To investigate the tissue-specificities of isozymes and the genetic structureof wild spotted halibut ( Verasper variegatus) population, horizontal starch gel electrophoresiswas performed on 45 individuals collected in...To investigate the tissue-specificities of isozymes and the genetic structureof wild spotted halibut ( Verasper variegatus) population, horizontal starch gel electrophoresiswas performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymesin 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The resultsshowed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes(AAT, ADH, EST, OPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues(eye, skeleton muscle, liver and heart) were selected for genetic analysis. Fourteen isozymes areencoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1~*, GPI-2~*,G3PDH~*, IDHP-1~*, LDH~*, MPI~*, PGM-1~*. PGM-2~* and SDH~*, and the proportion of polymorphic lociis 0.4500 (P_(0.99)) ? The mean values of observed and expected heterozygosities are 0.0278and 0.0265, respectively and the average effective number of alleles is 1.0675.展开更多
Spray structure and atomization characteristics were investigated through a comparison of a porous and a shear coaxial injector. The porous injector shows better atomization performance than the shear coaxial injector...Spray structure and atomization characteristics were investigated through a comparison of a porous and a shear coaxial injector. The porous injector shows better atomization performance than the shear coaxial injector. To in- crease atomization performance and mixing efficiency of two-phase jets, a coaxial porous injector which can be applicable to liquid rocket combustors was designed and tested. The characteristics of atomization and spray from a porous and a shear coaxial injector were characterized by the momentum flux ratio. The breakup mechanism of the porous injector is governed by Taylor-Culick flow and axial shear forces. Momentum of injected gas flow through a porous material which is composed of sintered metal is radically transferred to the center of the liquid column, and then liquid column is effectively broken up. Although the shapes of spray from porous and shear co- axial jets were similar for various momentum ratio, spray structures such as spray angle and droplet sizes were different. As increasing the momentum flux ratio, SMD from the porous injector showed smaller value than the shear coaxial injector展开更多
文摘To investigate the tissue-specificities of isozymes and the genetic structureof wild spotted halibut ( Verasper variegatus) population, horizontal starch gel electrophoresiswas performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymesin 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The resultsshowed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes(AAT, ADH, EST, OPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues(eye, skeleton muscle, liver and heart) were selected for genetic analysis. Fourteen isozymes areencoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1~*, GPI-2~*,G3PDH~*, IDHP-1~*, LDH~*, MPI~*, PGM-1~*. PGM-2~* and SDH~*, and the proportion of polymorphic lociis 0.4500 (P_(0.99)) ? The mean values of observed and expected heterozygosities are 0.0278and 0.0265, respectively and the average effective number of alleles is 1.0675.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korean Government(MEST)(NRF-2011-0015435 and NRF-2012M 1A3A3A02033146)
文摘Spray structure and atomization characteristics were investigated through a comparison of a porous and a shear coaxial injector. The porous injector shows better atomization performance than the shear coaxial injector. To in- crease atomization performance and mixing efficiency of two-phase jets, a coaxial porous injector which can be applicable to liquid rocket combustors was designed and tested. The characteristics of atomization and spray from a porous and a shear coaxial injector were characterized by the momentum flux ratio. The breakup mechanism of the porous injector is governed by Taylor-Culick flow and axial shear forces. Momentum of injected gas flow through a porous material which is composed of sintered metal is radically transferred to the center of the liquid column, and then liquid column is effectively broken up. Although the shapes of spray from porous and shear co- axial jets were similar for various momentum ratio, spray structures such as spray angle and droplet sizes were different. As increasing the momentum flux ratio, SMD from the porous injector showed smaller value than the shear coaxial injector