不同源水混合对生活饮用水质的影响

[目的 ]探讨地面水和地下水二种水源混水对生活饮用水水质的影响。 [方法 ]对三种混水模式 (地下水的水质状况、与之混合的地面水种类、地面水与地下水混合的比例均有所不同 )连续 3年观察混水前后水中 2 4项卫生学指标的变化。 [结果 ]经混水工艺处理后的水与原地下水相比 ,可以使水中氯化物、硝酸盐、硫酸盐、总硬度和铁的含量降低 30 %～ 70 % ,其中硝酸盐和总硬度的含量减少约 50 % ,并由原地下水水质中硝酸盐和总硬度含量超过生活饮用水卫生标准降至标准限以下。 [结论 ]本文阐述的混水模式应用于生活饮用水中 ,是一种有效改善地下水水质的方法 ,它可以使出厂水的检测指标符合生活饮用水的卫生标准 ,并明显地降低原地下水中无机离子的含量 。

7种药食同源水提物对金黄色葡萄球菌的抑制作用

目的:研究药食同源水提物对金黄色葡萄球菌的抑菌活性。方法:根据《卫生部国家中医药管理局关于印发医疗机构中药煎药室管理规范的通知》对药食同源物质进行水提。用K-B法检验水提物对金黄色葡萄球菌的抑菌效果,得到原始数据,用SPASS 21.0对原始数据进行统计与分析。结果:蒲公英、金银花、甘草、决明子、胖大海、香橼和川贝母水提物浓度为1 g·m L^(-1)时抑菌圈直径最大分别为17.94 mm、19.66 mm、18.59 mm、12.75 mm、11.09 mm、12.44 mm和8.92 mm。结论:金银花对金黄色葡萄球菌抑菌效果最好,甘草次之,川贝母对金黄色葡萄球菌抑菌效果最差。

张仲景“瘀水同源”理论探析

从“瘀水同源”的理论基础、“瘀水同病”的病机、“瘀水同治”的立法与用方等方面分析《金匮要略》对瘀水同源的认识,认为血病可以导致水病,水病也可以导致血病,或者两者相兼为病;治疗上活血可以利水,利水可以活血,并创制了“瘀水同治”的方剂,对临床有重要指导意义。

张仲景“瘀水同源”理论指导腰椎管狭窄症术后治疗临床观察

目的:观察张仲景"瘀水同源"理论在腰椎管狭窄症术后治疗中的作用。方法:对腰椎管狭窄症术后31例患者应用瘀水同治法治疗,随访3月、6月、12月,记录评价日本骨科学会下腰痛(JOA)评分情况。结果:治疗腰椎管狭窄症术后下肢麻痛12月回访优良率达96.7%,治疗后3月、6月、12月患者JOA评分均较治疗前升高(P<0.05)。结论:瘀水同源理论指导治疗腰椎管狭窄症术后下肢麻痛疗效确切。

Modeling Rice rgMT as a Plant Metallothionein-Like Protein by the Distance Geometry and Homology Methods

Rice metallothionein-like protein (rgMT) shows characteristics of a three-section pattern composed of two highly conserved cysteine rich (CR) domains in the terminals and a spacer without cysteine (cys) residues in the center of the molecule. In this paper, the two CR domains and the spacer region were modeled by the distance geometry and homology methods separately. For the CR domains, twenty random models were generated for each cys combination based on the constraint conditions of CXC (C represents cys, X represents any amino acid other than cys), and CXXC motifs and a metal-sulfur chelating cluster. Four models for the N-terminal and two for C-terminal CR domain containing metal chelating structures formed by different combinations of cys were selected from 900 possible conformations. The GOR method was used to predict the secondary structure of the spacer region and its model was built by the homology method. After three parts of the protein were modeled, they were connected to form a three-dimensional structure model of rgMT. The whole conformation showed that rgMT could form two independent metal-sulfur chelating structures connected by a spacer peptide, without a structural or energy barrier for them to form two independent metal-chelating clusters just as mammalian metallothionein (MT) proteins. As all plant metallothionein-like (MT-L) proteins have the same primary structural characteristic, two CR domains connected by a spacer region, and many have the same cys arrangement pattern as rgMT, the three-dimensional structure model of rgMT will provide an important reference for the structural study of other plant MT-L proteins.

Stable Isotope Technique——An Advanced Technology in Ascertaining Plant-Water Relations

Some key terms and relevant standard related with stable isotope tech- nique were introduced, and the determination of carbon, hydrogen and oxygen, the three stable isotopes in water, as well as their isotope effect was discussed. Appli- cations and advances of stable isotopes were stated mainly from plant water source, water use efficiency, the most active water adsorption region in root system and water resources utilization differences among different species in plant communi- ty. In addition, some existing problems in investigating the plant-water relations by using stable isotope technique were put forward. In this study, we aimed to promote the sustainable development of stable isotope technique in the research of plant water utilization and in the field of ecology in China.

Genome-wide Detection and Analysis of Alternative Splicing for Nucleotide Binding Site-Leucine-Rich Repeats Sequences in Rice

Alternative splicing is a major contributor to genomic complexity and proteome diversity, yet the analysis of alternative splicing for the sequence containing nucleotide binding site and leucine-rich repeats （NBS-LRR） domain has not been explored in rice （Oryza sativa L.）. Hidden Markov model （HMM） searches were performed for NBS-LRR domain. 875 NBS-LRR-encoding sequences were obtained from the Institute for Genomic Research （TIGR）. All of them were used to blast Knowledge-based Oryza Molecular Biological Encyclopaedia （KOME）, TIGR rice gene index （TGI）, and Universal Protein Resource （UniProt） to obtain homologous full-length cDNAs （FL-cDNAs）, tentative consensus sequences, and protein sequences. Alternative splicing events were detected from genomic alignment of FL-cDNAs, tentative consensus sequences, and protein sequences, which provide valuable information on splice variants of genes. These sequences were aligned to the corresponding BAC sequences using the Spidey and Sim4 programs and each of the proteins was aligned by tBLASTn. Of the 875 NBS-LRR sequences, 119 （13.6%） sequences had alternative splicing where multiple FL-cDNAs, TGI sequences and proteins corresponded to the same gene. 71 intron retention events, 20 exon skipping events, 16 alternative termination events, 25 alternative initiation events, 12 alternative 5＇ splicing events, and 16 alternative 3＇ splicing events were identified. Most of these alternative splices were supported by two or more transcripts. The data sets are available at http：//www.bioinfor.org. Furthermore, the bioinformatics analysis of splice boundaries showed that exon skipping and intron retention did not exhibit strong consensus. This implies a different regulation mechanism that guides the expression of splice isoforms. This article also presents the analysis of the effects of intron retention on proteins. The C-terminal regions of alternative proteins turned out to be more variable than the N-terminal regions. Finally, tissue distribution and protein localization of alternative splicing were explored. The largest categories of tissue distributions for alternative splicing were shoot and callus. More than one-thirds of protein localization for splice forms was plasma membrane and cytoplasm. All the NBS-LRR proteins for splice forms may have important function in disease resistance and activate downstream signaling pathways.

Application of isotopic and hydro-geochemical methods in identifying sources of mine inrushing water

Isotopic and hydro-geochemical surveys were carried out to identify the source of mine inrushing water at the #73003 face in the Laohutai Mine. Based on the analysis of isotopes and hydro-chemical features of surface water, groundwater from different levels and the inrushing water, a special relationship between water at the #73003 face and cretaceous water has been found. The results show that the isotopic and hydro-chemical features of the inrushing water are completely different from those of other groundwater bodies, except for the cretaceous water. The isotopic and hydrochemical characteristics of cretaceous water are similar to the inrushing water of the #73003 face, which aided with obtaining the evidence for the possible source of the inrushing water at the #73003 face. The isotope calculations show that the inrushing water at the #73003 face is a mixture of cretaceous water and Quaternary water. Water from the cretaceous conglomerate is the main source, accounting for 67% of the inrushing water, while the Quaternary water accounts for 33%. The conclusion is also supported by a study of inrushing-water channels and an active fault near the inrushin^-water plot on the #73003 face.

Observation on Double Fertilization and Early Embryonic Development in Autotetraploid Polyembryonic Rice

The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.

Stable Isotopes in Precipitation in Xilin River Basin,Northern China and Their Implications

Under the increasing pressure of water shortage and steppe degradation, information on the hydrological cycle in steppe region in Inner Mongolia, China is urgently needed. An intensive investigation of the temporal varia-tions of δD and δ^18O in precipitation was conducted in 2007-2008 in the Xilin River Basin, Inner Mongolia in the northern China. The 6D and δ^18O values for 54 precipitation samples range from ＋1.1%o to -34.7%0 and -3.0%0 to -269%0, respectively. This wide range indicates that stable isotopes in precipitation are primarily controlled by differ-ent condensation mechanisms as a function of air temperature and varying sources of vapor. The relationship between δD and δ^18O defined a well constrained line given by δD = 7.896180 ＋ 9.5, which is nearly identical to the Meteoric Water Line in the northern China. The temperature effect is clearly displayed in this area. The results of backward tra-jectory of each precipitation day show that the vapor of the precipitation in cold season （October to March） mainly originates from the west while the moisture source is more complicated in warm season （April to September）. A light precipitation amount effect existes at the precipitation event scale in this area. The vapor source of precipitation with higher d-excesses are mainly from the west wind or neighboring inland area and precipitation with lower d-excesses from a monsoon source from the southeastern China.

The Study of the Effect of Educational Level of Students and English Language Teachers＇ Awareness on True and False Cognates in Iran

The ability to speak two languages in the non-English-speaking countries is a remarkable achievement. There is a good reason to believe that bilingualism is the norm for the majority of people in the world, because 70% of the earth＇s population are supposed to be bilingual or multilingual. Our purpose of the study was to determine the participants＇ awareness of using true and false cognate words of L 1 （the first language） ＆ L2 （the second language） in learning English. Three hundred and eighty-five selected people from 3,789 statistical population participated in 2014--2015 at three levels of A.D., B.A., and M.A. of Islamic Azad University as well as teachers of English in English educational institutions in Zanjan （Iran）. Each educational level was considered as one category with the total sample calculated by using Kokran Formula, and the amount of each category was determined by using appropriate proportion and randomized categorical sampling method. The participants＇ age was between from 18 to 52, with a mean age of 29 years. The materials were 45 words of true and false cognate＇s words from 500 words by doing CVR （content validity ratio） and CVI （content validity index） （Lawshe＇s table with the index of 88% and 82% respectively） for being reliable and valid. ANOVA （Analysis of variance） was used for studying the effect of educational level on the rate of awareness. In other words, the awareness level has been compared in different educational levels. The results of Dankan test show that： There is no significant difference between the awareness of A.D., B.A., and M.A. levels, but there is a significant difference between the level of awareness of teachers group and the other groups （in true cognate words） and for false cognate words, there is no significant difference between the level of awareness of the four groups in this regard.

Multiple templates-based homology modeling and docking analysis of angiotensin Ⅱ type 1 receptor

Using the latest reported homologous Chemokine receptors （PDB ID： 3ODU, 3OE0 and 3OE6） as templates, twenty models of angiotensin II （Ang II） type 1 （AT1） receptor （known as p30556） were generated by multiple templates homology modeling. According to the results of the initial validation of these twenty models, the model 0020 was finally chosen as the best one for further studies. Then, a 2 ns molecular dynamic （MD） simulation for model 0020 was conducted in normal saline （0.9%, w/F） under periodical boundary conditions, which was followed by docking studies of model 0020 with several existing AT1 receptor blockers （ARBs）. The docking results reveal that model 0020 possesses good affinities with these docked ARBs which are in accordance with both the IC50 inhibitor values and their curative effects. The results also show more potent interactions between the model 0020 and its ARBs than those of ever reported results, such as hydrogen bonds, hydrophobic interactions, and especially cation-n interactions and π-π interactions which have never been reported before. This may reveal that the structure of the model 0020 is quite close to its real crystal structure and the model 0020 may have the potential to be used for structure based drug design：

Isotopic Model Estimate of Relative Contribution of Potential Water Pools to Water Uptake of Pinus sylvestris var. mongolica in Horqin Sandy Land

We examined stable isotope signals of precipitation, soil water, and xylem water and ran the multi-source linear mixing model （IsoSource） to determine water uptake depths and estimate proportional contribution of possible water pools to the water use of Mongolian pine （Pinus sylvestris var. mongolica） plantation in southeast Horqin Sandy Land. We also examined variations of the water use by Mongolian pine trees before and after a heavy precipitation event. The closeness of isotopic composition between xylem water and potential water pools presented that most of water uptake by the trees occurred in the depth of below 20 cm soil （up to 80 cm in this study）. Estimate from the IsoSource model agrees well with observation, and the model yielded that over 60% of the water was derived from 20–80 cm soil layer under relatively higher soil moisture conditions, contribution from much deeper soil depth may increase when the soil in this layer became dry. The contribution from the groundwater was very low since water table was much deeper than rooting depth of the trees. Isotopic signals of xylem water of Mongolian pine trees before and after a heavy precipitation of 14.4 mm on July 13 in 2009 exhibited that the trees could sense and use recent rain-charged soil water at the upper 20 cm soil layer 36 hours after the rain, and this contribution decreased rapidly in the following 24 hours. The ability of accessing different water pools of Mongolian pine trees under various soil moisture conditions is likely a good indicator of their adaptability to dry habitats in sandy lands.

关于中渡城的“琵琶行”与“长恨歌”

关于中渡城的“琵琶行”与“长恨歌”黄德读《文史春秋》１９９４年第６期吴狄同志的中渡城的＂琵琶行＂与＂长恨歌＂一文，兴味复萌，颇引为佳趣。忆梁立亚所作的咏女史李淑青遗事诗初问世时，曾不胫而走，广为传诵，不仅中渡、桂林一带，且遍及广西各地，从闹市以至穷乡...

Water use strategy of Ammopiptanthus mongolicus community in a drought year on the Mongolian Plateau

Aims In desert ecosystems,water is a restricting factor for plant growth and vegetation dynamics.The relatively stable water source from deep soil profile or groundwater is important for plant survival during drought.Understanding water use strategy of endangered species,in desert ecosystem is essential for their conservation and restoration such as Ammopiptanthus mongolicus on the Mongolian Plateau.Methods The stable isotope method ofδD andδ^(18)O was used to examine the main water sources of A.mongolicus and two companion shrubs,e.g.Artemisia ordosica and Artemisia xerophytica.The contribution of different water sources to each species was calculated by IsoSource model.Leafδ13C was used to compare long-term water use efficiency of three shrubs.Soil moisture and root distribution of three shrubs was measured to explain plant water use strategy.Important Findings The results showed that A.mongolicus relied on groundwater and 150–200 cm deep soil water,with the former contributing to almost half of its total water source.Artemisia ordosica mainly used 150–200 cm deep soil water,but also used shallow soil water within 100 cm in summer and autumn.Artemisia xerophytica mainly used 150–200 cm deep soil water and groundwater,with the latter contributing to about 30%–60%of its total water source.The three shrubs had dimorphic or deep root systems,which are in accord with their water sources.The WUE in the evergreen shrub A.mongolicus was higher than in two deciduous Artemisia shrubs,which may be an adaptive advantage in desert ecosystem.Therefore,groundwater is an important water source for the endangered shrub A.mongolicus in a drought year on Mongolian Plateau.Ammopiptanthus mongolicus and two Artemisia shrubs competed for deep soil water and groundwater.

Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice

The class III homeodomain-leucine zipper(HD-Zip III)gene family plays important roles in plant growth and development,including regulation of apical embryo patterning,embryonic shoot meristem formation,leaf polarity,vascular development,and meristem function,with a particularly crucial function in leaf development.Although HD-Zip III members are highly conserved in land plants,previous studies,such as genetic analyses based on multiple mutants in Arabidopsis and other plants,suggest that various HD-Zip III family genes have evolved with distinct functions and pleiotropic effects on plant growth and development.In this study,we analyzed a HD-Zip III member,OsHox33,and demonstrated that it plays an important role in age-dependent leaf senescence in rice.We constructed two specific RNAi vectors derived from the 5′-end region and 3′-UTR of OsHox33 to knockdown its expression.Transgenic plants harboring either RNAi construct displayed similar phenotypes of precocious leaf senescence symptoms,suggesting that knockdown of OsHox33 accelerates leaf senescence in rice.pOsHox33::GUS fusion expression and RT-PCR revealed that OsHox33 is highly expressed in young organs,especially in young meristems such as shoot apical meristems,intercalary meristems,and young callus.In addition,real-time PCR indicated that OsHox33 was more highly expressed in young leaves than in old leaves.To further investigate OsHox33 function,we analyzed chloroplast ultrastructure in different-aged leaves of RNAi plants,and found that OsHox33 knockdown accelerated chloroplast degradation,which is consistent with RNAi phenotypes.Finally,real-time PCR studies showed that OsHox33 can regulate the expression of GS1 and GS2,two senescence-associated genes.Taken together,the work presented here provides new insights into the function of HD-Zip III members in plants.

Autotetraploidy induced in Nianmaohuangqin (Radix Scutellariae viscidulae) with colchicine in vitro

OBJECTIVE： To establish and optimize the propaga- tion of Nianmaohuangqin （Radix Scutellariae Viscid- ulae） and induce and characterize polyploidy of Nianmaohuangqin （Radix Scutellariae Viscidulae）. METHODS： Buds from germinating seed-derived explants were induced by tissue culture. With an or- thogonal test, different concentrations of 6-benzyl- aminopurine （BAP）, indole-3-acetic acid （IAA） and kinetin （KT） were used to determine the optimal concentrations for the propagation of Nianmaohuangqin （Radix Scutellariae Viscidulae）. The differ- ent concentrations of IAA and rooting powder （ABT） were used to induce rooting. A 0.3% w/v col- chicine solution was used to induce polyploidy and the induced buds was identified by root-tip chromosome determination and stomatal apparatus ob- servation. RESULTS： A large number of buds could be in- duced directly from epicotyl and hypocotyl ex- plants on Murashige and Skoog （MS） （Murashige and Skoog 1962） medium supplemented with 1.1-1.3 mg/L BAP and 0.2 mg/L IAA. Root induction and development could be observed within 20 days of inoculation on 1/2 MS medium supplemented with 0.2 mg/L IAA and 0.1 mg/L ABTo Furthermore, 27 lines of autotetraploid individuals were ob- tained with a plantlet chromosome number of 2n= 4x=36. CONCLUSION： Autotetraploid lines could be ob- tained through induction with colchicine in vitro, proving that this method might be used for plant selection and breeding.

The water content and hydrogen isotope composition of continental lithospheric mantle and mantle-derived mafic igneous rocks in eastern China

The water contents of minerals and whole-rock in mantle-derived xenoliths from eastern China exhibit large variations and are generally lower than those from other on- and off-craton lithotectonic units. Nevertheless, the water contents of mineral and whole-rock in Junan peridotite xenoliths, which sourced from the juvenile lithospheric mantle, are generally higher than those elsewhere in eastern China. This suggests that the initial water content of juvenile lithospheric mantle is not low. There is no obvious correlation between the water contents and Mg~# values of minerals in the mantle xenoliths and no occurrence of diffusion profile in pyroxene, suggesting no relationship between the low water content of mantle xenolith and the diffusion loss of water during xenolith ascent with host basaltic magmas. If the subcontinental lithospheric mantle（SCLM） base is heated by the asthenospheric mantle, the diffusion loss of water is expected to occur. On the other hand, extraction of basaltic melts from the SCLM is a more efficient mechanism to reduce the water content of xenoliths. The primary melts of Mesozoic and Cenozoic basalts in eastern China have water contents, as calculated from the water contents of phenocrysts, higher than those of normal mid-ocean ridge basalts（MORB）. The Mesozoic basalts exhibit similar water contents to those of island arc basalts, whereas the Cenozoic basalts exhibit comparable water contents to oceanic island basalts and backarc basin basalts with some of them resembling island arc basalts. These observations suggest the water enrichment in the mantle source of continental basalts due to metasomatism by aqueous fluids and hydrous melts derived from dehydration and melting of deeply subducted crust. Mantle-derived megacrysts, minerals in xenoliths and phenocrysts in basalts from eastern China also exhibit largely variable hydrogen isotope compositions, indicating a large isotopic heterogeneity for the Cenozoic SCLM in eastern China. The water content that is higher than that of depleted MORB mantle and the hydrogen isotope composition that is deviated from that of depleted MORB mantle suggest that the Cenozoic continental lithospheric mantle suffered the metasomatism by hydrous melts derived from partial melting of the subducted Pacific slab below eastern China continent. The metasomatism would lead to the increase of water content in the SCLM base and then to the decrease of its viscosity. As a consequence, the SCLM base would be weakened and thus susceptible to tectonic erosion and delamination. As such, the crust-mantle interaction in oceanic subduction channel is the major cause for thinning of the craton lithosphere in North China.