A MADS box gene （GhMADS3） was cloned from cotton （Gossypium hirsutum L.） based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum m...A MADS box gene （GhMADS3） was cloned from cotton （Gossypium hirsutum L.） based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum majus FAR, and Arabidopsis thaliana AG, respectively, and was grouped with AG homologues when the full length sequences excluding N-extensions were compared. GhMADS3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to AG in Arabidopsis. However, it was not expressed in floral buds of a homeotic cotton variant chvl. Ectopic expression of GhMADS3 in tobacco （Nicotiana tabacum L.） resulted in flowers with sepal-to-carpel and petal-to-stamen transformation. The carpelloid first whorl organs, with stigmatic tissue on their upper edges, had a white appearance when compared with the dark green color of the wild type sepals. At times, long filaments were observed at the fusion site of the first carpelloid oranges. The second whorl organs in staminoid were usually smaller than the wild type and the color was changed from pink to white. These results suggest that GhMADS3 has a homeotic role in flower development.展开更多
文摘目的:通过重组cDNA文库的血清学筛选(SEREX)技术获得来自非洲爪蟾(Xenopus laevis)肝脏肿瘤文库中具有潜在治疗价值的异种同源抗原的cDNA序列。方法:用人肝癌细胞株SMMC-7721免疫家兔后收集的抗血清作为筛选血清,采用SEREX技术筛选Xenopuslaevis肝脏肿瘤cDNA表达文库。结果:通过两轮筛选获得19个阳性克隆,通过DNA序列测定和分子生物学技术确定这19个克隆编码16个蛋白。其中Transgelin 2、RASguanylreleasing protein1和Chromobox protein homolog6可能与肝细胞肝癌有关。结论:用异种免疫血清筛选异种同源肿瘤抗原拓宽了SEREX技术的应用范围,鉴定Transgelin2、RASguanyl releasing protein1和Chromobox protein homolog6可能为异种同源抗原。
基金This work was supported by the National Natural Science Foundation of China(No.30070485)Southwest University InitialResearch Foundation Grant to Doctor(No.D200404).
文摘A MADS box gene （GhMADS3） was cloned from cotton （Gossypium hirsutum L.） based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum majus FAR, and Arabidopsis thaliana AG, respectively, and was grouped with AG homologues when the full length sequences excluding N-extensions were compared. GhMADS3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to AG in Arabidopsis. However, it was not expressed in floral buds of a homeotic cotton variant chvl. Ectopic expression of GhMADS3 in tobacco （Nicotiana tabacum L.） resulted in flowers with sepal-to-carpel and petal-to-stamen transformation. The carpelloid first whorl organs, with stigmatic tissue on their upper edges, had a white appearance when compared with the dark green color of the wild type sepals. At times, long filaments were observed at the fusion site of the first carpelloid oranges. The second whorl organs in staminoid were usually smaller than the wild type and the color was changed from pink to white. These results suggest that GhMADS3 has a homeotic role in flower development.
