论西南少数民族水文化的主要特征

纵观目前少数民族水文化的研究现状,大多数成果集中在对单一民族水文化的内涵与功能的静态描述与分析,鲜有对作为一个水生态共同体的少数民族水文化整体运行的独特性与内在规律的动态解析。西南少数民族水文化是中华水文化的重要组成部分,它具有中华水文化一般的特点,也具有鲜明的个性特征。西南少数民族水文化主要有神圣性、全民性、整体性、生活化、生态性与局限性六大特征。西南少数民族水文化的任何一个特征,事实上在日常的水事活动中,均不可能单独存在和运行,它们共同构成一个联动的整体,在西南少数民族水文化实践中,共时存在,同时表征。

Analysis of Gene Expression of Seven Isoforms of ADP-glucose Pyrophosphorylase in Rice Endosperm under Different Temperature Conditions

[Objective] This study aimed to analyze the effects of temperature on the expression of AGPase isoform genes in rice endosperm during milk stage. [Method] Different temperature treatments （33 and 25 ℃ of daily mean temperature for high and normal temperature treatments, respectively） and the real-time fluorescence quantitative PCR （ FQPCR） were used to analyze the expression patterns of seven isoforms （AGPS1, AGPS2a, AGPS2b, AGPL1, AGPL2, AGPL3 and AGPL4） of ADPglucose pyrophosphorylase （AGPase） which was the key enzyme in starch synthesis and metabolism in rice endosperm of two rice varieties Teqing and Thai Fragrant Rice. [Result] The AGPase isoforms AGPS2b, AGPL2 and AGPL3 had much higher expression than the other four isoforms, thus they were thought to be the main expression patterns of AGPase in rice endosperm. The relative expressions of AGPL2 was the highest among all the isoforms. The relative expressions of AGPS2b, AGPL2 and AGPL3 were higher in the normal temperature treatment than in the high temperature treatment in both rice varieties. The relative expression of the three enzyme genes in milk stages in Teqing was higher than those in Thai Fragrant Rice under different temperature treatments. [Conclusion] This study provides a theoretical basis for further use of molecular biology techniques to cultivate stable high-quality rice varieties.