结节突起组成的斜行粗肋,铰合部发达,左右壳各有2枚拟主齿,左壳有2枚侧齿, 展开更多
关键词 三角帆蚌 帆蚌属 蚌科 合部 左壳 珍珠蚌 壳顶 形态特征 双壳类 钩介幼虫
下载PDF
Interactions Between Anticancer Pt(II) complexes and Human Erythrocyte Spectrin *
20
作者 杨晓改 李荣昌 《Journal of Chinese Pharmaceutical Sciences》 CAS 1998年第3期9-14,共6页
The interactions between human erythrocyte spectrin(SP) and Pt(II) complexes with different composition and configuration were studied by fluorescence and circular dichroism spectra. The results showed that there are ... The interactions between human erythrocyte spectrin(SP) and Pt(II) complexes with different composition and configuration were studied by fluorescence and circular dichroism spectra. The results showed that there are 4.7×10 2 binding sites of cisplatin(CDDP) in a spectrin tetramer(SPT). Among them, about 70 sites with apparent binding constant K 1】3.47×10 6 were of highest affinity, 1.8×10 2 sites with K 2 = 3.47×10 6 were of high affinity, and other 2.2×10 2 sites with K 3 = 8.77×10 5 were of low affinity. The conformation change of spectrin, depending on the concentration of Pt(II) complex and molar ratio(R) of Pt(II) complex to spectrin, was induced by the binding of Pt(II) complexes. It indicated that the interaction of both CDDP and cis diaquodiamine platinum(DADP) with SP followed a two step first order kinetic process in the first stage (1 h), and the kinetic constants were determined. In the second stage, the induced conformation change, polymerization and depolymerization of SP were probably involved. It was noticed that in the reaction of SP and Pt(II) complexes with 1,2 cyclohexanediammine isomers as chiral carrier ligand, stereo matching played a more important role than the affinity of Pt(II) to thiol groups of SP. 展开更多
关键词 Pt (II) complex SPECTRIN CONFORMATION Binding sites Kinetic CHIRAL
全文增补中
上一页 1 2 13 下一页 到第
使用帮助 返回顶部